Improvement of l-Arginine Production by Overexpression of a Bifunctional Ornithine Acetyltransferase in Corynebacterium crenatum View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2011-10

AUTHORS

Wenfang Dou, Meijuan Xu, Dongmei Cai, Xiaomei Zhang, Zhiming Rao, Zhenghong Xu

ABSTRACT

Ornithine acetyltransferase (EC 2.3.1.35; OATase) gene (argJ) from the L-arginine-producing mutant Corynebacterium crenatum SYPA5-5 was cloned, sequenced, and expressed in Escherichia coli BL21 (DE3). Analysis of the argJ sequence revealed that the argJ coded a polypeptide of 388 amino acids with a calculated molecular weight of 39.7 kDa. In this study, the function of the OATase (argJ) of C. crenatum SYPA5-5 has been identified as a conserved ATML sequence for the autolysis of the protein to α- and β-subunits. When the argJ regions corresponding to the α- and β-subunits were cloned and expressed separately in E. coli BL21, OATase activities were abolished. At the same time, a functional study revealed that OATase from C. crenatum SYPA5-5 was a bifunctional enzyme with the functions of acetylglutamate synthase (EC 2.3.1.1, NAGS) and acetylornithine deacetylase (EC 3.5.1.16, AOase) activities. In order to investigate the effects of the overexpression of the argJ gene on L: -arginine production, the argJ gene was inserted into pJCtac to yield the recombinant shuttle plasmid pJCtac-argJ and then transformed into C. crenatum SYPA5-5. The results showed that the engineered strains could not only express more OATase (90.9%) but also increase the production of L: -arginine significantly (16.8%). More... »

