Conformational Changes and Membrane Interaction of the Bacterial Phospholipase, ExoU: Characterization by Site-Directed Spin Labeling View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2019-03

AUTHORS

Jimmy B. Feix, Samantha Kohn, Maxx H. Tessmer, David M. Anderson, Dara W. Frank

ABSTRACT

Numerous pathogenic bacteria produce proteins evolved to facilitate their survival and dissemination by modifying the host environment. These proteins, termed effectors, often play a significant role in determining the virulence of the infection. Consequently, bacterial effectors constitute an important class of targets for the development of novel antibiotics. ExoU is a potent phospholipase effector produced by the opportunistic pathogen Pseudomonas aeruginosa. Previous studies have established that the phospholipase activity of ExoU requires non-covalent interaction with ubiquitin, however the molecular details of the mechanism of activation and the manner in which ExoU associates with a target lipid bilayer are not understood. In this review we describe our recent studies using site-directed spin labeling (SDSL) and EPR spectroscopy to elucidate the conformational changes and membrane interactions that accompany activation of ExoU. We find that ubiquitin binding and membrane interaction act synergistically to produce structural transitions that occur upon ExoU activation, and that the C-terminal four-helix bundle of ExoU functions as a phospholipid-binding domain, facilitating the association of ExoU with the membrane surface. More... »

PAGES

79-87

References to SciGraph publications

  • 2017-12. Structural Dynamics of the MscL C-terminal Domain in SCIENTIFIC REPORTS
  • 2008-02. Membrane recognition by phospholipid-binding domains in NATURE REVIEWS MOLECULAR CELL BIOLOGY
  • 2006-06. DeerAnalysis2006—a comprehensive software package for analyzing pulsed ELDOR data in APPLIED MAGNETIC RESONANCE
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s12013-018-0851-8

