Silver-ion high-performance liquid chromatographic separation and identification of conjugated linoleic acid isomers View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1998-02

AUTHORS

Najibullah Sehat, Martin P. Yurawecz, John A. G. Roach, Magdi M. Mossoba, John K. G. Kramer, Youh Ku

ABSTRACT

This is the first report of the application of silver-ion impregnated high-performance liquid chromatography (Ag(+)-HPLC) to the separation of complex mixtures of conjugated linolenic acid (CLA) isomers present in commercial CLA sources and foods and in biological specimens. This method showed a clear separation of CLA isomers into three groups related to their trans,trans, cis,trans or trans,cis, and cic,cis configuration of the conjugated double-bond system. In addition, this method separated individual positional isomers of the conjugated diene system within each geometrical isomeric group. Following Ag(+)-HPLC isolation, gas chromatography (GC)-electron impact mass spectrometry, and GC-direct deposition-Fourier transformed infrared spectroscopy were used to confirm the identity of two major positional isomers in the cis/trans region, i.e., delta 8,10- and delta 11,13-octadecadienoic acids, which had not been chromatographically resolved previously. Furthermore, the potential of this method was demonstrated by showing different Ag(+)-HPLC profiles exhibiting patterns of isomeric distributions for biological specimens from animals fed a diet containing a commercial CLA preparation, as well as for a commercial cheese product. More... »

PAGES

217-221

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s11745-998-0198-6

DOI

http://dx.doi.org/10.1007/s11745-998-0198-6

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1037367052

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/9507244


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