Molecular and Biochemical Characterization of an Endochitinase (ChiA-HD73) from Bacillus thuringiensis subsp. kurstaki HD-73 View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2007-12-14

AUTHORS

J. Eleazar Barboza-Corona, Dora M. Reyes-Rios, Rubén Salcedo-Hernández, Dennis K. Bideshi

ABSTRACT

An endochitinase gene (chiA-HD73) from the insecticidal bacterium Bacillus thuringiensis subsp. kurstaki HD-73 was cloned, sequenced, and expressed in Escherichia coli DH5αF′. The chitinase activity of the encoded protein was studied in assays with different fluorogenic substrates. The chiA-HD73 gene contained an open-reading frame that encoded an endochitinase with a deduced molecular weight and an isoelectric point of, respectively, 74.5 kDa and 5.75. A putative signal peptide with cleavage sites for both Gram-positive and Gram-negative bacteria was identified. Comparison of ChiA-HD73 with other chitinases revealed a modular structure composed of a catalytic domain and a putative chitin-binding domain. ChiA-HD73 hydrolyzed both tetrameric and trimeric fluorogenic substrates, but not a chitobiose analog substrate, suggesting that the activity of ChiA-HD73 is mainly endochitinolytic. In addition, ChiA-HD73 showed high enzymatic activity within a broad pH range (pH 4–10), with a peak activity at pH 6.5. The optimal temperature for enzymatic activity was observed at 55°C. Its activity in a broad range of temperatures and pH suggests ChiA-HD73 could have biotechnological applications in insect control, particularly in synergizing the insecticidal crystal protein toxins of B. thuringiensis. More... »

