In vitro regeneration of Echinacea purpurea L.: Direct somatic embryogenesis and indirect shoot organogenesis in petiole culture View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2000-01

AUTHORS

Kristen L. Choffe, Jerrin M. R. Victor, Susan J. Murch, Praveen K. Saxena

ABSTRACT

An in vitro propagation system was developed for Echinacea purpurea L. (purple coneflower), a medicinal plant commonly used in the treatment of colds, flu and related ailments. Echinacea seeds were found to be contaminated with systemic fungi and therefore an optimized minimal concentration of Plant Preservation Mixture (PPM) was incorporated in the seed germination medium to recover sterile seedlings. Regeneration was induced on petiole explants from 2-month-old sterile seedlings cultured on medium supplemented with benzylaminopurine (BAP) or thidiazuron (TDZ) in combination with indoleacetic acid (IAA). Two distinct forms of regeneration were identified in cultured petiole explants with histological and morphological observations, viz. the direct formation of somatic embryos on the epidermis and the de novo development of shoots from callus tissues formed in subepidermal cell layers. the results of this study have established a micropropagation system for E. purpurea that will provide sterile plant material for further investigations into medicinally active biochemicals and may facilitate mass production of high-quality E. purpurea plants for the commercial market. More... »

PAGES

30-36

References to SciGraph publications

  • 1993-05. Thidiazuron: a potent cytokinin for woody plant tissue culture in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1996-09. In vitro regeneration of chickpea (Cicer arietinum L.): Stimulation of direct organogenesis and somatic embryogenesis by thidiazuron in PLANT GROWTH REGULATION
  • 1998-10. Thidiazuron: A potent regulator ofin vitro plant morphogenesis in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY - PLANT
  • 1993-04. The use of thidiazuron in tissue culture in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY - PLANT
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s11627-000-0008-4

    DOI

    http://dx.doi.org/10.1007/s11627-000-0008-4

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1043471866


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0607", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Plant Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "University of Guelph", 
              "id": "https://www.grid.ac/institutes/grid.34429.38", 
              "name": [
                "Department of Plant Agriculture, Biotechnology Division, University of Guelph, N1G 2W1, Guelph, Ontario, Canada"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Choffe", 
            "givenName": "Kristen L.", 
            "id": "sg:person.013564505163.31", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013564505163.31"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Guelph", 
              "id": "https://www.grid.ac/institutes/grid.34429.38", 
              "name": [
                "Department of Plant Agriculture, Biotechnology Division, University of Guelph, N1G 2W1, Guelph, Ontario, Canada"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Victor", 
            "givenName": "Jerrin M. R.", 
            "id": "sg:person.013651701257.14", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013651701257.14"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Guelph", 
              "id": "https://www.grid.ac/institutes/grid.34429.38", 
              "name": [
                "Department of Plant Agriculture, Biotechnology Division, University of Guelph, N1G 2W1, Guelph, Ontario, Canada"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Murch", 
            "givenName": "Susan J.", 
            "id": "sg:person.01025173415.23", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01025173415.23"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Guelph", 
              "id": "https://www.grid.ac/institutes/grid.34429.38", 
              "name": [
                "Department of Plant Agriculture, Biotechnology Division, University of Guelph, N1G 2W1, Guelph, Ontario, Canada"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Saxena", 
            "givenName": "Praveen K.", 
            "id": "sg:person.01062523774.29", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01062523774.29"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1016/0192-0561(93)90078-d", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001849259"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0192-0561(93)90078-d", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001849259"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf01983223", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001849516", 
              "https://doi.org/10.1007/bf01983223"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf01983223", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001849516", 
              "https://doi.org/10.1007/bf01983223"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1399-3054.1995.tb05311.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011256762"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02632259", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021909136", 
              "https://doi.org/10.1007/bf02632259"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1104/pp.99.4.1704", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021953277"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1399-3054.1962.tb08052.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023703429"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0014-4827(68)90403-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1027478679"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02822732", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031895499", 
              "https://doi.org/10.1007/bf02822732"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02822732", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031895499", 
              "https://doi.org/10.1007/bf02822732"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0031-6989(88)80848-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035257796"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00037796", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1052908914", 
              "https://doi.org/10.1007/bf00037796"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00037796", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1052908914", 
              "https://doi.org/10.1007/bf00037796"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1055/s-2006-959359", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1057471547"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.143.3601.20", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062481511"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1075775490", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1077515408", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1081717087", 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2000-01", 
        "datePublishedReg": "2000-01-01", 
        "description": "An in vitro propagation system was developed for Echinacea purpurea L. (purple coneflower), a medicinal plant commonly used in the treatment of colds, flu and related ailments. Echinacea seeds were found to be contaminated with systemic fungi and therefore an optimized minimal concentration of Plant Preservation Mixture (PPM) was incorporated in the seed germination medium to recover sterile seedlings. Regeneration was induced on petiole explants from 2-month-old sterile seedlings cultured on medium supplemented with benzylaminopurine (BAP) or thidiazuron (TDZ) in combination with indoleacetic acid (IAA). Two distinct forms of regeneration were identified in cultured petiole explants with histological and morphological observations, viz. the direct formation of somatic embryos on the epidermis and the de novo development of shoots from callus tissues formed in subepidermal cell layers. the results of this study have established a micropropagation system for E. purpurea that will provide sterile plant material for further investigations into medicinally active biochemicals and may facilitate mass production of high-quality E. purpurea plants for the commercial market.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1007/s11627-000-0008-4", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1101676", 
            "issn": [
              "1054-5476", 
              "1475-2689"
            ], 
            "name": "In Vitro Cellular & Developmental Biology - Plant", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "36"
          }
        ], 
        "name": "In vitro regeneration of Echinacea purpurea L.: Direct somatic embryogenesis and indirect shoot organogenesis in petiole culture", 
        "pagination": "30-36", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "cf12961024889edf2e09f9ed5249b0a976319717f790f600148dc3336c43e2bd"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/s11627-000-0008-4"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1043471866"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/s11627-000-0008-4", 
          "https://app.dimensions.ai/details/publication/pub.1043471866"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-10T13:20", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8659_00000523.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://link.springer.com/10.1007%2Fs11627-000-0008-4"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s11627-000-0008-4'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s11627-000-0008-4'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s11627-000-0008-4'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s11627-000-0008-4'


