Lambda phage nanoparticles displaying HER2-derived E75 peptide induce effective E75-CD8+ T response View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2017-11-16

AUTHORS

Atefeh Arab, Jessica Nicastro, Roderick Slavcev, Atefeh Razazan, Nastaran Barati, Amin Reza Nikpoor, Amir Abbas Momtazi Brojeni, Fatemeh Mosaffa, Ali Badiee, Mahmoud Reza Jaafari, Javad Behravan

ABSTRACT

We have investigated the in vitro immunogenicity and in vivo prophylactic and therapeutic potential of lambda (λ) phage particles displaying the E75 peptide (derived from HER2 protein) in an implantable TUBO breast tumor model of BALB/c mice. The mice were immunized with the E75-displaying phage (λF7-gpD::E75) every 2-week intervals over a 6-week period, and the generated immune responses were studied. Results showed in vitro induction of immune responses by the λF7 (gpD::E75) construct compared to the control λF7 and buffer groups. In the in vivo prophylactic study, all the control and vaccinated mice groups developed tumors. However, in the therapeutic experiments, we observed a significant difference in tumor size at days 14–36 for mice immunized with λF7 (gpD::E75) compared to control groups (P < 0.05). Moreover, the survival time prolonged in mice immunized with λF7 (gpD::E75). The discrepancy between the results obtained from the in vitro and in vivo studies may have been a result of the induction of Foxp3 CD4+CD25+ which has been previously reported to hamper effective T cell functionality. In conclusion, we observed a significant immune stimulatory response in the in vitro study, while in vivo, the vaccine was not able to exert significant tumor inhibitory effects. We suggest that the presence of Foxp3+ CD4+CD25+ cells may have impaired the anti-tumor response in mice challenged in vivo with the TUBO xenograft tumor. More... »

PAGES

200-206

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s12026-017-8969-0

DOI

http://dx.doi.org/10.1007/s12026-017-8969-0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1092710770

