Establishment and characterization of a novel cell line from midgut tissue of Helicoverpa armigera (Lepidoptera: Noctuidae) View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2015-02-13

AUTHORS

Jianghuai Li, Fei He, Yongbo Yang, Yutao Xiao, Rong Peng, Hanchao Yao, Xianchun Li, Jianxin Peng, Huazhu Hong, Kaiyu Liu

ABSTRACT

The midgut of lepidopteran larvae serves as a target for many pathogens such as Bacillus thuringiensis (Bt). Cell lines originating from midgut tissues will be very helpful tools in many research fields. However, to date, no Bt-susceptible midgut-derived cell lines are available. Here, we reported that a novel cell line, designated as HNU-Ha-MG1, was established from midgut tissue of the fourth instar larvae of Helicoverpa armigera. This cell line grew well in Grace’s insect cell culture medium supplemented with 10−15% fetal bovine serum. The shape of the most cells was round or polygonal, and some tended to aggregate to form multiple cell masses. The size of the cells was 13.8 ± 1.8 μm in diameter, and the maximum density reached (2.40 ± 0.15) × 106 cells/ml. The population doubling time during logarithmic growth phase was 58.6 ± 7.0 h at 28°C. The number of chromosomes was about 90–130, which exhibited typical chromosome characteristics of lepidopteran cell lines. The patterns of random amplified polymorphic DNA of the cell line were different from those of Sl-HP and Hi5 cell lines which were frequently used in our laboratory. 20-Hydroxyecdysterone induced apoptosis in a very small part of cells at 2 μg/ml but did not affect expression of autophagy-related protein 8 (Atg8) and its lipidation at 36 h post-treatment. The cell line was permissive to Autographa californica nuclear polyhedrosis virus (AcMNPV) and H. armigera nuclear polyhedrosis virus (HaSNPV). This cell line was found to be susceptible to activated Cry1C at the final concentration of 0.5−1.0 μg/ml but not to the activated Cry1Ac. More... »

PAGES

562-571

References to SciGraph publications

  • 2001. Insect Midgut as a Site for Insecticide Detoxification and Resistance in BIOCHEMICAL SITES OF INSECTICIDE ACTION AND RESISTANCE
  • 2013-10-26. A new insect cell line from the pupal ovary of the Asian corn borer moth Ostrinia furnacalis in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY - ANIMAL
  • 1985-10. A modified technique for the improved characterization of lepidopteran chromosomes from cells in culture in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY - PLANT
  • 2010-10-07. Development and characterization of new cell line BPH22 from midgut epithelial cells of Poekilocerus pictus (Fabricius, 1775) in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY - ANIMAL
  • 2014-11-21. Expression of recombinant and mosaic Cry1Ac receptors from Helicoverpa armigera and their influences on the cytotoxicity of activated Cry1Ac to Spodoptera litura Sl-HP cells in CYTOTECHNOLOGY
  • 2012-05-12. Development of cell lines from the cactophagous insect: Cactoblastis cactorum (Lepidoptera: Pyralidae) and their susceptibility to three baculoviruses in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY - ANIMAL
  • 2014-06-09. ABCC transporters mediate insect resistance to multiple Bt toxins revealed by bulk segregant analysis in BMC BIOLOGY
  • 1983-08. Establishment of a cell line fromHeliothis Armigera (Hbn.) (Lepidoptera: Noctuidae) in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY - PLANT
  • 2008-07. Highly passage of Spodoptera litura cell line causes its permissiveness to baculovirus infection in CYTOTECHNOLOGY
  • 2001-06. Novel techniques to establish new insect cell lines in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY - ANIMAL
  • 2013-01-09. Establishment and characterization of a cell line developed from the neonate larvae of Papilio demoleus Linnaeus (Lepidoptera: Papilionidae) in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY - ANIMAL
  • 1962-08. Establishment of Four Strains of Cells from Insect Tissues Grown in vitro in NATURE
  • 2006-01. Two lepidopteran cell lines stably transformed by the ABC transporter gene PDR5 show tolerance to diacetoxyscirpenol in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY - ANIMAL
  • 2014-01-24. Establishment and characterization of three new cell lines from the embryonic tissue of Holotrichia oblita Faldermann (Coleoptera: Scarabaeidae) in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY - ANIMAL
  • 2002. A NEW CELL LINE FROM THE EMBRYONIC TISSUE OF HELICOVERPA ARMIGERA HBN. (LEPIDOPTERA: NOCTUIDAE) in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY - ANIMAL
  • 2009-02-27. Primary culture of insect midgut cells in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY - ANIMAL
  • 2013-11-07. Establishing midgut cell culture from Rhynchophorus ferrugineus (Olivier) and toxicity assessment against ten different insecticides in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY - ANIMAL
  • 2012-02-01. Serum-free culture of an embryonic cell line from Bombyx mori and reinforcement of susceptibility of a recombinant BmNPV by cooling in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY - ANIMAL
  • 1996. Structure and ultrastructure of the insect midgut in BIOLOGY OF THE INSECT MIDGUT
  • 2012-06-30. A cell line derived from the red flour beetle Tribolium castaneum (Coleoptera: Tenebrionidae) in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY - ANIMAL
  • 2004. SUPPRESSIVE EFFECTS OF ASCORBATE DERIVATIVES ON ULTRAVIOLET-BINDUCED INJURY IN HACAT HUMAN KERATINOCYTES in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY - ANIMAL
  • 2001-06. Primary and continuous midgut cell cultures fromPseudaletia unipuncta (lepidoptera: Noctuidae) in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY - ANIMAL
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s11626-015-9870-6

    DOI

    http://dx.doi.org/10.1007/s11626-015-9870-6

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1028741389

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/25678461


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