Production and Characterization of Organic Solvent-Tolerant Cellulase from Bacillus amyloliquefaciens AK9 Isolated from Hot Spring View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2017-01-27

AUTHORS

Muhammad Irfan, Ammara Tayyab, Fariha Hasan, Samiullah Khan, Malik Badshah, Aamer Ali Shah

ABSTRACT

A cellulase-producing bacterium, designated as strain AK9, was isolated from a hot spring of Tatta Pani, Azad Kashmir, Pakistan. The bacterium was identified as Bacillus amyloliquefaciens through 16S rRNA sequencing. Cellulase from strain AK9 was able to liberate glucose from soluble cellulose and carboxymethyl cellulose (CMC). Enzyme was purified through size exclusion chromatography and a single band of ∼47 kDa was observed on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The enzyme was purified with recovery of 35.5%, 3.6-fold purity with specific activity of 31 U mg−1. The purified cellulase retained its activity over a wide range of temperature (50–70 °C) and pH (3–7) with maximum stability at 60 °C and pH 5.0. The activity inhibited by ethylenediaminetetraacetic acid (EDTA), suggested that it was metalloenzyme. Diethyl pyrocarbonate (DEPC) and β-mercaptoethanol significantly inhibited cellulase activity that revealed the essentiality of histidine residues and disulfide bonds for its catalytic function. It was stable in non-ionic surfactants, in the presence of various metal ions, and in water-insoluble organic solvents. Approximately 9.1% of reducing sugar was released after enzymatic saccharification of DAP-pretreated agro-residue, compared to a very low percentage by autohydrolysis treatment. Hence, it is concluded that cellulase from B. amyloliquefaciens AK9 can potentially be used in bioconversion of lignocellulosic biomass to fermentable sugars. More... »