    DOI

    http://dx.doi.org/10.1007/s12013-018-0851-8

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1105830693

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/30047043


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biochemistry and Cell Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Bacterial Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Electron Spin Resonance Spectroscopy", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Liposomes", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Phospholipases", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Protein Binding", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Pseudomonas aeruginosa", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Spin Labels", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Ubiquitin", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Medical College of Wisconsin", 
              "id": "https://www.grid.ac/institutes/grid.30760.32", 
              "name": [
                "Department of Biophysics, Medical College of Wisconsin, 53226, Milwaukee, WI, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Feix", 
            "givenName": "Jimmy B.", 
            "id": "sg:person.0740752410.83", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0740752410.83"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Medical College of Wisconsin", 
              "id": "https://www.grid.ac/institutes/grid.30760.32", 
              "name": [
                "Department of Biophysics, Medical College of Wisconsin, 53226, Milwaukee, WI, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kohn", 
            "givenName": "Samantha", 
            "id": "sg:person.013153433240.50", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013153433240.50"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Medical College of Wisconsin", 
              "id": "https://www.grid.ac/institutes/grid.30760.32", 
              "name": [
                "Department of Microbiology and Immunology, Medical College of Wisconsin, 53226, Milwaukee, WI, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Tessmer", 
            "givenName": "Maxx H.", 
            "id": "sg:person.012272174535.91", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012272174535.91"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Vanderbilt University", 
              "id": "https://www.grid.ac/institutes/grid.152326.1", 
              "name": [
                "Department of Pathology, Microbiology and Immunology, Vanderbilt University, 37232, Nashville, TN, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Anderson", 
            "givenName": "David M.", 
            "id": "sg:person.01066467366.05", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01066467366.05"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Medical College of Wisconsin", 
              "id": "https://www.grid.ac/institutes/grid.30760.32", 
              "name": [
                "Department of Microbiology and Immunology, Medical College of Wisconsin, 53226, Milwaukee, WI, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Frank", 
            "givenName": "Dara W.", 
            "id": "sg:person.0707766666.47", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0707766666.47"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1371/journal.ppat.1002637", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001714319"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/jb.00904-09", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001814100"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/emboj/cdg290", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001901828"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1462-5822.2006.00710.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003906293"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1462-5822.2006.00710.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003906293"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrm2328", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004046070", 
              "https://doi.org/10.1038/nrm2328"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1529/biophysj.105.059055", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005656106"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.bbamem.2014.10.004", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009115698"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/bi0497906", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010448175"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/bi0497906", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010448175"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1146/annurev-physchem-032511-143716", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012078928"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.0908700107", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014030170"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1146/annurev.biophys.33.110502.133337", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014972039"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/jb.187.3.1192-1195.2005", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015823811"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1086/421946", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016269125"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1097/00003246-200203000-00005", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017099034"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1097/00003246-200203000-00005", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017099034"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1097/00003246-200203000-00005", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017099034"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.0802515105", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017396256"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1074/jbc.m302472200", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019381074"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pone.0103127", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019536594"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1046/j.1365-2958.1997.4891851.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021117542"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1046/j.1365-2958.1997.4891851.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021117542"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/cr200085w", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021881756"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/cr200085w", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021881756"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0091-679x(07)84020-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023791962"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0006-3495(04)74108-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023811922"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf03166213", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024213766", 
              "https://doi.org/10.1007/bf03166213"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf03166213", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024213766", 