PAGES

29-37

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s12033-007-9025-4

DOI

http://dx.doi.org/10.1007/s12033-007-9025-4

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1004546938

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/18080218


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0605", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Microbiology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Amino Acid Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Bacillus thuringiensis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Catalytic Domain", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chitinases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cloning, Molecular", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hydrogen-Ion Concentration", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hydrolysis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Sequence Data", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Plasmids", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Recombinant Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sequence Alignment", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sequence Analysis, DNA", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sequence Homology, Amino Acid", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Temperature", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transformation, Genetic", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Instituto de Ciencias Agr\u00edcolas, Departamento de Ingenier\u00eda en Alimentos, Universidad de Guanajuato, Apartado postal 311, 36500, Irapuato, Guanajuato, Mexico", 
          "id": "http://www.grid.ac/institutes/grid.412891.7", 
          "name": [
            "Instituto de Ciencias Agr\u00edcolas, Departamento de Ingenier\u00eda en Alimentos, Universidad de Guanajuato, Apartado postal 311, 36500, Irapuato, Guanajuato, Mexico"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Barboza-Corona", 
        "givenName": "J. Eleazar", 
        "id": "sg:person.0745304467.10", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0745304467.10"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Colegio de Estudios Cient\u00edficos y Tecnol\u00f3gicos del Estado de Guanajuato, Plantel Irapuato Valle Verde, 36568, Irapuato, Guanajuato, Mexico", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Instituto de Ciencias Agr\u00edcolas, Departamento de Ingenier\u00eda en Alimentos, Universidad de Guanajuato, Apartado postal 311, 36500, Irapuato, Guanajuato, Mexico", 
            "Colegio de Estudios Cient\u00edficos y Tecnol\u00f3gicos del Estado de Guanajuato, Plantel Irapuato Valle Verde, 36568, Irapuato, Guanajuato, Mexico"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Reyes-Rios", 
        "givenName": "Dora M.", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Instituto de Ciencias Agr\u00edcolas, Departamento de Ingenier\u00eda en Alimentos, Universidad de Guanajuato, Apartado postal 311, 36500, Irapuato, Guanajuato, Mexico", 
          "id": "http://www.grid.ac/institutes/grid.412891.7", 
          "name": [
            "Instituto de Ciencias Agr\u00edcolas, Departamento de Ingenier\u00eda en Alimentos, Universidad de Guanajuato, Apartado postal 311, 36500, Irapuato, Guanajuato, Mexico"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Salcedo-Hern\u00e1ndez", 
        "givenName": "Rub\u00e9n", 
        "id": "sg:person.01306346767.18", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01306346767.18"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Entomology, University of California, 92521, Riverside, CA, USA", 
          "id": "http://www.grid.ac/institutes/grid.266097.c", 
          "name": [
            "Department of Natural and Mathematical Sciences, California Baptist University, 8432 Magnolia Avenue, 92504, Riverside, CA, USA", 
            "Department of Entomology, University of California, 92521, Riverside, CA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bideshi", 
        "givenName": "Dennis K.", 
        "id": "sg:person.01334024054.46", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01334024054.46"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1023/a:1005626208193", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050080055", 
          "https://doi.org/10.1023/a:1005626208193"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1385/mb:31:2:103", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027143880", 
          "https://doi.org/10.1385/mb:31:2:103"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/227680a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010419937", 
          "https://doi.org/10.1038/227680a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s002530100630", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015684619", 
          "https://doi.org/10.1007/s002530100630"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1023/b:bile.0000023021.50213.ed", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007957807", 
