Production of Xylitol from d-Xylose by Overexpression of Xylose Reductase in Osmotolerant Yeast Candida glycerinogenes WL2002-5 View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2015-07

AUTHORS

Cheng Zhang, Hong Zong, Bin Zhuge, Xinyao Lu, Huiying Fang, Jian Zhuge

ABSTRACT

Efficient bioconversion of D-xylose into various biochemicals is critical for the developing lignocelluloses application. In this study, we compared D-xylose utilization in Candida glycerinogenes WL2002-5 transformants expressing xylose reductase (XYL1) in D-xylose metabolism. C. glycerinogenes WL2002-5 expressing XYL1 from Schefferomyces stipitis can produce xylitol. Xylitol production by the recombinant strains was evaluated using a xylitol fermentation medium with glucose as a co-substrate. As glucose was found to be an insufficient co-substrate, various carbon sources were screened for efficient cofactor regeneration, and glycerol was found to be the best co-substrate. The effects of glycerol on the xylitol production rate by a xylose reductase gene (XYL1)-overexpressed mutant of C. glycerinogenes WL2002-5 were investigated. The XYL1-overexpressed mutant produced xylitol from D-xylose using glycerol as a co-substrate for cell growth and NAD (P) H regeneration: 100 g/L D-xylose was completely converted into xylitol when at least 20 g/L glycerol was used as a co-substrate. XYL1 overexpressed mutant grown on glycerol as co-substrate accumulated 2.1-fold increased xylitol concentration over those cells grown on glucose as co-substrate. XYL1 overexpressed mutant produced xylitol with a volumetric productivity of 0.83 g/L/h, and a xylitol yield of 98 % xylose. Recombinant yeast strains obtained in this study are promising candidates for xylitol production. This is the first report of XYL1 gene overexpression of C. glycerinogenes WL2002-5 for enhancing the efficiency of xylitol production. More... »

PAGES

1511-1527

References to SciGraph publications

  • 2003-12. Real-time PCR based on SYBR-Green I fluorescence: An alternative to the TaqMan assay for a relative quantification of gene rearrangements, gene amplifications and micro gene deletions in BMC BIOTECHNOLOGY
  • 2009-05. Promotional mechanism of high glycerol productivity in the aerobic batch fermentation of Candida glycerinogenes after feeding several amino acids in APPLIED BIOCHEMISTRY AND MICROBIOLOGY
  • 1970-08. Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 in NATURE
  • 2015-01. Integrative expression vectors for overexpression of xylitol dehydrogenase (XYL2) in Osmotolerant yeast, Candida glycerinogenes WL2002-5 in JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
  • 2012-12. Improved ethanol production by a xylose-fermenting recombinant yeast strain constructed through a modified genome shuffling method in BIOTECHNOLOGY FOR BIOFUELS
  • 2004-05. Metabolic engineering for improved fermentation of pentoses by yeasts in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2003-02. By-product formation by a novel glycerol-producing yeast, Candida glycerinogenes, with different O2 supplies in BIOTECHNOLOGY LETTERS
  • 2010-04. Xylitol production by recombinant Corynebacterium glutamicum under oxygen deprivation in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 1974-12. Regulatory properties of the constitutive hexose transport in saccharomyces cerevisiae in MOLECULAR AND CELLULAR BIOCHEMISTRY
  • 2006-08. Enhancement of xylitol productivity and yield using a xylitol dehydrogenase gene-disrupted mutant of Candida tropicalis under fully aerobic conditions in BIOTECHNOLOGY LETTERS
  • 2007-12. Statistical tools for transgene copy number estimation based on real-time PCR in BMC BIOINFORMATICS
  • 1967-03. Properties of the sugar carrier in baker's yeast in FOLIA MICROBIOLOGICA
  • 2012-10. Metabolic engineering of Bacillus subtilis for enhanced production of acetoin in BIOTECHNOLOGY LETTERS
  • 2001-06. Glycerol production by a novel osmotolerant yeast Candida glycerinogenes in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2009-08. Ethanol production from xylose in engineered Saccharomyces cerevisiae strains: current state and perspectives in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2009-12. Ethanol and xylitol production from glucose and xylose at high temperature by Kluyveromyces sp. IIPE453 in JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s12010-015-1661-8

    DOI

    http://dx.doi.org/10.1007/s12010-015-1661-8

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1029152906

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/26018342


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