An Improved Protocol for the Isolation of RNA from Roots of Tea (Camellia sinensis (L.) O. Kuntze) View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2012-09

AUTHORS

Richard Chalo Muoki, Asosii Paul, Anita Kumari, Kashmir Singh, Sanjay Kumar

ABSTRACT

Tea, a beverage crop, is a rich source of polyphenols and polysaccharides which greatly attribute to its importance. However, oxidation and precipitation of these compounds during nucleic acids extraction is a limitation to molecular biology and genomic studies. On isolation of total RNA from root tissue using established protocols, difficulties were encountered in terms of purity and quantity of isolated RNA or some of the methods were time-consuming and also yields were low. The present communication combines a phenol-based RNA isolation protocol with a cetyltrimethylammonium bromide-based procedure with appropriate modifications. This protocol successfully isolated RNA from tap root tissue in 2-3 h as compared with 16 h reported by the previous method. Also, RNA yield was higher by more than fourfold. The RNA isolated by this protocol was successfully used for downstream applications such as RT-PCR and the construction of suppression subtractive hybridization library. The developed protocol worked well with other plant tissue with high polyphenols and polysaccharides contents. More... »

PAGES

82-88

References to SciGraph publications

  • 2011-06. Isolation of High-Quality RNA from Reaumuria soongorica, a Desert Plant Rich in Secondary Metabolites in MOLECULAR BIOTECHNOLOGY
  • 2005-03. Differential display-mediated identification of three drought-responsive expressed sequence tags in tea [Camellia sinensis (L.) O. Kuntze] in JOURNAL OF BIOSCIENCES
  • 2011-12. An RNA isolation system for plant tissues rich in secondary metabolites in BMC RESEARCH NOTES
  • 2006-12. An optimized grapevine RNA isolation procedure and statistical determination of reference genes for real-time RT-PCR during berry development in BMC PLANT BIOLOGY
  • 2012-04. Alpha-tubulin (CsTUA) up-regulated during winter dormancy is a low temperature inducible gene in tea [Camellia sinensis (L.) O. Kuntze] in MOLECULAR BIOLOGY REPORTS
  • 2011-12. Responses to winter dormancy, temperature, and plant hormones share gene networks in FUNCTIONAL & INTEGRATIVE GENOMICS
  • 1993-06. A simple and efficient method for isolating RNA from pine trees in PLANT MOLECULAR BIOLOGY REPORTER
  • 2006-12. The RIN: an RNA integrity number for assigning integrity values to RNA measurements in BMC MOLECULAR BIOLOGY
  • 2004-12. RNA extraction from different apple tissues rich in polyphenols and polysaccharides for cDNA library construction in PLANT MOLECULAR BIOLOGY REPORTER
  • 2005-05. Antioxidant activity of fractionated extracts of rhizomes of high-altitude Podophyllum hexandrum: Role in radiation protection in MOLECULAR AND CELLULAR BIOCHEMISTRY
  • 2011-10. Rapid and Efficient Isolation of High Quality Nucleic Acids from Plant Tissues Rich in Polyphenols and Polysaccharides in MOLECULAR BIOTECHNOLOGY
  • 2001-06. RNA isolation from high-phenolic tea leaves and apical buds in PLANT MOLECULAR BIOLOGY REPORTER
  • 1999-03. An Improved RNA Isolation Method for Plant Tissues Containing High Levels of Phenolic Compounds or Carbohydrates in PLANT MOLECULAR BIOLOGY REPORTER
  • 2004-02. Isolation of functional RNA from small amounts of different grape and apple tissues in MOLECULAR BIOTECHNOLOGY
  • 2009-05. p-Coumarate:CoA ligase as a key gene in the yield of catechins in tea [Camellia sinensis (L.) O. Kuntze] in FUNCTIONAL & INTEGRATIVE GENOMICS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s12033-011-9476-5

    DOI

    http://dx.doi.org/10.1007/s12033-011-9476-5

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/22144070


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