Ontology type: schema:ScholarlyArticle
2008-10
AUTHORSRavi K. Birla, Douglas E. Dow, Yen-Chih Huang, Francesco Migneco, Luda Khait, Gregory H. Borschel, Vikas Dhawan, David L. Brown
ABSTRACTWe have previously described a model to engineer three-dimensional (3-D) heart muscle in vitro. In the current study, we extend our model of 3-D heart muscle to engineer a functional cell-based cardiac pressure generating construct (CPGC). Tubular constructs were fabricated utilizing a phase separation method with chitosan as the scaffolding material. Primary cardiac cells isolated from rat hearts were plated on the surface of fibrin gels cast in 35 mm tissue culture dishes. CPGCs (N = 8) were formed by anchoring the tubular constructs to the center of the plate with primary cardiac cells seeded in fibrin gels wrapped around the tubular constructs. Intraluminal pressure measurements were evaluated with and without external electrical stimulation and histological evaluation performed. The fibrin gel spontaneously compacted due to the traction force of the cardiac cells. By 14 d after original cell plating, the cardiac cells had completely formed a monolayer around the tubular construct resulting in the formation of a cell-based CPGC. The spontaneous contractility of the CPGC was macroscopically visible and resulted in intraluminal pressure spikes of 0.08 mmHg. Upon electrical stimulation, the CPGCs generated twitch pressures of up to 0.05 mmHg. In addition, the CPGC constructs were electrically paced at frequencies of up to 3 Hz. Histological evaluation showed the presence of a continuous cell monolayer around the surface of the tubular construct. In this study, we describe a novel in vitro method to engineer functional cell-based CPGCs and demonstrate several physiological metrics of functional performance. More... »
PAGES340-350
http://scigraph.springernature.com/pub.10.1007/s11626-008-9098-9
DOIhttp://dx.doi.org/10.1007/s11626-008-9098-9
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1026511301
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/18493826
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1102",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Cardiorespiratory Medicine and Haematology",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Medical and Health Sciences",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Animals",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Fibrin",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Heart Ventricles",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Muscle Contraction",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Myocardium",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Myocytes, Cardiac",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Rats",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Rats, Inbred F344",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Tissue Engineering",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "University of Michigan\u2013Ann Arbor",
"id": "https://www.grid.ac/institutes/grid.214458.e",
"name": [
"Section of Cardiac Surgery, University of Michigan, Biomedical Science Research Building, 109 Zina Pitcher Place, Rm. 2018, 48109-2200, Ann Arbor, MI, USA"
],
"type": "Organization"
},
"familyName": "Birla",
"givenName": "Ravi K.",
"id": "sg:person.0776605377.37",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0776605377.37"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of Michigan\u2013Ann Arbor",
"id": "https://www.grid.ac/institutes/grid.214458.e",
"name": [
"Deparment of Biomedical Engineering, The University of Michigan, Ann Arbor, MI, USA"
],
"type": "Organization"
},
"familyName": "Dow",
"givenName": "Douglas E.",
"id": "sg:person.01215440760.11",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01215440760.11"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of Michigan\u2013Ann Arbor",
"id": "https://www.grid.ac/institutes/grid.214458.e",
"name": [
"Deparment of Biomedical Engineering, The University of Michigan, Ann Arbor, MI, USA"
],
"type": "Organization"
},
"familyName": "Huang",
"givenName": "Yen-Chih",
"id": "sg:person.01113033777.51",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01113033777.51"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of Michigan\u2013Ann Arbor",
"id": "https://www.grid.ac/institutes/grid.214458.e",
"name": [
"Section of Cardiac Surgery, University of Michigan, Biomedical Science Research Building, 109 Zina Pitcher Place, Rm. 2018, 48109-2200, Ann Arbor, MI, USA"
],
"type": "Organization"
},
"familyName": "Migneco",
"givenName": "Francesco",
"id": "sg:person.01244247423.51",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01244247423.51"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of Michigan\u2013Ann Arbor",
"id": "https://www.grid.ac/institutes/grid.214458.e",
"name": [
"Section of Cardiac Surgery, University of Michigan, Biomedical Science Research Building, 109 Zina Pitcher Place, Rm. 2018, 48109-2200, Ann Arbor, MI, USA"
],
"type": "Organization"
},
"familyName": "Khait",
"givenName": "Luda",
"id": "sg:person.01224657621.26",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01224657621.26"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of Michigan\u2013Ann Arbor",
"id": "https://www.grid.ac/institutes/grid.214458.e",
"name": [
"Section of Plastic Surgery, The University of Michigan, Ann Arbor, MI, USA"
],
"type": "Organization"
},
"familyName": "Borschel",
"givenName": "Gregory H.",
"id": "sg:person.01372062435.33",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01372062435.33"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of Michigan\u2013Ann Arbor",
"id": "https://www.grid.ac/institutes/grid.214458.e",
"name": [
"Section of Plastic Surgery, The University of Michigan, Ann Arbor, MI, USA"
],
"type": "Organization"
},
"familyName": "Dhawan",
"givenName": "Vikas",
"id": "sg:person.01220263063.61",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01220263063.61"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of Michigan\u2013Ann Arbor",
"id": "https://www.grid.ac/institutes/grid.214458.e",
"name": [
"Section of Plastic Surgery, The University of Michigan, Ann Arbor, MI, USA"
],
"type": "Organization"
},
"familyName": "Brown",
"givenName": "David L.",
"id": "sg:person.0620104677.11",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0620104677.11"
],
"type": "Person"
}
],
"citation": [
{
"id": "sg:pub.10.1007/978-1-4615-8186-4",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1004665387",
"https://doi.org/10.1007/978-1-4615-8186-4"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/978-1-4615-8186-4",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1004665387",
"https://doi.org/10.1007/978-1-4615-8186-4"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/978-1-4615-8186-4",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1004665387",
