Screening of Fungi Isolates for C-4 Hydroxylation of R-2-Phenoxypropionic Acid Based on a Novel 96-Well Microplate Assay Method View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2020-03-25

AUTHORS

Hai-Yan Zhou, Rui Jiang, Yi-Zuo Li, Wei Xu, Yuan-Shan Wang, Ya-Ping Xue, Yu-Guo Zheng

ABSTRACT

R-2-(4-Hydroxyphenoxy)propionic acid (R-HPPA) is a pivotal intermediate for the synthesis of aryloxyphenoxypropionate (APP) herbicide. To rapidly screen microbial isolates with the capacity of hydroxylating R-2-phenoxypropionic acid to R-HPPA from various environmental samples, a convenient and safe 96-well microplate assay method with sodium nitrite (NaNO2) as chromogenic reagent was proposed and optimized. The optimum assay conditions were as follows: the detection wavelength was 420 nm, the concentration of NaNO2 solution was 6.0 g/L, color reaction temperature was 60 °C, the pH of the NaNO2 solution was 2.4, and the reaction time was 40 min. With the aid of this method, screening for microorganisms with C-4-specific hydroxylation activity of R-PPA was conducted. As a result, 23 strains among 3744 single colonies isolated from various samples exhibited the hydroxylation activity. Among these strains, the highest bioconversion rate was achieved by Penicillium oxalicum A5 and Aspergillus versicolor A12, respectively. After 72-h cultivation in shake flask, their conversion rates of R-HPPA from 10 g/L R-PPA reached 21.18% and 40.24%, respectively. The established method was effective in rapid screening of microbes capable of biosynthesizing R-HPPA through hydroxylation of R-PPA, and the obtained two fungi species could be potentially used for R-HPPA production. More... »

PAGES

42-56

References to SciGraph publications

  • 2019-06-12. A high-throughput screening method for improved R-2-(4-hydroxyphenoxy)propionic acid biosynthesis in BIOPROCESS AND BIOSYSTEMS ENGINEERING
  • 2015-04-14. Efficient production and evaluation of lignocellulolytic enzymes using a constitutive protein expression system in Penicillium oxalicum in JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
  • 2009-11-26. Microbial conversion of 5-sulfoisophthalic acid into 5-hydroxyisophthalic acid by Ochrobactrum anthropi S9 in BIOTECHNOLOGY LETTERS
  • 2018-03-14. High-throughput screening of Mucoromycota fungi for production of low- and high-value lipids in BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS
  • 2014-11-18. Insights into high-efficiency lignocellulolytic enzyme production by Penicillium oxalicum GZ-2 induced by a complex substrate in BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS
  • 2017-12-09. Evaluation of Industrial Biocides on a Novel Aspergillus versicolor TANCK-1 and Elucidation of Their Probable Biocidal Mechanism in INDIAN JOURNAL OF MICROBIOLOGY
  • 2017-01-30. Development of a method for efficient cost-effective screening of Aspergillus niger mutants having increased production of glucoamylase in BIOTECHNOLOGY LETTERS
  • 2013-10-10. An Evaluation of the Proteolytic and Lipolytic Potential of Penicillium spp. Isolated from Traditional Greek Sausages in Submerged Fermentation in APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
  • 2015-07-30. Functional diversity and properties of multiple xylanases from Penicillium oxalicum GZ-2 in SCIENTIFIC REPORTS
  • 2015-08-16. Carbazole hydroxylation by the filamentous fungi of the Cunninghamella species in ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
  • 2014-10-01. Differential expression of peroxidase and ABC transporter as the key regulatory components for degradation of azo dyes by Penicillium oxalicum SAR-3 in FUNCTIONAL & INTEGRATIVE GENOMICS
  • 2015-09-14. Evaluation of the efficacy of a fungal consortium for degradation of azo dyes and simulated textile dye effluents in SUSTAINABLE WATER RESOURCES MANAGEMENT
  • 1996-11. A para-site-specific hydroxylation of various aromatic compounds by Mycobacterium sp. strain 12523 in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2008-09-25. Hydroxylation of naphthalene by aromatic peroxygenase from Agrocybe aegerita proceeds via oxygen transfer from H2O2 and intermediary epoxidation in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s12010-020-03303-z