PAGES

845-855

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s12010-011-9302-3

DOI

http://dx.doi.org/10.1007/s12010-011-9302-3

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1030888483

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/21785983


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Acetyltransferases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Amino Acid Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Arginine", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Bacterial Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cloning, Molecular", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Corynebacterium", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Escherichia coli", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Industrial Microbiology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Sequence Data", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Plasmids", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Subunits", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sequence Homology, Amino Acid", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transformation, Bacterial", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Jiangnan University", 
          "id": "https://www.grid.ac/institutes/grid.258151.a", 
          "name": [
            "Laboratory of Pharmaceutical Engineering, School of Medicine and Pharmaceutics, Jiangnan University, 214122, Wuxi, People\u2019s Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Dou", 
        "givenName": "Wenfang", 
        "id": "sg:person.0753433256.99", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0753433256.99"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Jiangnan University", 
          "id": "https://www.grid.ac/institutes/grid.258151.a", 
          "name": [
            "The Key Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, 214122, Wuxi, Jiangsu Province, People\u2019s Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Xu", 
        "givenName": "Meijuan", 
        "id": "sg:person.0637204656.41", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0637204656.41"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Jiangnan University", 
          "id": "https://www.grid.ac/institutes/grid.258151.a", 
          "name": [
            "Laboratory of Pharmaceutical Engineering, School of Medicine and Pharmaceutics, Jiangnan University, 214122, Wuxi, People\u2019s Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Cai", 
        "givenName": "Dongmei", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Jiangnan University", 
          "id": "https://www.grid.ac/institutes/grid.258151.a", 
          "name": [
            "Laboratory of Pharmaceutical Engineering, School of Medicine and Pharmaceutics, Jiangnan University, 214122, Wuxi, People\u2019s Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zhang", 
        "givenName": "Xiaomei", 
        "id": "sg:person.0610231311.32", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0610231311.32"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Jiangnan University", 
          "id": "https://www.grid.ac/institutes/grid.258151.a", 
          "name": [
            "The Key Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, 214122, Wuxi, Jiangsu Province, People\u2019s Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Rao", 
        "givenName": "Zhiming", 
        "id": "sg:person.015323230632.97", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015323230632.97"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Jiangnan University", 
          "id": "https://www.grid.ac/institutes/grid.258151.a", 
          "name": [
            "Laboratory of Pharmaceutical Engineering, School of Medicine and Pharmaceutics, Jiangnan University, 214122, Wuxi, People\u2019s Republic of China", 
            "The Key Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, 214122, Wuxi, Jiangsu Province, People\u2019s Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Xu", 
        "givenName": "Zhenghong", 
        "id": "sg:person.01021546456.51", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01021546456.51"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1111/j.1432-1033.1997.0232a.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003273698"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m100392200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004583111"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.c500005200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004618675"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/3-540-45989-8_2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007701674", 
          "https://doi.org/10.1007/3-540-45989-8_2"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/3-540-45989-8_2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007701674", 
          "https://doi.org/10.1007/3-540-45989-8_2"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1432-1327.2000.01593.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016823486"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.275.15.11361", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016860619"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s002530051557", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018538662", 
          "https://doi.org/10.1007/s002530051557"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mmbr.00032-06", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022373228"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bej.2008.08.007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030486969"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-3-540-48596-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035408004", 
          "https://doi.org/10.1007/978-3-540-48596-4"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-3-540-48596-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035408004", 
          "https://doi.org/10.1007/978-3-540-48596-4"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s12010-010-9076-z", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039256156", 
          "https://doi.org/10.1007/s12010-010-9076-z"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-003-1328-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040642222", 
          "https://doi.org/10.1007/s00253-003-1328-1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0168-1656(03)00148-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040999160"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0168-1656(03)00148-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040999160"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10295-010-0760-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045776358", 
          "https://doi.org/10.1007/s10295-010-0760-3"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10295-010-0760-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045776358", 
          "https://doi.org/10.1007/s10295-010-0760-3"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jb.01831-07", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045875917"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-005-0308-z", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053287313", 
          "https://doi.org/10.1007/s00253-005-0308-z"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-005-0308-z", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053287313", 
          "https://doi.org/10.1007/s00253-005-0308-z"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bst0320310", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053476059"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bst0320310", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053476059"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi982574a", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055216971"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi982574a", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055216971"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1099/00221287-144-2-479", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060369767"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1099/13500872-140-8-1817", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060380534"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1099/13500872-142-1-99", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060380996"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jb.174.8.2694-2701.1992", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062721489"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074319214", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079806771", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083254935", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083352626", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2011-10", 
    "datePublishedReg": "2011-10-01", 
    "description": "Ornithine acetyltransferase (EC 2.3.1.35; OATase) gene (argJ) from the L-arginine-producing mutant Corynebacterium crenatum SYPA5-5 was cloned, sequenced, and expressed in Escherichia coli BL21 (DE3). Analysis of the argJ sequence revealed that the argJ coded a polypeptide of 388 amino acids with a calculated molecular weight of 39.7 kDa. In this study, the function of the OATase (argJ) of C. crenatum SYPA5-5 has been identified as a conserved ATML sequence for the autolysis of the protein to \u03b1- and \u03b2-subunits. When the argJ regions corresponding to the \u03b1- and \u03b2-subunits were cloned and expressed separately in E. coli BL21, OATase activities were abolished. At the same time, a functional study revealed that OATase from C. crenatum SYPA5-5 was a bifunctional enzyme with the functions of acetylglutamate synthase (EC 2.3.1.1, NAGS) and acetylornithine deacetylase (EC 3.5.1.16, AOase) activities. In order to investigate the effects of the overexpression of the argJ gene on L: -arginine production, the argJ gene was inserted into pJCtac to yield the recombinant shuttle plasmid pJCtac-argJ and then transformed into C. crenatum SYPA5-5. The results showed that the engineered strains could not only express more OATase (90.9%) but also increase the production of L: -arginine significantly (16.8%).", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s12010-011-9302-3", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1086169", 
        "issn": [
          "0273-2289", 
          "1559-0291"
        ], 
        "name": "Applied Biochemistry and Biotechnology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "3-4", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "165"
      }
    ], 
    "name": "Improvement of l-Arginine Production by Overexpression of a Bifunctional Ornithine Acetyltransferase in Corynebacterium crenatum", 
    "pagination": "845-855", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "9326086187c63ef84f929428813767285f1422bb16c8c21eac889293d16d1f90"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "21785983"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "8208561"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s12010-011-9302-3"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1030888483"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s12010-011-9302-3", 
      "https://app.dimensions.ai/details/publication/pub.1030888483"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T16:44", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8669_00000522.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007%2Fs12010-011-9302-3"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s12010-011-9302-3'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s12010-011-9302-3'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s12010-011-9302-3'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s12010-011-9302-3'