PAGES

1390-1402

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s12010-017-2405-8

DOI

http://dx.doi.org/10.1007/s12010-017-2405-8

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1074237973

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/28130767


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/10", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Technology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Bacillus amyloliquefaciens", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Biomass", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cellulase", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chelating Agents", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Culture Techniques", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Enzyme Stability", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hot Springs", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hydrogen-Ion Concentration", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hydrolysis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Industry", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Metals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Organic Chemicals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Solvents", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Surface-Active Agents", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Temperature", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Department of Microbiology, Faculty of Biological Sciences, Quaid-i-Azam University, 45320, Islamabad, Pakistan", 
          "id": "http://www.grid.ac/institutes/grid.412621.2", 
          "name": [
            "Department of Microbiology, Faculty of Biological Sciences, Quaid-i-Azam University, 45320, Islamabad, Pakistan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Irfan", 
        "givenName": "Muhammad", 
        "id": "sg:person.010505271200.36", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010505271200.36"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Microbiology, Faculty of Biological Sciences, Quaid-i-Azam University, 45320, Islamabad, Pakistan", 
          "id": "http://www.grid.ac/institutes/grid.412621.2", 
          "name": [
            "Department of Microbiology, Faculty of Biological Sciences, Quaid-i-Azam University, 45320, Islamabad, Pakistan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Tayyab", 
        "givenName": "Ammara", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Microbiology, Faculty of Biological Sciences, Quaid-i-Azam University, 45320, Islamabad, Pakistan", 
          "id": "http://www.grid.ac/institutes/grid.412621.2", 
          "name": [
            "Department of Microbiology, Faculty of Biological Sciences, Quaid-i-Azam University, 45320, Islamabad, Pakistan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hasan", 
        "givenName": "Fariha", 
        "id": "sg:person.01164027246.43", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01164027246.43"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Microbiology, Faculty of Biological Sciences, Quaid-i-Azam University, 45320, Islamabad, Pakistan", 
          "id": "http://www.grid.ac/institutes/grid.412621.2", 
          "name": [
            "Department of Microbiology, Faculty of Biological Sciences, Quaid-i-Azam University, 45320, Islamabad, Pakistan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Khan", 
        "givenName": "Samiullah", 
        "id": "sg:person.013240112057.04", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013240112057.04"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Microbiology, Faculty of Biological Sciences, Quaid-i-Azam University, 45320, Islamabad, Pakistan", 
          "id": "http://www.grid.ac/institutes/grid.412621.2", 
          "name": [
            "Department of Microbiology, Faculty of Biological Sciences, Quaid-i-Azam University, 45320, Islamabad, Pakistan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Badshah", 
        "givenName": "Malik", 
        "id": "sg:person.01010345126.01", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01010345126.01"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Microbiology, Faculty of Biological Sciences, Quaid-i-Azam University, 45320, Islamabad, Pakistan", 
          "id": "http://www.grid.ac/institutes/grid.412621.2", 
          "name": [
            "Department of Microbiology, Faculty of Biological Sciences, Quaid-i-Azam University, 45320, Islamabad, Pakistan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Shah", 
        "givenName": "Aamer Ali", 
        "id": "sg:person.0714504125.51", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0714504125.51"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/s12010-010-9132-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015536203", 
          "https://doi.org/10.1007/s12010-010-9132-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/s12896-015-0129-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034866729", 
          "https://doi.org/10.1186/s12896-015-0129-9"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00792-011-0403-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035983591", 
          "https://doi.org/10.1007/s00792-011-0403-2"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10924-013-0600-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041216238", 
          "https://doi.org/10.1007/s10924-013-0600-4"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/227680a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010419937", 
