Ontology type: schema:ScholarlyArticle
2012-02-20
AUTHORSAi-Sheng Xiong, Hai-Hua Jiang, Jing Zhuang, Ri-He Peng, Xiao-Fen Jin, Bo Zhu, Feng Wang, Jian Zhang, Quan-Hong Yao
ABSTRACTOne of the most rapid and effective defensive mechanisms plants have for protecting themselves, from a variety of biotic and abiotic stresses, is the regulation of plant signal transcription factors. AP2/ERF factors play an important role in plant development as well as in hormonal regulation and cold response. Directed evolution is a powerful tool to modify proteins, improving their properties, and for studying their structure–function relations. Here, the transgenic Arabidopsis plants over-expressed a mutant gene, BnaERF-B3-hy15-mu3, which encoded for a factor that exhibited more binding activity with the GCC box element than the wild-type gene BnaERF-B3-hy15 encode factor, and exhibited more freezing tolerance than transgenic plants containing the original BnaERF-B3-hy15 gene. Real-time PCR analyses also revealed that the expression levels of several stress-regulated genes were altered in the over-expressed BnaERF-B3-hy15-mu3 transgenic lines. The BnaERF-B3-hy15 responded to exogenous ABA. Using RT-PCR analysis, the expression of BnaERF-B3-hy15 at different stages and stress treatments were also analyzed. More... »
PAGES198-206
http://scigraph.springernature.com/pub.10.1007/s12033-012-9515-x
DOIhttp://dx.doi.org/10.1007/s12033-012-9515-x
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PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/22351429
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