Ontology type: schema:ScholarlyArticle
2014-03
AUTHORSG. Bleve, C. Lezzi, S. Spagnolo, P. Rampino, C. Perrotta, G. Mita, Francesco Grieco
ABSTRACTThe ERY4 laccase gene from Pleurotus eryngii was expressed in Saccharomyces cerevisiae and the recombinant laccase resulted to be not biologically active. This gene was thus modified to obtain chimerical enzymes derived from the substitution of N-, C- and both N- and C-terminal regions with the corresponding regions of Ery3 laccase, another laccase isoform of P. eryngii. The chimerical isoform named 4NC3, derived from the substitution of both N- and C-terminal regions, showed the best performances in terms of enzymatic activities, affinities for different substrates and stability at a broad range of temperatures and pHs. The chimerical 4NC3 laccase isoform was displayed on the cell surface of S. cerevisiae using the N-terminal fusion with either the Pir2 or the Flo1 S. cerevisiae proteins as anchor attachment sequence. Immunofluorescence microscopy and Western blot analyses confirmed the localization of 4NC3 on the yeast cell surface. The enzyme activity on specific laccase substrates revealed that 4NC3 laccase was immobilized in active form on the cell surface. To our knowledge, this is the first example of expression of a chimerical fungal laccase by yeast cell display. More... »
PAGES2916-2931
http://scigraph.springernature.com/pub.10.1007/s12010-014-0734-4
DOIhttp://dx.doi.org/10.1007/s12010-014-0734-4
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PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/24458655
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},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"24458655"
]
},
{
"name": "nlm_unique_id",
"type": "PropertyValue",
"value": [
"8208561"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1007/s12010-014-0734-4"
]
},
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
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]
}
],
"sameAs": [
"https://doi.org/10.1007/s12010-014-0734-4",
"https://app.dimensions.ai/details/publication/pub.1018241628"
],
"sdDataset": "articles",
"sdDatePublished": "2019-04-10T23:26",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8693_00000521.jsonl",
"type": "ScholarlyArticle",
"url": "http://link.springer.com/10.1007%2Fs12010-014-0734-4"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s12010-014-0734-4'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s12010-014-0734-4'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s12010-014-0734-4'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s12010-014-0734-4'
This table displays all metadata directly associated to this object as RDF triples.
329 TRIPLES
21 PREDICATES
91 URIs
36 LITERALS
24 BLANK NODES