          "https://doi.org/10.1038/227680a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00792-012-0463-y", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006413990", 
          "https://doi.org/10.1007/s00792-012-0463-y"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10295-009-0528-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050496871", 
          "https://doi.org/10.1007/s10295-009-0528-9"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2017-01-27", 
    "datePublishedReg": "2017-01-27", 
    "description": "A cellulase-producing bacterium, designated as strain AK9, was isolated from a hot spring of Tatta Pani, Azad Kashmir, Pakistan. The bacterium was identified as Bacillus amyloliquefaciens through 16S rRNA sequencing. Cellulase from strain AK9 was able to liberate glucose from soluble cellulose and carboxymethyl cellulose (CMC). Enzyme was purified through size exclusion chromatography and a single band of \u223c47\u00a0kDa was observed on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The enzyme was purified with recovery of 35.5%, 3.6-fold purity with specific activity of 31\u00a0U\u00a0mg\u22121. The purified cellulase retained its activity over a wide range of temperature (50\u201370\u00a0\u00b0C) and pH (3\u20137) with maximum stability at 60\u00a0\u00b0C and pH\u00a05.0. The activity inhibited by ethylenediaminetetraacetic acid (EDTA), suggested that it was metalloenzyme. Diethyl pyrocarbonate (DEPC) and \u03b2-mercaptoethanol significantly inhibited cellulase activity that revealed the essentiality of histidine residues and disulfide bonds for its catalytic function. It was stable in non-ionic surfactants, in the presence of various metal ions, and in water-insoluble organic solvents. Approximately 9.1% of reducing sugar was released after enzymatic saccharification of DAP-pretreated agro-residue, compared to a very low percentage by autohydrolysis treatment. Hence, it is concluded that cellulase from B. amyloliquefaciens AK9 can potentially be used in bioconversion of lignocellulosic biomass to fermentable sugars.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/s12010-017-2405-8", 
    "inLanguage": "en", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1086169", 
        "issn": [
          "0273-2289", 
          "1559-0291"
        ], 
        "name": "Applied Biochemistry and Biotechnology", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "4", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "182"
      }
    ], 
    "keywords": [
      "water-insoluble organic solvents", 
      "non-ionic surfactants", 
      "carboxymethyl cellulose", 
      "size exclusion chromatography", 
      "ethylenediaminetetraacetic acid", 
      "metal ions", 
      "organic solvents", 
      "sodium dodecyl sulfate-polyacrylamide gel electrophoresis", 
      "exclusion chromatography", 
      "maximum stability", 
      "soluble cellulose", 
      "catalytic function", 
      "dodecyl sulfate-polyacrylamide gel electrophoresis", 
      "sulfate-polyacrylamide gel electrophoresis", 
      "lignocellulosic biomass", 
      "autohydrolysis treatment", 
      "Tatta Pani", 
      "cellulose", 
      "PANI", 
      "histidine residues", 
      "solvent", 
      "surfactants", 
      "bonds", 
      "ions", 
      "diethyl", 
      "chromatography", 
      "single band", 
      "gel electrophoresis", 
      "purity", 
      "wide range", 
      "characterization", 
      "stability", 
      "agro-residues", 
      "acid", 
      "specific activity", 
      "mercaptoethanol", 
      "temperature", 
      "AK9", 
      "sugars", 
      "cellulase-producing bacterium", 
      "enzymatic saccharification", 
      "electrophoresis", 
      "band", 
      "cellulase", 
      "enzyme", 
      "activity", 
      "residues", 
      "range", 
      "presence", 
      "recovery", 
      "bioconversion", 
      "fermentable sugars", 
      "DAP", 
      "hot springs", 
      "biomass", 
      "glucose", 
      "production", 
      "cellulase activity", 
      "saccharification", 
      "essentiality", 
      "bacterium", 
      "lower percentage", 
      "treatment", 
      "percentage", 
      "function", 
      "rRNA sequencing", 
      "spring", 
      "bacilli", 
      "Azad Kashmir", 
      "sequencing", 
      "Pakistan", 
      "Kashmir", 
      "strain AK9", 
      "Organic Solvent-Tolerant Cellulase", 
      "Solvent-Tolerant Cellulase"
    ], 
    "name": "Production and Characterization of Organic Solvent-Tolerant Cellulase from Bacillus amyloliquefaciens AK9 Isolated from Hot Spring", 
    "pagination": "1390-1402", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1074237973"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s12010-017-2405-8"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "28130767"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s12010-017-2405-8", 
      "https://app.dimensions.ai/details/publication/pub.1074237973"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2021-12-01T19:39", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20211201/entities/gbq_results/article/article_739.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/s12010-017-2405-8"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s12010-017-2405-8'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s12010-017-2405-8'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s12010-017-2405-8'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s12010-017-2405-8'


