Expression Profiling of Notch Signalling Pathway and Gamma-Secretase Activity in the Brain of Ts1Cje Mouse Model of Down Syndrome View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2019-04

AUTHORS

Hadri Hadi Yusof, Han-Chung Lee, Eryse Amira Seth, Xiangzhong Wu, Chelsee A. Hewitt, Hamish S Scott, Pike-See Cheah, Yue-Ming Li, De-Ming Chau, King-Hwa Ling

ABSTRACT

Notch signalling pathway is involved in the proliferation of neural progenitor cells (NPCs), to inhibit neuronal cell commitment and to promote glial cell fate. Notch protein is cleaved by gamma-secretase, a multisubunit transmembrane protein complex that releases the Notch intracellular domain (NICD) and subsequently activates the downstream targets. Down syndrome (DS) individuals exhibit an increased number of glial cells (particularly astrocytes), and reduced number of neurons suggesting the involvement of Notch signalling pathway in the neurogenic-to-gliogenic shift in DS brain. Ts1Cje is a DS mouse model that exhibit similar neuropathology to human DS individuals. To date, the spatiotemporal gene expression of the Notch and gamma-secretase genes have not been characterised in Ts1Cje mouse brain. Understanding the expression pattern of Notch and gamma-secretase genes may provide a better understanding of the underlying mechanism that leads to the shift. Gene expression analysis using RT-qPCR was performed on early embryonic and postnatal development of DS brain. In the developing mouse brain, mRNA expression analysis showed that gamma-secretase members (Psen1, Pen-2, Aph-1b, and Ncstn) were not differentially expressed. Notch2 was found to be downregulated in the developing Ts1Cje brain samples. Postnatal gene expression study showed complex expression patterns and Notch1 and Notch2 genes were found to be significantly downregulated in the hippocampus at postnatal day 30. Results from RT-qPCR analysis from E15.5 neurosphere culture showed an increase of expression of Psen1, and Aph-1b but downregulation of Pen-2 and Ncstn genes. Gamma-secretase activity in Ts1Cje E15.5 neurospheres was significantly increased by fivefold. In summary, the association and the role of Notch and gamma-secretase gene expression throughout development with neurogenic-to-gliogenic shift in Ts1Cje remain undefined and warrant further validation. More... »