 

This table displays all metadata directly associated to this object as RDF triples.

241 TRIPLES      21 PREDICATES      69 URIs      35 LITERALS      23 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s12010-011-9302-3 schema:about N03fbc033957345579196770a1a1e3819
2 N1ae608d533b54d80a0efda2a4e08069c
3 N22bd3549af9d448e986575f68a6b4e6e
4 N23187613d1af476797da733e43831f0c
5 N35849c9165b84335abbde24647727692
6 N68c17d4acf824bd3aae5addbda33d7aa
7 N7d3d18f5eadd418a95fd9cc8beeccd69
8 N8462f4b09f7246ff9c41deb77e21f160
9 N85076309b2244621925ce76b6477d214
10 Na03937a3a7fb4a5090c76df8d4fb7135
11 Nb01874523ae644149930e0ccb486ec5c
12 Nc050e194aa7e41709bcb35fa63effcca
13 Nc9b60d5691784bc48d7cd8f7d3bd3768
14 Nde891cde7cc24cfbb37c6b6c77eedb8b
15 anzsrc-for:06
16 anzsrc-for:0604
17 schema:author N03359a11d6b14230aed68f3fc4fdb433
18 schema:citation sg:pub.10.1007/3-540-45989-8_2
19 sg:pub.10.1007/978-3-540-48596-4
20 sg:pub.10.1007/s00253-003-1328-1
21 sg:pub.10.1007/s00253-005-0308-z
22 sg:pub.10.1007/s002530051557
23 sg:pub.10.1007/s10295-010-0760-3
24 sg:pub.10.1007/s12010-010-9076-z
25 https://app.dimensions.ai/details/publication/pub.1074319214
26 https://app.dimensions.ai/details/publication/pub.1079806771
27 https://app.dimensions.ai/details/publication/pub.1083254935
28 https://app.dimensions.ai/details/publication/pub.1083352626
29 https://doi.org/10.1016/j.bej.2008.08.007
30 https://doi.org/10.1016/s0168-1656(03)00148-2
31 https://doi.org/10.1021/bi982574a
32 https://doi.org/10.1042/bst0320310
33 https://doi.org/10.1046/j.1432-1327.2000.01593.x
34 https://doi.org/10.1074/jbc.275.15.11361
35 https://doi.org/10.1074/jbc.c500005200
36 https://doi.org/10.1074/jbc.m100392200
37 https://doi.org/10.1099/00221287-144-2-479
38 https://doi.org/10.1099/13500872-140-8-1817
39 https://doi.org/10.1099/13500872-142-1-99
40 https://doi.org/10.1111/j.1432-1033.1997.0232a.x
41 https://doi.org/10.1128/jb.01831-07
42 https://doi.org/10.1128/jb.174.8.2694-2701.1992
43 https://doi.org/10.1128/mmbr.00032-06
44 schema:datePublished 2011-10
45 schema:datePublishedReg 2011-10-01
46 schema:description Ornithine acetyltransferase (EC 2.3.1.35; OATase) gene (argJ) from the L-arginine-producing mutant Corynebacterium crenatum SYPA5-5 was cloned, sequenced, and expressed in Escherichia coli BL21 (DE3). Analysis of the argJ sequence revealed that the argJ coded a polypeptide of 388 amino acids with a calculated molecular weight of 39.7 kDa. In this study, the function of the OATase (argJ) of C. crenatum SYPA5-5 has been identified as a conserved ATML sequence for the autolysis of the protein to α- and β-subunits. When the argJ regions corresponding to the α- and β-subunits were cloned and expressed separately in E. coli BL21, OATase activities were abolished. At the same time, a functional study revealed that OATase from C. crenatum SYPA5-5 was a bifunctional enzyme with the functions of acetylglutamate synthase (EC 2.3.1.1, NAGS) and acetylornithine deacetylase (EC 3.5.1.16, AOase) activities. In order to investigate the effects of the overexpression of the argJ gene on L: -arginine production, the argJ gene was inserted into pJCtac to yield the recombinant shuttle plasmid pJCtac-argJ and then transformed into C. crenatum SYPA5-5. The results showed that the engineered strains could not only express more OATase (90.9%) but also increase the production of L: -arginine significantly (16.8%).
47 schema:genre research_article
48 schema:inLanguage en
49 schema:isAccessibleForFree false
50 schema:isPartOf Nc6a2e4f438fe4de0b0ebde80bf87a084
