Isolation, Purification and Characterisation of an Organic Solvent-Tolerant Ca2+-Dependent Protease from Bacillus megaterium AU02 View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2014-01

AUTHORS

J Deepa Arul Priya, K Divakar, M Suryia Prabha, G Panneer Selvam, Pennathur Gautam

ABSTRACT

A new organic solvent-tolerant strain Bacillus megaterium AU02 which secretes an organic solvent-tolerant protease was isolated from milk industry waste. Statistical methods were employed to achieve optimum protease production of 43.6 U/ml in shake flask cultures. The productivity of the protease was increased to 53 U/ml when cultivated under controlled conditions in a 7-L fermentor. The protease was purified to homogeneity by a three-step process with 24 % yield and specific activity of 5,375 U/mg. The molecular mass of the protease was found to be 59 kDa. The enzyme was active over a wide range of pH (6.0–9.0), with an optimum activity at pH 7.0 and temperature from 40 to 70 °C having an optimum activity at 50 °C. The thermal stability of the enzyme increased significantly in the presence of CaCl2, and it retained 90 % activity at 50 °C for 3 h. The Km and Vmax values were determined as 0.722 mg/ml and 0.018 U/mg respectively. The metalloprotease exhibited significant stability in the presence of organic solvents with log P values more than 2.5, nonionic detergents and oxidising agent. An attempt was made to test the synthesis of aspartame precursor (Cbz-Asp-Phe-NH2) which was catalysed by AU02 protease in the presence of 50 % DMSO. These properties of AU02 protease make it an ideal choice for enzymatic peptide synthesis in organic media. More... »

PAGES

910-932

References to SciGraph publications

  • 1995-03. Inactivation of the major extracellular protease from Bacillus megaterium DSM319 by gene replacement in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2010-10. An organic solvent-, detergent-, and thermostable alkaline protease from the mesophilic, organic solvent-tolerant Bacillus licheniformis 3C5 in MICROBIOLOGY
  • 2002-04. Optimization of Alkaline Protease Production from Bacillus sp. by Response Surface Methodology in CURRENT MICROBIOLOGY
  • 2010-02. Achievements and perspectives to overcome the poor solvent resistance in acetone and butanol-producing microorganisms in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2002-12. An overview on fermentation, downstream processing and properties of microbial alkaline proteases in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2003-12. Purification and partial characterization of thermostable serine alkaline protease from a newly isolatedBacillus subtilis PE-11 in AAPS PHARMSCITECH
  • 2001-01. Improving enzymes by using them in organic solvents in NATURE
  • 2008-06. Purification and characterization of organic solvent stable protease from Bacillus licheniformis RSP-09-37 in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2011-06. Purification and partial characterization of a detergent and oxidizing agent stable alkaline protease from a newly isolated Bacillus subtilis VSG-4 of tropical soil in JOURNAL OF MICROBIOLOGY
  • 1985-07. A continuous culture study of the bioenergetic aspects of growth and production of exocellular protease in Bacillus licheniformis in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2005-10. Microbial proteases in peptide synthesis: approaches and applications in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2002-06. Bacterial alkaline proteases: molecular approaches and industrial applications in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 1970-08. Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 in NATURE
  • 1984-12. Polyethylene glycol-modified enzymes trap water on their surface and exert enzymic activity in organic solvents in BIOTECHNOLOGY LETTERS
  • 2002-08. Biodegradation of high-concentration isopropanol by a solvent-tolerant thermophile, Bacillus pallidus in EXTREMOPHILES
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s12010-013-0589-0

    DOI

    http://dx.doi.org/10.1007/s12010-013-0589-0

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1044333070

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/24122712


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