Agrobacterium-Mediated Silencing of Caffeine Synthesis through Root Transformation in Camellia sinensis L. View Full Text


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Article Info

DATE

2010-12-24

AUTHORS

Prashant Mohanpuria, Vinay Kumar, Paramvir Singh Ahuja, Sudesh Kumar Yadav

ABSTRACT

Tea [Camellia sinensis (L.) O. Kuntze] is a perennial and most popular non-alcoholic caffeine-containing beverage crop. Tea has several constraints for its genetic improvement such as its high polyphenolic content and woody perennial nature. The development of transgenic tea is very difficult, laborious, and time taking process. In tea, regeneration requires minimum 8–12 months. In view of this, attempt has been made in this article to develop a rapid, efficient, and quite economical Agrobacterium-mediated root transformation system for tea. The feasibility of the developed protocol has been documented through silencing caffeine biosynthesis. For this, one-month-old tea seedlings were exposed to fresh wounding at the elongation zone of roots and were inoculated with Agrobacterium tumefaciens cultures carrying a RNAi construct (pFGC1008-CS). The pFGC1008-CS contained 376 bp of caffeine synthase (CS) cDNA fragment in sense and antisense direction with an intron in between. This has made the RNAi construct to produce a hairpin RNA (ihpRNA). The suppressed expression of CS gene and a marked reduction in caffeine and theobromine contents in young shoots of tea seedlings were obtained after root transformation through Agrobacterium infiltration. Such transformation system could be useful for functional analysis of genes in tea like woody and perennial plants. More... »

PAGES

235-243

References to SciGraph publications

  • 2004-06. Agrobacterium tumefaciens-Mediated genetic transformation in tea (Camellia sinensis [L.] O. Kuntze) in PLANT MOLECULAR BIOLOGY REPORTER
  • 2005-06. TransientAgrobacterium-mediated gene expression in theArabidopsis hydroponics root system for subcellular localization studies in PLANT MOLECULAR BIOLOGY REPORTER
  • 2001-11-17. Transgenic tea [Camellia sinensis (L.) O. Kuntze cv. Kangra Jat] plants obtained by Agrobacterium-mediated transformation of somatic embryos in PLANT CELL REPORTS
  • 2009-05-29. Caffeine Biosynthesis and Degradation in Tea [Camellia sinensis (L.) O. Kuntze] is under Developmental and Seasonal Regulation in MOLECULAR BIOTECHNOLOGY
  • 1990-01. Localized transient expression of GUS in leaf discs following cocultivation with Agrobacterium in PLANT MOLECULAR BIOLOGY
  • 2002-07. RNA interference in NATURE
  • 2006-09-14. Agrobacterium-mediated genetic transformation of tea leaf explants: effects of counteracting bactericidity of leaf polyphenols without loss of bacterial virulence in PLANT CELL REPORTS
  • 1990-09. Agrobacterium tumefaciens-mediated transformation of Pisum sativum L. using binary and cointegrate vectors in PLANT CELL REPORTS
  • 2004-03. Recent Advances of Tea (Camellia Sinensis) Biotechnology in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 2010-04-30. Tea caffeine: Metabolism, functions, and reduction strategies in FOOD SCIENCE AND BIOTECHNOLOGY
  • 2004-03. A rapidAgrobacterium-mediatedArabidopsis thaliana transient assay system in PLANT MOLECULAR BIOLOGY REPORTER
  • 2008-06. Transient RNAi based gene silencing of glutathione synthetase reduces glutathione content in Camellia sinensis (L.) O. Kuntze somatic embryos in BIOLOGIA PLANTARUM
  • 2007-10-11. Towards Generating Caffeine-free Tea by Metabolic Engineering in PLANT FOODS FOR HUMAN NUTRITION
  • 2005-09. Agrobacterium tumefaciens-mediated transformation of embryogenic tissues of tea (Camellia sinensis (L.) O. Kuntze) in PLANT MOLECULAR BIOLOGY REPORTER
  • 2003-03. A simple and rapid method to detect plant siRNAs using nonradioactive probes in PLANT MOLECULAR BIOLOGY REPORTER
  • 1994-09. Production ofAgrobacterium-mediated transgenic fertile plants by direct somatic embryogenesis from immature zygotic embryos ofDatura innoxia in PLANT MOLECULAR BIOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s12033-010-9364-4

    DOI

    http://dx.doi.org/10.1007/s12033-010-9364-4

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/21181507


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