51 Ne609296f461f412cb9c4657594042828
52 sg:journal.1086169
53 schema:name Improvement of l-Arginine Production by Overexpression of a Bifunctional Ornithine Acetyltransferase in Corynebacterium crenatum
54 schema:pagination 845-855
55 schema:productId N1c0a4a8d24634d01a2a9056e517bf7f7
56 N5e629c7ffdd94bac8bad8c208bded5ba
57 N7592842c6ce84f0cb70e8286504cfab7
58 Na176733354654955906628622f4baa40
59 Na192e14cf75f405a9b8fecf55494e95e
60 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030888483
61 https://doi.org/10.1007/s12010-011-9302-3
62 schema:sdDatePublished 2019-04-10T16:44
63 schema:sdLicense https://scigraph.springernature.com/explorer/license/
64 schema:sdPublisher N5746a6a64ce24e189b53870ff493551f
65 schema:url http://link.springer.com/10.1007%2Fs12010-011-9302-3
66 sgo:license sg:explorer/license/
67 sgo:sdDataset articles
68 rdf:type schema:ScholarlyArticle
69 N03359a11d6b14230aed68f3fc4fdb433 rdf:first sg:person.0753433256.99
70 rdf:rest N1eb65b4730304013b710773dd9aa73a4
71 N03fbc033957345579196770a1a1e3819 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
72 schema:name Plasmids
73 rdf:type schema:DefinedTerm
74 N1ae608d533b54d80a0efda2a4e08069c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
75 schema:name Arginine
76 rdf:type schema:DefinedTerm
77 N1c0a4a8d24634d01a2a9056e517bf7f7 schema:name readcube_id
78 schema:value 9326086187c63ef84f929428813767285f1422bb16c8c21eac889293d16d1f90
79 rdf:type schema:PropertyValue
80 N1eb65b4730304013b710773dd9aa73a4 rdf:first sg:person.0637204656.41
81 rdf:rest Nbc7a5605aaf54f0880f7467f5f245927
82 N22bd3549af9d448e986575f68a6b4e6e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
83 schema:name Acetyltransferases
84 rdf:type schema:DefinedTerm
85 N23187613d1af476797da733e43831f0c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
86 schema:name Bacterial Proteins
87 rdf:type schema:DefinedTerm
88 N34fbcdc7d55e471aa59367c5f09b4bb9 rdf:first sg:person.015323230632.97
89 rdf:rest Nb21adffbf2dc44c28339197a96d10818
90 N35849c9165b84335abbde24647727692 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
91 schema:name Protein Subunits
92 rdf:type schema:DefinedTerm
93 N5746a6a64ce24e189b53870ff493551f schema:name Springer Nature - SN SciGraph project
94 rdf:type schema:Organization
95 N5e629c7ffdd94bac8bad8c208bded5ba schema:name nlm_unique_id
96 schema:value 8208561
97 rdf:type schema:PropertyValue
98 N68c17d4acf824bd3aae5addbda33d7aa schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
99 schema:name Cloning, Molecular
100 rdf:type schema:DefinedTerm
101 N7592842c6ce84f0cb70e8286504cfab7 schema:name doi
102 schema:value 10.1007/s12010-011-9302-3
103 rdf:type schema:PropertyValue
104 N7d3d18f5eadd418a95fd9cc8beeccd69 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
105 schema:name Molecular Sequence Data
106 rdf:type schema:DefinedTerm
107 N8462f4b09f7246ff9c41deb77e21f160 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
108 schema:name Transformation, Bacterial
109 rdf:type schema:DefinedTerm
110 N85076309b2244621925ce76b6477d214 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
111 schema:name Escherichia coli
112 rdf:type schema:DefinedTerm
113 Na03937a3a7fb4a5090c76df8d4fb7135 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
114 schema:name Amino Acid Sequence
115 rdf:type schema:DefinedTerm
116 Na176733354654955906628622f4baa40 schema:name dimensions_id
117 schema:value pub.1030888483
118 rdf:type schema:PropertyValue
119 Na192e14cf75f405a9b8fecf55494e95e schema:name pubmed_id
120 schema:value 21785983