    DOI

    http://dx.doi.org/10.1007/s12010-020-03303-z

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1125905011

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/32212108


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0605", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Microbiology", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Agar", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Animals", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Aspergillus", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Biomass", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Bombyx", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Catalysis", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "China", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "DNA, Ribosomal", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Fermentation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Herbicides", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Hydrogen-Ion Concentration", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Hydroxylation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Penicillium", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Phenyl Ethers", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Phylogeny", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Propionates", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Sewage", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Sodium Nitrite", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Soil Microbiology", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Temperature", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, 18 Chaowang Road, 310014, Hangzhou, People\u2019s Republic of China", 
              "id": "http://www.grid.ac/institutes/grid.469325.f", 
              "name": [
                "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China", 
                "Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China", 
                "The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, 18 Chaowang Road, 310014, Hangzhou, People\u2019s Republic of China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Zhou", 
            "givenName": "Hai-Yan", 
            "id": "sg:person.01274560471.40", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01274560471.40"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, 18 Chaowang Road, 310014, Hangzhou, People\u2019s Republic of China", 
              "id": "http://www.grid.ac/institutes/grid.469325.f", 
              "name": [
                "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China", 
                "Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China", 
                "The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, 18 Chaowang Road, 310014, Hangzhou, People\u2019s Republic of China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Jiang", 
            "givenName": "Rui", 
            "id": "sg:person.016322413273.78", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016322413273.78"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, 18 Chaowang Road, 310014, Hangzhou, People\u2019s Republic of China", 
              "id": "http://www.grid.ac/institutes/grid.469325.f", 
              "name": [
                "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China", 
                "Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China", 
                "The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, 18 Chaowang Road, 310014, Hangzhou, People\u2019s Republic of China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Li", 
            "givenName": "Yi-Zuo", 
            "id": "sg:person.014132071673.75", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014132071673.75"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, 18 Chaowang Road, 310014, Hangzhou, People\u2019s Republic of China", 
              "id": "http://www.grid.ac/institutes/grid.469325.f", 
              "name": [
                "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China", 
                "Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China", 
                "The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, 18 Chaowang Road, 310014, Hangzhou, People\u2019s Republic of China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Xu", 
            "givenName": "Wei", 
            "id": "sg:person.016450272674.19", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016450272674.19"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, 18 Chaowang Road, 310014, Hangzhou, People\u2019s Republic of China", 
              "id": "http://www.grid.ac/institutes/grid.469325.f", 
              "name": [
                "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China", 
                "Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China", 
                "The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, 18 Chaowang Road, 310014, Hangzhou, People\u2019s Republic of China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Wang", 
            "givenName": "Yuan-Shan", 
            "id": "sg:person.0613144457.79", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0613144457.79"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, 18 Chaowang Road, 310014, Hangzhou, People\u2019s Republic of China", 
              "id": "http://www.grid.ac/institutes/grid.469325.f", 
              "name": [
                "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China", 
                "Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China", 
                "The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, 18 Chaowang Road, 310014, Hangzhou, People\u2019s Republic of China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Xue", 
            "givenName": "Ya-Ping", 
            "id": "sg:person.0744724672.04", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0744724672.04"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, 18 Chaowang Road, 310014, Hangzhou, People\u2019s Republic of China", 
              "id": "http://www.grid.ac/institutes/grid.469325.f", 
              "name": [
                "Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China", 
                "Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, 310014, Hangzhou, People\u2019s Republic of China", 
                "The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, 18 Chaowang Road, 310014, Hangzhou, People\u2019s Republic of China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Zheng", 
            "givenName": "Yu-Guo", 
            "id": "sg:person.01144225405.06", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01144225405.06"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1007/s00449-019-02154-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1117065422", 
              "https://doi.org/10.1007/s00449-019-02154-1"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11356-015-5146-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012370203", 