              "https://doi.org/10.1007/bf03166213"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.91.5.1667", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025157938"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pone.0049388", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026493189"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.sbi.2006.08.008", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028734523"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/bs.mie.2015.08.006", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030852989"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.str.2011.10.009", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032516954"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/iai.00414-13", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033427843"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.bpj.2011.01.056", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035835117"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.sbi.2013.06.008", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036434195"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/anie.201609617", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038547398"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/bi9826310", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039348471"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/bi9826310", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039348471"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1086/320737", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040016466"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1365-2958.2011.07904.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040135366"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1529/biophysj.105.059063", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040630041"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/iai.73.1.573-582.2005", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040687747"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/iai.74.5.2552-2561.2006", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042010779"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1462-5822.2011.01718.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042970779"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.284.5418.1322", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043411147"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1146/annurev-micro-092412-155725", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043527004"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1529/biophysj.107.104034", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043625074"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/bs.mie.2015.07.026", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045342956"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0006-3495(89)82765-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046471298"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1006/jmre.1999.1944", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049796044"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.jmr.2010.08.002", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050522308"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/iai.00223-10", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050804303"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.bpj.2009.12.4193", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051079727"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0003-2697(82)90612-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1052142275"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1097/ccm.0b013e318298a86f", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053243792"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1097/ccm.0b013e318298a86f", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053243792"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/bi002645h", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055167658"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/bi002645h", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055167658"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/bi0160821", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055195133"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/bi0160821", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055195133"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/bi0268145", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055196386"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/bi0268145", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055196386"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/bi400834a", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055206097"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/bi800711t", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055208627"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/bi800711t", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055208627"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/bi971232m", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055214179"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/bi971232m", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055214179"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1074/jbc.m116.760074", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1058215728"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1101373", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062450893"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.3389/fcimb.2017.00039", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1083824090"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.1620405114", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1084510629"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/s41598-017-17396-w", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1093156882", 
              "https://doi.org/10.1038/s41598-017-17396-w"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.1716861115", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1100155635"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.bpj.2017.11.3759", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1100858321"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2019-03", 
        "datePublishedReg": "2019-03-01", 
        "description": "Numerous pathogenic bacteria produce proteins evolved to facilitate their survival and dissemination by modifying the host environment. These proteins, termed effectors, often play a significant role in determining the virulence of the infection. Consequently, bacterial effectors constitute an important class of targets for the development of novel antibiotics. ExoU is a potent phospholipase effector produced by the opportunistic pathogen Pseudomonas aeruginosa. Previous studies have established that the phospholipase activity of ExoU requires non-covalent interaction with ubiquitin, however the molecular details of the mechanism of activation and the manner in which ExoU associates with a target lipid bilayer are not understood. In this review we describe our recent studies using site-directed spin labeling (SDSL) and EPR spectroscopy to elucidate the conformational changes and membrane interactions that accompany activation of ExoU. We find that ubiquitin binding and membrane interaction act synergistically to produce structural transitions that occur upon ExoU activation, and that the C-terminal four-helix bundle of ExoU functions as a phospholipid-binding domain, facilitating the association of ExoU with the membrane surface.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1007/s12013-018-0851-8", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.4103617", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.2674355", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.2671470", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.2459868", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1090910", 
            "issn": [
              "1085-9195", 
              "1559-0283"
            ], 
            "name": "Cell Biochemistry and Biophysics", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "77"
          }
        ], 
        "name": "Conformational Changes and Membrane Interaction of the Bacterial Phospholipase, ExoU: Characterization by Site-Directed Spin Labeling", 
        "pagination": "79-87", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "9ee11f4ba76f419f6d746b9eff0ff99674f5dab1792442b74cc1a6ce68ab38e5"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "30047043"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "9701934"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/s12013-018-0851-8"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1105830693"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/s12013-018-0851-8", 
          "https://app.dimensions.ai/details/publication/pub.1105830693"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-11T14:17", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000372_0000000372/records_117091_00000003.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://link.springer.com/10.1007%2Fs12013-018-0851-8"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s12013-018-0851-8'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s12013-018-0851-8'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s12013-018-0851-8'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s12013-018-0851-8'


