Ontology type: schema:ScholarlyArticle
2017-03-11
AUTHORSLi-Qun Jin, Qi Xu, Zhi-Qiang Liu, Dong-Xu Jia, Cheng-Jun Liao, De-Shui Chen, Yu-Guo Zheng
ABSTRACTGlucose isomerase is the important enzyme for the production of high fructose corn syrup (HFCS). One-step production of HFCS containing more than 55% fructose (HFCS-55) is receiving much attention for its industrial applications. In this work, the Escherichia coli harboring glucose isomerase mutant TEGI-W139F/V186T was immobilized for efficient production of HFCS-55. The immobilization conditions were optimized, and the maximum enzyme activity recovery of 92% was obtained. The immobilized glucose isomerase showed higher pH, temperature, and operational stabilities with a Km value of 272 mM and maximum reaction rate of 23.8 mM min−1. The fructose concentration still retained above 55% after the immobilized glucose isomerase was reused for 10 cycles, and more than 85% of its initial activity was reserved even after 15 recycles of usage at temperature of 90 °C. The results highlighted the immobilized glucose isomerase as a potential biocatalyst for HFCS-55 production. More... »
PAGES293-306
http://scigraph.springernature.com/pub.10.1007/s12010-017-2445-0
DOIhttp://dx.doi.org/10.1007/s12010-017-2445-0
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PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/28285356
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