              "https://doi.org/10.1007/s11356-015-5146-7"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s12088-017-0696-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1099701727", 
              "https://doi.org/10.1007/s12088-017-0696-7"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s13068-018-1070-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1101533980", 
              "https://doi.org/10.1186/s13068-018-1070-7"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s12010-013-0570-y", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049527472", 
              "https://doi.org/10.1007/s12010-013-0570-y"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s10529-017-2291-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1083395807", 
              "https://doi.org/10.1007/s10529-017-2291-8"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s13068-014-0162-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021488829", 
              "https://doi.org/10.1186/s13068-014-0162-2"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s10295-015-1607-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040653188", 
              "https://doi.org/10.1007/s10295-015-1607-8"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/srep12631", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038316224", 
              "https://doi.org/10.1038/srep12631"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s10142-014-0405-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010985237", 
              "https://doi.org/10.1007/s10142-014-0405-0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00166241", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053066204", 
              "https://doi.org/10.1007/bf00166241"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s10529-009-0171-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026074431", 
              "https://doi.org/10.1007/s10529-009-0171-6"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00253-008-1704-y", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1027591760", 
              "https://doi.org/10.1007/s00253-008-1704-y"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s40899-015-0027-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022121279", 
              "https://doi.org/10.1007/s40899-015-0027-2"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2020-03-25", 
        "datePublishedReg": "2020-03-25", 
        "description": "R-2-(4-Hydroxyphenoxy)propionic acid (R-HPPA) is a pivotal intermediate for the synthesis of aryloxyphenoxypropionate (APP) herbicide. To rapidly screen microbial isolates with the capacity of hydroxylating R-2-phenoxypropionic acid to R-HPPA from various environmental samples, a convenient and safe 96-well microplate assay method with sodium nitrite (NaNO2) as chromogenic reagent was proposed and optimized. The optimum assay conditions were as follows: the detection wavelength was 420\u00a0nm, the concentration of NaNO2 solution was 6.0\u00a0g/L, color reaction temperature was 60\u00a0\u00b0C, the pH of the NaNO2 solution was 2.4, and the reaction time was 40\u00a0min. With the aid of this method, screening for microorganisms with C-4-specific hydroxylation activity of R-PPA was conducted. As a result, 23 strains among 3744 single colonies isolated from various samples exhibited the hydroxylation activity. Among these strains, the highest bioconversion rate was achieved by Penicillium oxalicum A5 and Aspergillus versicolor A12, respectively. After 72-h cultivation in shake flask, their conversion rates of R-HPPA from 10\u00a0g/L R-PPA reached 21.18% and 40.24%, respectively. The established method was effective in rapid screening of microbes capable of biosynthesizing R-HPPA through hydroxylation of R-PPA, and the obtained two fungi species could be potentially used for R-HPPA production.", 
        "genre": "article", 
        "id": "sg:pub.10.1007/s12010-020-03303-z", 
        "inLanguage": "en", 
        "isAccessibleForFree": false, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.8186142", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1086169", 
            "issn": [
              "0273-2289", 
              "1559-0291"
            ], 
            "name": "Applied Biochemistry and Biotechnology", 
            "publisher": "Springer Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "192"
          }
        ], 
        "keywords": [
          "highest bioconversion rate", 
          "NaNO2 solution", 
          "shake flask", 
          "optimum assay conditions", 
          "rapid screening", 
          "bioconversion rate", 
          "reaction temperature", 
          "detection wavelength", 
          "reaction time", 
          "assay conditions", 
          "environmental samples", 
          "microplates", 
          "microbial isolates", 
          "aryloxyphenoxypropionate herbicides", 
          "wavelength", 
          "hydroxylation activity", 
          "conversion rate", 
          "chromogenic reagent", 
          "solution", 
          "synthesis", 
          "flask", 
          "acid", 
          "method", 
          "microorganisms", 
          "sodium nitrite", 
          "fungi isolates", 
          "single colony", 
          "capacity", 
          "reagents", 
          "microbes", 
          "pH", 
          "samples", 
          "strains", 
          "cultivation", 
          "fungi species", 
          "temperature", 
          "production", 
          "activity", 
          "screening", 
          "rate", 
          "concentration", 
          "nitrite", 
          "min", 
          "hydroxylation", 
          "time", 
          "aid", 
          "results", 
          "conditions", 
          "intermediates", 
          "herbicides", 
          "isolates", 
          "colonies", 
          "A12", 
          "pivotal intermediate", 
          "species", 
          "A5"
        ], 
        "name": "Screening of Fungi Isolates for C-4 Hydroxylation of R-2-Phenoxypropionic Acid Based on a Novel 96-Well Microplate Assay Method", 
        "pagination": "42-56", 
        "productId": [
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1125905011"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/s12010-020-03303-z"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "32212108"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/s12010-020-03303-z", 
          "https://app.dimensions.ai/details/publication/pub.1125905011"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2022-05-20T07:36", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-springernature-scigraph/baseset/20220519/entities/gbq_results/article/article_849.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://doi.org/10.1007/s12010-020-03303-z"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s12010-020-03303-z'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s12010-020-03303-z'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s12010-020-03303-z'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s12010-020-03303-z'


     

    This table displays all metadata directly associated to this object as RDF triples.