"https://doi.org/10.1007/978-1-4615-8186-4"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/(sici)1097-0290(19990905)64:5<580::aid-bit8>3.0.co;2-x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1005421380"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0142-9612(96)00167-6",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1007296959"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/jbm.1270",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1007568913"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0022-5223(00)70193-0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1008684845"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0022-5223(00)70193-0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1008684845"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1161/hh0202.103644",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1008977417"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0142-9612(03)00108-x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1009856090"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0142-9612(03)00108-x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1009856090"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1096/fj.04-2034fje",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1010057983"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/(sici)1097-0290(20000405)68:1<106::aid-bit13>3.0.co;2-3",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1015087491"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/(sici)1097-0290(20000405)68:1<106::aid-bit13>3.0.co;2-3",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1015087491"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0142-9612(01)00399-4",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1015146750"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1111/j.1525-1594.2003.07208.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1022948631"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/1097-4636(20010605)55:3<304::aid-jbm1018>3.0.co;2-j",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1029663951"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1161/hh0302.105722",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1030630216"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/(sici)1097-4636(20000315)49:4<534::aid-jbm12>3.0.co;2-#",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1030706241"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/jbm.10176",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1034653075"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/jbm.820271005",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1036758756"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1290/1071-2690(2000)036<0327:eaicpo>2.0.co;2",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1038721677",
"https://doi.org/10.1290/1071-2690(2000)036<0327:eaicpo>2.0.co;2"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0142-9612(99)00011-3",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1039456869"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1023/a:1011600414225",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1039548904",
"https://doi.org/10.1023/a:1011600414225"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0142-9612(03)00026-7",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1044787643"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0142-9612(03)00026-7",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1044787643"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0142-9612(00)00188-5",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1045713053"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0142-9612(98)00159-8",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1046390503"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1089/107632701300062732",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1059205954"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1089/107632703764664800",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1059206230"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1089/ten.1999.5.103",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1059313152"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1161/01.res.81.2.176",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1063341113"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1152/ajpcell.2001.280.2.c288",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1074768217"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.3727/000000001783986684",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1074878981"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.3727/000000001783986684",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1074878981"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1096/fasebj.11.8.9240969",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1083123710"
],
"type": "CreativeWork"
}
],
"datePublished": "2008-10",
"datePublishedReg": "2008-10-01",
"description": "We have previously described a model to engineer three-dimensional (3-D) heart muscle in vitro. In the current study, we extend our model of 3-D heart muscle to engineer a functional cell-based cardiac pressure generating construct (CPGC). Tubular constructs were fabricated utilizing a phase separation method with chitosan as the scaffolding material. Primary cardiac cells isolated from rat hearts were plated on the surface of fibrin gels cast in 35 mm tissue culture dishes. CPGCs (N = 8) were formed by anchoring the tubular constructs to the center of the plate with primary cardiac cells seeded in fibrin gels wrapped around the tubular constructs. Intraluminal pressure measurements were evaluated with and without external electrical stimulation and histological evaluation performed. The fibrin gel spontaneously compacted due to the traction force of the cardiac cells. By 14 d after original cell plating, the cardiac cells had completely formed a monolayer around the tubular construct resulting in the formation of a cell-based CPGC. The spontaneous contractility of the CPGC was macroscopically visible and resulted in intraluminal pressure spikes of 0.08 mmHg. Upon electrical stimulation, the CPGCs generated twitch pressures of up to 0.05 mmHg. In addition, the CPGC constructs were electrically paced at frequencies of up to 3 Hz. Histological evaluation showed the presence of a continuous cell monolayer around the surface of the tubular construct. In this study, we describe a novel in vitro method to engineer functional cell-based CPGCs and demonstrate several physiological metrics of functional performance.",
"genre": "research_article",
"id": "sg:pub.10.1007/s11626-008-9098-9",
"inLanguage": [
"en"
],
"isAccessibleForFree": false,
"isPartOf": [
{
"id": "sg:journal.1312212",
"issn": [
"1071-2690",
"1543-706X"
],
"name": "In Vitro Cellular & Developmental Biology - Animal",
"type": "Periodical"
},
{
"issueNumber": "8-9",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "44"
}
],
"name": "Methodology for the formation of functional, cell-based cardiac pressure generation constructs in vitro",
"pagination": "340-350",
"productId": [
{
"name": "readcube_id",
"type": "PropertyValue",
"value": [
"7e4d08f9a6a8d4d7315fac5eddbd41faf78efefd9bc2039712d83baefdb461e3"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"18493826"
]
},
{
"name": "nlm_unique_id",
"type": "PropertyValue",
"value": [
"9418515"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1007/s11626-008-9098-9"
]
},
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1026511301"
]
}
],
"sameAs": [
"https://doi.org/10.1007/s11626-008-9098-9",
"https://app.dimensions.ai/details/publication/pub.1026511301"
],
"sdDataset": "articles",
"sdDatePublished": "2019-04-10T18:25",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8675_00000536.jsonl",
"type": "ScholarlyArticle",
"url": "http://link.springer.com/10.1007%2Fs11626-008-9098-9"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s11626-008-9098-9'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s11626-008-9098-9'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s11626-008-9098-9'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s11626-008-9098-9'
This table displays all metadata directly associated to this object as RDF triples.
246 TRIPLES
21 PREDICATES
67 URIs
30 LITERALS
18 BLANK NODES