PAGES

1-11

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s12031-019-01275-2

DOI

http://dx.doi.org/10.1007/s12031-019-01275-2

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1112096426

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30758748


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Universiti Putra Malaysia", 
          "id": "https://www.grid.ac/institutes/grid.11142.37", 
          "name": [
            "Genetics & Regenerative Medicine Research Centre (GRMRC), Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia", 
            "Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yusof", 
        "givenName": "Hadri Hadi", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Universiti Putra Malaysia", 
          "id": "https://www.grid.ac/institutes/grid.11142.37", 
          "name": [
            "Genetics & Regenerative Medicine Research Centre (GRMRC), Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia", 
            "Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lee", 
        "givenName": "Han-Chung", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Universiti Putra Malaysia", 
          "id": "https://www.grid.ac/institutes/grid.11142.37", 
          "name": [
            "Genetics & Regenerative Medicine Research Centre (GRMRC), Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia", 
            "Department of Human Anatomy, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Seth", 
        "givenName": "Eryse Amira", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Memorial Sloan Kettering Cancer Center", 
          "id": "https://www.grid.ac/institutes/grid.51462.34", 
          "name": [
            "Chemical Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wu", 
        "givenName": "Xiangzhong", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Peter MacCallum Cancer Centre", 
          "id": "https://www.grid.ac/institutes/grid.1055.1", 
          "name": [
            "Department of Pathology, The Peter MacCallum Cancer Centre, East Melbourne, Victoria, Australia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hewitt", 
        "givenName": "Chelsee A.", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Centre for Cancer Biology", 
          "id": "https://www.grid.ac/institutes/grid.470344.0", 
          "name": [
            "Department of Genetics and Molecular Pathology, Centre for Cancer Biology, An Alliance Between SA Pathology and the University of South Australia, SA Pathology, Adelaide, Australia", 
            "School of Medicine, Faculty of Health Sciences, University of Adelaide, Adelaide, SA, Australia", 
            "School of Pharmacy and Medical Science, University of South Australia, Adelaide, Australia", 
            "School of Biological Sciences, University of Adelaide, Adelaide, Australia", 
            "Australian Cancer Research Foundation Genomics Facility, Centre for Cancer Biology, SA Pathology, Adelaide, Australia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Scott", 
        "givenName": "Hamish S", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Universiti Putra Malaysia", 
          "id": "https://www.grid.ac/institutes/grid.11142.37", 
          "name": [
            "Genetics & Regenerative Medicine Research Centre (GRMRC), Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia", 
            "Department of Human Anatomy, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Cheah", 
        "givenName": "Pike-See", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Memorial Sloan Kettering Cancer Center", 
          "id": "https://www.grid.ac/institutes/grid.51462.34", 
          "name": [
            "Chemical Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Li", 
        "givenName": "Yue-Ming", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Universiti Putra Malaysia", 
          "id": "https://www.grid.ac/institutes/grid.11142.37", 
          "name": [
            "Genetics & Regenerative Medicine Research Centre (GRMRC), Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia", 
            "Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Chau", 
        "givenName": "De-Ming", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Universiti Putra Malaysia", 
          "id": "https://www.grid.ac/institutes/grid.11142.37", 
          "name": [
            "Genetics & Regenerative Medicine Research Centre (GRMRC), Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia", 
            "Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ling", 
        "givenName": "King-Hwa", 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1093/emboj/18.8.2196", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000982033"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1523/jneurosci.4767-10.2011", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001743661"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/(sici)1097-4695(199912)41:4<524::aid-neu8>3.0.co;2-i", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007500182"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/cercor/bhp176", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010057857"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0011561", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010538191"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1242/dev.063610", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010560245"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m111.300483", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011259442"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0804558105", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011610376"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.neuron.2004.12.054", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011665747"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.95.11.6256", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013984237"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-3-642-88418-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017519325", 
          "https://doi.org/10.1007/978-3-642-88418-4"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-3-642-88418-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017519325", 
          "https://doi.org/10.1007/978-3-642-88418-4"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrm2009", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019849472", 
          "https://doi.org/10.1038/nrm2009"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrm2009", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019849472", 
          "https://doi.org/10.1038/nrm2009"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/cercor/bhr033", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020439343"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0896-6273(00)00028-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020772140"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/gb-2009-10-10-r104", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025460148", 
          "https://doi.org/10.1186/gb-2009-10-10-r104"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/dvdy.20079", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028116832"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.7554/elife.02196", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028656993"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35102174", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029692486", 