    300 TRIPLES      22 PREDICATES      116 URIs      94 LITERALS      27 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/s12010-020-03303-z schema:about N100180d625bb45708890c8ed40185581
    2 N10a80bec996643fd84ea82b9c2a2adad
    3 N12c7d0961a1d44169bd60b97eea6e58c
    4 N12fa1fdb60ca447e9a842341549c68c5
    5 N2b95e1b1f3ff4d889969c5e51bc2effe
    6 N381aab49f22b4b37827c576a7477c5f4
    7 N56c81d60162a419eabaa97919111e62d
    8 N6464e07704bc419abb58bdf548b60b90
    9 N6469659624794f858be82f1f977fe97b
    10 N679580af34de42b5aa1b636234a3eef8
    11 N6f865467a2e54706aa088d773dda857d
    12 N74307a8640b247a3a4725069a641eaed
    13 N8fb75c5739324ab188ce3ecff9aa3d53
    14 Naedcf06d32d343d3965251256ed136f8
    15 Nbdf0e5cbcfc3492883b878c47ed93498
    16 Nc6d972c4996f484f9dade917ff7994ef
    17 Nca5888effd0840e6aabf8ecd2e026399
    18 Nd39fd9e44e7f4ad9bd4ccce8beb20792
    19 Nde2a6c0459a44151ae99ea34ce0f45e3
    20 Ne4b0d11951294d19bc6ae50994abc4cb
    21 anzsrc-for:06
    22 anzsrc-for:0605
    23 schema:author N55df1808a665493e9bfbecf0c7765865
    24 schema:citation sg:pub.10.1007/bf00166241
    25 sg:pub.10.1007/s00253-008-1704-y
    26 sg:pub.10.1007/s00449-019-02154-1
    27 sg:pub.10.1007/s10142-014-0405-0
    28 sg:pub.10.1007/s10295-015-1607-8
    29 sg:pub.10.1007/s10529-009-0171-6
    30 sg:pub.10.1007/s10529-017-2291-8
    31 sg:pub.10.1007/s11356-015-5146-7
    32 sg:pub.10.1007/s12010-013-0570-y
    33 sg:pub.10.1007/s12088-017-0696-7
    34 sg:pub.10.1007/s40899-015-0027-2
    35 sg:pub.10.1038/srep12631
    36 sg:pub.10.1186/s13068-014-0162-2
    37 sg:pub.10.1186/s13068-018-1070-7
    38 schema:datePublished 2020-03-25
    39 schema:datePublishedReg 2020-03-25
    40 schema:description R-2-(4-Hydroxyphenoxy)propionic acid (R-HPPA) is a pivotal intermediate for the synthesis of aryloxyphenoxypropionate (APP) herbicide. To rapidly screen microbial isolates with the capacity of hydroxylating R-2-phenoxypropionic acid to R-HPPA from various environmental samples, a convenient and safe 96-well microplate assay method with sodium nitrite (NaNO2) as chromogenic reagent was proposed and optimized. The optimum assay conditions were as follows: the detection wavelength was 420 nm, the concentration of NaNO2 solution was 6.0 g/L, color reaction temperature was 60 °C, the pH of the NaNO2 solution was 2.4, and the reaction time was 40 min. With the aid of this method, screening for microorganisms with C-4-specific hydroxylation activity of R-PPA was conducted. As a result, 23 strains among 3744 single colonies isolated from various samples exhibited the hydroxylation activity. Among these strains, the highest bioconversion rate was achieved by Penicillium oxalicum A5 and Aspergillus versicolor A12, respectively. After 72-h cultivation in shake flask, their conversion rates of R-HPPA from 10 g/L R-PPA reached 21.18% and 40.24%, respectively. The established method was effective in rapid screening of microbes capable of biosynthesizing R-HPPA through hydroxylation of R-PPA, and the obtained two fungi species could be potentially used for R-HPPA production.
    41 schema:genre article
    42 schema:inLanguage en
    43 schema:isAccessibleForFree false
    44 schema:isPartOf N325ee66a88154132be028525fa694c73
    45 Na828a4100cd64a878e43b87797d237eb
    46 sg:journal.1086169
    47 schema:keywords A12
    48 A5
    49 NaNO2 solution
    50 acid
    51 activity
    52 aid
    53 aryloxyphenoxypropionate herbicides
    54 assay conditions
    55 bioconversion rate
    56 capacity
    57 chromogenic reagent
    58 colonies
    59 concentration
    60 conditions
    61 conversion rate
    62 cultivation
    63 detection wavelength
    64 environmental samples
    65 flask
    66 fungi isolates
    67 fungi species
    68 herbicides
    69 highest bioconversion rate
    70 hydroxylation
    71 hydroxylation activity
    72 intermediates
    73 isolates
    74 method
