Gene expression pattern of some classes of cytochrome P-450 and glutathione S-transferase enzymes in differentiated hepatocytes-like cells from menstrual blood ... View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2015-05

AUTHORS

Aida Esmaeili-Rad, Sayeh Khanjani, Hamidreza Vaziri, Somaieh Kazemnejad

ABSTRACT

Recently, valuable characteristics of menstrual blood stem cells (MenSCs) have impelled scientists to take its advantages for cell therapy of different diseases including liver disorders. In this study, we examined messenger RNA (mRNA) expression levels of phases I and II drug metabolizing enzymes including glutathione S-transferase (GST) and cytochrome P-450 (CYP) in differentiated hepatocyte-like cells from MenSCs. The isolated MenSCs were characterized and differentiated into hepatocyte-like cells using hepatocyte growth factor (HGF) and oncostatin M (OSM) in combination with other components in serum-free culture media. After primary characterization of hepatocyte markers, mRNA expression of GSTA1, GSTA2, GSTP1, CYP3A4, and CYP7A1 was assessed in differentiated cells in reference to undifferentiated cells using real-time PCR. Based on immunofluorescent staining and real-time PCR data, the differentiated MenSCs could express functional hepatocyte markers at mRNA and/or protein levels suggesting development of hepatocyte-like cells from MenSCs. Moreover, the expression levels of GSTA1, GSTA2, and CYP3A4 mRNA were upregulated in differentiated cells compared to undifferentiated cells. The expression of CYP7A1 gene was also remarkable on the last day of differentiation process. However, the expression level of GSTP1 did not exhibit statistically significant change during differentiation (P = 0.6). Based on accumulative data, MenSCs could be viewed as an accessible population of stem cells with differentiation ability into drug-metabolizing hepatocyte-like cells. More... »

