sg:pub.10.1007/s12030-009-9029-1 - Springer Nature SciGraph

Article Info

DATE

2009-09-16

AUTHORS

Nilda Vanesa Ayala-Núñez, Humberto H. Lara Villegas, Liliana del Carmen Ixtepan Turrent, Cristina Rodríguez Padilla

ABSTRACT

Silver nanoparticles, which are being used increasingly as antimicrobial agents, may extend its antibacterial application to methicillin-resistant Staphylococcus aureus (MRSA), the main cause of nosocomial infections worldwide. To explore the antibacterial properties of silver nanoparticles against MRSA, the present work includes an analysis of the relation between nanosilver effect and MRSA’s resistance mechanisms, a study of the size dependence of the bactericidal activity of nanosilver and a toxicity assessment of nanoparticles against epithelial human cells. Minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and MBC/MIC ratio of silver nanoparticles were quantified by using a luciferase-based assay. The cytotoxic effect (CC50 and CC90) of three different nanosilver sizes (10, 30–40, and 100 nm) were assessed in HeLa cells by a similar method. The therapeutic index was used as an indicator of nanosilver overall efficacy and safety. Silver nanoparticles inhibited bacterial growth of both MRSA and non-MR S. aureus in a bactericidal rather than a bacteriostatic manner (MBC/MIC ratio ≤ 4). Silver nanoparticle’s therapeutic index varied when nanoparticle’s size diminished. At the same dose range, 10 nm nanoparticles were the most effective since they did not affect HeLa’s cell viability while inhibiting a considerable percentage of MRSA growth. Silver nanoparticles are effective bactericidal agents that are not affected by drug-resistant mechanisms of MRSA. Nanosilver size mediates MRSA inhibition and the cytotoxicity to human cells, being smaller nanoparticles the ones with a better antibacterial activity and nontoxic effect. More... »

PAGES

2-9

References to SciGraph publications

2005-06-29. Interaction of silver nanoparticles with HIV-1 in JOURNAL OF NANOBIOTECHNOLOGY

2007. Clinical, Epidemiological, and Laboratory Aspects of Methicillin-Resistant Staphylococcus aureus (MRSA) Infections in METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) PROTOCOLS

2007-11-12. Inhibitory activity spectrum of reuterin produced by Lactobacillus reuteri against intestinal bacteria in BMC MICROBIOLOGY

Journal

TITLE

NanoBiotechnology

ISSUE

1-4

VOLUME

Subjects

FOR: Technology

FOR: Nanotechnology

Author Affiliations

Immunology and Virology Laboratory, Universidad Autonoma de Nuevo Leon, Edificio C, 3er piso, Facultad de Ciencias Biologicas, Ave. Pedro de Alba S/N, Ciudad Universitaria, C.P. 66451, San Nicolas de los Garza, Nuevo Leon, Mexico

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s12030-009-9029-1

DOI

http://dx.doi.org/10.1007/s12030-009-9029-1

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1020569270

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