Pilot-Scale Fermentation of Aqueous-Ammonia-Soaked Switchgrass View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2009-06

AUTHORS

Asli Isci, Jennifer N. Himmelsbach, John Strohl, Anthony L. Pometto, D. Raj Raman, Robert P. Anex

ABSTRACT

Aqueous-ammonia-steeped switchgrass was subject to simultaneous saccharification and fermentation (SSF) in two pilot-scale bioreactors (50- and 350-L working volume). Switchgrass was pretreated by soaking in ammonium hydroxide (30%) with solid to liquid ratio of 5 L ammonium hydroxide per kilogram dry switchgrass for 5 days in 75-L steeping vessels without agitation at ambient temperatures (15 to 33 degrees C). SSF of the pretreated biomass was carried out using Saccharomyces cerevisiae (D(5)A) at approximately 2% glucan and 77 filter paper units per gram cellulose enzyme loading (Spezyme CP). The 50-L fermentation was carried out aseptically, whereas the 350-L fermentation was semiaseptic. The percentage of maximum theoretical ethanol yields achieved was 73% in the 50-L reactor and 52-74% in the 350-L reactor due to the difference in asepsis. The 350-L fermentation was contaminated by acid-producing bacteria (lactic and acetic acid concentrations approaching 10 g/L), and this resulted in lower ethanol production. Despite this problem, the pilot-scale SSF of aqueous-ammonia-pretreated switchgrass has shown promising results similar to laboratory-scale experiments. This work demonstrates challenges in pilot-scale fermentations with material handling, aseptic conditions, and bacterial contamination for cellulosic fermentations to biofuels. More... »

PAGES

453

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s12010-008-8235-y

DOI

http://dx.doi.org/10.1007/s12010-008-8235-y

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1022819871

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/18716923


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