Identification and Functional Characterization of an α-Amylase with Broad Temperature and pH Stability from Paenibacillus sp. View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2013-05

AUTHORS

Thangamani Rajesh, Yong Hyun Kim, Yong-Keun Choi, Jong Min Jeon, Hyun Joong Kim, Sung-Hee Park, Hyung-Yeon Park, Kwon-Young Choi, Hyungsup Kim, Hyung Joo Kim, Sang Hyun Lee, Yung-Hun Yang

ABSTRACT

Amylases are important industrial enzymes that have been applied widely in the food, detergent, and pulp industries and fermentation processes. In the present study, a gene encoding an alpha-amylase from the genomic DNA library of Paenibacillus sp. was identified and characterized. The amylase gene designated amy1 was shown to consist of 1,980 bp and shared sequence identity towards α-amylase genes from other Bacillus sp. The deduced amino acid sequence for Amy1 indicated 80 % sequence identity with other Bacillus strains. Heterologous expression of recombinant Amy1 in Escherichia coli BL21(DE3) facilitated the recovery of this protein in soluble form. Enzyme kinetic data revealed Amy1 to have a K m of 23.83 mg/mL and K cat of 48.74 min(-1) and K cat /K m of 2 min(-1) mg(-1) mL(-1) for starch. The activity of this protein was found to be enhanced by Mn(2+), and furthermore, Amy1 remained active at a broad pH range (4-10) and temperature (30-90 °C). The ability of Amy1 to act on food waste under broad temperature and pH conditions, together with its ability to produce simple sugars, shows many advantages for further application in the food industry. More... »

PAGES

359-369

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s12010-013-0197-z

DOI

http://dx.doi.org/10.1007/s12010-013-0197-z

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1034683316

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/23526111


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Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s12010-013-0197-z'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s12010-013-0197-z'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s12010-013-0197-z'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s12010-013-0197-z'


 

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