Testosterone stimulates glucose uptake and GLUT4 translocation through LKB1/AMPK signaling in 3T3-L1 adipocytes View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2015-06-23

AUTHORS

Kazuteru Mitsuhashi, Takafumi Senmaru, Takuya Fukuda, Masahiro Yamazaki, Katsuhiko Shinomiya, Morio Ueno, Shigeru Kinoshita, Jo Kitawaki, Masato Katsuyama, Muneo Tsujikawa, Hiroshi Obayashi, Naoto Nakamura, Michiaki Fukui

ABSTRACT

Decreases in serum testosterone concentrations in aging men are associated with metabolic disorders. Testosterone has been reported to increase GLUT4-dependent glucose uptake in skeletal muscle cells and cardiomyocytes. However, studies on glucose uptake occurring in response to testosterone stimulation in adipocytes are currently not available. This study was designed to determine the effects of testosterone on glucose uptake in adipocytes. Glucose uptake was assessed with 2-[3H] deoxyglucose in 3T3-L1 adipocytes. GLUT4 translocation was evaluated in plasma membrane (PM) sheets and PM fractions by immunofluorescence and immunoblotting, respectively. Activation of GLUT4 translocation-related protein kinases, including Akt, AMPK, LKB1, CaMKI, CaMKII, and Cbl was followed by immunoblotting. Expression levels of androgen receptor (AR) mRNA and AR translocation to the PM were assessed by real-time RT-PCR and immunoblotting, respectively. The results showed that both high-dose (100 nM) testosterone and testosterone-BSA increased glucose uptake and GLUT4 translocation to the PM, independently of the intracellular AR. Testosterone and testosterone-BSA stimulated the phosphorylation of AMPK, LKB1, and CaMKII. The knockdown of LKB1 by siRNA attenuated testosterone- and testosterone-BSA-stimulated AMPK phosphorylation and glucose uptake. These results indicate that high-dose testosterone and testosterone-BSA increase GLUT4-dependent glucose uptake in 3T3-L1 adipocytes by inducing the LKB1/AMPK signaling pathway. More... »

PAGES

174-184

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s12020-015-0666-y

DOI

http://dx.doi.org/10.1007/s12020-015-0666-y

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1044220119

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/26100787


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55 effects of testosterone
56 expression levels
57 fraction
58 glucose uptake
59 high-dose testosterone
60 immunoblotting
61 immunofluorescence
62 kinase
63 knockdown
64 knockdown of LKB1
65 levels
66 mRNA
67 membrane sheets
68 men
69 metabolic disorders
70 muscle cells
71 pathway
72 phosphorylation
73 phosphorylation of AMPK
74 plasma membrane sheets
75 protein kinase
76 real-time RT-PCR
77 receptor mRNA
78 response
79 results
80 serum testosterone concentrations
81 sheets
82 siRNA
83 skeletal muscle cells
84 stimulation
85 study
86 testosterone
87 testosterone concentrations
88 testosterone stimulation
89 testosterone-BSA
90 translocation
91 uptake
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