Synthesis and Characterization of Reduced Graphene Oxide Supported Gold Nanoparticles-Poly(Pyrrole-Co-Pyrrolepropylic Acid) Nanocomposite-Based Electrochemical Biosensor View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2014-06-14

AUTHORS

Nidhi Puri, Asad Niazi, Avanish K. Srivastava, Rajesh

ABSTRACT

A conducting poly(pyrrole-co-pyrrolepropylic acid) copolymer nanocomposite film (AuNP-PPy-PPa) incorporating gold nanoparticles (AuNP) was electrochemically grown using a single step procedure over electrochemically reduced graphene oxide (RGO) flakes deposited on a silane-modified indium-tin-oxide (ITO) glass plate. The RGO support base provided excellent mechanical and chemical stability to the polymer nanocomposite matrix. The porous nanostructure of AuNP-PPy-PPa/RGO provided a huge accessible area to disperse AuNP, and it avoided metallic agglomeration within the polymer matrix. The AuNP-PPy-PPa/RGO was characterized by high-resolution transmission electron microscopy (HRTEM), contact angle measurements, Fourier transform infrared spectroscopy (FTIR), and electrochemical techniques. The pendant carboxyl group of AuNP-PPy-PPa/RGO was covalently bonded with myoglobin protein antibody, Ab-Mb, for the construction of a bioelectrode. Electrochemical impedance spectroscopy technique was used for the characterization of the bioelectrode and as an impedimetric biosensor for the detection of human cardiac biomarker, Ag-cMb. The bioelectrode exhibited a linear impedimetric response to Ag-cMb in the range of 10 ng mL−1 to 1 μg mL−1, in phosphate-buffered solution (PBS) (pH 7.4, 0.1 M KCl) with a sensitivity of 92.13 Ω cm2 per decade. More... »

PAGES

911-925

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s12010-014-0997-9

DOI

http://dx.doi.org/10.1007/s12010-014-0997-9

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1046697622

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24928550


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