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/29143917


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1107", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Immunology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Bacteriophage lambda", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Breast Neoplasms", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "CD8-Positive T-Lymphocytes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cells, Cultured", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Disease Models, Animal", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Epitopes, T-Lymphocyte", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Female", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Forkhead Transcription Factors", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Glucosephosphate Dehydrogenase", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice, Inbred BALB C", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Nanoparticles", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Peptide Fragments", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Receptor, ErbB-2", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "T-Lymphocytes, Regulatory", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Tumor Burden", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Vaccination", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran", 
          "id": "http://www.grid.ac/institutes/grid.411583.a", 
          "name": [
            "Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Arab", 
        "givenName": "Atefeh", 
        "id": "sg:person.0777504475.25", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0777504475.25"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Waterloo Institute of Nanotechnology, University of Waterloo, 200 University Ave, N2L3G1, Waterloo, ON, Canada", 
          "id": "http://www.grid.ac/institutes/grid.46078.3d", 
          "name": [
            "School of Pharmacy, University of Waterloo, 200 University Ave, N2L3G1, Waterloo, ON, Canada", 
            "Waterloo Institute of Nanotechnology, University of Waterloo, 200 University Ave, N2L3G1, Waterloo, ON, Canada"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nicastro", 
        "givenName": "Jessica", 
        "id": "sg:person.012035134537.50", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012035134537.50"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Mediphage Bioceuticals, Inc., 661 University Avenue, Suite 1300, MaRS Centre, West Tower, M5G0B7, Toronto, ON, Canada", 
          "id": "http://www.grid.ac/institutes/grid.498726.6", 
          "name": [
            "School of Pharmacy, University of Waterloo, 200 University Ave, N2L3G1, Waterloo, ON, Canada", 
            "Waterloo Institute of Nanotechnology, University of Waterloo, 200 University Ave, N2L3G1, Waterloo, ON, Canada", 
            "Mediphage Bioceuticals, Inc., 661 University Avenue, Suite 1300, MaRS Centre, West Tower, M5G0B7, Toronto, ON, Canada"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Slavcev", 
        "givenName": "Roderick", 
        "id": "sg:person.01140151626.62", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01140151626.62"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran", 
          "id": "http://www.grid.ac/institutes/grid.411705.6", 
          "name": [
            "Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Razazan", 
        "givenName": "Atefeh", 
        "id": "sg:person.015330433223.05", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015330433223.05"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran", 
          "id": "http://www.grid.ac/institutes/grid.411583.a", 
          "name": [
            "Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Barati", 
        "givenName": "Nastaran", 
        "id": "sg:person.014247646065.54", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014247646065.54"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Immunology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran", 
          "id": "http://www.grid.ac/institutes/grid.411583.a", 
          "name": [
            "Department of Immunology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nikpoor", 
        "givenName": "Amin Reza", 
        "id": "sg:person.01176431351.61", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01176431351.61"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Student Research Committee, Mashhad University of Medical Sciences, Mashhad, Iran", 
          "id": "http://www.grid.ac/institutes/grid.411583.a", 
          "name": [
            "Student Research Committee, Mashhad University of Medical Sciences, Mashhad, Iran"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Brojeni", 
        "givenName": "Amir Abbas Momtazi", 
        "id": "sg:person.013273113567.29", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013273113567.29"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran", 
          "id": "http://www.grid.ac/institutes/grid.411583.a", 
          "name": [
            "Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Mosaffa", 
        "givenName": "Fatemeh", 
        "id": "sg:person.01063747464.88", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01063747464.88"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran", 
          "id": "http://www.grid.ac/institutes/grid.411583.a", 
          "name": [
            "Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Badiee", 
        "givenName": "Ali", 
        "id": "sg:person.0737230244.43", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0737230244.43"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran", 
          "id": "http://www.grid.ac/institutes/grid.411583.a", 
          "name": [
            "Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Jaafari", 
        "givenName": "Mahmoud Reza", 
        "id": "sg:person.01236040670.30", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01236040670.30"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "School of Pharmacy, University of Waterloo, 200 University Ave, N2L3G1, Waterloo, ON, Canada", 
          "id": "http://www.grid.ac/institutes/grid.46078.3d", 
          "name": [
            "Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran", 
            "School of Pharmacy, University of Waterloo, 200 University Ave, N2L3G1, Waterloo, ON, Canada"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Behravan", 