          "https://doi.org/10.1023/b:bile.0000023021.50213.ed"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10482-006-9127-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049958306", 
          "https://doi.org/10.1007/s10482-006-9127-1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-1-4613-2167-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047062099", 
          "https://doi.org/10.1007/978-1-4613-2167-5"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2007-12-14", 
    "datePublishedReg": "2007-12-14", 
    "description": "An endochitinase gene (chiA-HD73) from the insecticidal bacterium Bacillus thuringiensis subsp. kurstaki HD-73 was cloned, sequenced, and expressed in Escherichia coli DH5\u03b1F\u2032. The chitinase activity of the encoded protein was studied in assays with different fluorogenic substrates. The chiA-HD73 gene contained an open-reading frame that encoded an endochitinase with a deduced molecular weight and an isoelectric point of, respectively, 74.5\u00a0kDa and 5.75. A putative signal peptide with cleavage sites for both Gram-positive and Gram-negative bacteria was identified. Comparison of ChiA-HD73 with other chitinases revealed a modular structure composed of a catalytic domain and a putative chitin-binding domain. ChiA-HD73 hydrolyzed both tetrameric and trimeric fluorogenic substrates, but not a chitobiose analog substrate, suggesting that the activity of ChiA-HD73 is mainly endochitinolytic. In addition, ChiA-HD73 showed high enzymatic activity within a broad pH range (pH\u00a04\u201310), with a peak activity at pH\u00a06.5. The optimal temperature for enzymatic activity was observed at 55\u00b0C. Its activity in a broad range of temperatures and pH suggests ChiA-HD73 could have biotechnological applications in insect control, particularly in synergizing the insecticidal crystal protein toxins of B.\u00a0thuringiensis.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/s12033-007-9025-4", 
    "inLanguage": "en", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1106201", 
        "issn": [
          "1073-6085", 
          "1559-0305"
        ], 
        "name": "Molecular Biotechnology", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "39"
      }
    ], 
    "keywords": [
      "Bacillus thuringiensis subsp", 
      "kurstaki HD-73", 
      "thuringiensis subsp", 
      "HD-73", 
      "putative chitin-binding domain", 
      "deduced molecular weight", 
      "putative signal peptide", 
      "different fluorogenic substrates", 
      "enzymatic activity", 
      "open-reading frame", 
      "chitin-binding domain", 
      "fluorogenic substrate", 
      "Gram-negative bacteria", 
      "endochitinase gene", 
      "signal peptide", 
      "catalytic domain", 
      "biotechnological applications", 
      "insect control", 
      "biochemical characterization", 
      "protein toxins", 
      "chitinase activity", 
      "cleavage site", 
      "high enzymatic activity", 
      "endochitinase", 
      "genes", 
      "isoelectric point", 
      "optimal temperature", 
      "subsp", 
      "analogue substrates", 
      "broad pH range", 
      "modular structure", 
      "thuringiensis", 
      "chitinases", 
      "molecular weight", 
      "domain", 
      "protein", 
      "substrate", 
      "activity", 
      "bacteria", 
      "peak activity", 
      "Gram", 
      "broad range", 
      "assays", 
      "toxin", 
      "molecular", 
      "peptides", 
      "sites", 
      "pH range", 
      "characterization", 
      "frame", 
      "structure", 
      "addition", 
      "range", 
      "control", 
      "weight", 
      "comparison", 
      "temperature", 
      "applications", 
      "point", 
      "insecticidal bacterium Bacillus thuringiensis subsp", 
      "bacterium Bacillus thuringiensis subsp", 
      "Escherichia coli DH5\u03b1F\u2032.", 
      "coli DH5\u03b1F\u2032.", 
      "DH5\u03b1F\u2032.", 
      "chiA-HD73 gene", 
      "ChiA-HD73", 
      "trimeric fluorogenic substrates", 
      "chitobiose analog substrate", 
      "insecticidal crystal protein toxins", 
      "crystal protein toxins"
    ], 
    "name": "Molecular and Biochemical Characterization of an Endochitinase (ChiA-HD73) from Bacillus thuringiensis subsp. kurstaki HD-73", 
    "pagination": "29-37", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1004546938"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s12033-007-9025-4"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "18080218"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s12033-007-9025-4", 
      "https://app.dimensions.ai/details/publication/pub.1004546938"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2021-12-01T19:18", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20211201/entities/gbq_results/article/article_446.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/s12033-007-9025-4"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s12033-007-9025-4'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s12033-007-9025-4'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s12033-007-9025-4'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s12033-007-9025-4'


