Phospholipid identification and quantification of membrane vesicle subfractions by 31P−1H two-dimensional nuclear magnetic resonance View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2000-11

AUTHORS

B. Larijani, D. L. Poccia, L. Charles Dickinson

ABSTRACT

An approach to the direct quantification of phospholipids from two-dimensional 31P-1H nuclear magnetic resonance (NMR) spectroscopy with isotropic proton mixing has been developed as a general method for phospholipid analysis of minor membrane vesicle subfractions. Membrane vesicles were subfractionated by sedimentation to density equilibrium in a sucrose gradient, and a modified Folch method was employed to extract their phospholipids. The coefficient for the NMR detection efficiency of each phospholipid and the relative mole percentage of the phospholipids present in the membrane vesicles were calculated. We demonstrate low detection limits such that relative concentrations of phospholipids in membrane subfractions may be determined even in the submicromolar range. More... »

PAGES

1289-1297

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s11745-000-0645-4

DOI

http://dx.doi.org/10.1007/s11745-000-0645-4

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1049235101

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/11132188


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0299", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Other Physical Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/02", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Physical Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Membrane", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Nuclear Magnetic Resonance, Biomolecular", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Phospholipids", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Phosphorus Isotopes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protons", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sea Urchins", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Amherst College", 
          "id": "https://www.grid.ac/institutes/grid.252152.3", 
          "name": [
            "Department of Biology, Amherst College, 01002, Amherst, Massachusetts"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Larijani", 
        "givenName": "B.", 
        "id": "sg:person.0750154531.48", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0750154531.48"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Amherst College", 
          "id": "https://www.grid.ac/institutes/grid.252152.3", 
          "name": [
            "Department of Biology, Amherst College, 01002, Amherst, Massachusetts"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Poccia", 
        "givenName": "D. L.", 
        "id": "sg:person.01060016271.61", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01060016271.61"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Massachusetts Amherst", 
          "id": "https://www.grid.ac/institutes/grid.266683.f", 
          "name": [
            "Conte National Center for Polymer Research, Department of Polymer Science and Engineering, University of Massachusetts at Amherst, 01003, Amherst, Massachusetts"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Dickinson", 
        "givenName": "L. Charles", 
        "id": "sg:person.072644330.63", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.072644330.63"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/0005-2760(95)00181-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002649411"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0022-2364(83)90241-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012757681"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/mrm.1910210113", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014634031"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0022-2364(91)90228-l", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016071272"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0091-679x(08)60889-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018911209"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/mrm.1910290603", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026902695"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0009-2614(80)80041-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040738091"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/mrm.1910260106", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040744880"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/1097-0142(19951115)76:10<1715::aid-cncr2820761007>3.0.co;2-d", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042349454"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0962-8924(97)01196-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049570756"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/mrc.1260290610", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050248861"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/mrc.1260290610", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050248861"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0022-2364(85)90018-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052101927"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078667551", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082912454", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082959282", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082994188", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2000-11", 
    "datePublishedReg": "2000-11-01", 
    "description": "An approach to the direct quantification of phospholipids from two-dimensional 31P-1H nuclear magnetic resonance (NMR) spectroscopy with isotropic proton mixing has been developed as a general method for phospholipid analysis of minor membrane vesicle subfractions. Membrane vesicles were subfractionated by sedimentation to density equilibrium in a sucrose gradient, and a modified Folch method was employed to extract their phospholipids. The coefficient for the NMR detection efficiency of each phospholipid and the relative mole percentage of the phospholipids present in the membrane vesicles were calculated. We demonstrate low detection limits such that relative concentrations of phospholipids in membrane subfractions may be determined even in the submicromolar range.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s11745-000-0645-4", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.2592369", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1006395", 
        "issn": [
          "0024-4201", 
          "1558-9307"
        ], 
        "name": "Lipids", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "11", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "35"
      }
    ], 
    "name": "Phospholipid identification and quantification of membrane vesicle subfractions by 31P\u22121H two-dimensional nuclear magnetic resonance", 
    "pagination": "1289-1297", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "ea8bb1cfb914fd30be7b20a3b21ed4837e869b4d2efb8ae754793e0db9a5cd2f"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "11132188"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0060450"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s11745-000-0645-4"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1049235101"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s11745-000-0645-4", 
      "https://app.dimensions.ai/details/publication/pub.1049235101"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T01:10", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8697_00000524.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007%2Fs11745-000-0645-4"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s11745-000-0645-4'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s11745-000-0645-4'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s11745-000-0645-4'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s11745-000-0645-4'


 

This table displays all metadata directly associated to this object as RDF triples.