     

    This table displays all metadata directly associated to this object as RDF triples.

    128 TRIPLES      21 PREDICATES      42 URIs      19 LITERALS      7 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/s11627-000-0008-4 schema:about anzsrc-for:06
    2 anzsrc-for:0607
    3 schema:author Nc1261f3f93b34e8e99cdd01da25eb512
    4 schema:citation sg:pub.10.1007/bf00037796
    5 sg:pub.10.1007/bf01983223
    6 sg:pub.10.1007/bf02632259
    7 sg:pub.10.1007/bf02822732
    8 https://app.dimensions.ai/details/publication/pub.1075775490
    9 https://app.dimensions.ai/details/publication/pub.1077515408
    10 https://app.dimensions.ai/details/publication/pub.1081717087
    11 https://doi.org/10.1016/0014-4827(68)90403-5
    12 https://doi.org/10.1016/0192-0561(93)90078-d
    13 https://doi.org/10.1016/s0031-6989(88)80848-8
    14 https://doi.org/10.1055/s-2006-959359
    15 https://doi.org/10.1104/pp.99.4.1704
    16 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
    17 https://doi.org/10.1111/j.1399-3054.1995.tb05311.x
    18 https://doi.org/10.1126/science.143.3601.20
    19 schema:datePublished 2000-01
    20 schema:datePublishedReg 2000-01-01
    21 schema:description An in vitro propagation system was developed for Echinacea purpurea L. (purple coneflower), a medicinal plant commonly used in the treatment of colds, flu and related ailments. Echinacea seeds were found to be contaminated with systemic fungi and therefore an optimized minimal concentration of Plant Preservation Mixture (PPM) was incorporated in the seed germination medium to recover sterile seedlings. Regeneration was induced on petiole explants from 2-month-old sterile seedlings cultured on medium supplemented with benzylaminopurine (BAP) or thidiazuron (TDZ) in combination with indoleacetic acid (IAA). Two distinct forms of regeneration were identified in cultured petiole explants with histological and morphological observations, viz. the direct formation of somatic embryos on the epidermis and the de novo development of shoots from callus tissues formed in subepidermal cell layers. the results of this study have established a micropropagation system for E. purpurea that will provide sterile plant material for further investigations into medicinally active biochemicals and may facilitate mass production of high-quality E. purpurea plants for the commercial market.
    22 schema:genre research_article
    23 schema:inLanguage en
    24 schema:isAccessibleForFree false
    25 schema:isPartOf N1efe9b0d756341ddb70c52b864d701f0
    26 Nbbb4fb05f9934d6b8eb9975df0a6e651
    27 sg:journal.1101676
    28 schema:name In vitro regeneration of Echinacea purpurea L.: Direct somatic embryogenesis and indirect shoot organogenesis in petiole culture
    29 schema:pagination 30-36
    30 schema:productId N28718959f4114d80aa4e140c88165450
    31 N52071416dc33411890af8039bb31020c
    32 Nb3a113a982cc49dc8b9f06ee67cc0fe1
    33 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043471866
    34 https://doi.org/10.1007/s11627-000-0008-4
    35 schema:sdDatePublished 2019-04-10T13:20
    36 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    37 schema:sdPublisher Nfe2bf33bcd5e496d8deaa017f0cd5476
    38 schema:url http://link.springer.com/10.1007%2Fs11627-000-0008-4
    39 sgo:license sg:explorer/license/
    40 sgo:sdDataset articles
    41 rdf:type schema:ScholarlyArticle
    42 N1efe9b0d756341ddb70c52b864d701f0 schema:issueNumber 1
    43 rdf:type schema:PublicationIssue
    44 N28718959f4114d80aa4e140c88165450 schema:name readcube_id
    45 schema:value cf12961024889edf2e09f9ed5249b0a976319717f790f600148dc3336c43e2bd
    46 rdf:type schema:PropertyValue
    47 N52071416dc33411890af8039bb31020c schema:name doi
    48 schema:value 10.1007/s11627-000-0008-4
    49 rdf:type schema:PropertyValue
    50 N565ea5e233b8462290d8582ce3251940 rdf:first sg:person.01025173415.23
    51 rdf:rest Nd8ae0291b535486c82c281e4fb9745ca
    52 Nb3a113a982cc49dc8b9f06ee67cc0fe1 schema:name dimensions_id
    53 schema:value pub.1043471866
    54 rdf:type schema:PropertyValue
    55 Nbbb4fb05f9934d6b8eb9975df0a6e651 schema:volumeNumber 36
    56 rdf:type schema:PublicationVolume
    57 Nc1261f3f93b34e8e99cdd01da25eb512 rdf:first sg:person.013564505163.31
    58 rdf:rest Ne2556bec5f90463ca78d18383b39f7dc