        "givenName": "Javad", 
        "id": "sg:person.01245756105.18", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01245756105.18"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/78490", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052632693", 
          "https://doi.org/10.1038/78490"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrc1586", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020273083", 
          "https://doi.org/10.1038/nrc1586"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ni904", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053106484", 
          "https://doi.org/10.1038/ni904"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ni1178", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034654370", 
          "https://doi.org/10.1038/ni1178"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00262-008-0540-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015040063", 
          "https://doi.org/10.1007/s00262-008-0540-3"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature08604", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012248564", 
          "https://doi.org/10.1038/nature08604"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nm0698-705", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026188602", 
          "https://doi.org/10.1038/nm0698-705"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-013-4898-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039546839", 
          "https://doi.org/10.1007/s00253-013-4898-6"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nm934", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015972513", 
          "https://doi.org/10.1038/nm934"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2017-11-16", 
    "datePublishedReg": "2017-11-16", 
    "description": "We have investigated the in vitro immunogenicity and in vivo prophylactic and therapeutic potential of lambda (\u03bb) phage particles displaying the E75 peptide (derived from HER2 protein) in an implantable TUBO breast tumor model of BALB/c mice. The mice were immunized with the E75-displaying phage (\u03bbF7-gpD::E75) every 2-week intervals over a 6-week period, and the generated immune responses were studied. Results showed in vitro induction of immune responses by the \u03bbF7 (gpD::E75) construct compared to the control \u03bbF7 and buffer groups. In the in vivo prophylactic study, all the control and vaccinated mice groups developed tumors. However, in the therapeutic experiments, we observed a significant difference in tumor size at days 14\u201336 for mice immunized with \u03bbF7 (gpD::E75) compared to control groups (P\u2009<\u20090.05). Moreover, the survival time prolonged in mice immunized with \u03bbF7 (gpD::E75). The discrepancy between the results obtained from the in vitro and in vivo studies may have been a result of the induction of Foxp3 CD4+CD25+ which has been previously reported to hamper effective T cell functionality. In conclusion, we observed a significant immune stimulatory response in the in vitro study, while in vivo, the vaccine was not able to exert significant tumor inhibitory effects. We suggest that the presence of Foxp3+ CD4+CD25+ cells may have impaired the anti-tumor response in mice challenged in vivo with the TUBO xenograft tumor.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/s12026-017-8969-0", 
    "inLanguage": "en", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1096613", 
        "issn": [
          "0257-277X", 
          "1559-0755"
        ], 
        "name": "Immunologic Research", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "66"
      }
    ], 
    "keywords": [
      "E75 peptide", 
      "immune response", 
      "anti-tumor responses", 
      "BALB/c mice", 
      "presence of Foxp3", 
      "induction of Foxp3", 
      "tumor inhibitory effect", 
      "significant tumor inhibitory effects", 
      "tumor size", 
      "mice groups", 
      "breast tumor model", 
      "prophylactic studies", 
      "survival time", 
      "therapeutic experiments", 
      "xenograft tumors", 
      "day 14", 
      "tumor model", 
      "therapeutic potential", 
      "stimulatory response", 
      "inhibitory effect", 
      "mice", 
      "vivo studies", 
      "phage nanoparticles", 
      "Foxp3", 
      "significant differences", 
      "tumors", 
      "vivo", 
      "cell functionality", 
      "group", 
      "response", 
      "induction", 
      "vaccine", 
      "immunogenicity", 
      "peptides", 
      "study", 
      "conclusion", 
      "cells", 
      "intervals", 
      "period", 
      "control", 
      "differences", 
      "lambda phage particles", 
      "results", 
      "phage particles", 
      "effect", 
      "presence", 
      "time", 
      "discrepancy", 
      "potential", 
      "phages", 
      "size", 
      "model", 
      "experiments", 
      "functionality", 
      "particles", 
      "nanoparticles", 
      "c mice", 
      "E75-displaying phage", 
      "\u03bbF7", 
      "control \u03bbF7", 
      "vivo prophylactic study", 
      "vaccinated mice groups", 
      "significant immune stimulatory response", 
      "immune stimulatory response", 
      "TUBO xenograft tumor", 
      "Lambda phage nanoparticles", 
      "effective E75-CD8", 
      "E75-CD8"
    ], 
    "name": "Lambda phage nanoparticles displaying HER2-derived E75 peptide induce effective E75-CD8+ T response", 
    "pagination": "200-206", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1092710770"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s12026-017-8969-0"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "29143917"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s12026-017-8969-0", 
      "https://app.dimensions.ai/details/publication/pub.1092710770"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2021-12-01T19:39", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20211201/entities/gbq_results/article/article_733.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/s12026-017-8969-0"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s12026-017-8969-0'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s12026-017-8969-0'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s12026-017-8969-0'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s12026-017-8969-0'


