Thidiazuron-Induced Changes in Biomass Parameters, Total Phenolic Content, and Antioxidant Activity in Callus Cultures of Artemisia absinthium L. View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2014-03

AUTHORS

Mohammad Ali, Bilal Haider Abbasi

ABSTRACT

Callus culture of Artemisia absinthium L. was established for enhanced production of phenolics and higher antioxidant activity. Callus was induced from seed-derived leaf explants, incubated on to MS media supplemented with thidiazuron (TDZ; 0.5-5.0 mg/l) either alone or in combination with α-naphthalene acetic acid (NAA; 1.0 mg/l). These callus cultures were investigated for their growth kinetics, total phenolic content, and antioxidant activity on weekly basis for a period of 49 days. Maximum dry biomass accumulation of 8.73 g/l was observed on day 42 in response to 1.0 mg/l TDZ and 1.0 mg/l NAA. Furthermore, maximum level of total phenolic content of 8.53 mg GAE/g DW and highest 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity of 72.6% were observed in calli formed in response to 1.0 mg/l TDZ on day 42. The results showed a positive correlation of total phenolic content and DPPH radical scavenging activity in most of the callus cultures of A. absinthium L. More... »

PAGES

2363-2376

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s12010-013-0663-7

DOI

http://dx.doi.org/10.1007/s12010-013-0663-7

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1038933392

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24371002


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