Overexpressions of dihydroxyacetone synthase and dihydroxyacetone kinase in chloroplasts install a novel photosynthetic HCHO-assimilation pathway in transgenic tobacco using modified ... View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2012-04-22

AUTHORS

Su-Qin Xiao, Zhen Sun, Sha-Sha Wang, Jing Zhang, Kun-Zhi Li, Li-Mei Chen

ABSTRACT

Dihydroxyacetone synthase (DAS) and dihydroxyacetone kinase (DAK) are two key enzymes for formaldehyde assimilation in methylotrophic yeasts. In order to using a Gateway LR recombination reaction to construct a plant expression vector that contains the expression cassettes for the das and dak genes and allow the proteins encoded by the two target genes to be localized to the chloroplasts of transgenic plants, the entry vector pEN-L4*-PrbcS-*T-gfp-L3* contained the tomato rbcS 3C promoter (PrbcS) with its transit peptide sequence (*T) and a GFP reporter gene (gfp) was constructed in this study. To verify the applicability of pEN-L4*-PrbcS-*T-gfp-L3*, we generated an entry vector for the dak gene by replacing the gfp gene in this entry vector with the dak gene. We also generated an entry vector for the das gene by replacing the gus gene in another entry vector (pENTR*-PrbcS-*T-gus) with the das gene. Using these entry vectors and pK7m34GW2-8m21GW3, we successfully constructed the pKm-35S-PrbcS-*T-gfp-PROLD-PrbcS-*T-gus and the pKm-35S-PrbcS-*T-dak-PROLD-PrbcS-*T-das expression vectors. Our results showed that high expression of GUS was achieved in leaves, and the expressed GFP, DAS and DAK proteins could be targeted to the chloroplasts after the two expression vectors were used to transform tobacco. The overexpressions of DAS and DAK in the chloroplasts successfully created a novel photosynthetic HCHO-assimilation pathway in transgenic tobacco. By utilizing these expression vectors, we not only successfully expressed two target genes with one transformation but also localized the expressed proteins to chloroplasts via the transit peptide sequence (*T). Therefore, the construction of pEN-L4*-PrbcS-*T-gfp-L3* establishes a technique platform that provides a convenient means for chloroplast genetic engineering. More... »

