Aqueous Ammonia Soaking of Switchgrass Followed by Simultaneous Saccharification and Fermentation View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2008-01

AUTHORS

Asli Isci, Jennifer N. Himmelsbach, Anthony L. Pometto, D. Raj Raman, Robert P. Anex

ABSTRACT

Simultaneous saccharification and fermentation (SSF) of switchgrass was performed following aqueous ammonia pretreatment. Switchgrass was soaked in aqueous ammonium hydroxide (30%) with different liquid-solid ratios (5 and 10 ml/g) for either 5 or 10 days. The pretreatment was carried out at atmospheric conditions without agitation. A 40-50% delignification (Klason lignin basis) was achieved, whereas cellulose content remained unchanged and hemicellulose content decreased by approximately 50%. The Sacccharomyces cerevisiae (D5A)-mediated SSF of ammonia-treated switchgrass was investigated at two glucan loadings (3 and 6%) and three enzyme loadings (26, 38.5, and 77 FPU/g cellulose), using Spezyme CP. The percentage of maximum theoretical ethanol yield achieved was 72. Liquid-solid ratio and steeping time affected lignin removal slightly, but did not cause a significant change in overall ethanol conversion yields at sufficiently high enzyme loadings. These results suggest that ammonia steeping may be an effective method of pretreatment for lignocellulosic feedstocks. More... »

PAGES

69-77

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s12010-007-8008-z

DOI

http://dx.doi.org/10.1007/s12010-007-8008-z

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1036163478

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/18415988


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