    75 microbes
    76 microbial isolates
    77 microorganisms
    78 microplates
    79 min
    80 nitrite
    81 optimum assay conditions
    82 pH
    83 pivotal intermediate
    84 production
    85 rapid screening
    86 rate
    87 reaction temperature
    88 reaction time
    89 reagents
    90 results
    91 samples
    92 screening
    93 shake flask
    94 single colony
    95 sodium nitrite
    96 solution
    97 species
    98 strains
    99 synthesis
    100 temperature
    101 time
    102 wavelength
    103 schema:name Screening of Fungi Isolates for C-4 Hydroxylation of R-2-Phenoxypropionic Acid Based on a Novel 96-Well Microplate Assay Method
    104 schema:pagination 42-56
    105 schema:productId N60681a750e894bbfa8e2ff1e46fca36e
    106 Na65a123568c0498a9d4d02d43530bebb
    107 Nfe5e69d4b83a4fc99c3b31f5eb45f87b
    108 schema:sameAs https://app.dimensions.ai/details/publication/pub.1125905011
    109 https://doi.org/10.1007/s12010-020-03303-z
    110 schema:sdDatePublished 2022-05-20T07:36
    111 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    112 schema:sdPublisher N40aa3b8dc8ae40d2bb89230a4542fcc0
    113 schema:url https://doi.org/10.1007/s12010-020-03303-z
    114 sgo:license sg:explorer/license/
    115 sgo:sdDataset articles
    116 rdf:type schema:ScholarlyArticle
    117 N100180d625bb45708890c8ed40185581 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    118 schema:name Catalysis
    119 rdf:type schema:DefinedTerm
    120 N10a80bec996643fd84ea82b9c2a2adad schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    121 schema:name Agar
    122 rdf:type schema:DefinedTerm
    123 N12c7d0961a1d44169bd60b97eea6e58c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    124 schema:name Herbicides
    125 rdf:type schema:DefinedTerm
    126 N12fa1fdb60ca447e9a842341549c68c5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    127 schema:name Phylogeny
    128 rdf:type schema:DefinedTerm
    129 N16b6f1207d01428f89f5dc4d4c9bbc8d rdf:first sg:person.016450272674.19
    130 rdf:rest N71efd9ef3b594c6fa1ca354fc2eae6b6
    131 N2b95e1b1f3ff4d889969c5e51bc2effe schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    132 schema:name Animals
    133 rdf:type schema:DefinedTerm
    134 N325ee66a88154132be028525fa694c73 schema:volumeNumber 192
    135 rdf:type schema:PublicationVolume
    136 N381aab49f22b4b37827c576a7477c5f4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    137 schema:name Sodium Nitrite
    138 rdf:type schema:DefinedTerm
    139 N40aa3b8dc8ae40d2bb89230a4542fcc0 schema:name Springer Nature - SN SciGraph project
    140 rdf:type schema:Organization
    141 N55df1808a665493e9bfbecf0c7765865 rdf:first sg:person.01274560471.40
    142 rdf:rest Nab6b43439e0a4bc186bd7935150e8ab7
    143 N56c81d60162a419eabaa97919111e62d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    144 schema:name Fermentation
    145 rdf:type schema:DefinedTerm
    146 N60681a750e894bbfa8e2ff1e46fca36e schema:name pubmed_id
    147 schema:value 32212108
    148 rdf:type schema:PropertyValue
    149 N6464e07704bc419abb58bdf548b60b90 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    150 schema:name DNA, Ribosomal
    151 rdf:type schema:DefinedTerm
    152 N6469659624794f858be82f1f977fe97b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    153 schema:name Temperature
    154 rdf:type schema:DefinedTerm
    155 N679580af34de42b5aa1b636234a3eef8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    156 schema:name Aspergillus
    157 rdf:type schema:DefinedTerm
    158 N6f865467a2e54706aa088d773dda857d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    159 schema:name Soil Microbiology
    160 rdf:type schema:DefinedTerm
    161 N71efd9ef3b594c6fa1ca354fc2eae6b6 rdf:first sg:person.0613144457.79
    162 rdf:rest N9d2eb30120974dc786f080faec3b8563
    163 N74307a8640b247a3a4725069a641eaed schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    164 schema:name China