 

This table displays all metadata directly associated to this object as RDF triples.

248 TRIPLES      22 PREDICATES      118 URIs      103 LITERALS      22 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s12033-007-9025-4 schema:about N3e64c72a8f42413695833e34962be654
2 N51dfa78acbd64e8ba60d46a8ea667430
3 N5b6e3a2929e546d0a10f7412ab318f5b
4 N62790e8177dd4fe88099ff24eb111e7f
5 N6533a30581c44a74a8e5dfe5dee90991
6 N7a28aa9e04d44c24a779e2a407869974
7 N83b7b6d8bd414a93b0089ab91b923321
8 N89b9634b80a04f309d5e3c44438f5b81
9 Ncc31ac6589d04f2f831236bf5bc82a5f
10 Nd6710b866dc9410c9c6c4ee96dfae090
11 Ne874a37a644b423aa47813e65acefa69
12 Nece9c8a9a6d043138848fe2b6b208519
13 Nf09d89a17e08434193f2869259f4cc89
14 Nf9a46fd193024c75b21b8c56078d5c5e
15 Nfe9d80965247435db4974825a5a36752
16 anzsrc-for:06
17 anzsrc-for:0605
18 schema:author N7bda4a6c6b1446bcb80a8e80beb46200
19 schema:citation sg:pub.10.1007/978-1-4613-2167-5
20 sg:pub.10.1007/s002530100630
21 sg:pub.10.1007/s10482-006-9127-1
22 sg:pub.10.1023/a:1005626208193
23 sg:pub.10.1023/b:bile.0000023021.50213.ed
24 sg:pub.10.1038/227680a0
25 sg:pub.10.1385/mb:31:2:103
26 schema:datePublished 2007-12-14
27 schema:datePublishedReg 2007-12-14
28 schema:description An endochitinase gene (chiA-HD73) from the insecticidal bacterium Bacillus thuringiensis subsp. kurstaki HD-73 was cloned, sequenced, and expressed in Escherichia coli DH5αF′. The chitinase activity of the encoded protein was studied in assays with different fluorogenic substrates. The chiA-HD73 gene contained an open-reading frame that encoded an endochitinase with a deduced molecular weight and an isoelectric point of, respectively, 74.5 kDa and 5.75. A putative signal peptide with cleavage sites for both Gram-positive and Gram-negative bacteria was identified. Comparison of ChiA-HD73 with other chitinases revealed a modular structure composed of a catalytic domain and a putative chitin-binding domain. ChiA-HD73 hydrolyzed both tetrameric and trimeric fluorogenic substrates, but not a chitobiose analog substrate, suggesting that the activity of ChiA-HD73 is mainly endochitinolytic. In addition, ChiA-HD73 showed high enzymatic activity within a broad pH range (pH 4–10), with a peak activity at pH 6.5. The optimal temperature for enzymatic activity was observed at 55°C. Its activity in a broad range of temperatures and pH suggests ChiA-HD73 could have biotechnological applications in insect control, particularly in synergizing the insecticidal crystal protein toxins of B. thuringiensis.
29 schema:genre article
30 schema:inLanguage en
31 schema:isAccessibleForFree false
32 schema:isPartOf N6a089e4d978b497ca27039a26c7fca5a
33 Nef7864aa7d87440e80376893795de737
34 sg:journal.1106201
35 schema:keywords Bacillus thuringiensis subsp
36 ChiA-HD73
37 DH5αF′.
38 Escherichia coli DH5αF′.
39 Gram
40 Gram-negative bacteria
41 HD-73
42 activity
43 addition
44 analogue substrates
45 applications
46 assays
47 bacteria
48 bacterium Bacillus thuringiensis subsp
49 biochemical characterization
50 biotechnological applications
51 broad pH range
52 broad range
53 catalytic domain
54 characterization
55 chiA-HD73 gene
56 chitin-binding domain
57 chitinase activity
58 chitinases
59 chitobiose analog substrate
60 cleavage site
61 coli DH5αF′.
62 comparison
63 control
64 crystal protein toxins
65 deduced molecular weight
66 different fluorogenic substrates
67 domain
68 endochitinase
69 endochitinase gene
70 enzymatic activity
71 fluorogenic substrate
72 frame
73 genes
74 high enzymatic activity
75 insect control
76 insecticidal bacterium Bacillus thuringiensis subsp
77 insecticidal crystal protein toxins
78 isoelectric point
79 kurstaki HD-73
80 modular structure
81 molecular
82 molecular weight
83 open-reading frame
84 optimal temperature
85 pH range
86 peak activity
87 peptides
88 point
89 protein
90 protein toxins
91 putative chitin-binding domain
92 putative signal peptide
93 range
94 signal peptide
95 sites
96 structure
97 subsp
98 substrate
99 temperature
100 thuringiensis
101 thuringiensis subsp
102 toxin
103 trimeric fluorogenic substrates