    59 Nd8ae0291b535486c82c281e4fb9745ca rdf:first sg:person.01062523774.29
    60 rdf:rest rdf:nil
    61 Ne2556bec5f90463ca78d18383b39f7dc rdf:first sg:person.013651701257.14
    62 rdf:rest N565ea5e233b8462290d8582ce3251940
    63 Nfe2bf33bcd5e496d8deaa017f0cd5476 schema:name Springer Nature - SN SciGraph project
    64 rdf:type schema:Organization
    65 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    66 schema:name Biological Sciences
    67 rdf:type schema:DefinedTerm
    68 anzsrc-for:0607 schema:inDefinedTermSet anzsrc-for:
    69 schema:name Plant Biology
    70 rdf:type schema:DefinedTerm
    71 sg:journal.1101676 schema:issn 1054-5476
    72 1475-2689
    73 schema:name In Vitro Cellular & Developmental Biology - Plant
    74 rdf:type schema:Periodical
    75 sg:person.01025173415.23 schema:affiliation https://www.grid.ac/institutes/grid.34429.38
    76 schema:familyName Murch
    77 schema:givenName Susan J.
    78 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01025173415.23
    79 rdf:type schema:Person
    80 sg:person.01062523774.29 schema:affiliation https://www.grid.ac/institutes/grid.34429.38
    81 schema:familyName Saxena
    82 schema:givenName Praveen K.
    83 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01062523774.29
    84 rdf:type schema:Person
    85 sg:person.013564505163.31 schema:affiliation https://www.grid.ac/institutes/grid.34429.38
    86 schema:familyName Choffe
    87 schema:givenName Kristen L.
    88 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013564505163.31
    89 rdf:type schema:Person
    90 sg:person.013651701257.14 schema:affiliation https://www.grid.ac/institutes/grid.34429.38
    91 schema:familyName Victor
    92 schema:givenName Jerrin M. R.
    93 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013651701257.14
    94 rdf:type schema:Person
    95 sg:pub.10.1007/bf00037796 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052908914
    96 https://doi.org/10.1007/bf00037796
    97 rdf:type schema:CreativeWork
    98 sg:pub.10.1007/bf01983223 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001849516
    99 https://doi.org/10.1007/bf01983223
    100 rdf:type schema:CreativeWork
    101 sg:pub.10.1007/bf02632259 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021909136
    102 https://doi.org/10.1007/bf02632259
    103 rdf:type schema:CreativeWork
    104 sg:pub.10.1007/bf02822732 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031895499
    105 https://doi.org/10.1007/bf02822732
    106 rdf:type schema:CreativeWork
    107 https://app.dimensions.ai/details/publication/pub.1075775490 schema:CreativeWork
    108 https://app.dimensions.ai/details/publication/pub.1077515408 schema:CreativeWork
    109 https://app.dimensions.ai/details/publication/pub.1081717087 schema:CreativeWork
    110 https://doi.org/10.1016/0014-4827(68)90403-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027478679
    111 rdf:type schema:CreativeWork
    112 https://doi.org/10.1016/0192-0561(93)90078-d schema:sameAs https://app.dimensions.ai/details/publication/pub.1001849259
    113 rdf:type schema:CreativeWork
    114 https://doi.org/10.1016/s0031-6989(88)80848-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035257796
    115 rdf:type schema:CreativeWork
    116 https://doi.org/10.1055/s-2006-959359 schema:sameAs https://app.dimensions.ai/details/publication/pub.1057471547
    117 rdf:type schema:CreativeWork
    118 https://doi.org/10.1104/pp.99.4.1704 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021953277
    119 rdf:type schema:CreativeWork
    120 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1023703429
    121 rdf:type schema:CreativeWork
    122 https://doi.org/10.1111/j.1399-3054.1995.tb05311.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1011256762
    123 rdf:type schema:CreativeWork
    124 https://doi.org/10.1126/science.143.3601.20 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062481511
    125 rdf:type schema:CreativeWork
    126 https://www.grid.ac/institutes/grid.34429.38 schema:alternateName University of Guelph
    127 schema:name Department of Plant Agriculture, Biotechnology Division, University of Guelph, N1G 2W1, Guelph, Ontario, Canada
    128 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...