Establishment of a high-frequency plant regeneration system from rhizome-derived embryogenic cell-suspension cultures of Curcuma longa L. View Full Text


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Article Info

DATE

2019-02-07

AUTHORS

Eun Yee Jie, Myung Suk Ahn, Jiyoung Lee, Ye In Cheon, Cha Young Kim, Suk Weon Kim

ABSTRACT

We developed an efficient plant regeneration technique for turmeric (Curcuma longa L.) via somatic embryogenesis derived from embryogenic cell suspensions cultured from petioles. We initiated in vitro callus cultures from the plantlets of turmeric cultured on Murashige and Skoog (MS) medium. Then leaf, petiole, stem, and root explants of in vitro-grown plantlets were cultured on Schenk and Hildebrandt (SH). The stem and root explants formed white nodular calluses at a frequency of 86.7% and 85.8%, respectively, when cultured on SH medium; however, we used the calluses derived from the root explants for initiating a cell-suspension culture. In this study, the actively growing cell suspension culture comprised small, highly cytoplasmic cell aggregates and large vacuolated cells. On being transferred to a basal SH medium (without growth regulators), a few of these calluses differentiated into somatic embryos, which indicated that the initial calluses were embryogenic cells. We stimulated additional shoot differentiation by adding 0.5 mg L−1N-phenyl-N′-1,2,3-thiadiazol-5-yl urea (TDZ) and various concentrations of N6-benzyladenine (BA) to the embryogenic cell suspension. The highest frequency at which shoot primordia developed from embryogenic cell clusters was 70% (when cultured in the dark on the supplemented MS medium), with more than 83.3% of regenerated shoot primordia producing roots. We successfully transplanted rooted plantlets into a soil mixture of sterile vermiculite and potting soil (1:1) and grew them to maturity in a growth chamber, achieving a survival rate of > 95%. To our knowledge, this is the first report of complete plant regeneration of turmeric from embryogenic cell-suspension cultures. The embryogenic cell line and plant regeneration system established in this study can be applied to mass proliferation and gene manipulation for improving turmeric quality. More... »

PAGES

1-7

References to SciGraph publications

  • 1990-01. In vitro clonal multiplication of turmeric (Curcuma spp.) and ginger (Zingiber officinale Rosc.) in PLANT CELL REPORTS
  • 2003-07. High-frequency shoot multiplication in Curcuma longa L. using thidiazuron in PLANT CELL REPORTS
  • 2011-02. Evaluation of phytomedicinal yield potential and molecular profiling of micropropagated and conventionally grown turmeric (Curcuma longa L.) in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 2008-12. Plant regeneration from callus culture of Curcuma aromatica and in vitro detection of somaclonal variation through cytophotometric analysis in BIOLOGIA PLANTARUM
  • 2004-03. In vitro Plant Regeneration and Genotype Conservation of Eight Wild Species of Curcuma in BIOLOGIA PLANTARUM
  • 2005-09. In vitro regeneration of Hagenia abyssinica (Bruce) J.F. Gmel. (Rosaceae) from leaf explants in PLANT CELL REPORTS
  • 2003-04. Plant regeneration of rose (Rosa hybridia) from embryogenic cell-derived protoplasts in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 2001-08. Plant regeneration from leaf base callus of turmeric and random amplified polymorphic DNA analysis of regenerated plants in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1998-10. Thidiazuron: A potent regulator ofin vitro plant morphogenesis in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY - PLANT
  • 2008. Ginger and Turmeric Ancient Spices and Modern Medicines in GENOMICS OF TROPICAL CROP PLANTS
  • 2014-03. Somatic embryogenesis and Agrobacterium-mediated transformation of turmeric (Curcuma longa) in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 2010-09. Plant regeneration in Curcuma species and assessment of genetic stability of regenerated plants in BIOLOGIA PLANTARUM
  • 2002-02. Micropropagation and field evaluation of micropropagated plants of turmeric in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 2006-03. An efficient protocol for genetic transformation and shoot regeneration of turmeric (Curcuma longa L.) via particle bombardment in PLANT CELL REPORTS
  • 2001-01. Factors affecting in vitro microrhizome production in turmeric in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 2000-09. Direct regeneration of shoots from immature inflorescence cultures of turmeric in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
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    33 schema:description We developed an efficient plant regeneration technique for turmeric (Curcuma longa L.) via somatic embryogenesis derived from embryogenic cell suspensions cultured from petioles. We initiated in vitro callus cultures from the plantlets of turmeric cultured on Murashige and Skoog (MS) medium. Then leaf, petiole, stem, and root explants of in vitro-grown plantlets were cultured on Schenk and Hildebrandt (SH). The stem and root explants formed white nodular calluses at a frequency of 86.7% and 85.8%, respectively, when cultured on SH medium; however, we used the calluses derived from the root explants for initiating a cell-suspension culture. In this study, the actively growing cell suspension culture comprised small, highly cytoplasmic cell aggregates and large vacuolated cells. On being transferred to a basal SH medium (without growth regulators), a few of these calluses differentiated into somatic embryos, which indicated that the initial calluses were embryogenic cells. We stimulated additional shoot differentiation by adding 0.5 mg L−1N-phenyl-N′-1,2,3-thiadiazol-5-yl urea (TDZ) and various concentrations of N6-benzyladenine (BA) to the embryogenic cell suspension. The highest frequency at which shoot primordia developed from embryogenic cell clusters was 70% (when cultured in the dark on the supplemented MS medium), with more than 83.3% of regenerated shoot primordia producing roots. We successfully transplanted rooted plantlets into a soil mixture of sterile vermiculite and potting soil (1:1) and grew them to maturity in a growth chamber, achieving a survival rate of > 95%. To our knowledge, this is the first report of complete plant regeneration of turmeric from embryogenic cell-suspension cultures. The embryogenic cell line and plant regeneration system established in this study can be applied to mass proliferation and gene manipulation for improving turmeric quality.
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