          "https://doi.org/10.1038/35102174"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35102174", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029692486", 
          "https://doi.org/10.1038/35102174"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0131266", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030970872"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.devcel.2007.05.014", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032617059"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1750-3639.2007.00113.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032809571"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3389/fncel.2014.00396", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035093834"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0896-6273(00)81171-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035874506"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ygeno.2007.08.005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037436343"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1523/jneurosci.3406-07.2007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038299068"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1471-2164-15-624", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042511165", 
          "https://doi.org/10.1186/1471-2164-15-624"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.expneurol.2006.02.005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042696109"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1750-3639.2010.00459.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043036081"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrg1448", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043277982", 
          "https://doi.org/10.1038/nrg1448"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrg1448", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043277982", 
          "https://doi.org/10.1038/nrg1448"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.91.11.4997", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043355865"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ncb960", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046703252", 
          "https://doi.org/10.1038/ncb960"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ncb960", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046703252", 
          "https://doi.org/10.1038/ncb960"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/hipo.20308", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051685660"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0896-6273(00)81172-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052646552"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/stem.1297", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053279484"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2144/05382rr05", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1069097011"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3748/wjg.v21.i3.878", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1071372818"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1176/ajp.156.4.564", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1074357393"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1523/jneurosci.20-16-06095.2000", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1074681748"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077615671", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083245898", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2019-04", 
    "datePublishedReg": "2019-04-01", 
    "description": "Notch signalling pathway is involved in the proliferation of neural progenitor cells (NPCs), to inhibit neuronal cell commitment and to promote glial cell fate. Notch protein is cleaved by gamma-secretase, a multisubunit transmembrane protein complex that releases the Notch intracellular domain (NICD) and subsequently activates the downstream targets. Down syndrome (DS) individuals exhibit an increased number of glial cells (particularly astrocytes), and reduced number of neurons suggesting the involvement of Notch signalling pathway in the neurogenic-to-gliogenic shift in DS brain. Ts1Cje is a DS mouse model that exhibit similar neuropathology to human DS individuals. To date, the spatiotemporal gene expression of the Notch and gamma-secretase genes have not been characterised in Ts1Cje mouse brain. Understanding the expression pattern of Notch and gamma-secretase genes may provide a better understanding of the underlying mechanism that leads to the shift. Gene expression analysis using RT-qPCR was performed on early embryonic and postnatal development of DS brain. In the developing mouse brain, mRNA expression analysis showed that gamma-secretase members (Psen1, Pen-2, Aph-1b, and Ncstn) were not differentially expressed. Notch2 was found to be downregulated in the developing Ts1Cje brain samples. Postnatal gene expression study showed complex expression patterns and Notch1 and Notch2 genes were found to be significantly downregulated in the hippocampus at postnatal day 30. Results from RT-qPCR analysis from E15.5 neurosphere culture showed an increase of expression of Psen1, and Aph-1b but downregulation of Pen-2 and Ncstn genes. Gamma-secretase activity in Ts1Cje E15.5 neurospheres was significantly increased by fivefold. In summary, the association and the role of Notch and gamma-secretase gene expression throughout development with neurogenic-to-gliogenic shift in Ts1Cje remain undefined and warrant further validation.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s12031-019-01275-2", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1094289", 
        "issn": [
          "0895-8696", 
          "2168-8729"
        ], 
        "name": "Journal of Molecular Neuroscience", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "4", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "67"
      }
    ], 
    "name": "Expression Profiling of Notch Signalling Pathway and Gamma-Secretase Activity in the Brain of Ts1Cje Mouse Model of Down Syndrome", 
    "pagination": "1-11", 
    "productId": [
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s12031-019-01275-2"
        ]
      }, 
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "0d2b86348eba03dd4e62ef63b17888ea503bdf8cd04e68e36ee602a773a1e661"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1112096426"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "9002991"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "30758748"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s12031-019-01275-2", 
      "https://app.dimensions.ai/details/publication/pub.1112096426"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-15T09:19", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000376_0000000376/records_56176_00000006.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://link.springer.com/10.1007%2Fs12031-019-01275-2"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s12031-019-01275-2'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s12031-019-01275-2'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s12031-019-01275-2'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s12031-019-01275-2'