     

    This table displays all metadata directly associated to this object as RDF triples.

    330 TRIPLES      21 PREDICATES      99 URIs      29 LITERALS      17 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/s12013-018-0851-8 schema:about N239e2cfc63ea43e69db57258de790476
    2 N29de46bfb01a4a959594f3366ef350e6
    3 N4c4d8e88921d4ec8a6529df451c64497
    4 N7002ee8adccd4975b0e1cdcb1b821e9d
    5 N8e2187465a2845e5ae5d9888d775c76e
    6 N9f4fc055131f4d9a8a2aacb5c9167d11
    7 Nc9ecd36d4ae04675b31ae74f83c90760
    8 Ndd34d5f600d94d6b8107ea559058f6f9
    9 anzsrc-for:06
    10 anzsrc-for:0601
    11 schema:author N3157c2daf48e42a982d4740004b62d35
    12 schema:citation sg:pub.10.1007/bf03166213
    13 sg:pub.10.1038/nrm2328
    14 sg:pub.10.1038/s41598-017-17396-w
    15 https://doi.org/10.1002/anie.201609617
    16 https://doi.org/10.1006/jmre.1999.1944
    17 https://doi.org/10.1016/0003-2697(82)90612-1
    18 https://doi.org/10.1016/bs.mie.2015.07.026
    19 https://doi.org/10.1016/bs.mie.2015.08.006
    20 https://doi.org/10.1016/j.bbamem.2014.10.004
    21 https://doi.org/10.1016/j.bpj.2009.12.4193
    22 https://doi.org/10.1016/j.bpj.2011.01.056
    23 https://doi.org/10.1016/j.bpj.2017.11.3759
    24 https://doi.org/10.1016/j.jmr.2010.08.002
    25 https://doi.org/10.1016/j.sbi.2006.08.008
    26 https://doi.org/10.1016/j.sbi.2013.06.008
    27 https://doi.org/10.1016/j.str.2011.10.009
    28 https://doi.org/10.1016/s0006-3495(04)74108-9
    29 https://doi.org/10.1016/s0006-3495(89)82765-1
    30 https://doi.org/10.1016/s0091-679x(07)84020-9
    31 https://doi.org/10.1021/bi002645h
    32 https://doi.org/10.1021/bi0160821
    33 https://doi.org/10.1021/bi0268145
    34 https://doi.org/10.1021/bi0497906
    35 https://doi.org/10.1021/bi400834a
    36 https://doi.org/10.1021/bi800711t
    37 https://doi.org/10.1021/bi971232m
    38 https://doi.org/10.1021/bi9826310
    39 https://doi.org/10.1021/cr200085w
    40 https://doi.org/10.1046/j.1365-2958.1997.4891851.x
    41 https://doi.org/10.1073/pnas.0802515105
    42 https://doi.org/10.1073/pnas.0908700107
    43 https://doi.org/10.1073/pnas.1620405114
    44 https://doi.org/10.1073/pnas.1716861115
    45 https://doi.org/10.1073/pnas.91.5.1667
    46 https://doi.org/10.1074/jbc.m116.760074
    47 https://doi.org/10.1074/jbc.m302472200
    48 https://doi.org/10.1086/320737
    49 https://doi.org/10.1086/421946
    50 https://doi.org/10.1093/emboj/cdg290
    51 https://doi.org/10.1097/00003246-200203000-00005
    52 https://doi.org/10.1097/ccm.0b013e318298a86f
    53 https://doi.org/10.1111/j.1365-2958.2011.07904.x
    54 https://doi.org/10.1111/j.1462-5822.2006.00710.x
    55 https://doi.org/10.1111/j.1462-5822.2011.01718.x
    56 https://doi.org/10.1126/science.1101373
    57 https://doi.org/10.1126/science.284.5418.1322
    58 https://doi.org/10.1128/iai.00223-10
    59 https://doi.org/10.1128/iai.00414-13
    60 https://doi.org/10.1128/iai.73.1.573-582.2005
    61 https://doi.org/10.1128/iai.74.5.2552-2561.2006
    62 https://doi.org/10.1128/jb.00904-09
    63 https://doi.org/10.1128/jb.187.3.1192-1195.2005
    64 https://doi.org/10.1146/annurev-micro-092412-155725
    65 https://doi.org/10.1146/annurev-physchem-032511-143716
    66 https://doi.org/10.1146/annurev.biophys.33.110502.133337
    67 https://doi.org/10.1371/journal.pone.0049388
    68 https://doi.org/10.1371/journal.pone.0103127
    69 https://doi.org/10.1371/journal.ppat.1002637
    70 https://doi.org/10.1529/biophysj.105.059055
    71 https://doi.org/10.1529/biophysj.105.059063
    72 https://doi.org/10.1529/biophysj.107.104034
    73 https://doi.org/10.3389/fcimb.2017.00039
    74 schema:datePublished 2019-03
    75 schema:datePublishedReg 2019-03-01