PAGES

1975-1985

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s11738-012-0998-7

DOI

http://dx.doi.org/10.1007/s11738-012-0998-7

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1046894330


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0607", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Plant Biology", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Faculty of Life Science and Biotechnology, Chenggong Campus, Kunming University of Science and Technology, 650500, Chenggong, Kunming, China", 
          "id": "http://www.grid.ac/institutes/grid.218292.2", 
          "name": [
            "Faculty of Environmental Science and Engineering, Chenggong Campus, Kunming University of Science and Technology, 650500, Chenggong, Kunming, China", 
            "Faculty of Life Science and Biotechnology, Chenggong Campus, Kunming University of Science and Technology, 650500, Chenggong, Kunming, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Xiao", 
        "givenName": "Su-Qin", 
        "id": "sg:person.012666522577.49", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012666522577.49"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Faculty of Life Science and Biotechnology, Chenggong Campus, Kunming University of Science and Technology, 650500, Chenggong, Kunming, China", 
          "id": "http://www.grid.ac/institutes/grid.218292.2", 
          "name": [
            "Faculty of Life Science and Biotechnology, Chenggong Campus, Kunming University of Science and Technology, 650500, Chenggong, Kunming, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sun", 
        "givenName": "Zhen", 
        "id": "sg:person.015233344432.42", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015233344432.42"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Faculty of Life Science and Biotechnology, Chenggong Campus, Kunming University of Science and Technology, 650500, Chenggong, Kunming, China", 
          "id": "http://www.grid.ac/institutes/grid.218292.2", 
          "name": [
            "Faculty of Life Science and Biotechnology, Chenggong Campus, Kunming University of Science and Technology, 650500, Chenggong, Kunming, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wang", 
        "givenName": "Sha-Sha", 
        "id": "sg:person.013640403432.10", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013640403432.10"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Faculty of Life Science and Biotechnology, Chenggong Campus, Kunming University of Science and Technology, 650500, Chenggong, Kunming, China", 
          "id": "http://www.grid.ac/institutes/grid.218292.2", 
          "name": [
            "Faculty of Life Science and Biotechnology, Chenggong Campus, Kunming University of Science and Technology, 650500, Chenggong, Kunming, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zhang", 
        "givenName": "Jing", 
        "id": "sg:person.016030725032.78", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016030725032.78"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Faculty of Life Science and Biotechnology, Chenggong Campus, Kunming University of Science and Technology, 650500, Chenggong, Kunming, China", 
          "id": "http://www.grid.ac/institutes/grid.218292.2", 
          "name": [
            "Faculty of Life Science and Biotechnology, Chenggong Campus, Kunming University of Science and Technology, 650500, Chenggong, Kunming, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Li", 
        "givenName": "Kun-Zhi", 
        "id": "sg:person.01034246734.71", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01034246734.71"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Faculty of Life Science and Biotechnology, Chenggong Campus, Kunming University of Science and Technology, 650500, Chenggong, Kunming, China", 
          "id": "http://www.grid.ac/institutes/grid.218292.2", 
          "name": [
            "Faculty of Life Science and Biotechnology, Chenggong Campus, Kunming University of Science and Technology, 650500, Chenggong, Kunming, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Chen", 
        "givenName": "Li-Mei", 
        "id": "sg:person.01102362134.21", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01102362134.21"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/s11103-005-0340-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003043504", 
          "https://doi.org/10.1007/s11103-005-0340-5"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1023/a:1016052416053", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024626877", 
          "https://doi.org/10.1023/a:1016052416053"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt1001-965", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033250335", 
          "https://doi.org/10.1038/nbt1001-965"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10529-010-0324-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050773127", 