121 rdf:type schema:PropertyValue
122 Na7a0fab422d4463d9838eb1a509c48f4 rdf:first sg:person.0610231311.32
123 rdf:rest N34fbcdc7d55e471aa59367c5f09b4bb9
124 Nb01874523ae644149930e0ccb486ec5c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
125 schema:name Industrial Microbiology
126 rdf:type schema:DefinedTerm
127 Nb21adffbf2dc44c28339197a96d10818 rdf:first sg:person.01021546456.51
128 rdf:rest rdf:nil
129 Nbc7a5605aaf54f0880f7467f5f245927 rdf:first Nc7d14183198644e3bcacb5e202279f21
130 rdf:rest Na7a0fab422d4463d9838eb1a509c48f4
131 Nc050e194aa7e41709bcb35fa63effcca schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
132 schema:name Sequence Homology, Amino Acid
133 rdf:type schema:DefinedTerm
134 Nc6a2e4f438fe4de0b0ebde80bf87a084 schema:volumeNumber 165
135 rdf:type schema:PublicationVolume
136 Nc7d14183198644e3bcacb5e202279f21 schema:affiliation https://www.grid.ac/institutes/grid.258151.a
137 schema:familyName Cai
138 schema:givenName Dongmei
139 rdf:type schema:Person
140 Nc9b60d5691784bc48d7cd8f7d3bd3768 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
141 schema:name Corynebacterium
142 rdf:type schema:DefinedTerm
143 Nde891cde7cc24cfbb37c6b6c77eedb8b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
144 schema:name Gene Expression
145 rdf:type schema:DefinedTerm
146 Ne609296f461f412cb9c4657594042828 schema:issueNumber 3-4
147 rdf:type schema:PublicationIssue
148 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
149 schema:name Biological Sciences
150 rdf:type schema:DefinedTerm
151 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
152 schema:name Genetics
153 rdf:type schema:DefinedTerm
154 sg:journal.1086169 schema:issn 0273-2289
155 1559-0291
156 schema:name Applied Biochemistry and Biotechnology
157 rdf:type schema:Periodical
158 sg:person.01021546456.51 schema:affiliation https://www.grid.ac/institutes/grid.258151.a
159 schema:familyName Xu
160 schema:givenName Zhenghong
161 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01021546456.51
162 rdf:type schema:Person
163 sg:person.015323230632.97 schema:affiliation https://www.grid.ac/institutes/grid.258151.a
164 schema:familyName Rao
165 schema:givenName Zhiming
166 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015323230632.97
167 rdf:type schema:Person
168 sg:person.0610231311.32 schema:affiliation https://www.grid.ac/institutes/grid.258151.a
169 schema:familyName Zhang
170 schema:givenName Xiaomei
171 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0610231311.32
172 rdf:type schema:Person
173 sg:person.0637204656.41 schema:affiliation https://www.grid.ac/institutes/grid.258151.a
174 schema:familyName Xu
175 schema:givenName Meijuan
176 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0637204656.41
177 rdf:type schema:Person
178 sg:person.0753433256.99 schema:affiliation https://www.grid.ac/institutes/grid.258151.a
179 schema:familyName Dou
180 schema:givenName Wenfang
181 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0753433256.99
182 rdf:type schema:Person
183 sg:pub.10.1007/3-540-45989-8_2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007701674
184 https://doi.org/10.1007/3-540-45989-8_2
185 rdf:type schema:CreativeWork
186 sg:pub.10.1007/978-3-540-48596-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035408004
187 https://doi.org/10.1007/978-3-540-48596-4
188 rdf:type schema:CreativeWork
189 sg:pub.10.1007/s00253-003-1328-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040642222
190 https://doi.org/10.1007/s00253-003-1328-1
191 rdf:type schema:CreativeWork