    76 schema:description Numerous pathogenic bacteria produce proteins evolved to facilitate their survival and dissemination by modifying the host environment. These proteins, termed effectors, often play a significant role in determining the virulence of the infection. Consequently, bacterial effectors constitute an important class of targets for the development of novel antibiotics. ExoU is a potent phospholipase effector produced by the opportunistic pathogen Pseudomonas aeruginosa. Previous studies have established that the phospholipase activity of ExoU requires non-covalent interaction with ubiquitin, however the molecular details of the mechanism of activation and the manner in which ExoU associates with a target lipid bilayer are not understood. In this review we describe our recent studies using site-directed spin labeling (SDSL) and EPR spectroscopy to elucidate the conformational changes and membrane interactions that accompany activation of ExoU. We find that ubiquitin binding and membrane interaction act synergistically to produce structural transitions that occur upon ExoU activation, and that the C-terminal four-helix bundle of ExoU functions as a phospholipid-binding domain, facilitating the association of ExoU with the membrane surface.
    77 schema:genre research_article
    78 schema:inLanguage en
    79 schema:isAccessibleForFree false
    80 schema:isPartOf N3edf5f50d7ad459f9e0ece1e0275cf50
    81 Nc4f08d6ebc9b464fb24e906fdbf997ce
    82 sg:journal.1090910
    83 schema:name Conformational Changes and Membrane Interaction of the Bacterial Phospholipase, ExoU: Characterization by Site-Directed Spin Labeling
    84 schema:pagination 79-87
    85 schema:productId N6a6cd5f90ebd4619a5778c27998ec89a
    86 N78deb8eef74f43a1ada7209a21ff5cd7
    87 N928c5f24e6f7436f866727427bc675c6
    88 Nc8faed2218f84e2d99d5240a8778005d
    89 Nd191c1716a664890831f560931243fd3
    90 schema:sameAs https://app.dimensions.ai/details/publication/pub.1105830693
    91 https://doi.org/10.1007/s12013-018-0851-8
    92 schema:sdDatePublished 2019-04-11T14:17
    93 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    94 schema:sdPublisher Na78237cc7d5c453cb8b07b3fed2ac8b7
    95 schema:url https://link.springer.com/10.1007%2Fs12013-018-0851-8
    96 sgo:license sg:explorer/license/
    97 sgo:sdDataset articles
    98 rdf:type schema:ScholarlyArticle
    99 N239e2cfc63ea43e69db57258de790476 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    100 schema:name Ubiquitin
    101 rdf:type schema:DefinedTerm
    102 N29de46bfb01a4a959594f3366ef350e6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    103 schema:name Pseudomonas aeruginosa
    104 rdf:type schema:DefinedTerm
    105 N3157c2daf48e42a982d4740004b62d35 rdf:first sg:person.0740752410.83
    106 rdf:rest Ne771690a89ef40ae95c8d0b396c8c7f8
    107 N3edf5f50d7ad459f9e0ece1e0275cf50 schema:volumeNumber 77
    108 rdf:type schema:PublicationVolume
    109 N4136e35b06294042af82c0b37503ae62 rdf:first sg:person.01066467366.05
    110 rdf:rest N6f1768baba0b4e77aee07601e1bb3dfd
    111 N4c4d8e88921d4ec8a6529df451c64497 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    112 schema:name Bacterial Proteins
    113 rdf:type schema:DefinedTerm
    114 N6a6cd5f90ebd4619a5778c27998ec89a schema:name pubmed_id
    115 schema:value 30047043
    116 rdf:type schema:PropertyValue
    117 N6d27f9bde8794de88d96a1bbae158ab3 rdf:first sg:person.012272174535.91
    118 rdf:rest N4136e35b06294042af82c0b37503ae62
    119 N6f1768baba0b4e77aee07601e1bb3dfd rdf:first sg:person.0707766666.47
    120 rdf:rest rdf:nil
    121 N7002ee8adccd4975b0e1cdcb1b821e9d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    122 schema:name Liposomes
    123 rdf:type schema:DefinedTerm
    124 N78deb8eef74f43a1ada7209a21ff5cd7 schema:name doi
    125 schema:value 10.1007/s12013-018-0851-8
    126 rdf:type schema:PropertyValue
    127 N8e2187465a2845e5ae5d9888d775c76e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    128 schema:name Electron Spin Resonance Spectroscopy
    129 rdf:type schema:DefinedTerm
    130 N928c5f24e6f7436f866727427bc675c6 schema:name readcube_id
    131 schema:value 9ee11f4ba76f419f6d746b9eff0ff99674f5dab1792442b74cc1a6ce68ab38e5
    132 rdf:type schema:PropertyValue
    133 N9f4fc055131f4d9a8a2aacb5c9167d11 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    134 schema:name Spin Labels
    135 rdf:type schema:DefinedTerm
    136 Na78237cc7d5c453cb8b07b3fed2ac8b7 schema:name Springer Nature - SN SciGraph project
    137 rdf:type schema:Organization
    138 Nc4f08d6ebc9b464fb24e906fdbf997ce schema:issueNumber 1
    139 rdf:type schema:PublicationIssue
    140 Nc8faed2218f84e2d99d5240a8778005d schema:name dimensions_id
    141 schema:value pub.1105830693
    142 rdf:type schema:PropertyValue
    143 Nc9ecd36d4ae04675b31ae74f83c90760 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    144 schema:name Protein Binding