          "https://doi.org/10.1007/s10529-010-0324-7"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2012-04-22", 
    "datePublishedReg": "2012-04-22", 
    "description": "Dihydroxyacetone synthase (DAS) and dihydroxyacetone kinase (DAK) are two key enzymes for formaldehyde assimilation in methylotrophic yeasts. In order to using a Gateway LR recombination reaction to construct a plant expression vector that contains the expression cassettes for the das and dak genes and allow the proteins encoded by the two target genes to be localized to the chloroplasts of transgenic plants, the entry vector pEN-L4*-PrbcS-*T-gfp-L3* contained the tomato rbcS 3C promoter (PrbcS) with its transit peptide sequence (*T) and a GFP reporter gene (gfp) was constructed in this study. To verify the applicability of pEN-L4*-PrbcS-*T-gfp-L3*, we generated an entry vector for the dak gene by replacing the gfp gene in this entry vector with the dak gene. We also generated an entry vector for the das gene by replacing the gus gene in another entry vector (pENTR*-PrbcS-*T-gus) with the das gene. Using these entry vectors and pK7m34GW2-8m21GW3, we successfully constructed the pKm-35S-PrbcS-*T-gfp-PROLD-PrbcS-*T-gus and the pKm-35S-PrbcS-*T-dak-PROLD-PrbcS-*T-das expression vectors. Our results showed that high expression of GUS was achieved in leaves, and the expressed GFP, DAS and DAK proteins could be targeted to the chloroplasts after the two expression vectors were used to transform tobacco. The overexpressions of DAS and DAK in the chloroplasts successfully created a novel photosynthetic HCHO-assimilation pathway in transgenic tobacco. By utilizing these expression vectors, we not only successfully expressed two target genes with one transformation but also localized the expressed proteins to chloroplasts via the transit peptide sequence (*T). Therefore, the construction of pEN-L4*-PrbcS-*T-gfp-L3* establishes a technique platform that provides a convenient means for chloroplast genetic engineering.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/s11738-012-0998-7", 
    "isAccessibleForFree": false, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.4913544", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1031087", 
        "issn": [
          "0137-5881", 
          "1861-1664"
        ], 
        "name": "Acta Physiologiae Plantarum", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "5", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "34"
      }
    ], 
    "keywords": [
      "transit peptide sequence", 
      "dihydroxyacetone synthase", 
      "dihydroxyacetone kinase", 
      "entry vector", 
      "expression vector", 
      "transgenic tobacco", 
      "target genes", 
      "DA genes", 
      "chloroplast genetic engineering", 
      "plant expression vector", 
      "Gateway entry vectors", 
      "GFP reporter gene", 
      "peptide sequences", 
      "LR recombination reaction", 
      "transgenic plants", 
      "gus gene", 
      "formaldehyde assimilation", 
      "GFP gene", 
      "reporter gene", 
      "chloroplasts", 
      "methylotrophic yeast", 
      "genetic engineering", 
      "key enzyme", 
      "expression cassette", 
      "genes", 
      "protein", 
      "kinase", 
      "high expression", 
      "overexpression", 
      "synthase", 
      "pathway", 
      "sequence", 
      "tobacco", 
      "technique platform", 
      "GUS", 
      "yeast", 
      "GFP", 
      "promoter", 
      "leaves", 
      "plants", 
      "cassette", 
      "vector", 
      "enzyme", 
      "recombination reaction", 
      "expression", 
      "assimilation", 
      "DA", 
      "GU", 
      "engineering", 
      "convenient means", 
      "study", 
      "transformation", 
      "results", 
      "platform", 
      "reaction", 
      "order", 
      "means", 
      "construction", 
      "applicability", 
      "PRBC"
    ], 
    "name": "Overexpressions of dihydroxyacetone synthase and dihydroxyacetone kinase in chloroplasts install a novel photosynthetic HCHO-assimilation pathway in transgenic tobacco using modified Gateway entry vectors", 
    "pagination": "1975-1985", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1046894330"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s11738-012-0998-7"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s11738-012-0998-7", 
      "https://app.dimensions.ai/details/publication/pub.1046894330"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-11-24T20:55", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20221124/entities/gbq_results/article/article_560.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/s11738-012-0998-7"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s11738-012-0998-7'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s11738-012-0998-7'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s11738-012-0998-7'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s11738-012-0998-7'