PAGES

530-538

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s11626-014-9857-8

DOI

http://dx.doi.org/10.1007/s11626-014-9857-8

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1052275975

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/25614436


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Biomarkers", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Culture Media, Serum-Free", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cytochrome P-450 Enzyme System", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Female", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fluorescent Antibody Technique", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression Regulation, Enzymologic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Glutathione Transferase", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hepatocytes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Menstrual Cycle", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Real-Time Polymerase Chain Reaction", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Stem Cells", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of Guilan", 
          "id": "https://www.grid.ac/institutes/grid.411872.9", 
          "name": [
            "Department of Biology, Faculty of Sciences, University of Guilan, University Campus 2, Rasht, Iran"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Esmaeili-Rad", 
        "givenName": "Aida", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Academic Center for Education, Culture and Research", 
          "id": "https://www.grid.ac/institutes/grid.417689.5", 
          "name": [
            "Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Khanjani", 
        "givenName": "Sayeh", 
        "id": "sg:person.01157671667.76", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01157671667.76"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Guilan", 
          "id": "https://www.grid.ac/institutes/grid.411872.9", 
          "name": [
            "Department of Biology, Faculty of Sciences, University of Guilan, University Campus 2, Rasht, Iran"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Vaziri", 
        "givenName": "Hamidreza", 
        "id": "sg:person.01337032556.44", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01337032556.44"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Academic Center for Education, Culture and Research", 
          "id": "https://www.grid.ac/institutes/grid.417689.5", 
          "name": [
            "Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kazemnejad", 
        "givenName": "Somaieh", 
        "id": "sg:person.0641532123.05", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0641532123.05"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1371/journal.pone.0086075", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000301036"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-3-642-56553-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002304159", 
          "https://doi.org/10.1007/978-3-642-56553-3"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-3-642-56553-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002304159", 
          "https://doi.org/10.1007/978-3-642-56553-3"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/stem.471", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002378715"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0076-6879(05)01002-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002647079"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0010387", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003264234"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1124/jpet.300.2.361", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004821424"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gkp045", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005632264"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1479-5876-5-57", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008086667", 
          "https://doi.org/10.1186/1479-5876-5-57"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0074-7696(06)60002-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010857073"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2007.11.019", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010904856"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s12015-010-9225-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012604717", 
          "https://doi.org/10.1007/s12015-010-9225-3"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s12015-010-9225-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012604717", 
          "https://doi.org/10.1007/s12015-010-9225-3"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.neubiorev.2011.05.010", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013953676"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/7651_2013_9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014139500", 
          "https://doi.org/10.1007/7651_2013_9"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/106002809502900612", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014569266"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/106002809502900612", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014569266"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1447-0756.2011.01800.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015089082"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3727/096368910x532855", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017246688"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3727/096368910x532855", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017246688"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1091/mbc.e06-09-0872", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018598424"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.pharmtox.39.1.1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020832874"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1056/nejmra032424", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024080521"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/term.1715", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026391320"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/toxsci/kfl173", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027475266"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1194/jlr.r900010-jlr200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031349162"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1478-3231.2007.01506.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034113638"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1067/mai.2003.133", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036879313"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.tiv.2007.01.021", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041200236"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/hep.510230432", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041208368"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s12185-012-1067-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041655349", 
          "https://doi.org/10.1007/s12185-012-1067-0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/282615a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041826779", 
          "https://doi.org/10.1038/282615a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1634/stemcells.2007-0826", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045925942"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3727/096368908784153922", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046553639"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3727/096368908784153922", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046553639"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.clinbiochem.2012.01.017", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047863467"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/30.9.e36", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049412343"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1432-1033.1997.00625.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049992118"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jhep.2009.02.029", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050037678"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10517-008-0136-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052948016", 
          "https://doi.org/10.1007/s10517-008-0136-0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10517-008-0136-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052948016", 
          "https://doi.org/10.1007/s10517-008-0136-0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/scd.2005.14.643", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059309501"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/ten.tea.2011.0386", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059315957"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.6248960", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062635849"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci15182", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1063415340"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1387/ijdb.052072hb", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1067190609"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.5301/ijao.5000019", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1072752133"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.5301/ijao.5000019", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1072752133"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074257921", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074960708", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082460802", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082798512", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083278757", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2015-05", 
    "datePublishedReg": "2015-05-01", 
    "description": "Recently, valuable characteristics of menstrual blood stem cells (MenSCs) have impelled scientists to take its advantages for cell therapy of different diseases including liver disorders. In this study, we examined messenger RNA (mRNA) expression levels of phases I and II drug metabolizing enzymes including glutathione S-transferase (GST) and cytochrome P-450 (CYP) in differentiated hepatocyte-like cells from MenSCs. The isolated MenSCs were characterized and differentiated into hepatocyte-like cells using hepatocyte growth factor (HGF) and oncostatin M (OSM) in combination with other components in serum-free culture media. After primary characterization of hepatocyte markers, mRNA expression of GSTA1, GSTA2, GSTP1, CYP3A4, and CYP7A1 was assessed in differentiated cells in reference to undifferentiated cells using real-time PCR. Based on immunofluorescent staining and real-time PCR data, the differentiated MenSCs could express functional hepatocyte markers at mRNA and/or protein levels suggesting development of hepatocyte-like cells from MenSCs. Moreover, the expression levels of GSTA1, GSTA2, and CYP3A4 mRNA were upregulated in differentiated cells compared to undifferentiated cells. The expression of CYP7A1 gene was also remarkable on the last day of differentiation process. However, the expression level of GSTP1 did not exhibit statistically significant change during differentiation (P = 0.6). Based on accumulative data, MenSCs could be viewed as an accessible population of stem cells with differentiation ability into drug-metabolizing hepatocyte-like cells.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s11626-014-9857-8", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1312212", 
        "issn": [
          "1071-2690", 
          "1543-706X"
        ], 
        "name": "In Vitro Cellular & Developmental Biology - Animal", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "5", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "51"
      }
    ], 
    "name": "Gene expression pattern of some classes of cytochrome P-450 and glutathione S-transferase enzymes in differentiated hepatocytes-like cells from menstrual blood stem cells", 
    "pagination": "530-538", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "f9020c441dbf80fa453a00d0736eeea32b043a26849b816feb9e022dbddad97d"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "25614436"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "9418515"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s11626-014-9857-8"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1052275975"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s11626-014-9857-8", 
      "https://app.dimensions.ai/details/publication/pub.1052275975"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T15:06", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8663_00000537.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007%2Fs11626-014-9857-8"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s11626-014-9857-8'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s11626-014-9857-8'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s11626-014-9857-8'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s11626-014-9857-8'


 

This table displays all metadata directly associated to this object as RDF triples.