    145 rdf:type schema:DefinedTerm
    146 Nd191c1716a664890831f560931243fd3 schema:name nlm_unique_id
    147 schema:value 9701934
    148 rdf:type schema:PropertyValue
    149 Ndd34d5f600d94d6b8107ea559058f6f9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    150 schema:name Phospholipases
    151 rdf:type schema:DefinedTerm
    152 Ne771690a89ef40ae95c8d0b396c8c7f8 rdf:first sg:person.013153433240.50
    153 rdf:rest N6d27f9bde8794de88d96a1bbae158ab3
    154 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    155 schema:name Biological Sciences
    156 rdf:type schema:DefinedTerm
    157 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
    158 schema:name Biochemistry and Cell Biology
    159 rdf:type schema:DefinedTerm
    160 sg:grant.2459868 http://pending.schema.org/fundedItem sg:pub.10.1007/s12013-018-0851-8
    161 rdf:type schema:MonetaryGrant
    162 sg:grant.2671470 http://pending.schema.org/fundedItem sg:pub.10.1007/s12013-018-0851-8
    163 rdf:type schema:MonetaryGrant
    164 sg:grant.2674355 http://pending.schema.org/fundedItem sg:pub.10.1007/s12013-018-0851-8
    165 rdf:type schema:MonetaryGrant
    166 sg:grant.4103617 http://pending.schema.org/fundedItem sg:pub.10.1007/s12013-018-0851-8
    167 rdf:type schema:MonetaryGrant
    168 sg:journal.1090910 schema:issn 1085-9195
    169 1559-0283
    170 schema:name Cell Biochemistry and Biophysics
    171 rdf:type schema:Periodical
    172 sg:person.01066467366.05 schema:affiliation https://www.grid.ac/institutes/grid.152326.1
    173 schema:familyName Anderson
    174 schema:givenName David M.
    175 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01066467366.05
    176 rdf:type schema:Person
    177 sg:person.012272174535.91 schema:affiliation https://www.grid.ac/institutes/grid.30760.32
    178 schema:familyName Tessmer
    179 schema:givenName Maxx H.
    180 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012272174535.91
    181 rdf:type schema:Person
    182 sg:person.013153433240.50 schema:affiliation https://www.grid.ac/institutes/grid.30760.32
    183 schema:familyName Kohn
    184 schema:givenName Samantha
    185 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013153433240.50
    186 rdf:type schema:Person
    187 sg:person.0707766666.47 schema:affiliation https://www.grid.ac/institutes/grid.30760.32
    188 schema:familyName Frank
    189 schema:givenName Dara W.
    190 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0707766666.47
    191 rdf:type schema:Person
    192 sg:person.0740752410.83 schema:affiliation https://www.grid.ac/institutes/grid.30760.32
    193 schema:familyName Feix
    194 schema:givenName Jimmy B.
    195 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0740752410.83
    196 rdf:type schema:Person
    197 sg:pub.10.1007/bf03166213 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024213766
    198 https://doi.org/10.1007/bf03166213
    199 rdf:type schema:CreativeWork
    200 sg:pub.10.1038/nrm2328 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004046070
    201 https://doi.org/10.1038/nrm2328
    202 rdf:type schema:CreativeWork
    203 sg:pub.10.1038/s41598-017-17396-w schema:sameAs https://app.dimensions.ai/details/publication/pub.1093156882
    204 https://doi.org/10.1038/s41598-017-17396-w
    205 rdf:type schema:CreativeWork
    206 https://doi.org/10.1002/anie.201609617 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038547398
    207 rdf:type schema:CreativeWork
    208 https://doi.org/10.1006/jmre.1999.1944 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049796044
    209 rdf:type schema:CreativeWork
    210 https://doi.org/10.1016/0003-2697(82)90612-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052142275
    211 rdf:type schema:CreativeWork
    212 https://doi.org/10.1016/bs.mie.2015.07.026 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045342956
    213 rdf:type schema:CreativeWork
    214 https://doi.org/10.1016/bs.mie.2015.08.006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030852989
    215 rdf:type schema:CreativeWork
    216 https://doi.org/10.1016/j.bbamem.2014.10.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009115698
    217 rdf:type schema:CreativeWork