 

This table displays all metadata directly associated to this object as RDF triples.

175 TRIPLES      21 PREDICATES      89 URIs      76 LITERALS      6 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s11738-012-0998-7 schema:about anzsrc-for:06
2 anzsrc-for:0604
3 anzsrc-for:0607
4 schema:author N86b29a2a2ae14b36a009cdb5084fdd59
5 schema:citation sg:pub.10.1007/s10529-010-0324-7
6 sg:pub.10.1007/s11103-005-0340-5
7 sg:pub.10.1023/a:1016052416053
8 sg:pub.10.1038/nbt1001-965
9 schema:datePublished 2012-04-22
10 schema:datePublishedReg 2012-04-22
11 schema:description Dihydroxyacetone synthase (DAS) and dihydroxyacetone kinase (DAK) are two key enzymes for formaldehyde assimilation in methylotrophic yeasts. In order to using a Gateway LR recombination reaction to construct a plant expression vector that contains the expression cassettes for the das and dak genes and allow the proteins encoded by the two target genes to be localized to the chloroplasts of transgenic plants, the entry vector pEN-L4*-PrbcS-*T-gfp-L3* contained the tomato rbcS 3C promoter (PrbcS) with its transit peptide sequence (*T) and a GFP reporter gene (gfp) was constructed in this study. To verify the applicability of pEN-L4*-PrbcS-*T-gfp-L3*, we generated an entry vector for the dak gene by replacing the gfp gene in this entry vector with the dak gene. We also generated an entry vector for the das gene by replacing the gus gene in another entry vector (pENTR*-PrbcS-*T-gus) with the das gene. Using these entry vectors and pK7m34GW2-8m21GW3, we successfully constructed the pKm-35S-PrbcS-*T-gfp-PROLD-PrbcS-*T-gus and the pKm-35S-PrbcS-*T-dak-PROLD-PrbcS-*T-das expression vectors. Our results showed that high expression of GUS was achieved in leaves, and the expressed GFP, DAS and DAK proteins could be targeted to the chloroplasts after the two expression vectors were used to transform tobacco. The overexpressions of DAS and DAK in the chloroplasts successfully created a novel photosynthetic HCHO-assimilation pathway in transgenic tobacco. By utilizing these expression vectors, we not only successfully expressed two target genes with one transformation but also localized the expressed proteins to chloroplasts via the transit peptide sequence (*T). Therefore, the construction of pEN-L4*-PrbcS-*T-gfp-L3* establishes a technique platform that provides a convenient means for chloroplast genetic engineering.
12 schema:genre article
13 schema:isAccessibleForFree false
14 schema:isPartOf N7f5d7b71f5f24056a18bbd0399d33f13
15 Nb56bdc2077824f9384ff8ea0533b0ef0
16 sg:journal.1031087
17 schema:keywords DA
18 DA genes
19 GFP
20 GFP gene
21 GFP reporter gene
22 GU
23 GUS
24 Gateway entry vectors
25 LR recombination reaction
26 PRBC
27 applicability
28 assimilation
29 cassette
30 chloroplast genetic engineering
31 chloroplasts
32 construction
33 convenient means
34 dihydroxyacetone kinase
35 dihydroxyacetone synthase
36 engineering
37 entry vector
38 enzyme
39 expression
40 expression cassette
41 expression vector
42 formaldehyde assimilation
43 genes
44 genetic engineering
45 gus gene
46 high expression
47 key enzyme
48 kinase
49 leaves
50 means
51 methylotrophic yeast
52 order
53 overexpression
54 pathway
55 peptide sequences
56 plant expression vector
57 plants
58 platform
59 promoter
60 protein
61 reaction
62 recombination reaction
63 reporter gene
64 results
65 sequence
66 study
67 synthase
68 target genes
69 technique platform
70 tobacco
71 transformation
72 transgenic plants
73 transgenic tobacco
74 transit peptide sequence
75 vector
76 yeast
77 schema:name Overexpressions of dihydroxyacetone synthase and dihydroxyacetone kinase in chloroplasts install a novel photosynthetic HCHO-assimilation pathway in transgenic tobacco using modified Gateway entry vectors
78 schema:pagination 1975-1985
79 schema:productId N1faeeca7ff66478082f035c667bd2fd3
80 Nd21a7a6ed9c044f2bc2b16532ea9c203
81 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046894330
82 https://doi.org/10.1007/s11738-012-0998-7
83 schema:sdDatePublished 2022-11-24T20:55
84 schema:sdLicense https://scigraph.springernature.com/explorer/license/
85 schema:sdPublisher Neac83803de2e4f4c971a1d6e927c187c
86 schema:url https://doi.org/10.1007/s11738-012-0998-7
87 sgo:license sg:explorer/license/
88 sgo:sdDataset articles
89 rdf:type schema:ScholarlyArticle
90 N0ebe50f30752479aa60315d7ea5d60cf rdf:first sg:person.015233344432.42
91 rdf:rest Nf85983f76ef5497b903e70f94646eb15
