Study on the Diversity of Bacteroides and Clostridium in Patients with Primary Gout View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2014-10-26

AUTHORS

Shi-Chao Xing, Dong-Mei Meng, Ying Chen, Gang Jiang, Xi-Shuang Liu, Na Li, Yao-Yao Yan, Chang-Gui Li

ABSTRACT

To analyze the diversity of both Bacteroides and Clostridium in patients with primary gout and the difference from that of normal individuals. And to investigate the relationship between the primary gout and the intestinal flora. Fecal samples of 90 cases with the primary gout and 94 cases normal comparison group were selected, together with the cases that match the filter criteria. The DNA is extracted from the feces. 16S rRNA specific primers of both Bacteroides and Clostridium were adopted for the PCR amplification. The molecular fingerprints of Bacteroides and Clostridium in both the primary gout group and the normal control group were obtained through DGGE and subjected for further analysis on both the diversity and the similarity. Compared with normal individuals, the number of bands and Shannon–Weaver (H′) of Bacteroides in patients with primary gout was not reduced, but significantly decreased in Clostridium. Furthermore, the intra-group and inter-group similarity of both Bacteroides and Clostridium were lower. The primary gout has caused the structural change of both Bacteroides and Clostridium, inducing the low similarity, especially for Clostridium. It has statistic significance. The gut predominant flora may play an important role in the development of primary gout. More... »

PAGES

707-715

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s12013-014-0253-5

DOI

http://dx.doi.org/10.1007/s12013-014-0253-5

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1035200000

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/25344643


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