 

This table displays all metadata directly associated to this object as RDF triples.

332 TRIPLES      22 PREDICATES      122 URIs      105 LITERALS      26 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s12026-017-8969-0 schema:about N0a5adc3d81e74cc6a092f79d5a94582b
2 N0e068aa8c99c4cde997f2424f7a4639b
3 N179ba00b9ac84fd79a37f9bb707e1deb
4 N1dc1d123ff3d4339a1346109d92df1f6
5 N2e07f772c5204cf5a78afff44609a35e
6 N374b29a4098e4a70acad18c4a6cc2c0a
7 N5cf217c0fa484417a53d5672c4158416
8 N7637e29344d645adac32d9861205f736
9 N7c4420c1f17e4a6abc6e7850cb118f62
10 N82f92fa1d41b45fdba0907a1fc6c381c
11 N8c32d635489d4fc7827e5e428f555b54
12 Na3621e8b2c5246ebaa85e433ca809fe4
13 Na72b13996a504bfb9e5738953d9a892f
14 Nb3e24e242209442cb46059707f887293
15 Nc20e8b7ab6414e3799dae032b6876f9c
16 Nc820d2c6230c486ba1a0c7536c2afed8
17 Nda25b883f01c419f9d0a49679709a036
18 Ndf3d9e6cc0904eafa5dfc96f4c534a7a
19 Ne85e3591fc554cbf8e89a4bca466940f
20 anzsrc-for:11
21 anzsrc-for:1107
22 schema:author N025398e2cd0e4afdb15fef3e9b6211c3
23 schema:citation sg:pub.10.1007/s00253-013-4898-6
24 sg:pub.10.1007/s00262-008-0540-3
25 sg:pub.10.1038/78490
26 sg:pub.10.1038/nature08604
27 sg:pub.10.1038/ni1178
28 sg:pub.10.1038/ni904
29 sg:pub.10.1038/nm0698-705
30 sg:pub.10.1038/nm934
31 sg:pub.10.1038/nrc1586
32 schema:datePublished 2017-11-16
33 schema:datePublishedReg 2017-11-16
34 schema:description We have investigated the in vitro immunogenicity and in vivo prophylactic and therapeutic potential of lambda (λ) phage particles displaying the E75 peptide (derived from HER2 protein) in an implantable TUBO breast tumor model of BALB/c mice. The mice were immunized with the E75-displaying phage (λF7-gpD::E75) every 2-week intervals over a 6-week period, and the generated immune responses were studied. Results showed in vitro induction of immune responses by the λF7 (gpD::E75) construct compared to the control λF7 and buffer groups. In the in vivo prophylactic study, all the control and vaccinated mice groups developed tumors. However, in the therapeutic experiments, we observed a significant difference in tumor size at days 14–36 for mice immunized with λF7 (gpD::E75) compared to control groups (P < 0.05). Moreover, the survival time prolonged in mice immunized with λF7 (gpD::E75). The discrepancy between the results obtained from the in vitro and in vivo studies may have been a result of the induction of Foxp3 CD4+CD25+ which has been previously reported to hamper effective T cell functionality. In conclusion, we observed a significant immune stimulatory response in the in vitro study, while in vivo, the vaccine was not able to exert significant tumor inhibitory effects. We suggest that the presence of Foxp3+ CD4+CD25+ cells may have impaired the anti-tumor response in mice challenged in vivo with the TUBO xenograft tumor.
35 schema:genre article
36 schema:inLanguage en
37 schema:isAccessibleForFree false
38 schema:isPartOf N1429f5f6a8f345f0859dc7764f2c63c7
39 N945c40c67f5a4b8ba3d6aefba3dfca0c
40 sg:journal.1096613
41 schema:keywords BALB/c mice
42 E75 peptide
43 E75-CD8
44 E75-displaying phage
45 Foxp3
46 Lambda phage nanoparticles
47 TUBO xenograft tumor
48 anti-tumor responses
49 breast tumor model
50 c mice
51 cell functionality
52 cells
53 conclusion
54 control
55 control λF7
56 day 14
57 differences
58 discrepancy
59 effect
60 effective E75-CD8
61 experiments
62 functionality
63 group
64 immune response
65 immune stimulatory response
66 immunogenicity
67 induction
68 induction of Foxp3
69 inhibitory effect
70 intervals
71 lambda phage particles
72 mice
73 mice groups
74 model
75 nanoparticles
76 particles
77 peptides
78 period
79 phage nanoparticles
80 phage particles
81 phages
82 potential
83 presence
84 presence of Foxp3
85 prophylactic studies
86 response
87 results
88 significant differences
89 significant immune stimulatory response
90 significant tumor inhibitory effects
91 size
92 stimulatory response
93 study
94 survival time
95 therapeutic experiments
96 therapeutic potential
97 time
98 tumor inhibitory effect
99 tumor model
100 tumor size
101 tumors