280 TRIPLES      21 PREDICATES      87 URIs      33 LITERALS      21 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s11626-014-9857-8 schema:about N0e377104e332477fa999ba5dae199a81
2 N170100b94cdf41d59e74945651d844ed
3 N27a517eb0751423c84003bf544e20aef
4 N45b44799b660400b965a9114c63c2044
5 N4c5c65e1186043a3834a11283a1d8d46
6 N6dd75cfec9e045eebaa1b1da560ff892
7 N82b18f6f78ce419e827a7fddd3f73022
8 N9d6d91bc96fe4b469a1b58ca194a368a
9 Nb5c607f16fdb469d8e38140de2bd1dd3
10 Nc98bdddcddbb4e9fa674ac22300a7d78
11 Ne3522750a9ef4077b2dd799fc640217e
12 Nfb67db61207847fb90dc67b4f6025471
13 anzsrc-for:06
14 anzsrc-for:0604
15 schema:author Nc06ea8ce4e1d4bc0ac981708500b6ab6
16 schema:citation sg:pub.10.1007/7651_2013_9
17 sg:pub.10.1007/978-3-642-56553-3
18 sg:pub.10.1007/s10517-008-0136-0
19 sg:pub.10.1007/s12015-010-9225-3
20 sg:pub.10.1007/s12185-012-1067-0
21 sg:pub.10.1038/282615a0
22 sg:pub.10.1186/1479-5876-5-57
23 https://app.dimensions.ai/details/publication/pub.1074257921
24 https://app.dimensions.ai/details/publication/pub.1074960708
25 https://app.dimensions.ai/details/publication/pub.1082460802
26 https://app.dimensions.ai/details/publication/pub.1082798512
27 https://app.dimensions.ai/details/publication/pub.1083278757
28 https://doi.org/10.1002/hep.510230432
29 https://doi.org/10.1002/stem.471
30 https://doi.org/10.1002/term.1715
31 https://doi.org/10.1016/j.cell.2007.11.019
32 https://doi.org/10.1016/j.clinbiochem.2012.01.017
33 https://doi.org/10.1016/j.jhep.2009.02.029
34 https://doi.org/10.1016/j.neubiorev.2011.05.010
35 https://doi.org/10.1016/j.tiv.2007.01.021
36 https://doi.org/10.1016/s0074-7696(06)60002-8
37 https://doi.org/10.1016/s0076-6879(05)01002-5
38 https://doi.org/10.1056/nejmra032424
39 https://doi.org/10.1067/mai.2003.133
40 https://doi.org/10.1089/scd.2005.14.643
41 https://doi.org/10.1089/ten.tea.2011.0386
42 https://doi.org/10.1091/mbc.e06-09-0872
43 https://doi.org/10.1093/nar/30.9.e36
44 https://doi.org/10.1093/nar/gkp045
45 https://doi.org/10.1093/toxsci/kfl173
46 https://doi.org/10.1111/j.1432-1033.1997.00625.x
47 https://doi.org/10.1111/j.1447-0756.2011.01800.x
48 https://doi.org/10.1111/j.1478-3231.2007.01506.x
49 https://doi.org/10.1124/jpet.300.2.361
50 https://doi.org/10.1126/science.6248960
51 https://doi.org/10.1146/annurev.pharmtox.39.1.1
52 https://doi.org/10.1172/jci15182
53 https://doi.org/10.1177/106002809502900612
54 https://doi.org/10.1194/jlr.r900010-jlr200
55 https://doi.org/10.1371/journal.pone.0010387
56 https://doi.org/10.1371/journal.pone.0086075
57 https://doi.org/10.1387/ijdb.052072hb
58 https://doi.org/10.1634/stemcells.2007-0826
59 https://doi.org/10.3727/096368908784153922
60 https://doi.org/10.3727/096368910x532855
61 https://doi.org/10.5301/ijao.5000019
62 schema:datePublished 2015-05
63 schema:datePublishedReg 2015-05-01
64 schema:description Recently, valuable characteristics of menstrual blood stem cells (MenSCs) have impelled scientists to take its advantages for cell therapy of different diseases including liver disorders. In this study, we examined messenger RNA (mRNA) expression levels of phases I and II drug metabolizing enzymes including glutathione S-transferase (GST) and cytochrome P-450 (CYP) in differentiated hepatocyte-like cells from MenSCs. The isolated MenSCs were characterized and differentiated into hepatocyte-like cells using hepatocyte growth factor (HGF) and oncostatin M (OSM) in combination with other components in serum-free culture media. After primary characterization of hepatocyte markers, mRNA expression of GSTA1, GSTA2, GSTP1, CYP3A4, and CYP7A1 was assessed in differentiated cells in reference to undifferentiated cells using real-time PCR. Based on immunofluorescent staining and real-time PCR data, the differentiated MenSCs could express functional hepatocyte markers at mRNA and/or protein levels suggesting development of hepatocyte-like cells from MenSCs. Moreover, the expression levels of GSTA1, GSTA2, and CYP3A4 mRNA were upregulated in differentiated cells compared to undifferentiated cells. The expression of CYP7A1 gene was also remarkable on the last day of differentiation process. However, the expression level of GSTP1 did not exhibit statistically significant change during differentiation (P = 0.6). Based on accumulative data, MenSCs could be viewed as an accessible population of stem cells with differentiation ability into drug-metabolizing hepatocyte-like cells.