92 N1faeeca7ff66478082f035c667bd2fd3 schema:name dimensions_id
93 schema:value pub.1046894330
94 rdf:type schema:PropertyValue
95 N23e75342faeb4de5835e3aa7a778f91d rdf:first sg:person.01034246734.71
96 rdf:rest N7233a823f37c4ba692c03366733a90f3
97 N7233a823f37c4ba692c03366733a90f3 rdf:first sg:person.01102362134.21
98 rdf:rest rdf:nil
99 N7f5d7b71f5f24056a18bbd0399d33f13 schema:issueNumber 5
100 rdf:type schema:PublicationIssue
101 N86b29a2a2ae14b36a009cdb5084fdd59 rdf:first sg:person.012666522577.49
102 rdf:rest N0ebe50f30752479aa60315d7ea5d60cf
103 N9c4e2ec453a84932b299d8445f528b68 rdf:first sg:person.016030725032.78
104 rdf:rest N23e75342faeb4de5835e3aa7a778f91d
105 Nb56bdc2077824f9384ff8ea0533b0ef0 schema:volumeNumber 34
106 rdf:type schema:PublicationVolume
107 Nd21a7a6ed9c044f2bc2b16532ea9c203 schema:name doi
108 schema:value 10.1007/s11738-012-0998-7
109 rdf:type schema:PropertyValue
110 Neac83803de2e4f4c971a1d6e927c187c schema:name Springer Nature - SN SciGraph project
111 rdf:type schema:Organization
112 Nf85983f76ef5497b903e70f94646eb15 rdf:first sg:person.013640403432.10
113 rdf:rest N9c4e2ec453a84932b299d8445f528b68
114 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
115 schema:name Biological Sciences
116 rdf:type schema:DefinedTerm
117 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
118 schema:name Genetics
119 rdf:type schema:DefinedTerm
120 anzsrc-for:0607 schema:inDefinedTermSet anzsrc-for:
121 schema:name Plant Biology
122 rdf:type schema:DefinedTerm
123 sg:grant.4913544 http://pending.schema.org/fundedItem sg:pub.10.1007/s11738-012-0998-7
124 rdf:type schema:MonetaryGrant
125 sg:journal.1031087 schema:issn 0137-5881
126 1861-1664
127 schema:name Acta Physiologiae Plantarum
128 schema:publisher Springer Nature
129 rdf:type schema:Periodical
130 sg:person.01034246734.71 schema:affiliation grid-institutes:grid.218292.2
131 schema:familyName Li
132 schema:givenName Kun-Zhi
133 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01034246734.71
134 rdf:type schema:Person
135 sg:person.01102362134.21 schema:affiliation grid-institutes:grid.218292.2
136 schema:familyName Chen
137 schema:givenName Li-Mei
138 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01102362134.21
139 rdf:type schema:Person
140 sg:person.012666522577.49 schema:affiliation grid-institutes:grid.218292.2
141 schema:familyName Xiao
142 schema:givenName Su-Qin
143 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012666522577.49
144 rdf:type schema:Person
145 sg:person.013640403432.10 schema:affiliation grid-institutes:grid.218292.2
146 schema:familyName Wang
147 schema:givenName Sha-Sha
148 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013640403432.10
149 rdf:type schema:Person
150 sg:person.015233344432.42 schema:affiliation grid-institutes:grid.218292.2
151 schema:familyName Sun
152 schema:givenName Zhen
153 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015233344432.42
154 rdf:type schema:Person
155 sg:person.016030725032.78 schema:affiliation grid-institutes:grid.218292.2
156 schema:familyName Zhang
157 schema:givenName Jing
158 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016030725032.78
159 rdf:type schema:Person
160 sg:pub.10.1007/s10529-010-0324-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050773127
161 https://doi.org/10.1007/s10529-010-0324-7
162 rdf:type schema:CreativeWork
163 sg:pub.10.1007/s11103-005-0340-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003043504
164 https://doi.org/10.1007/s11103-005-0340-5
165 rdf:type schema:CreativeWork
166 sg:pub.10.1023/a:1016052416053 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024626877
167 https://doi.org/10.1023/a:1016052416053
168 rdf:type schema:CreativeWork
169 sg:pub.10.1038/nbt1001-965 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033250335
170 https://doi.org/10.1038/nbt1001-965
171 rdf:type schema:CreativeWork
172 grid-institutes:grid.218292.2 schema:alternateName Faculty of Life Science and Biotechnology, Chenggong Campus, Kunming University of Science and Technology, 650500, Chenggong, Kunming, China
173 schema:name Faculty of Environmental Science and Engineering, Chenggong Campus, Kunming University of Science and Technology, 650500, Chenggong, Kunming, China
174 Faculty of Life Science and Biotechnology, Chenggong Campus, Kunming University of Science and Technology, 650500, Chenggong, Kunming, China
175 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...