102 vaccinated mice groups
103 vaccine
104 vivo
105 vivo prophylactic study
106 vivo studies
107 xenograft tumors
108 λF7
109 schema:name Lambda phage nanoparticles displaying HER2-derived E75 peptide induce effective E75-CD8+ T response
110 schema:pagination 200-206
111 schema:productId N68e9a877963948ec8d55b624426b1e09
112 Nbb94ba0f1b5b4331bc023ec4dc541b17
113 Ndab2718248b041878814fa260bab83d8
114 schema:sameAs https://app.dimensions.ai/details/publication/pub.1092710770
115 https://doi.org/10.1007/s12026-017-8969-0
116 schema:sdDatePublished 2021-12-01T19:39
117 schema:sdLicense https://scigraph.springernature.com/explorer/license/
118 schema:sdPublisher N186f65e8978e43d9b5039ad1fe92875b
119 schema:url https://doi.org/10.1007/s12026-017-8969-0
120 sgo:license sg:explorer/license/
121 sgo:sdDataset articles
122 rdf:type schema:ScholarlyArticle
123 N025398e2cd0e4afdb15fef3e9b6211c3 rdf:first sg:person.0777504475.25
124 rdf:rest N1f1153b40ae34816955d1e9cef7d80d0
125 N0a5adc3d81e74cc6a092f79d5a94582b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
126 schema:name Tumor Burden
127 rdf:type schema:DefinedTerm
128 N0e068aa8c99c4cde997f2424f7a4639b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
129 schema:name Forkhead Transcription Factors
130 rdf:type schema:DefinedTerm
131 N1429f5f6a8f345f0859dc7764f2c63c7 schema:issueNumber 1
132 rdf:type schema:PublicationIssue
133 N179ba00b9ac84fd79a37f9bb707e1deb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
134 schema:name Epitopes, T-Lymphocyte
135 rdf:type schema:DefinedTerm
136 N186f65e8978e43d9b5039ad1fe92875b schema:name Springer Nature - SN SciGraph project
137 rdf:type schema:Organization
138 N1dc1d123ff3d4339a1346109d92df1f6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
139 schema:name Disease Models, Animal
140 rdf:type schema:DefinedTerm
141 N1f1153b40ae34816955d1e9cef7d80d0 rdf:first sg:person.012035134537.50
142 rdf:rest Na77dbfbabad44039bd0cc98f0deab833
143 N2e07f772c5204cf5a78afff44609a35e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
144 schema:name Nanoparticles
145 rdf:type schema:DefinedTerm
146 N374b29a4098e4a70acad18c4a6cc2c0a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
147 schema:name Mice, Inbred BALB C
148 rdf:type schema:DefinedTerm
149 N5b692420a5c449ffb6ab5f6d626ac975 rdf:first sg:person.01176431351.61
150 rdf:rest N6cf069a449d5494c88b86a5f925ae6da
151 N5c205250c96741eea4752044854dfb30 rdf:first sg:person.01245756105.18
152 rdf:rest rdf:nil
153 N5cf217c0fa484417a53d5672c4158416 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
154 schema:name Glucosephosphate Dehydrogenase
155 rdf:type schema:DefinedTerm
156 N68e9a877963948ec8d55b624426b1e09 schema:name dimensions_id
157 schema:value pub.1092710770
158 rdf:type schema:PropertyValue
159 N6cf069a449d5494c88b86a5f925ae6da rdf:first sg:person.013273113567.29
160 rdf:rest Nef2eedd7ffea40f3972cb974a268a175
161 N7637e29344d645adac32d9861205f736 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
162 schema:name Peptide Fragments
163 rdf:type schema:DefinedTerm
164 N7c4420c1f17e4a6abc6e7850cb118f62 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
165 schema:name Bacteriophage lambda
166 rdf:type schema:DefinedTerm
167 N82f92fa1d41b45fdba0907a1fc6c381c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
168 schema:name Mice
169 rdf:type schema:DefinedTerm
170 N8b69ead8c3124a578e7a4df51899236b rdf:first sg:person.01236040670.30
171 rdf:rest N5c205250c96741eea4752044854dfb30
172 N8c32d635489d4fc7827e5e428f555b54 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
173 schema:name Humans
174 rdf:type schema:DefinedTerm
175 N945c40c67f5a4b8ba3d6aefba3dfca0c schema:volumeNumber 66
176 rdf:type schema:PublicationVolume
177 Na3621e8b2c5246ebaa85e433ca809fe4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
178 schema:name Receptor, ErbB-2
179 rdf:type schema:DefinedTerm
180 Na72b13996a504bfb9e5738953d9a892f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
181 schema:name Animals
182 rdf:type schema:DefinedTerm
183 Na77dbfbabad44039bd0cc98f0deab833 rdf:first sg:person.01140151626.62