 

This table displays all metadata directly associated to this object as RDF triples.

262 TRIPLES      21 PREDICATES      69 URIs      21 LITERALS      9 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s12031-019-01275-2 schema:about anzsrc-for:06
2 anzsrc-for:0604
3 schema:author N2eb1d1d02e5046b4938a133a9badaca4
4 schema:citation sg:pub.10.1007/978-3-642-88418-4
5 sg:pub.10.1038/35102174
6 sg:pub.10.1038/ncb960
7 sg:pub.10.1038/nrg1448
8 sg:pub.10.1038/nrm2009
9 sg:pub.10.1186/1471-2164-15-624
10 sg:pub.10.1186/gb-2009-10-10-r104
11 https://app.dimensions.ai/details/publication/pub.1077615671
12 https://app.dimensions.ai/details/publication/pub.1083245898
13 https://doi.org/10.1002/(sici)1097-4695(199912)41:4<524::aid-neu8>3.0.co;2-i
14 https://doi.org/10.1002/dvdy.20079
15 https://doi.org/10.1002/hipo.20308
16 https://doi.org/10.1002/stem.1297
17 https://doi.org/10.1016/j.devcel.2007.05.014
18 https://doi.org/10.1016/j.expneurol.2006.02.005
19 https://doi.org/10.1016/j.neuron.2004.12.054
20 https://doi.org/10.1016/j.ygeno.2007.08.005
21 https://doi.org/10.1016/s0896-6273(00)00028-3
22 https://doi.org/10.1016/s0896-6273(00)81171-x
23 https://doi.org/10.1016/s0896-6273(00)81172-1
24 https://doi.org/10.1073/pnas.0804558105
25 https://doi.org/10.1073/pnas.91.11.4997
26 https://doi.org/10.1073/pnas.95.11.6256
27 https://doi.org/10.1074/jbc.m111.300483
28 https://doi.org/10.1093/cercor/bhp176
29 https://doi.org/10.1093/cercor/bhr033
30 https://doi.org/10.1093/emboj/18.8.2196
31 https://doi.org/10.1111/j.1750-3639.2007.00113.x
32 https://doi.org/10.1111/j.1750-3639.2010.00459.x
33 https://doi.org/10.1176/ajp.156.4.564
34 https://doi.org/10.1242/dev.063610
35 https://doi.org/10.1371/journal.pone.0011561
36 https://doi.org/10.1371/journal.pone.0131266
37 https://doi.org/10.1523/jneurosci.20-16-06095.2000
38 https://doi.org/10.1523/jneurosci.3406-07.2007
39 https://doi.org/10.1523/jneurosci.4767-10.2011
40 https://doi.org/10.2144/05382rr05
41 https://doi.org/10.3389/fncel.2014.00396
42 https://doi.org/10.3748/wjg.v21.i3.878
43 https://doi.org/10.7554/elife.02196
44 schema:datePublished 2019-04
45 schema:datePublishedReg 2019-04-01
46 schema:description Notch signalling pathway is involved in the proliferation of neural progenitor cells (NPCs), to inhibit neuronal cell commitment and to promote glial cell fate. Notch protein is cleaved by gamma-secretase, a multisubunit transmembrane protein complex that releases the Notch intracellular domain (NICD) and subsequently activates the downstream targets. Down syndrome (DS) individuals exhibit an increased number of glial cells (particularly astrocytes), and reduced number of neurons suggesting the involvement of Notch signalling pathway in the neurogenic-to-gliogenic shift in DS brain. Ts1Cje is a DS mouse model that exhibit similar neuropathology to human DS individuals. To date, the spatiotemporal gene expression of the Notch and gamma-secretase genes have not been characterised in Ts1Cje mouse brain. Understanding the expression pattern of Notch and gamma-secretase genes may provide a better understanding of the underlying mechanism that leads to the shift. Gene expression analysis using RT-qPCR was performed on early embryonic and postnatal development of DS brain. In the developing mouse brain, mRNA expression analysis showed that gamma-secretase members (Psen1, Pen-2, Aph-1b, and Ncstn) were not differentially expressed. Notch2 was found to be downregulated in the developing Ts1Cje brain samples. Postnatal gene expression study showed complex expression patterns and Notch1 and Notch2 genes were found to be significantly downregulated in the hippocampus at postnatal day 30. Results from RT-qPCR analysis from E15.5 neurosphere culture showed an increase of expression of Psen1, and Aph-1b but downregulation of Pen-2 and Ncstn genes. Gamma-secretase activity in Ts1Cje E15.5 neurospheres was significantly increased by fivefold. In summary, the association and the role of Notch and gamma-secretase gene expression throughout development with neurogenic-to-gliogenic shift in Ts1Cje remain undefined and warrant further validation.
47 schema:genre research_article
48 schema:inLanguage en
49 schema:isAccessibleForFree false
50 schema:isPartOf N0a9b0adf847a4ccb8bbfa8c3f17f5543