104 weight
105 schema:name Molecular and Biochemical Characterization of an Endochitinase (ChiA-HD73) from Bacillus thuringiensis subsp. kurstaki HD-73
106 schema:pagination 29-37
107 schema:productId Nbb3cf1fdac97476db30fbd9f2d29d630
108 Nc4ea1a38086f44f1bdece69f600d9a9b
109 Nfafc6d07db424063ad5b5609a46b02e1
110 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004546938
111 https://doi.org/10.1007/s12033-007-9025-4
112 schema:sdDatePublished 2021-12-01T19:18
113 schema:sdLicense https://scigraph.springernature.com/explorer/license/
114 schema:sdPublisher N27638eb9fe8d409c8a0ffccd9912db9f
115 schema:url https://doi.org/10.1007/s12033-007-9025-4
116 sgo:license sg:explorer/license/
117 sgo:sdDataset articles
118 rdf:type schema:ScholarlyArticle
119 N0adbea95c034443cbcbb44a2ee807cdf rdf:first N6d25b89cc5d041a19ba67bf63f83f466
120 rdf:rest N7ac2ac41490747cb992f3f6b5c7ee149
121 N27638eb9fe8d409c8a0ffccd9912db9f schema:name Springer Nature - SN SciGraph project
122 rdf:type schema:Organization
123 N3e64c72a8f42413695833e34962be654 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
124 schema:name Temperature
125 rdf:type schema:DefinedTerm
126 N51dfa78acbd64e8ba60d46a8ea667430 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
127 schema:name Transformation, Genetic
128 rdf:type schema:DefinedTerm
129 N5b6e3a2929e546d0a10f7412ab318f5b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
130 schema:name Hydrogen-Ion Concentration
131 rdf:type schema:DefinedTerm
132 N62790e8177dd4fe88099ff24eb111e7f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
133 schema:name Sequence Analysis, DNA
134 rdf:type schema:DefinedTerm
135 N6533a30581c44a74a8e5dfe5dee90991 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
136 schema:name Chitinases
137 rdf:type schema:DefinedTerm
138 N6a089e4d978b497ca27039a26c7fca5a schema:volumeNumber 39
139 rdf:type schema:PublicationVolume
140 N6d25b89cc5d041a19ba67bf63f83f466 schema:affiliation grid-institutes:None
141 schema:familyName Reyes-Rios
142 schema:givenName Dora M.
143 rdf:type schema:Person
144 N7a28aa9e04d44c24a779e2a407869974 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
145 schema:name Sequence Alignment
146 rdf:type schema:DefinedTerm
147 N7ac2ac41490747cb992f3f6b5c7ee149 rdf:first sg:person.01306346767.18
148 rdf:rest Nc03868695b8a44a0a70791612d861e89
149 N7bda4a6c6b1446bcb80a8e80beb46200 rdf:first sg:person.0745304467.10
150 rdf:rest N0adbea95c034443cbcbb44a2ee807cdf
151 N83b7b6d8bd414a93b0089ab91b923321 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
152 schema:name Catalytic Domain
153 rdf:type schema:DefinedTerm
154 N89b9634b80a04f309d5e3c44438f5b81 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
155 schema:name Sequence Homology, Amino Acid
156 rdf:type schema:DefinedTerm
157 Nbb3cf1fdac97476db30fbd9f2d29d630 schema:name doi
158 schema:value 10.1007/s12033-007-9025-4
159 rdf:type schema:PropertyValue
160 Nc03868695b8a44a0a70791612d861e89 rdf:first sg:person.01334024054.46
161 rdf:rest rdf:nil
162 Nc4ea1a38086f44f1bdece69f600d9a9b schema:name dimensions_id
163 schema:value pub.1004546938
164 rdf:type schema:PropertyValue
165 Ncc31ac6589d04f2f831236bf5bc82a5f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
166 schema:name Recombinant Proteins
167 rdf:type schema:DefinedTerm
168 Nd6710b866dc9410c9c6c4ee96dfae090 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
169 schema:name Cloning, Molecular
170 rdf:type schema:DefinedTerm
171 Ne874a37a644b423aa47813e65acefa69 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
172 schema:name Amino Acid Sequence
173 rdf:type schema:DefinedTerm
174 Nece9c8a9a6d043138848fe2b6b208519 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
175 schema:name Molecular Sequence Data
176 rdf:type schema:DefinedTerm
177 Nef7864aa7d87440e80376893795de737 schema:issueNumber 1
178 rdf:type schema:PublicationIssue
179 Nf09d89a17e08434193f2869259f4cc89 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
180 schema:name Bacillus thuringiensis
181 rdf:type schema:DefinedTerm
182 Nf9a46fd193024c75b21b8c56078d5c5e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