184 rdf:rest Nae7c434a02ce4e97ae06f7dd529d9c28
185 Nae7c434a02ce4e97ae06f7dd529d9c28 rdf:first sg:person.015330433223.05
186 rdf:rest Nb82e69ab9d524534bc7e29cb0cf0b3d6
187 Nb3e24e242209442cb46059707f887293 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
188 schema:name CD8-Positive T-Lymphocytes
189 rdf:type schema:DefinedTerm
190 Nb82e69ab9d524534bc7e29cb0cf0b3d6 rdf:first sg:person.014247646065.54
191 rdf:rest N5b692420a5c449ffb6ab5f6d626ac975
192 Nbb94ba0f1b5b4331bc023ec4dc541b17 schema:name pubmed_id
193 schema:value 29143917
194 rdf:type schema:PropertyValue
195 Nc20e8b7ab6414e3799dae032b6876f9c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
196 schema:name Cells, Cultured
197 rdf:type schema:DefinedTerm
198 Nc820d2c6230c486ba1a0c7536c2afed8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
199 schema:name Breast Neoplasms
200 rdf:type schema:DefinedTerm
201 Nda25b883f01c419f9d0a49679709a036 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
202 schema:name Female
203 rdf:type schema:DefinedTerm
204 Ndab2718248b041878814fa260bab83d8 schema:name doi
205 schema:value 10.1007/s12026-017-8969-0
206 rdf:type schema:PropertyValue
207 Ndce42cb20d81466783aecbaa0974a483 rdf:first sg:person.0737230244.43
208 rdf:rest N8b69ead8c3124a578e7a4df51899236b
209 Ndf3d9e6cc0904eafa5dfc96f4c534a7a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
210 schema:name T-Lymphocytes, Regulatory
211 rdf:type schema:DefinedTerm
212 Ne85e3591fc554cbf8e89a4bca466940f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
213 schema:name Vaccination
214 rdf:type schema:DefinedTerm
215 Nef2eedd7ffea40f3972cb974a268a175 rdf:first sg:person.01063747464.88
216 rdf:rest Ndce42cb20d81466783aecbaa0974a483
217 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
218 schema:name Medical and Health Sciences
219 rdf:type schema:DefinedTerm
220 anzsrc-for:1107 schema:inDefinedTermSet anzsrc-for:
221 schema:name Immunology
222 rdf:type schema:DefinedTerm
223 sg:journal.1096613 schema:issn 0257-277X
224 1559-0755
225 schema:name Immunologic Research
226 schema:publisher Springer Nature
227 rdf:type schema:Periodical
228 sg:person.01063747464.88 schema:affiliation grid-institutes:grid.411583.a
229 schema:familyName Mosaffa
230 schema:givenName Fatemeh
231 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01063747464.88
232 rdf:type schema:Person
233 sg:person.01140151626.62 schema:affiliation grid-institutes:grid.498726.6
234 schema:familyName Slavcev
235 schema:givenName Roderick
236 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01140151626.62
237 rdf:type schema:Person
238 sg:person.01176431351.61 schema:affiliation grid-institutes:grid.411583.a
239 schema:familyName Nikpoor
240 schema:givenName Amin Reza
241 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01176431351.61
242 rdf:type schema:Person
243 sg:person.012035134537.50 schema:affiliation grid-institutes:grid.46078.3d
244 schema:familyName Nicastro
245 schema:givenName Jessica
246 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012035134537.50
247 rdf:type schema:Person
248 sg:person.01236040670.30 schema:affiliation grid-institutes:grid.411583.a
249 schema:familyName Jaafari
250 schema:givenName Mahmoud Reza
251 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01236040670.30
252 rdf:type schema:Person
253 sg:person.01245756105.18 schema:affiliation grid-institutes:grid.46078.3d
254 schema:familyName Behravan
255 schema:givenName Javad
256 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01245756105.18
257 rdf:type schema:Person
258 sg:person.013273113567.29 schema:affiliation grid-institutes:grid.411583.a
259 schema:familyName Brojeni
260 schema:givenName Amir Abbas Momtazi
261 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013273113567.29
262 rdf:type schema:Person
263 sg:person.014247646065.54 schema:affiliation grid-institutes:grid.411583.a
264 schema:familyName Barati
265 schema:givenName Nastaran
266 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014247646065.54
267 rdf:type schema:Person
268 sg:person.015330433223.05 schema:affiliation grid-institutes:grid.411705.6