51 N915225c00771439db74b62da29bbe168
52 sg:journal.1094289
53 schema:name Expression Profiling of Notch Signalling Pathway and Gamma-Secretase Activity in the Brain of Ts1Cje Mouse Model of Down Syndrome
54 schema:pagination 1-11
55 schema:productId N121dc522874a4f5ab766ea7f9692ad3c
56 N5175f1b62a404c05b4d0df7c62026d62
57 N70a609865d2d45afae7ca53d26543444
58 Nbac4f62e64564900aeb2638fb19f8c12
59 Nd3d6797f751847569ad94e873a21670c
60 schema:sameAs https://app.dimensions.ai/details/publication/pub.1112096426
61 https://doi.org/10.1007/s12031-019-01275-2
62 schema:sdDatePublished 2019-04-15T09:19
63 schema:sdLicense https://scigraph.springernature.com/explorer/license/
64 schema:sdPublisher Nfc4731ddbfa94f708494dfae4f83be7d
65 schema:url https://link.springer.com/10.1007%2Fs12031-019-01275-2
66 sgo:license sg:explorer/license/
67 sgo:sdDataset articles
68 rdf:type schema:ScholarlyArticle
69 N0a9b0adf847a4ccb8bbfa8c3f17f5543 schema:issueNumber 4
70 rdf:type schema:PublicationIssue
71 N0c67a351f4864070b608cb96b82b79b6 schema:affiliation https://www.grid.ac/institutes/grid.11142.37
72 schema:familyName Cheah
73 schema:givenName Pike-See
74 rdf:type schema:Person
75 N119a3e849fcf4dada2752bc8427e74b9 schema:affiliation https://www.grid.ac/institutes/grid.11142.37
76 schema:familyName Lee
77 schema:givenName Han-Chung
78 rdf:type schema:Person
79 N121dc522874a4f5ab766ea7f9692ad3c schema:name dimensions_id
80 schema:value pub.1112096426
81 rdf:type schema:PropertyValue
82 N172bf1cf4dac4612a5c76d1615554750 schema:affiliation https://www.grid.ac/institutes/grid.11142.37
83 schema:familyName Yusof
84 schema:givenName Hadri Hadi
85 rdf:type schema:Person
86 N1b8b3dcf4093458eb7571a35eb041bc7 schema:affiliation https://www.grid.ac/institutes/grid.51462.34
87 schema:familyName Li
88 schema:givenName Yue-Ming
89 rdf:type schema:Person
90 N217f555b27b648f498d8ceb988281927 rdf:first N6787d602b7324f469e7fb43406bd9520
91 rdf:rest Nd79235d2e23d40cf8c6469af66dd9e9c
92 N2eb1d1d02e5046b4938a133a9badaca4 rdf:first N172bf1cf4dac4612a5c76d1615554750
93 rdf:rest Nf753cd4885bc426a97f3d747d8740e5a
94 N451c833ad9214784a5ffaca0c38d264e schema:affiliation https://www.grid.ac/institutes/grid.51462.34
95 schema:familyName Wu
96 schema:givenName Xiangzhong
97 rdf:type schema:Person
98 N4f203394ce944109a8821cd8ac4da343 schema:affiliation https://www.grid.ac/institutes/grid.11142.37
99 schema:familyName Chau
100 schema:givenName De-Ming
101 rdf:type schema:Person
102 N5175f1b62a404c05b4d0df7c62026d62 schema:name readcube_id
103 schema:value 0d2b86348eba03dd4e62ef63b17888ea503bdf8cd04e68e36ee602a773a1e661
104 rdf:type schema:PropertyValue
105 N593563c9c2cb47b8b2bd7439639b8da3 schema:affiliation https://www.grid.ac/institutes/grid.11142.37
106 schema:familyName Ling
107 schema:givenName King-Hwa
108 rdf:type schema:Person
109 N6787d602b7324f469e7fb43406bd9520 schema:affiliation https://www.grid.ac/institutes/grid.470344.0
110 schema:familyName Scott
111 schema:givenName Hamish S
112 rdf:type schema:Person
113 N70a609865d2d45afae7ca53d26543444 schema:name pubmed_id
114 schema:value 30758748
115 rdf:type schema:PropertyValue
116 N8523afe42f3a46d8a4552a27fc95cf0b rdf:first N593563c9c2cb47b8b2bd7439639b8da3
117 rdf:rest rdf:nil
118 N915225c00771439db74b62da29bbe168 schema:volumeNumber 67
119 rdf:type schema:PublicationVolume
120 N99141e7c36984b8a94766d4335c30df0 schema:affiliation https://www.grid.ac/institutes/grid.11142.37
121 schema:familyName Seth
122 schema:givenName Eryse Amira
123 rdf:type schema:Person
124 Nb3b7fc4e265a4f05a576351f0c8a674c rdf:first N1b8b3dcf4093458eb7571a35eb041bc7
125 rdf:rest Nc8c0b3c1da614cdd955a8dd8e91e847c
126 Nbac4f62e64564900aeb2638fb19f8c12 schema:name doi
127 schema:value 10.1007/s12031-019-01275-2
128 rdf:type schema:PropertyValue
129 Nc7e3e3ec0ff349c0a9b248ae6808cbe6 schema:affiliation https://www.grid.ac/institutes/grid.1055.1
130 schema:familyName Hewitt
131 schema:givenName Chelsee A.
132 rdf:type schema:Person
133 Nc8c0b3c1da614cdd955a8dd8e91e847c rdf:first N4f203394ce944109a8821cd8ac4da343
134 rdf:rest N8523afe42f3a46d8a4552a27fc95cf0b
135 Nd3d6797f751847569ad94e873a21670c schema:name nlm_unique_id
136 schema:value 9002991
137 rdf:type schema:PropertyValue
