Effect of plant growth regulators on direct somatic embryogenesis in camphor tree (Cinnamomum camphora L.) from immature zygotic embryos and ... View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2007-12

AUTHORS

Li Du, Suo Zhou, Man-zhu Bao

ABSTRACT

A description of a successful direct somatic embryogenesis induction from immature zygotic embryos of a camphor tree (Cinnamomum camphora L.) is presented. After a subculture of 2–3 years, embryogenic calli could be derived from primary somatic embryos. Immature zygotic embryos were cultured on a Murashige and Skoog (MS) basal medium supplemented with a range of combinations of cytokinins (BA) and auxins (2,4-D or NAA) for somatic embryo induction. Primary somatic embryos could be induced directly in almost all PGR combinations. A positive effect of 2,4-D on somatic embryogenesis from immature zygotic embryos of camphor tree was obtained. BA at appropriate concentrations (< 5 mg·L−1) had an effect similar to 2,4-D, whereas high concentrations (> 5 mg·L−1) of BA had the effect of restraining somatic embryo induction. NAA had a less positive effect on somatic embryogenesis than 2,4-D. More... »

PAGES

267-271

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s11632-007-0042-y

DOI

http://dx.doi.org/10.1007/s11632-007-0042-y

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1049160436


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