160 TRIPLES      21 PREDICATES      52 URIs      28 LITERALS      16 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s11745-000-0645-4 schema:about N02b8793068d347709e5ebaed4ecfdbde
2 N2c6cc888ec8a423fab228be35270477f
3 N3538a53c0ed34b58937273668f0856aa
4 N3d3186cb75164c3c90f1cf0cca5fcc65
5 N45c2c4263527484f822cbe164dfa7306
6 N82539575281545c487b705100d6eeaba
7 Nf5de9ee7bb7940db9b684443adc4c1a0
8 anzsrc-for:02
9 anzsrc-for:0299
10 schema:author N21584601e59649f685762c3df2ac51c4
11 schema:citation https://app.dimensions.ai/details/publication/pub.1078667551
12 https://app.dimensions.ai/details/publication/pub.1082912454
13 https://app.dimensions.ai/details/publication/pub.1082959282
14 https://app.dimensions.ai/details/publication/pub.1082994188
15 https://doi.org/10.1002/1097-0142(19951115)76:10<1715::aid-cncr2820761007>3.0.co;2-d
16 https://doi.org/10.1002/mrc.1260290610
17 https://doi.org/10.1002/mrm.1910210113
18 https://doi.org/10.1002/mrm.1910260106
19 https://doi.org/10.1002/mrm.1910290603
20 https://doi.org/10.1016/0005-2760(95)00181-6
21 https://doi.org/10.1016/0009-2614(80)80041-8
22 https://doi.org/10.1016/0022-2364(83)90241-x
23 https://doi.org/10.1016/0022-2364(85)90018-6
24 https://doi.org/10.1016/0022-2364(91)90228-l
25 https://doi.org/10.1016/s0091-679x(08)60889-4
26 https://doi.org/10.1016/s0962-8924(97)01196-3
27 schema:datePublished 2000-11
28 schema:datePublishedReg 2000-11-01
29 schema:description An approach to the direct quantification of phospholipids from two-dimensional 31P-1H nuclear magnetic resonance (NMR) spectroscopy with isotropic proton mixing has been developed as a general method for phospholipid analysis of minor membrane vesicle subfractions. Membrane vesicles were subfractionated by sedimentation to density equilibrium in a sucrose gradient, and a modified Folch method was employed to extract their phospholipids. The coefficient for the NMR detection efficiency of each phospholipid and the relative mole percentage of the phospholipids present in the membrane vesicles were calculated. We demonstrate low detection limits such that relative concentrations of phospholipids in membrane subfractions may be determined even in the submicromolar range.
30 schema:genre research_article
31 schema:inLanguage en
32 schema:isAccessibleForFree false
33 schema:isPartOf N6bab13df53134c92b1f78e16b2923d4c
34 Nee9f94bb12c441cc8bba38a8ca3cc088
35 sg:journal.1006395
36 schema:name Phospholipid identification and quantification of membrane vesicle subfractions by 31P−1H two-dimensional nuclear magnetic resonance
37 schema:pagination 1289-1297
38 schema:productId N083e59d69ef649c1be1526ce429cc224
39 N269217066e764dd0a9c997a33ca83101
40 N53d059e0f68d464aa39d784d9a4e6b4a
41 N590c28dec77b45a095de36d7bfba7981
42 N8a5d564a15714c0993e2587fbd9d0eb7
43 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049235101
44 https://doi.org/10.1007/s11745-000-0645-4
45 schema:sdDatePublished 2019-04-11T01:10
46 schema:sdLicense https://scigraph.springernature.com/explorer/license/
47 schema:sdPublisher Nf8123774cb284ca1957622775380abf0
48 schema:url http://link.springer.com/10.1007%2Fs11745-000-0645-4
49 sgo:license sg:explorer/license/
50 sgo:sdDataset articles
51 rdf:type schema:ScholarlyArticle
52 N02b8793068d347709e5ebaed4ecfdbde schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
53 schema:name Phospholipids
54 rdf:type schema:DefinedTerm
55 N083e59d69ef649c1be1526ce429cc224 schema:name nlm_unique_id
56 schema:value 0060450
57 rdf:type schema:PropertyValue
58 N21584601e59649f685762c3df2ac51c4 rdf:first sg:person.0750154531.48
59 rdf:rest N63ea44c041ae472598ebc5856d082210
60 N269217066e764dd0a9c997a33ca83101 schema:name pubmed_id
61 schema:value 11132188
62 rdf:type schema:PropertyValue
63 N2c6cc888ec8a423fab228be35270477f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
64 schema:name Cell Membrane
65 rdf:type schema:DefinedTerm
66 N2dc565cf644141efaf88a39f6fbd7aa4 rdf:first sg:person.072644330.63
67 rdf:rest rdf:nil
68 N3538a53c0ed34b58937273668f0856aa schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
69 schema:name Phosphorus Isotopes
70 rdf:type schema:DefinedTerm
71 N3d3186cb75164c3c90f1cf0cca5fcc65 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
72 schema:name Nuclear Magnetic Resonance, Biomolecular
73 rdf:type schema:DefinedTerm
74 N45c2c4263527484f822cbe164dfa7306 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
75 schema:name Animals
76 rdf:type schema:DefinedTerm
77 N53d059e0f68d464aa39d784d9a4e6b4a schema:name dimensions_id
78 schema:value pub.1049235101
79 rdf:type schema:PropertyValue
80 N590c28dec77b45a095de36d7bfba7981 schema:name readcube_id
81 schema:value ea8bb1cfb914fd30be7b20a3b21ed4837e869b4d2efb8ae754793e0db9a5cd2f
