Xylitol Production by Genetically Engineered Trichoderma reesei Strains Using Barley Straw as Feedstock View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2013-01

AUTHORS

Mehdi Dashtban, Greg Kepka, Bernhard Seiboth, Wensheng Qin

ABSTRACT

Xylitol, a naturally occurring five-carbon sugar alcohol derived from D-xylose, is currently in high demand by industries. Trichoderma reesei, a prolific industrial cellulase and hemicellulase producing fungus, is able to selectively use D-xylose from hemicelluloses for xylitol production. The xylitol production by T. reesei can be enhanced by genetic engineering of blocking further xylitol metabolism in the D-xylose pathway. We have used two different T. reesei strains which are impaired in the further metabolism of xylitol including a single mutant in which the xylitol dehydrogenase gene was deleted (∆xdh1) and a double mutant where additionally L-arabinitol-4-dehydrogenase, an enzyme which can partially compensate for xylitol dehydrogenase function, was deleted (∆lad1∆xdh1). Barely straw was first pretreated using NaOH and Organosolv pretreatment methods. The highest xylitol production of 6.1 and 13.22 g/L was obtained using medium supplemented with 2 % Organosolv-pretreated barley straw and 2 % D-xylose by the ∆xdh1 and ∆lad1∆xdh1 strains, respectively. More... »

PAGES

554-569

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s12010-012-0008-y

DOI

http://dx.doi.org/10.1007/s12010-012-0008-y

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1017643432

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/23247825


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