 

This table displays all metadata directly associated to this object as RDF triples.

259 TRIPLES      22 PREDICATES      123 URIs      108 LITERALS      22 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s12010-017-2405-8 schema:about N104c12d9d9a34f20b0622ad7cfe5f0b4
2 N17e2421646a8407a9da5d2ef45689800
3 N23c3997482fa48e0afc86689fc7cd1ed
4 N361af2d098d1487ca53798daec0a81ac
5 N4723538f60184eaca42b85e2fc3b34cf
6 N52626def39d24afc9c01e1ddd35b9b49
7 N64fe5fdfa4d34856b618f509313bfcff
8 N655ec57bbbbf4cd0a9836c67f4a307b4
9 N6a4cf51ed4c24b2289c7e7a2bae6da16
10 N8e08a10b889a4b41965ce167b6207ab8
11 N9f312b84b0f840d3bf6d27375ace1f40
12 Na8960c1a1dee4eaf9ee92463fc61bb32
13 Nb03c20dabf044deea4f23f6033a54259
14 Nc586cd93c317483c9b7deac084f9af32
15 Ncda81ad0d35c4075966fc149bf91d078
16 anzsrc-for:06
17 anzsrc-for:10
18 schema:author Nadcc31effa054f3b8a038c579c2d0e9a
19 schema:citation sg:pub.10.1007/s00792-011-0403-2
20 sg:pub.10.1007/s00792-012-0463-y
21 sg:pub.10.1007/s10295-009-0528-9
22 sg:pub.10.1007/s10924-013-0600-4
23 sg:pub.10.1007/s12010-010-9132-8
24 sg:pub.10.1038/227680a0
25 sg:pub.10.1186/s12896-015-0129-9
26 schema:datePublished 2017-01-27
27 schema:datePublishedReg 2017-01-27
28 schema:description A cellulase-producing bacterium, designated as strain AK9, was isolated from a hot spring of Tatta Pani, Azad Kashmir, Pakistan. The bacterium was identified as Bacillus amyloliquefaciens through 16S rRNA sequencing. Cellulase from strain AK9 was able to liberate glucose from soluble cellulose and carboxymethyl cellulose (CMC). Enzyme was purified through size exclusion chromatography and a single band of ∼47 kDa was observed on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The enzyme was purified with recovery of 35.5%, 3.6-fold purity with specific activity of 31 U mg−1. The purified cellulase retained its activity over a wide range of temperature (50–70 °C) and pH (3–7) with maximum stability at 60 °C and pH 5.0. The activity inhibited by ethylenediaminetetraacetic acid (EDTA), suggested that it was metalloenzyme. Diethyl pyrocarbonate (DEPC) and β-mercaptoethanol significantly inhibited cellulase activity that revealed the essentiality of histidine residues and disulfide bonds for its catalytic function. It was stable in non-ionic surfactants, in the presence of various metal ions, and in water-insoluble organic solvents. Approximately 9.1% of reducing sugar was released after enzymatic saccharification of DAP-pretreated agro-residue, compared to a very low percentage by autohydrolysis treatment. Hence, it is concluded that cellulase from B. amyloliquefaciens AK9 can potentially be used in bioconversion of lignocellulosic biomass to fermentable sugars.
29 schema:genre article
30 schema:inLanguage en
31 schema:isAccessibleForFree false
32 schema:isPartOf Nd297b41428764d32ab0b0f0aac116f4a
33 Nf8a6daf80b74487086f46b869694a55c
34 sg:journal.1086169
35 schema:keywords AK9
36 Azad Kashmir
37 DAP
38 Kashmir
39 Organic Solvent-Tolerant Cellulase
40 PANI
41 Pakistan
42 Solvent-Tolerant Cellulase
43 Tatta Pani
44 acid
45 activity
46 agro-residues
47 autohydrolysis treatment
48 bacilli
49 bacterium
50 band
51 bioconversion
52 biomass
53 bonds
54 carboxymethyl cellulose
55 catalytic function
56 cellulase
57 cellulase activity
58 cellulase-producing bacterium
59 cellulose
60 characterization
61 chromatography
62 diethyl
63 dodecyl sulfate-polyacrylamide gel electrophoresis
64 electrophoresis
65 enzymatic saccharification
66 enzyme
67 essentiality
68 ethylenediaminetetraacetic acid
69 exclusion chromatography
70 fermentable sugars
71 function
72 gel electrophoresis
73 glucose
74 histidine residues
75 hot springs
76 ions
77 lignocellulosic biomass
78 lower percentage
79 maximum stability
80 mercaptoethanol
81 metal ions
82 non-ionic surfactants
83 organic solvents
84 percentage
85 presence
86 production
87 purity
88 rRNA sequencing
89 range
90 recovery
91 residues
92 saccharification
93 sequencing
94 single band
95 size exclusion chromatography
96 sodium dodecyl sulfate-polyacrylamide gel electrophoresis
