miR-338-3p regulates osteoclastogenesis via targeting IKKβ gene View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2019-04

AUTHORS

Dequn Niu, Zheng Gong, Xuemin Sun, Jianchang Yuan, Tiantian Zheng, Xun Wang, Xu Fan, Yingji Mao, Xianfu Liu, Baoding Tang, Yingxiao Fu

ABSTRACT

This study determined the effects of miR-338-3p on osteoclast (OC) differentiation and activation. The change levels of miR-338-3p in differentiated OCs were investigated by microRNA microarray assay and quantitative real-time PCR analysis. The effects of miR-338-3p on the differentiation and activation of OCs were determined by tartrate-resistant acid phosphatase staining resorption activity assay and Western blot. Target genes of miR-338-3p were identified by target gene prediction and dual-luciferase reporter gene detection assay as well as Western blot. Results showed that miR-338-3p was markedly downregulated in differentiated OCs. miR-338-3p could inhibit the formation and absorption activity of OCs. Western blot showed that miR-338-3p could influence the change levels of OC differentiation-related proteins. Dual-luciferase reporter gene detection assay and Western blot both showed that miR-338-3p directly targeted IKKβ gene. In conclusion, miR-338-3p may affect the formation and activity of OCs by targeting the IKKβ gene. More... »

PAGES

243-251

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s11626-019-00325-8

DOI

http://dx.doi.org/10.1007/s11626-019-00325-8

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1112855377

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30887211


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