    218 https://doi.org/10.1016/j.bpj.2009.12.4193 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051079727
    219 rdf:type schema:CreativeWork
    220 https://doi.org/10.1016/j.bpj.2011.01.056 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035835117
    221 rdf:type schema:CreativeWork
    222 https://doi.org/10.1016/j.bpj.2017.11.3759 schema:sameAs https://app.dimensions.ai/details/publication/pub.1100858321
    223 rdf:type schema:CreativeWork
    224 https://doi.org/10.1016/j.jmr.2010.08.002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050522308
    225 rdf:type schema:CreativeWork
    226 https://doi.org/10.1016/j.sbi.2006.08.008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028734523
    227 rdf:type schema:CreativeWork
    228 https://doi.org/10.1016/j.sbi.2013.06.008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036434195
    229 rdf:type schema:CreativeWork
    230 https://doi.org/10.1016/j.str.2011.10.009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032516954
    231 rdf:type schema:CreativeWork
    232 https://doi.org/10.1016/s0006-3495(04)74108-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023811922
    233 rdf:type schema:CreativeWork
    234 https://doi.org/10.1016/s0006-3495(89)82765-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046471298
    235 rdf:type schema:CreativeWork
    236 https://doi.org/10.1016/s0091-679x(07)84020-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023791962
    237 rdf:type schema:CreativeWork
    238 https://doi.org/10.1021/bi002645h schema:sameAs https://app.dimensions.ai/details/publication/pub.1055167658
    239 rdf:type schema:CreativeWork
    240 https://doi.org/10.1021/bi0160821 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055195133
    241 rdf:type schema:CreativeWork
    242 https://doi.org/10.1021/bi0268145 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055196386
    243 rdf:type schema:CreativeWork
    244 https://doi.org/10.1021/bi0497906 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010448175
    245 rdf:type schema:CreativeWork
    246 https://doi.org/10.1021/bi400834a schema:sameAs https://app.dimensions.ai/details/publication/pub.1055206097
    247 rdf:type schema:CreativeWork
    248 https://doi.org/10.1021/bi800711t schema:sameAs https://app.dimensions.ai/details/publication/pub.1055208627
    249 rdf:type schema:CreativeWork
    250 https://doi.org/10.1021/bi971232m schema:sameAs https://app.dimensions.ai/details/publication/pub.1055214179
    251 rdf:type schema:CreativeWork
    252 https://doi.org/10.1021/bi9826310 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039348471
    253 rdf:type schema:CreativeWork
    254 https://doi.org/10.1021/cr200085w schema:sameAs https://app.dimensions.ai/details/publication/pub.1021881756
    255 rdf:type schema:CreativeWork
    256 https://doi.org/10.1046/j.1365-2958.1997.4891851.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1021117542
    257 rdf:type schema:CreativeWork
    258 https://doi.org/10.1073/pnas.0802515105 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017396256
    259 rdf:type schema:CreativeWork
    260 https://doi.org/10.1073/pnas.0908700107 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014030170
    261 rdf:type schema:CreativeWork
    262 https://doi.org/10.1073/pnas.1620405114 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084510629
    263 rdf:type schema:CreativeWork
    264 https://doi.org/10.1073/pnas.1716861115 schema:sameAs https://app.dimensions.ai/details/publication/pub.1100155635
    265 rdf:type schema:CreativeWork
    266 https://doi.org/10.1073/pnas.91.5.1667 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025157938
    267 rdf:type schema:CreativeWork
    268 https://doi.org/10.1074/jbc.m116.760074 schema:sameAs https://app.dimensions.ai/details/publication/pub.1058215728
    269 rdf:type schema:CreativeWork
    270 https://doi.org/10.1074/jbc.m302472200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019381074
    271 rdf:type schema:CreativeWork
    272 https://doi.org/10.1086/320737 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040016466
    273 rdf:type schema:CreativeWork
    274 https://doi.org/10.1086/421946 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016269125
    275 rdf:type schema:CreativeWork