269 schema:familyName Razazan
270 schema:givenName Atefeh
271 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015330433223.05
272 rdf:type schema:Person
273 sg:person.0737230244.43 schema:affiliation grid-institutes:grid.411583.a
274 schema:familyName Badiee
275 schema:givenName Ali
276 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0737230244.43
277 rdf:type schema:Person
278 sg:person.0777504475.25 schema:affiliation grid-institutes:grid.411583.a
279 schema:familyName Arab
280 schema:givenName Atefeh
281 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0777504475.25
282 rdf:type schema:Person
283 sg:pub.10.1007/s00253-013-4898-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039546839
284 https://doi.org/10.1007/s00253-013-4898-6
285 rdf:type schema:CreativeWork
286 sg:pub.10.1007/s00262-008-0540-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015040063
287 https://doi.org/10.1007/s00262-008-0540-3
288 rdf:type schema:CreativeWork
289 sg:pub.10.1038/78490 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052632693
290 https://doi.org/10.1038/78490
291 rdf:type schema:CreativeWork
292 sg:pub.10.1038/nature08604 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012248564
293 https://doi.org/10.1038/nature08604
294 rdf:type schema:CreativeWork
295 sg:pub.10.1038/ni1178 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034654370
296 https://doi.org/10.1038/ni1178
297 rdf:type schema:CreativeWork
298 sg:pub.10.1038/ni904 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053106484
299 https://doi.org/10.1038/ni904
300 rdf:type schema:CreativeWork
301 sg:pub.10.1038/nm0698-705 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026188602
302 https://doi.org/10.1038/nm0698-705
303 rdf:type schema:CreativeWork
304 sg:pub.10.1038/nm934 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015972513
305 https://doi.org/10.1038/nm934
306 rdf:type schema:CreativeWork
307 sg:pub.10.1038/nrc1586 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020273083
308 https://doi.org/10.1038/nrc1586
309 rdf:type schema:CreativeWork
310 grid-institutes:grid.411583.a schema:alternateName Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
311 Department of Immunology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
312 Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
313 Student Research Committee, Mashhad University of Medical Sciences, Mashhad, Iran
314 schema:name Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
315 Department of Immunology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
316 Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
317 Student Research Committee, Mashhad University of Medical Sciences, Mashhad, Iran
318 rdf:type schema:Organization
319 grid-institutes:grid.411705.6 schema:alternateName Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
320 schema:name Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
321 rdf:type schema:Organization
322 grid-institutes:grid.46078.3d schema:alternateName School of Pharmacy, University of Waterloo, 200 University Ave, N2L3G1, Waterloo, ON, Canada
323 Waterloo Institute of Nanotechnology, University of Waterloo, 200 University Ave, N2L3G1, Waterloo, ON, Canada
324 schema:name Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
325 School of Pharmacy, University of Waterloo, 200 University Ave, N2L3G1, Waterloo, ON, Canada
326 Waterloo Institute of Nanotechnology, University of Waterloo, 200 University Ave, N2L3G1, Waterloo, ON, Canada
327 rdf:type schema:Organization
328 grid-institutes:grid.498726.6 schema:alternateName Mediphage Bioceuticals, Inc., 661 University Avenue, Suite 1300, MaRS Centre, West Tower, M5G0B7, Toronto, ON, Canada
329 schema:name Mediphage Bioceuticals, Inc., 661 University Avenue, Suite 1300, MaRS Centre, West Tower, M5G0B7, Toronto, ON, Canada
330 School of Pharmacy, University of Waterloo, 200 University Ave, N2L3G1, Waterloo, ON, Canada
331 Waterloo Institute of Nanotechnology, University of Waterloo, 200 University Ave, N2L3G1, Waterloo, ON, Canada
332 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...