65 schema:genre research_article
66 schema:inLanguage en
67 schema:isAccessibleForFree false
68 schema:isPartOf N258ad4107f81420f9acbcaee5056bd4e
69 N966660644590474688f563e4d81e7645
70 sg:journal.1312212
71 schema:name Gene expression pattern of some classes of cytochrome P-450 and glutathione S-transferase enzymes in differentiated hepatocytes-like cells from menstrual blood stem cells
72 schema:pagination 530-538
73 schema:productId N250732b301c1474a8184b92143873961
74 N856f398ced984c1aa87829826005ebec
75 N8a4981d4e4b447fba08f43fbaed85e81
76 N96160c89a4a748719b9a8001d10a9c3d
77 Nde746935902f4b488b494131f9329216
78 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052275975
79 https://doi.org/10.1007/s11626-014-9857-8
80 schema:sdDatePublished 2019-04-10T15:06
81 schema:sdLicense https://scigraph.springernature.com/explorer/license/
82 schema:sdPublisher N3b6cb332758c434e9391114e9218cd94
83 schema:url http://link.springer.com/10.1007%2Fs11626-014-9857-8
84 sgo:license sg:explorer/license/
85 sgo:sdDataset articles
86 rdf:type schema:ScholarlyArticle
87 N0e377104e332477fa999ba5dae199a81 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
88 schema:name Gene Expression Regulation, Enzymologic
89 rdf:type schema:DefinedTerm
90 N170100b94cdf41d59e74945651d844ed schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
91 schema:name Female
92 rdf:type schema:DefinedTerm
93 N250732b301c1474a8184b92143873961 schema:name readcube_id
94 schema:value f9020c441dbf80fa453a00d0736eeea32b043a26849b816feb9e022dbddad97d
95 rdf:type schema:PropertyValue
96 N258ad4107f81420f9acbcaee5056bd4e schema:volumeNumber 51
97 rdf:type schema:PublicationVolume
98 N27a517eb0751423c84003bf544e20aef schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
99 schema:name Culture Media, Serum-Free
100 rdf:type schema:DefinedTerm
101 N3b6cb332758c434e9391114e9218cd94 schema:name Springer Nature - SN SciGraph project
102 rdf:type schema:Organization
103 N45b44799b660400b965a9114c63c2044 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
104 schema:name Fluorescent Antibody Technique
105 rdf:type schema:DefinedTerm
106 N4c5c65e1186043a3834a11283a1d8d46 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
107 schema:name Cytochrome P-450 Enzyme System
108 rdf:type schema:DefinedTerm
109 N6dd75cfec9e045eebaa1b1da560ff892 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
110 schema:name Hepatocytes
111 rdf:type schema:DefinedTerm
112 N784c7d94bf5541d28708b9c41874f230 rdf:first sg:person.01157671667.76
113 rdf:rest Nd4d7ab1e8ad14907a4e55a9a658d80e7
114 N82b18f6f78ce419e827a7fddd3f73022 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
115 schema:name Humans
116 rdf:type schema:DefinedTerm
117 N856f398ced984c1aa87829826005ebec schema:name dimensions_id
118 schema:value pub.1052275975
119 rdf:type schema:PropertyValue
120 N8a4981d4e4b447fba08f43fbaed85e81 schema:name nlm_unique_id
121 schema:value 9418515
122 rdf:type schema:PropertyValue
123 N96160c89a4a748719b9a8001d10a9c3d schema:name pubmed_id
124 schema:value 25614436
125 rdf:type schema:PropertyValue
126 N966660644590474688f563e4d81e7645 schema:issueNumber 5
127 rdf:type schema:PublicationIssue
128 N999c93407ae44797a38af7db8b5cadd8 rdf:first sg:person.0641532123.05
129 rdf:rest rdf:nil
130 N9d6d91bc96fe4b469a1b58ca194a368a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
131 schema:name Glutathione Transferase
132 rdf:type schema:DefinedTerm
133 Na78bb29546a24381ba699a84f70095c7 schema:affiliation https://www.grid.ac/institutes/grid.411872.9
134 schema:familyName Esmaeili-Rad
135 schema:givenName Aida
136 rdf:type schema:Person
137 Nb5c607f16fdb469d8e38140de2bd1dd3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
138 schema:name Real-Time Polymerase Chain Reaction
139 rdf:type schema:DefinedTerm
140 Nc06ea8ce4e1d4bc0ac981708500b6ab6 rdf:first Na78bb29546a24381ba699a84f70095c7
141 rdf:rest N784c7d94bf5541d28708b9c41874f230
142 Nc98bdddcddbb4e9fa674ac22300a7d78 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
143 schema:name Biomarkers
144 rdf:type schema:DefinedTerm
145 Nd4d7ab1e8ad14907a4e55a9a658d80e7 rdf:first sg:person.01337032556.44
