Purification and Characterization of a Cysteine-Rich 14-kDa Antibacterial Peptide from the Granular Hemocytes of Mangrove Crab Episesarma tetragonum and Its ... View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2015-06-10

AUTHORS

Jeyachandran Sivakamavalli, Raju Nirosha, Baskaralingam Vaseeharan

ABSTRACT

Antimicrobial peptide (AMP) crustin is a type of immune molecule present in the immune system of crustaceans and response against microbial invasion. In the present study, we have identified and characterized the cationic, amphipathic structure consisting of AMP crustin from a mangrove crab Episesarma tetragonum using CM Sepharose-based cation exchange column chromatography. E. tetragonum crustin showed a single band of 14 kDa on SDS-PAGE and the homogeneity showed retention time of 8.4 min in RP-HPLC. Functional studies of E. tetragonum crustin exhibits the antibacterial activity (2–4 μg/ml) and biofilm inhibition (20 μg/ml) against the Gram-positive bacteria Staphylococcus aureus and Enterococcus faecalis. Hydrophobicity and extrapolysaccharide production of Gram-positive bacteria were inhibited through the bactericidal inhibitory concentration. In situ visualization analysis of biofilm inhibition was observed through light and confocal laser scanning microscopy. Surface morphology and the bacterial biofilm inhibition were viewed by scanning electron and atomic force microscopy. This study emphasizes the potential activity of E. tetragonum crustin, an interesting candidate for the development of novel broad-spectrum antimicrobial agent against bacterial pathogens.Graphical AbstractAntimicrobial peptide synthesis and host-pathogen interaction lead to production of immune molecules directed to destruction of pathogens. More... »

PAGES

1084-1101

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s12010-015-1631-1

DOI

http://dx.doi.org/10.1007/s12010-015-1631-1

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1025216716

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/26059067


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