97 soluble cellulose
98 solvent
99 specific activity
100 spring
101 stability
102 strain AK9
103 sugars
104 sulfate-polyacrylamide gel electrophoresis
105 surfactants
106 temperature
107 treatment
108 water-insoluble organic solvents
109 wide range
110 schema:name Production and Characterization of Organic Solvent-Tolerant Cellulase from Bacillus amyloliquefaciens AK9 Isolated from Hot Spring
111 schema:pagination 1390-1402
112 schema:productId N0561e4d6b05d4a4dbccdd2db0049344e
113 N2448db9b46af4ccb8a3c7435483b9185
114 Nb6a2be5e8b6c461cb88bf7aaf85a30bd
115 schema:sameAs https://app.dimensions.ai/details/publication/pub.1074237973
116 https://doi.org/10.1007/s12010-017-2405-8
117 schema:sdDatePublished 2021-12-01T19:39
118 schema:sdLicense https://scigraph.springernature.com/explorer/license/
119 schema:sdPublisher Na9bde62abd244b5d8404753df3d2c237
120 schema:url https://doi.org/10.1007/s12010-017-2405-8
121 sgo:license sg:explorer/license/
122 sgo:sdDataset articles
123 rdf:type schema:ScholarlyArticle
124 N0561e4d6b05d4a4dbccdd2db0049344e schema:name pubmed_id
125 schema:value 28130767
126 rdf:type schema:PropertyValue
127 N104c12d9d9a34f20b0622ad7cfe5f0b4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
128 schema:name Hydrogen-Ion Concentration
129 rdf:type schema:DefinedTerm
130 N17e2421646a8407a9da5d2ef45689800 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
131 schema:name Chelating Agents
132 rdf:type schema:DefinedTerm
133 N23c3997482fa48e0afc86689fc7cd1ed schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
134 schema:name Cellulase
135 rdf:type schema:DefinedTerm
136 N2448db9b46af4ccb8a3c7435483b9185 schema:name dimensions_id
137 schema:value pub.1074237973
138 rdf:type schema:PropertyValue
139 N361af2d098d1487ca53798daec0a81ac schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
140 schema:name Temperature
141 rdf:type schema:DefinedTerm
142 N4723538f60184eaca42b85e2fc3b34cf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
143 schema:name Enzyme Stability
144 rdf:type schema:DefinedTerm
145 N52626def39d24afc9c01e1ddd35b9b49 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
146 schema:name Metals
147 rdf:type schema:DefinedTerm
148 N63b4755f62f444189fab48f43812acfc rdf:first sg:person.013240112057.04
149 rdf:rest Nc94db7403c094a3990a351ebf2de1611
150 N64fe5fdfa4d34856b618f509313bfcff schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
151 schema:name Industry
152 rdf:type schema:DefinedTerm
153 N655ec57bbbbf4cd0a9836c67f4a307b4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
154 schema:name Biomass
155 rdf:type schema:DefinedTerm
156 N6a4cf51ed4c24b2289c7e7a2bae6da16 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
157 schema:name Bacillus amyloliquefaciens
158 rdf:type schema:DefinedTerm
159 N8e08a10b889a4b41965ce167b6207ab8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
160 schema:name Surface-Active Agents
161 rdf:type schema:DefinedTerm
162 N9f312b84b0f840d3bf6d27375ace1f40 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
163 schema:name Solvents
164 rdf:type schema:DefinedTerm
165 Na7d61c05ceb64a48a8b88c4639b2c15e schema:affiliation grid-institutes:grid.412621.2
166 schema:familyName Tayyab
167 schema:givenName Ammara
168 rdf:type schema:Person
169 Na8960c1a1dee4eaf9ee92463fc61bb32 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
170 schema:name Hydrolysis
171 rdf:type schema:DefinedTerm
172 Na9bde62abd244b5d8404753df3d2c237 schema:name Springer Nature - SN SciGraph project
173 rdf:type schema:Organization
174 Nadcc31effa054f3b8a038c579c2d0e9a rdf:first sg:person.010505271200.36
175 rdf:rest Nf8b55657590645f1b781d27277f03abd
176 Nb03c20dabf044deea4f23f6033a54259 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
177 schema:name Organic Chemicals
178 rdf:type schema:DefinedTerm
179 Nb6a2be5e8b6c461cb88bf7aaf85a30bd schema:name doi
180 schema:value 10.1007/s12010-017-2405-8
181 rdf:type schema:PropertyValue
182 Nc586cd93c317483c9b7deac084f9af32 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