    165 rdf:type schema:DefinedTerm
    166 N87295ea7074e410fadfcf97c5324f240 rdf:first sg:person.014132071673.75
    167 rdf:rest N16b6f1207d01428f89f5dc4d4c9bbc8d
    168 N8fb75c5739324ab188ce3ecff9aa3d53 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    169 schema:name Propionates
    170 rdf:type schema:DefinedTerm
    171 N989ca4dd789b4dd09ea9955bce283ff4 rdf:first sg:person.01144225405.06
    172 rdf:rest rdf:nil
    173 N9d2eb30120974dc786f080faec3b8563 rdf:first sg:person.0744724672.04
    174 rdf:rest N989ca4dd789b4dd09ea9955bce283ff4
    175 Na65a123568c0498a9d4d02d43530bebb schema:name dimensions_id
    176 schema:value pub.1125905011
    177 rdf:type schema:PropertyValue
    178 Na828a4100cd64a878e43b87797d237eb schema:issueNumber 1
    179 rdf:type schema:PublicationIssue
    180 Nab6b43439e0a4bc186bd7935150e8ab7 rdf:first sg:person.016322413273.78
    181 rdf:rest N87295ea7074e410fadfcf97c5324f240
    182 Naedcf06d32d343d3965251256ed136f8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    183 schema:name Penicillium
    184 rdf:type schema:DefinedTerm
    185 Nbdf0e5cbcfc3492883b878c47ed93498 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    186 schema:name Hydroxylation
    187 rdf:type schema:DefinedTerm
    188 Nc6d972c4996f484f9dade917ff7994ef schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    189 schema:name Biomass
    190 rdf:type schema:DefinedTerm
    191 Nca5888effd0840e6aabf8ecd2e026399 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    192 schema:name Hydrogen-Ion Concentration
    193 rdf:type schema:DefinedTerm
    194 Nd39fd9e44e7f4ad9bd4ccce8beb20792 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    195 schema:name Bombyx
    196 rdf:type schema:DefinedTerm
    197 Nde2a6c0459a44151ae99ea34ce0f45e3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    198 schema:name Sewage
    199 rdf:type schema:DefinedTerm
    200 Ne4b0d11951294d19bc6ae50994abc4cb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    201 schema:name Phenyl Ethers
    202 rdf:type schema:DefinedTerm
    203 Nfe5e69d4b83a4fc99c3b31f5eb45f87b schema:name doi
    204 schema:value 10.1007/s12010-020-03303-z
    205 rdf:type schema:PropertyValue
    206 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    207 schema:name Biological Sciences
    208 rdf:type schema:DefinedTerm
    209 anzsrc-for:0605 schema:inDefinedTermSet anzsrc-for:
    210 schema:name Microbiology
    211 rdf:type schema:DefinedTerm
    212 sg:grant.8186142 http://pending.schema.org/fundedItem sg:pub.10.1007/s12010-020-03303-z
    213 rdf:type schema:MonetaryGrant
    214 sg:journal.1086169 schema:issn 0273-2289
    215 1559-0291
    216 schema:name Applied Biochemistry and Biotechnology
    217 schema:publisher Springer Nature
    218 rdf:type schema:Periodical
    219 sg:person.01144225405.06 schema:affiliation grid-institutes:grid.469325.f
    220 schema:familyName Zheng
    221 schema:givenName Yu-Guo
    222 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01144225405.06
    223 rdf:type schema:Person
    224 sg:person.01274560471.40 schema:affiliation grid-institutes:grid.469325.f
    225 schema:familyName Zhou
    226 schema:givenName Hai-Yan
    227 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01274560471.40
    228 rdf:type schema:Person
    229 sg:person.014132071673.75 schema:affiliation grid-institutes:grid.469325.f
    230 schema:familyName Li
    231 schema:givenName Yi-Zuo
    232 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014132071673.75
    233 rdf:type schema:Person
    234 sg:person.016322413273.78 schema:affiliation grid-institutes:grid.469325.f
    235 schema:familyName Jiang
    236 schema:givenName Rui
    237 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016322413273.78
    238 rdf:type schema:Person
    239 sg:person.016450272674.19 schema:affiliation grid-institutes:grid.469325.f
