Human Placental Mesenchymal Stem Cells (pMSCs) Play a Role as Immune Suppressive Cells by Shifting Macrophage Differentiation from Inflammatory M1 ... View Full Text


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Article Info

DATE

2013-06-28

AUTHORS

M. H. Abumaree, M. A. Al Jumah, B. Kalionis, D. Jawdat, A. Al Khaldi, F. M. Abomaray, A. S. Fatani, L. W. Chamley, B. A. Knawy

ABSTRACT

BackgroundMesenchymal stem cells (MSCs) have a therapeutic potential in tissue repair because of capacity for multipotent differentiation and their ability to modulate the immune response. In this study, we examined the ability of human placental MSCs (pMSCs) to modify the differentiation of human monocytes into macrophages and assessed the influence of pMSCs on important macrophage functions.MethodsWe used GM-CSF to stimulate the differentiation of monocytes into the M1 macrophage pathway and then co-cultured these cells with pMSCs in the early stages of macrophage differentiation. We then evaluated the effect on differentiation by microscopic examination and by quantification of molecules important in the differentiation and immune functions of macrophages using flow cytometry and ELISA. The mechanism by which pMSCs could mediate their effects on macrophage differentiation was also studied.ResultsThe co-culture of pMSCs with monocytes stimulated to follow the inflammatory M1 macrophage differentiation pathway resulted in a shift to anti-inflammatory M2-like macrophage differentiation. This transition was characterized by morphological of changes typical of M2 macrophages, and by changes in cell surface marker expression including CD14, CD36, CD163, CD204, CD206, B7-H4 and CD11b, which are distinctive of M2 macrophages. Co-culture with pMSCs reduced the expression of the costimulatory molecules (CD40, CD80 and CD86) and increased the expression of co-inhibitory molecules (CD273, CD274 and B7-H4) as well as the surface expression of major histocompatibility complex (MHC-II) molecules. Furthermore, the secretion of IL-10 was increased while the secretion of IL-1β, IL-12 (p70) and MIP-1α was decreased; a profile typical of M2 macrophages. Finally, pMSCs induced the phagocytic activity and the phagocytosis of apoptotic cells associated with M2- like macrophages; again a profile typical of M2 macrophages. We found that the immunoregulatory effect of pMSCs on macrophage differentiation was mediated by soluble molecules acting partially via glucocorticoid and progesterone receptors.ConclusionsWe have shown that pMSCs can transition macrophages from an inflammatory M1 into an anti-inflammatory M2 phenotype. Our findings suggest a new immunosuppressive property of pMSCs that may be employed in the resolution of inflammation associated with inflammatory diseases and in tissue repair. More... »

PAGES

620-641

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s12015-013-9455-2

    DOI

    http://dx.doi.org/10.1007/s12015-013-9455-2

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1005808579

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/23812784


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    362 King Abdullah International Medical Research Center, King Abdulaziz Medical City, National Guard Health Affairs, P.O. Box 22490, 11426, Mail Code 1515, Riyadh, Saudi Arabia
    363 rdf:type schema:Organization
    364 grid-institutes:grid.416259.d schema:alternateName Department of Perinatal Medicine Pregnancy Research Centre, Royal Women’s Hospital, 3052, Parkville, Victoria, Australia
    365 schema:name Department of Obstetrics and Gynaecology, University of Melbourne, 3052, Parkville, Victoria, Australia
    366 Department of Perinatal Medicine Pregnancy Research Centre, Royal Women’s Hospital, 3052, Parkville, Victoria, Australia
    367 rdf:type schema:Organization
    368 grid-institutes:grid.9654.e schema:alternateName Department of Obstetrics & Gynaecology, Faculty of Medical and Health Sciences, University of Auckland, 11, Private Bag 92019, Grafton, Auckland, New Zealand
    369 schema:name Department of Obstetrics & Gynaecology, Faculty of Medical and Health Sciences, University of Auckland, 11, Private Bag 92019, Grafton, Auckland, New Zealand
    370 rdf:type schema:Organization
     




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