Transcriptional profiling by RNA sequencing of black pepper (Piper nigrum L.) roots infected by Fusarium solani f. sp. piperis View Full Text


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Article Info

DATE

2017-09-21

AUTHORS

Edith C. O. Moreira, Daniel G. Pinheiro, Sheila M. C. Gordo, Simone M. Rodrigues, Elaine Pessoa, Hubert Schaller, Oriel F. de Lemos, Artur Silva, Horacio Schneider, Wilson A. Silva, Iracilda Sampaio, Sylvain Darnet

ABSTRACT

Black pepper (Piper nigrum L.) is the most traded spice worldwide and therefore has great economic value. One of the major limitations of its production is yield losses in fields due to root rot, a disease caused by Fusarium solani f. sp. piperis. This soil-borne pathogen disseminates rapidly in tropical countries. Biotechnological breeding is often presented as an efficient tool to produce resistant pepper cultivars. An RNA-sequencing experiment was used to take a snapshot of the root transcriptome 60 days after infection by the pathogen. The mapping of 67 million SOLiD single-end reads to the Fusarium genome identified many fungal transcripts coding constitutive proteins and two proteins involved in virulence and conidial formation. The annotation of black pepper transcripts obtained by de novo assembly predicted three proteins restricted to this species. While these transcripts were upregulated in infected roots, the corresponding predicted proteins had no hit in databases. A global analysis of differentially expressed black pepper genes, using terms of gene ontology, has demonstrated an enrichment of genes involved in proteolysis, plastid degradation, cell-wall remodeling and secondary metabolism, consistent with toxicity and necrotrophic fungal interactions that force plants to collaborate at the metabolic level. Detailed descriptions of up- or downregulated genes associated with plant defense suggested several genes implied in the biosynthesis of isoprenoids, especially for an upregulated terpene synthase, a putative geranylgeranyl diphosphate synthase, and a downregulated brassinosteroid signaling component (BAK1). These results provide new clues for investigating further target genes and biotechnological approaches for breeding black pepper resistance to Fusarium. More... »

PAGES

239

References to SciGraph publications

  • 2016-06-16. Heat shock protein 90 is required for sexual and asexual development, virulence, and heat shock response in Fusarium graminearum in SCIENTIFIC REPORTS
  • 2016-11-02. Tetrapod limb and sarcopterygian fin regeneration share a core genetic programme in NATURE COMMUNICATIONS
  • 2016-06-30. Susceptibility of in vitro black pepper plant to the filtrate from a Fusarium solani f. sp. piperis culture in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 2005-07. Root Defense Responses to Fungal Pathogens: A Molecular Perspective in PLANT AND SOIL
  • 2014-07-07. Root defense analysis against Fusarium oxysporum reveals new regulators to confer resistance in SCIENTIFIC REPORTS
  • 2008-04-26. Identification of up-regulated genes during zearalenone biosynthesis in Fusarium in EUROPEAN JOURNAL OF PLANT PATHOLOGY
  • 2012-12-20. In silico Characterisation and Phylogenetic Analysis of Two Evolutionarily Conserved miRNAs (miR166 and miR171) from Black Pepper (Piper nigrum L.) in PLANT MOLECULAR BIOLOGY REPORTER
  • 2009-07-08. Isolation and characterization of microsatellite loci in the black pepper, Piper nigrum L. (piperaceae) in CONSERVATION GENETICS RESOURCES
  • 2011-05-25. Identification of sequences expressed during compatible black pepper—Fusarium solani f. sp. piperis interaction in ACTA PHYSIOLOGIAE PLANTARUM
  • 2012-12. Application of MapMan and RiceNet drives systematic analyses of the early heat stress transcriptome in rice seedlings in JOURNAL OF PLANT BIOLOGY
  • <error retrieving object. in <ERROR RETRIEVING OBJECT
  • 2007-06-29. A preliminary assessment of genetic relationships among agronomically important cultivars of black pepper in BMC GENOMIC DATA
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    20 schema:description Black pepper (Piper nigrum L.) is the most traded spice worldwide and therefore has great economic value. One of the major limitations of its production is yield losses in fields due to root rot, a disease caused by Fusarium solani f. sp. piperis. This soil-borne pathogen disseminates rapidly in tropical countries. Biotechnological breeding is often presented as an efficient tool to produce resistant pepper cultivars. An RNA-sequencing experiment was used to take a snapshot of the root transcriptome 60 days after infection by the pathogen. The mapping of 67 million SOLiD single-end reads to the Fusarium genome identified many fungal transcripts coding constitutive proteins and two proteins involved in virulence and conidial formation. The annotation of black pepper transcripts obtained by de novo assembly predicted three proteins restricted to this species. While these transcripts were upregulated in infected roots, the corresponding predicted proteins had no hit in databases. A global analysis of differentially expressed black pepper genes, using terms of gene ontology, has demonstrated an enrichment of genes involved in proteolysis, plastid degradation, cell-wall remodeling and secondary metabolism, consistent with toxicity and necrotrophic fungal interactions that force plants to collaborate at the metabolic level. Detailed descriptions of up- or downregulated genes associated with plant defense suggested several genes implied in the biosynthesis of isoprenoids, especially for an upregulated terpene synthase, a putative geranylgeranyl diphosphate synthase, and a downregulated brassinosteroid signaling component (BAK1). These results provide new clues for investigating further target genes and biotechnological approaches for breeding black pepper resistance to Fusarium.
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