    240 schema:familyName Xu
    241 schema:givenName Wei
    242 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016450272674.19
    243 rdf:type schema:Person
    244 sg:person.0613144457.79 schema:affiliation grid-institutes:grid.469325.f
    245 schema:familyName Wang
    246 schema:givenName Yuan-Shan
    247 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0613144457.79
    248 rdf:type schema:Person
    249 sg:person.0744724672.04 schema:affiliation grid-institutes:grid.469325.f
    250 schema:familyName Xue
    251 schema:givenName Ya-Ping
    252 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0744724672.04
    253 rdf:type schema:Person
    254 sg:pub.10.1007/bf00166241 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053066204
    255 https://doi.org/10.1007/bf00166241
    256 rdf:type schema:CreativeWork
    257 sg:pub.10.1007/s00253-008-1704-y schema:sameAs https://app.dimensions.ai/details/publication/pub.1027591760
    258 https://doi.org/10.1007/s00253-008-1704-y
    259 rdf:type schema:CreativeWork
    260 sg:pub.10.1007/s00449-019-02154-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1117065422
    261 https://doi.org/10.1007/s00449-019-02154-1
    262 rdf:type schema:CreativeWork
    263 sg:pub.10.1007/s10142-014-0405-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010985237
    264 https://doi.org/10.1007/s10142-014-0405-0
    265 rdf:type schema:CreativeWork
    266 sg:pub.10.1007/s10295-015-1607-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040653188
    267 https://doi.org/10.1007/s10295-015-1607-8
    268 rdf:type schema:CreativeWork
    269 sg:pub.10.1007/s10529-009-0171-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026074431
    270 https://doi.org/10.1007/s10529-009-0171-6
    271 rdf:type schema:CreativeWork
    272 sg:pub.10.1007/s10529-017-2291-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1083395807
    273 https://doi.org/10.1007/s10529-017-2291-8
    274 rdf:type schema:CreativeWork
    275 sg:pub.10.1007/s11356-015-5146-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012370203
    276 https://doi.org/10.1007/s11356-015-5146-7
    277 rdf:type schema:CreativeWork
    278 sg:pub.10.1007/s12010-013-0570-y schema:sameAs https://app.dimensions.ai/details/publication/pub.1049527472
    279 https://doi.org/10.1007/s12010-013-0570-y
    280 rdf:type schema:CreativeWork
    281 sg:pub.10.1007/s12088-017-0696-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1099701727
    282 https://doi.org/10.1007/s12088-017-0696-7
    283 rdf:type schema:CreativeWork
    284 sg:pub.10.1007/s40899-015-0027-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022121279
    285 https://doi.org/10.1007/s40899-015-0027-2
    286 rdf:type schema:CreativeWork
    287 sg:pub.10.1038/srep12631 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038316224
    288 https://doi.org/10.1038/srep12631
    289 rdf:type schema:CreativeWork
    290 sg:pub.10.1186/s13068-014-0162-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021488829
    291 https://doi.org/10.1186/s13068-014-0162-2
    292 rdf:type schema:CreativeWork
    293 sg:pub.10.1186/s13068-018-1070-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1101533980
    294 https://doi.org/10.1186/s13068-018-1070-7
    295 rdf:type schema:CreativeWork
    296 grid-institutes:grid.469325.f schema:alternateName The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, 18 Chaowang Road, 310014, Hangzhou, People’s Republic of China
    297 schema:name Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, 310014, Hangzhou, People’s Republic of China
    298 Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, People’s Republic of China
    299 The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, 18 Chaowang Road, 310014, Hangzhou, People’s Republic of China
    300 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...