183 schema:name Plasmids
184 rdf:type schema:DefinedTerm
185 Nfafc6d07db424063ad5b5609a46b02e1 schema:name pubmed_id
186 schema:value 18080218
187 rdf:type schema:PropertyValue
188 Nfe9d80965247435db4974825a5a36752 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
189 schema:name Hydrolysis
190 rdf:type schema:DefinedTerm
191 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
192 schema:name Biological Sciences
193 rdf:type schema:DefinedTerm
194 anzsrc-for:0605 schema:inDefinedTermSet anzsrc-for:
195 schema:name Microbiology
196 rdf:type schema:DefinedTerm
197 sg:journal.1106201 schema:issn 1073-6085
198 1559-0305
199 schema:name Molecular Biotechnology
200 schema:publisher Springer Nature
201 rdf:type schema:Periodical
202 sg:person.01306346767.18 schema:affiliation grid-institutes:grid.412891.7
203 schema:familyName Salcedo-Hernández
204 schema:givenName Rubén
205 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01306346767.18
206 rdf:type schema:Person
207 sg:person.01334024054.46 schema:affiliation grid-institutes:grid.266097.c
208 schema:familyName Bideshi
209 schema:givenName Dennis K.
210 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01334024054.46
211 rdf:type schema:Person
212 sg:person.0745304467.10 schema:affiliation grid-institutes:grid.412891.7
213 schema:familyName Barboza-Corona
214 schema:givenName J. Eleazar
215 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0745304467.10
216 rdf:type schema:Person
217 sg:pub.10.1007/978-1-4613-2167-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047062099
218 https://doi.org/10.1007/978-1-4613-2167-5
219 rdf:type schema:CreativeWork
220 sg:pub.10.1007/s002530100630 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015684619
221 https://doi.org/10.1007/s002530100630
222 rdf:type schema:CreativeWork
223 sg:pub.10.1007/s10482-006-9127-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049958306
224 https://doi.org/10.1007/s10482-006-9127-1
225 rdf:type schema:CreativeWork
226 sg:pub.10.1023/a:1005626208193 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050080055
227 https://doi.org/10.1023/a:1005626208193
228 rdf:type schema:CreativeWork
229 sg:pub.10.1023/b:bile.0000023021.50213.ed schema:sameAs https://app.dimensions.ai/details/publication/pub.1007957807
230 https://doi.org/10.1023/b:bile.0000023021.50213.ed
231 rdf:type schema:CreativeWork
232 sg:pub.10.1038/227680a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010419937
233 https://doi.org/10.1038/227680a0
234 rdf:type schema:CreativeWork
235 sg:pub.10.1385/mb:31:2:103 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027143880
236 https://doi.org/10.1385/mb:31:2:103
237 rdf:type schema:CreativeWork
238 grid-institutes:None schema:alternateName Colegio de Estudios Científicos y Tecnológicos del Estado de Guanajuato, Plantel Irapuato Valle Verde, 36568, Irapuato, Guanajuato, Mexico
239 schema:name Colegio de Estudios Científicos y Tecnológicos del Estado de Guanajuato, Plantel Irapuato Valle Verde, 36568, Irapuato, Guanajuato, Mexico
240 Instituto de Ciencias Agrícolas, Departamento de Ingeniería en Alimentos, Universidad de Guanajuato, Apartado postal 311, 36500, Irapuato, Guanajuato, Mexico
241 rdf:type schema:Organization
242 grid-institutes:grid.266097.c schema:alternateName Department of Entomology, University of California, 92521, Riverside, CA, USA
243 schema:name Department of Entomology, University of California, 92521, Riverside, CA, USA
244 Department of Natural and Mathematical Sciences, California Baptist University, 8432 Magnolia Avenue, 92504, Riverside, CA, USA
245 rdf:type schema:Organization
246 grid-institutes:grid.412891.7 schema:alternateName Instituto de Ciencias Agrícolas, Departamento de Ingeniería en Alimentos, Universidad de Guanajuato, Apartado postal 311, 36500, Irapuato, Guanajuato, Mexico
247 schema:name Instituto de Ciencias Agrícolas, Departamento de Ingeniería en Alimentos, Universidad de Guanajuato, Apartado postal 311, 36500, Irapuato, Guanajuato, Mexico
248 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...