138 Nd79235d2e23d40cf8c6469af66dd9e9c rdf:first N0c67a351f4864070b608cb96b82b79b6
139 rdf:rest Nb3b7fc4e265a4f05a576351f0c8a674c
140 Ne7fc7afa7afd481287b899b1c3a6c659 rdf:first Nc7e3e3ec0ff349c0a9b248ae6808cbe6
141 rdf:rest N217f555b27b648f498d8ceb988281927
142 Ne853fbeba5da4e2c89cc1f3d51593cb0 rdf:first N451c833ad9214784a5ffaca0c38d264e
143 rdf:rest Ne7fc7afa7afd481287b899b1c3a6c659
144 Nf753cd4885bc426a97f3d747d8740e5a rdf:first N119a3e849fcf4dada2752bc8427e74b9
145 rdf:rest Nfd2e6a81c3cf4f458b3ed2e2e6437d65
146 Nfc4731ddbfa94f708494dfae4f83be7d schema:name Springer Nature - SN SciGraph project
147 rdf:type schema:Organization
148 Nfd2e6a81c3cf4f458b3ed2e2e6437d65 rdf:first N99141e7c36984b8a94766d4335c30df0
149 rdf:rest Ne853fbeba5da4e2c89cc1f3d51593cb0
150 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
151 schema:name Biological Sciences
152 rdf:type schema:DefinedTerm
153 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
154 schema:name Genetics
155 rdf:type schema:DefinedTerm
156 sg:journal.1094289 schema:issn 0895-8696
157 2168-8729
158 schema:name Journal of Molecular Neuroscience
159 rdf:type schema:Periodical
160 sg:pub.10.1007/978-3-642-88418-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017519325
161 https://doi.org/10.1007/978-3-642-88418-4
162 rdf:type schema:CreativeWork
163 sg:pub.10.1038/35102174 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029692486
164 https://doi.org/10.1038/35102174
165 rdf:type schema:CreativeWork
166 sg:pub.10.1038/ncb960 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046703252
167 https://doi.org/10.1038/ncb960
168 rdf:type schema:CreativeWork
169 sg:pub.10.1038/nrg1448 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043277982
170 https://doi.org/10.1038/nrg1448
171 rdf:type schema:CreativeWork
172 sg:pub.10.1038/nrm2009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019849472
173 https://doi.org/10.1038/nrm2009
174 rdf:type schema:CreativeWork
175 sg:pub.10.1186/1471-2164-15-624 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042511165
176 https://doi.org/10.1186/1471-2164-15-624
177 rdf:type schema:CreativeWork
178 sg:pub.10.1186/gb-2009-10-10-r104 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025460148
179 https://doi.org/10.1186/gb-2009-10-10-r104
180 rdf:type schema:CreativeWork
181 https://app.dimensions.ai/details/publication/pub.1077615671 schema:CreativeWork
182 https://app.dimensions.ai/details/publication/pub.1083245898 schema:CreativeWork
183 https://doi.org/10.1002/(sici)1097-4695(199912)41:4<524::aid-neu8>3.0.co;2-i schema:sameAs https://app.dimensions.ai/details/publication/pub.1007500182
184 rdf:type schema:CreativeWork
185 https://doi.org/10.1002/dvdy.20079 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028116832
186 rdf:type schema:CreativeWork
187 https://doi.org/10.1002/hipo.20308 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051685660
188 rdf:type schema:CreativeWork
189 https://doi.org/10.1002/stem.1297 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053279484
190 rdf:type schema:CreativeWork
191 https://doi.org/10.1016/j.devcel.2007.05.014 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032617059
192 rdf:type schema:CreativeWork
193 https://doi.org/10.1016/j.expneurol.2006.02.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042696109
194 rdf:type schema:CreativeWork
195 https://doi.org/10.1016/j.neuron.2004.12.054 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011665747
196 rdf:type schema:CreativeWork
197 https://doi.org/10.1016/j.ygeno.2007.08.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037436343
198 rdf:type schema:CreativeWork
199 https://doi.org/10.1016/s0896-6273(00)00028-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020772140
200 rdf:type schema:CreativeWork
201 https://doi.org/10.1016/s0896-6273(00)81171-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1035874506
202 rdf:type schema:CreativeWork
203 https://doi.org/10.1016/s0896-6273(00)81172-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052646552
204 rdf:type schema:CreativeWork
205 https://doi.org/10.1073/pnas.0804558105 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011610376
206 rdf:type schema:CreativeWork
207 https://doi.org/10.1073/pnas.91.11.4997 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043355865