    276 https://doi.org/10.1093/emboj/cdg290 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001901828
    277 rdf:type schema:CreativeWork
    278 https://doi.org/10.1097/00003246-200203000-00005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017099034
    279 rdf:type schema:CreativeWork
    280 https://doi.org/10.1097/ccm.0b013e318298a86f schema:sameAs https://app.dimensions.ai/details/publication/pub.1053243792
    281 rdf:type schema:CreativeWork
    282 https://doi.org/10.1111/j.1365-2958.2011.07904.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1040135366
    283 rdf:type schema:CreativeWork
    284 https://doi.org/10.1111/j.1462-5822.2006.00710.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1003906293
    285 rdf:type schema:CreativeWork
    286 https://doi.org/10.1111/j.1462-5822.2011.01718.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1042970779
    287 rdf:type schema:CreativeWork
    288 https://doi.org/10.1126/science.1101373 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062450893
    289 rdf:type schema:CreativeWork
    290 https://doi.org/10.1126/science.284.5418.1322 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043411147
    291 rdf:type schema:CreativeWork
    292 https://doi.org/10.1128/iai.00223-10 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050804303
    293 rdf:type schema:CreativeWork
    294 https://doi.org/10.1128/iai.00414-13 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033427843
    295 rdf:type schema:CreativeWork
    296 https://doi.org/10.1128/iai.73.1.573-582.2005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040687747
    297 rdf:type schema:CreativeWork
    298 https://doi.org/10.1128/iai.74.5.2552-2561.2006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042010779
    299 rdf:type schema:CreativeWork
    300 https://doi.org/10.1128/jb.00904-09 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001814100
    301 rdf:type schema:CreativeWork
    302 https://doi.org/10.1128/jb.187.3.1192-1195.2005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015823811
    303 rdf:type schema:CreativeWork
    304 https://doi.org/10.1146/annurev-micro-092412-155725 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043527004
    305 rdf:type schema:CreativeWork
    306 https://doi.org/10.1146/annurev-physchem-032511-143716 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012078928
    307 rdf:type schema:CreativeWork
    308 https://doi.org/10.1146/annurev.biophys.33.110502.133337 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014972039
    309 rdf:type schema:CreativeWork
    310 https://doi.org/10.1371/journal.pone.0049388 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026493189
    311 rdf:type schema:CreativeWork
    312 https://doi.org/10.1371/journal.pone.0103127 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019536594
    313 rdf:type schema:CreativeWork
    314 https://doi.org/10.1371/journal.ppat.1002637 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001714319
    315 rdf:type schema:CreativeWork
    316 https://doi.org/10.1529/biophysj.105.059055 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005656106
    317 rdf:type schema:CreativeWork
    318 https://doi.org/10.1529/biophysj.105.059063 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040630041
    319 rdf:type schema:CreativeWork
    320 https://doi.org/10.1529/biophysj.107.104034 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043625074
    321 rdf:type schema:CreativeWork
    322 https://doi.org/10.3389/fcimb.2017.00039 schema:sameAs https://app.dimensions.ai/details/publication/pub.1083824090
    323 rdf:type schema:CreativeWork
    324 https://www.grid.ac/institutes/grid.152326.1 schema:alternateName Vanderbilt University
    325 schema:name Department of Pathology, Microbiology and Immunology, Vanderbilt University, 37232, Nashville, TN, USA
    326 rdf:type schema:Organization
    327 https://www.grid.ac/institutes/grid.30760.32 schema:alternateName Medical College of Wisconsin
    328 schema:name Department of Biophysics, Medical College of Wisconsin, 53226, Milwaukee, WI, USA
    329 Department of Microbiology and Immunology, Medical College of Wisconsin, 53226, Milwaukee, WI, USA
    330 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...