146 rdf:rest N999c93407ae44797a38af7db8b5cadd8
147 Nde746935902f4b488b494131f9329216 schema:name doi
148 schema:value 10.1007/s11626-014-9857-8
149 rdf:type schema:PropertyValue
150 Ne3522750a9ef4077b2dd799fc640217e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
151 schema:name Stem Cells
152 rdf:type schema:DefinedTerm
153 Nfb67db61207847fb90dc67b4f6025471 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
154 schema:name Menstrual Cycle
155 rdf:type schema:DefinedTerm
156 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
157 schema:name Biological Sciences
158 rdf:type schema:DefinedTerm
159 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
160 schema:name Genetics
161 rdf:type schema:DefinedTerm
162 sg:journal.1312212 schema:issn 1071-2690
163 1543-706X
164 schema:name In Vitro Cellular & Developmental Biology - Animal
165 rdf:type schema:Periodical
166 sg:person.01157671667.76 schema:affiliation https://www.grid.ac/institutes/grid.417689.5
167 schema:familyName Khanjani
168 schema:givenName Sayeh
169 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01157671667.76
170 rdf:type schema:Person
171 sg:person.01337032556.44 schema:affiliation https://www.grid.ac/institutes/grid.411872.9
172 schema:familyName Vaziri
173 schema:givenName Hamidreza
174 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01337032556.44
175 rdf:type schema:Person
176 sg:person.0641532123.05 schema:affiliation https://www.grid.ac/institutes/grid.417689.5
177 schema:familyName Kazemnejad
178 schema:givenName Somaieh
179 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0641532123.05
180 rdf:type schema:Person
181 sg:pub.10.1007/7651_2013_9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014139500
182 https://doi.org/10.1007/7651_2013_9
183 rdf:type schema:CreativeWork
184 sg:pub.10.1007/978-3-642-56553-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002304159
185 https://doi.org/10.1007/978-3-642-56553-3
186 rdf:type schema:CreativeWork
187 sg:pub.10.1007/s10517-008-0136-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052948016
188 https://doi.org/10.1007/s10517-008-0136-0
189 rdf:type schema:CreativeWork
190 sg:pub.10.1007/s12015-010-9225-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012604717
191 https://doi.org/10.1007/s12015-010-9225-3
192 rdf:type schema:CreativeWork
193 sg:pub.10.1007/s12185-012-1067-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041655349
194 https://doi.org/10.1007/s12185-012-1067-0
195 rdf:type schema:CreativeWork
196 sg:pub.10.1038/282615a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041826779
197 https://doi.org/10.1038/282615a0
198 rdf:type schema:CreativeWork
199 sg:pub.10.1186/1479-5876-5-57 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008086667
200 https://doi.org/10.1186/1479-5876-5-57
201 rdf:type schema:CreativeWork
202 https://app.dimensions.ai/details/publication/pub.1074257921 schema:CreativeWork
203 https://app.dimensions.ai/details/publication/pub.1074960708 schema:CreativeWork
204 https://app.dimensions.ai/details/publication/pub.1082460802 schema:CreativeWork
205 https://app.dimensions.ai/details/publication/pub.1082798512 schema:CreativeWork
206 https://app.dimensions.ai/details/publication/pub.1083278757 schema:CreativeWork
207 https://doi.org/10.1002/hep.510230432 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041208368
208 rdf:type schema:CreativeWork
209 https://doi.org/10.1002/stem.471 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002378715
210 rdf:type schema:CreativeWork
211 https://doi.org/10.1002/term.1715 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026391320
212 rdf:type schema:CreativeWork
213 https://doi.org/10.1016/j.cell.2007.11.019 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010904856
214 rdf:type schema:CreativeWork
215 https://doi.org/10.1016/j.clinbiochem.2012.01.017 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047863467
216 rdf:type schema:CreativeWork
217 https://doi.org/10.1016/j.jhep.2009.02.029 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050037678
218 rdf:type schema:CreativeWork
219 https://doi.org/10.1016/j.neubiorev.2011.05.010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013953676
220 rdf:type schema:CreativeWork
