Cell suspension culture of Rhizoma zedoariae in a two-stage perfusion bioreactor system for β-elemene production View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2019-04

AUTHORS

Gui Rong Wang, Hui Wang

ABSTRACT

We presented a two-stage combined bioreactor system consisting of a stir-tank and an airlift column, and challenged with Rhizoma zedoariae cell suspensions for β-elemene production. Two-stage culture was initiated when the cell concentration in both vessels was maintained at an appropriate density. The cells were proliferated in stirred-tank with the maximal growth rate of 0.17 d−1 to present enough cells for β-elemene synthesis. In the airlift column, continuous cell separation from culture medium was achieved by using a cell retention device based on centrifugal and gravity settling when the system was performed in perfusion mode. The results indicated that additives can efficiently promote the accumulation of β-elemene in R. zedoariae cells. In addition, the β-elemene content showed higher levels in cell lines of overexpressing 3-hydroxy-3-methylglutaryl coenzyme-A reductase, Farnesyldi phosphate synthase, and ST02C genes. More... »

PAGES

209-220

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s11627-018-9943-9

DOI

http://dx.doi.org/10.1007/s11627-018-9943-9

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1108037958


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