192 sg:pub.10.1007/s00253-005-0308-z schema:sameAs https://app.dimensions.ai/details/publication/pub.1053287313
193 https://doi.org/10.1007/s00253-005-0308-z
194 rdf:type schema:CreativeWork
195 sg:pub.10.1007/s002530051557 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018538662
196 https://doi.org/10.1007/s002530051557
197 rdf:type schema:CreativeWork
198 sg:pub.10.1007/s10295-010-0760-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045776358
199 https://doi.org/10.1007/s10295-010-0760-3
200 rdf:type schema:CreativeWork
201 sg:pub.10.1007/s12010-010-9076-z schema:sameAs https://app.dimensions.ai/details/publication/pub.1039256156
202 https://doi.org/10.1007/s12010-010-9076-z
203 rdf:type schema:CreativeWork
204 https://app.dimensions.ai/details/publication/pub.1074319214 schema:CreativeWork
205 https://app.dimensions.ai/details/publication/pub.1079806771 schema:CreativeWork
206 https://app.dimensions.ai/details/publication/pub.1083254935 schema:CreativeWork
207 https://app.dimensions.ai/details/publication/pub.1083352626 schema:CreativeWork
208 https://doi.org/10.1016/j.bej.2008.08.007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030486969
209 rdf:type schema:CreativeWork
210 https://doi.org/10.1016/s0168-1656(03)00148-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040999160
211 rdf:type schema:CreativeWork
212 https://doi.org/10.1021/bi982574a schema:sameAs https://app.dimensions.ai/details/publication/pub.1055216971
213 rdf:type schema:CreativeWork
214 https://doi.org/10.1042/bst0320310 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053476059
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1046/j.1432-1327.2000.01593.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1016823486
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1074/jbc.275.15.11361 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016860619
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1074/jbc.c500005200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004618675
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1074/jbc.m100392200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004583111
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1099/00221287-144-2-479 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060369767
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1099/13500872-140-8-1817 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060380534
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1099/13500872-142-1-99 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060380996
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1111/j.1432-1033.1997.0232a.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1003273698
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1128/jb.01831-07 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045875917
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1128/jb.174.8.2694-2701.1992 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062721489
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1128/mmbr.00032-06 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022373228
237 rdf:type schema:CreativeWork
238 https://www.grid.ac/institutes/grid.258151.a schema:alternateName Jiangnan University
239 schema:name Laboratory of Pharmaceutical Engineering, School of Medicine and Pharmaceutics, Jiangnan University, 214122, Wuxi, People’s Republic of China
240 The Key Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, 214122, Wuxi, Jiangsu Province, People’s Republic of China
241 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...