208 rdf:type schema:CreativeWork
209 https://doi.org/10.1073/pnas.95.11.6256 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013984237
210 rdf:type schema:CreativeWork
211 https://doi.org/10.1074/jbc.m111.300483 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011259442
212 rdf:type schema:CreativeWork
213 https://doi.org/10.1093/cercor/bhp176 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010057857
214 rdf:type schema:CreativeWork
215 https://doi.org/10.1093/cercor/bhr033 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020439343
216 rdf:type schema:CreativeWork
217 https://doi.org/10.1093/emboj/18.8.2196 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000982033
218 rdf:type schema:CreativeWork
219 https://doi.org/10.1111/j.1750-3639.2007.00113.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1032809571
220 rdf:type schema:CreativeWork
221 https://doi.org/10.1111/j.1750-3639.2010.00459.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1043036081
222 rdf:type schema:CreativeWork
223 https://doi.org/10.1176/ajp.156.4.564 schema:sameAs https://app.dimensions.ai/details/publication/pub.1074357393
224 rdf:type schema:CreativeWork
225 https://doi.org/10.1242/dev.063610 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010560245
226 rdf:type schema:CreativeWork
227 https://doi.org/10.1371/journal.pone.0011561 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010538191
228 rdf:type schema:CreativeWork
229 https://doi.org/10.1371/journal.pone.0131266 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030970872
230 rdf:type schema:CreativeWork
231 https://doi.org/10.1523/jneurosci.20-16-06095.2000 schema:sameAs https://app.dimensions.ai/details/publication/pub.1074681748
232 rdf:type schema:CreativeWork
233 https://doi.org/10.1523/jneurosci.3406-07.2007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038299068
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1523/jneurosci.4767-10.2011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001743661
236 rdf:type schema:CreativeWork
237 https://doi.org/10.2144/05382rr05 schema:sameAs https://app.dimensions.ai/details/publication/pub.1069097011
238 rdf:type schema:CreativeWork
239 https://doi.org/10.3389/fncel.2014.00396 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035093834
240 rdf:type schema:CreativeWork
241 https://doi.org/10.3748/wjg.v21.i3.878 schema:sameAs https://app.dimensions.ai/details/publication/pub.1071372818
242 rdf:type schema:CreativeWork
243 https://doi.org/10.7554/elife.02196 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028656993
244 rdf:type schema:CreativeWork
245 https://www.grid.ac/institutes/grid.1055.1 schema:alternateName Peter MacCallum Cancer Centre
246 schema:name Department of Pathology, The Peter MacCallum Cancer Centre, East Melbourne, Victoria, Australia
247 rdf:type schema:Organization
248 https://www.grid.ac/institutes/grid.11142.37 schema:alternateName Universiti Putra Malaysia
249 schema:name Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia
250 Department of Human Anatomy, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia
251 Genetics & Regenerative Medicine Research Centre (GRMRC), Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia
252 rdf:type schema:Organization
253 https://www.grid.ac/institutes/grid.470344.0 schema:alternateName Centre for Cancer Biology
254 schema:name Australian Cancer Research Foundation Genomics Facility, Centre for Cancer Biology, SA Pathology, Adelaide, Australia
255 Department of Genetics and Molecular Pathology, Centre for Cancer Biology, An Alliance Between SA Pathology and the University of South Australia, SA Pathology, Adelaide, Australia
256 School of Biological Sciences, University of Adelaide, Adelaide, Australia
257 School of Medicine, Faculty of Health Sciences, University of Adelaide, Adelaide, SA, Australia
258 School of Pharmacy and Medical Science, University of South Australia, Adelaide, Australia
259 rdf:type schema:Organization
260 https://www.grid.ac/institutes/grid.51462.34 schema:alternateName Memorial Sloan Kettering Cancer Center
261 schema:name Chemical Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY, USA
262 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...