82 rdf:type schema:PropertyValue
83 N63ea44c041ae472598ebc5856d082210 rdf:first sg:person.01060016271.61
84 rdf:rest N2dc565cf644141efaf88a39f6fbd7aa4
85 N6bab13df53134c92b1f78e16b2923d4c schema:issueNumber 11
86 rdf:type schema:PublicationIssue
87 N82539575281545c487b705100d6eeaba schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
88 schema:name Sea Urchins
89 rdf:type schema:DefinedTerm
90 N8a5d564a15714c0993e2587fbd9d0eb7 schema:name doi
91 schema:value 10.1007/s11745-000-0645-4
92 rdf:type schema:PropertyValue
93 Nee9f94bb12c441cc8bba38a8ca3cc088 schema:volumeNumber 35
94 rdf:type schema:PublicationVolume
95 Nf5de9ee7bb7940db9b684443adc4c1a0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
96 schema:name Protons
97 rdf:type schema:DefinedTerm
98 Nf8123774cb284ca1957622775380abf0 schema:name Springer Nature - SN SciGraph project
99 rdf:type schema:Organization
100 anzsrc-for:02 schema:inDefinedTermSet anzsrc-for:
101 schema:name Physical Sciences
102 rdf:type schema:DefinedTerm
103 anzsrc-for:0299 schema:inDefinedTermSet anzsrc-for:
104 schema:name Other Physical Sciences
105 rdf:type schema:DefinedTerm
106 sg:grant.2592369 http://pending.schema.org/fundedItem sg:pub.10.1007/s11745-000-0645-4
107 rdf:type schema:MonetaryGrant
108 sg:journal.1006395 schema:issn 0024-4201
109 1558-9307
110 schema:name Lipids
111 rdf:type schema:Periodical
112 sg:person.01060016271.61 schema:affiliation https://www.grid.ac/institutes/grid.252152.3
113 schema:familyName Poccia
114 schema:givenName D. L.
115 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01060016271.61
116 rdf:type schema:Person
117 sg:person.072644330.63 schema:affiliation https://www.grid.ac/institutes/grid.266683.f
118 schema:familyName Dickinson
119 schema:givenName L. Charles
120 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.072644330.63
121 rdf:type schema:Person
122 sg:person.0750154531.48 schema:affiliation https://www.grid.ac/institutes/grid.252152.3
123 schema:familyName Larijani
124 schema:givenName B.
125 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0750154531.48
126 rdf:type schema:Person
127 https://app.dimensions.ai/details/publication/pub.1078667551 schema:CreativeWork
128 https://app.dimensions.ai/details/publication/pub.1082912454 schema:CreativeWork
129 https://app.dimensions.ai/details/publication/pub.1082959282 schema:CreativeWork
130 https://app.dimensions.ai/details/publication/pub.1082994188 schema:CreativeWork
131 https://doi.org/10.1002/1097-0142(19951115)76:10<1715::aid-cncr2820761007>3.0.co;2-d schema:sameAs https://app.dimensions.ai/details/publication/pub.1042349454
132 rdf:type schema:CreativeWork
133 https://doi.org/10.1002/mrc.1260290610 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050248861
134 rdf:type schema:CreativeWork
135 https://doi.org/10.1002/mrm.1910210113 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014634031
136 rdf:type schema:CreativeWork
137 https://doi.org/10.1002/mrm.1910260106 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040744880
138 rdf:type schema:CreativeWork
139 https://doi.org/10.1002/mrm.1910290603 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026902695
140 rdf:type schema:CreativeWork
141 https://doi.org/10.1016/0005-2760(95)00181-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002649411
142 rdf:type schema:CreativeWork
143 https://doi.org/10.1016/0009-2614(80)80041-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040738091
144 rdf:type schema:CreativeWork
145 https://doi.org/10.1016/0022-2364(83)90241-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1012757681
146 rdf:type schema:CreativeWork
147 https://doi.org/10.1016/0022-2364(85)90018-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052101927
148 rdf:type schema:CreativeWork
149 https://doi.org/10.1016/0022-2364(91)90228-l schema:sameAs https://app.dimensions.ai/details/publication/pub.1016071272
150 rdf:type schema:CreativeWork
151 https://doi.org/10.1016/s0091-679x(08)60889-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018911209
152 rdf:type schema:CreativeWork
153 https://doi.org/10.1016/s0962-8924(97)01196-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049570756
154 rdf:type schema:CreativeWork
155 https://www.grid.ac/institutes/grid.252152.3 schema:alternateName Amherst College
156 schema:name Department of Biology, Amherst College, 01002, Amherst, Massachusetts
157 rdf:type schema:Organization
158 https://www.grid.ac/institutes/grid.266683.f schema:alternateName University of Massachusetts Amherst
159 schema:name Conte National Center for Polymer Research, Department of Polymer Science and Engineering, University of Massachusetts at Amherst, 01003, Amherst, Massachusetts
160 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...