221 https://doi.org/10.1016/j.tiv.2007.01.021 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041200236
222 rdf:type schema:CreativeWork
223 https://doi.org/10.1016/s0074-7696(06)60002-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010857073
224 rdf:type schema:CreativeWork
225 https://doi.org/10.1016/s0076-6879(05)01002-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002647079
226 rdf:type schema:CreativeWork
227 https://doi.org/10.1056/nejmra032424 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024080521
228 rdf:type schema:CreativeWork
229 https://doi.org/10.1067/mai.2003.133 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036879313
230 rdf:type schema:CreativeWork
231 https://doi.org/10.1089/scd.2005.14.643 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059309501
232 rdf:type schema:CreativeWork
233 https://doi.org/10.1089/ten.tea.2011.0386 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059315957
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1091/mbc.e06-09-0872 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018598424
236 rdf:type schema:CreativeWork
237 https://doi.org/10.1093/nar/30.9.e36 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049412343
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1093/nar/gkp045 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005632264
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1093/toxsci/kfl173 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027475266
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1111/j.1432-1033.1997.00625.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1049992118
244 rdf:type schema:CreativeWork
245 https://doi.org/10.1111/j.1447-0756.2011.01800.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1015089082
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1111/j.1478-3231.2007.01506.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1034113638
248 rdf:type schema:CreativeWork
249 https://doi.org/10.1124/jpet.300.2.361 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004821424
250 rdf:type schema:CreativeWork
251 https://doi.org/10.1126/science.6248960 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062635849
252 rdf:type schema:CreativeWork
253 https://doi.org/10.1146/annurev.pharmtox.39.1.1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020832874
254 rdf:type schema:CreativeWork
255 https://doi.org/10.1172/jci15182 schema:sameAs https://app.dimensions.ai/details/publication/pub.1063415340
256 rdf:type schema:CreativeWork
257 https://doi.org/10.1177/106002809502900612 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014569266
258 rdf:type schema:CreativeWork
259 https://doi.org/10.1194/jlr.r900010-jlr200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031349162
260 rdf:type schema:CreativeWork
261 https://doi.org/10.1371/journal.pone.0010387 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003264234
262 rdf:type schema:CreativeWork
263 https://doi.org/10.1371/journal.pone.0086075 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000301036
264 rdf:type schema:CreativeWork
265 https://doi.org/10.1387/ijdb.052072hb schema:sameAs https://app.dimensions.ai/details/publication/pub.1067190609
266 rdf:type schema:CreativeWork
267 https://doi.org/10.1634/stemcells.2007-0826 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045925942
268 rdf:type schema:CreativeWork
269 https://doi.org/10.3727/096368908784153922 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046553639
270 rdf:type schema:CreativeWork
271 https://doi.org/10.3727/096368910x532855 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017246688
272 rdf:type schema:CreativeWork
273 https://doi.org/10.5301/ijao.5000019 schema:sameAs https://app.dimensions.ai/details/publication/pub.1072752133
274 rdf:type schema:CreativeWork
275 https://www.grid.ac/institutes/grid.411872.9 schema:alternateName University of Guilan
276 schema:name Department of Biology, Faculty of Sciences, University of Guilan, University Campus 2, Rasht, Iran
277 rdf:type schema:Organization
278 https://www.grid.ac/institutes/grid.417689.5 schema:alternateName Academic Center for Education, Culture and Research
279 schema:name Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
280 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...