183 schema:name Hot Springs
184 rdf:type schema:DefinedTerm
185 Nc94db7403c094a3990a351ebf2de1611 rdf:first sg:person.01010345126.01
186 rdf:rest Ne666154634c140e9b94abbe4755add1d
187 Ncda81ad0d35c4075966fc149bf91d078 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
188 schema:name Culture Techniques
189 rdf:type schema:DefinedTerm
190 Nd297b41428764d32ab0b0f0aac116f4a schema:volumeNumber 182
191 rdf:type schema:PublicationVolume
192 Ne666154634c140e9b94abbe4755add1d rdf:first sg:person.0714504125.51
193 rdf:rest rdf:nil
194 Ne97a5f2a8736442abddf9824e506c049 rdf:first sg:person.01164027246.43
195 rdf:rest N63b4755f62f444189fab48f43812acfc
196 Nf8a6daf80b74487086f46b869694a55c schema:issueNumber 4
197 rdf:type schema:PublicationIssue
198 Nf8b55657590645f1b781d27277f03abd rdf:first Na7d61c05ceb64a48a8b88c4639b2c15e
199 rdf:rest Ne97a5f2a8736442abddf9824e506c049
200 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
201 schema:name Biological Sciences
202 rdf:type schema:DefinedTerm
203 anzsrc-for:10 schema:inDefinedTermSet anzsrc-for:
204 schema:name Technology
205 rdf:type schema:DefinedTerm
206 sg:journal.1086169 schema:issn 0273-2289
207 1559-0291
208 schema:name Applied Biochemistry and Biotechnology
209 schema:publisher Springer Nature
210 rdf:type schema:Periodical
211 sg:person.01010345126.01 schema:affiliation grid-institutes:grid.412621.2
212 schema:familyName Badshah
213 schema:givenName Malik
214 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01010345126.01
215 rdf:type schema:Person
216 sg:person.010505271200.36 schema:affiliation grid-institutes:grid.412621.2
217 schema:familyName Irfan
218 schema:givenName Muhammad
219 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010505271200.36
220 rdf:type schema:Person
221 sg:person.01164027246.43 schema:affiliation grid-institutes:grid.412621.2
222 schema:familyName Hasan
223 schema:givenName Fariha
224 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01164027246.43
225 rdf:type schema:Person
226 sg:person.013240112057.04 schema:affiliation grid-institutes:grid.412621.2
227 schema:familyName Khan
228 schema:givenName Samiullah
229 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013240112057.04
230 rdf:type schema:Person
231 sg:person.0714504125.51 schema:affiliation grid-institutes:grid.412621.2
232 schema:familyName Shah
233 schema:givenName Aamer Ali
234 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0714504125.51
235 rdf:type schema:Person
236 sg:pub.10.1007/s00792-011-0403-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035983591
237 https://doi.org/10.1007/s00792-011-0403-2
238 rdf:type schema:CreativeWork
239 sg:pub.10.1007/s00792-012-0463-y schema:sameAs https://app.dimensions.ai/details/publication/pub.1006413990
240 https://doi.org/10.1007/s00792-012-0463-y
241 rdf:type schema:CreativeWork
242 sg:pub.10.1007/s10295-009-0528-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050496871
243 https://doi.org/10.1007/s10295-009-0528-9
244 rdf:type schema:CreativeWork
245 sg:pub.10.1007/s10924-013-0600-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041216238
246 https://doi.org/10.1007/s10924-013-0600-4
247 rdf:type schema:CreativeWork
248 sg:pub.10.1007/s12010-010-9132-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015536203
249 https://doi.org/10.1007/s12010-010-9132-8
250 rdf:type schema:CreativeWork
251 sg:pub.10.1038/227680a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010419937
252 https://doi.org/10.1038/227680a0
253 rdf:type schema:CreativeWork
254 sg:pub.10.1186/s12896-015-0129-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034866729
255 https://doi.org/10.1186/s12896-015-0129-9
256 rdf:type schema:CreativeWork
257 grid-institutes:grid.412621.2 schema:alternateName Department of Microbiology, Faculty of Biological Sciences, Quaid-i-Azam University, 45320, Islamabad, Pakistan
258 schema:name Department of Microbiology, Faculty of Biological Sciences, Quaid-i-Azam University, 45320, Islamabad, Pakistan
259 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...