Preperitoneal Fat Grafting Inhibits the Formation of Intra-abdominal Adhesions in Mice View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2019-12-10

AUTHORS

Mervi Laukka, Erika Hoppela, Jemiina Salo, Pia Rantakari, Tove J. Gronroos, Katri Orte, Kaisa Auvinen, Marko Salmi, Heidi Gerke, Kerstin Thol, Emilia Peuhu, Saila Kauhanen, Pirjo Merilahti, Pauliina Hartiala

ABSTRACT

BackgroundAdhesion formation contributes to postoperative complications in abdominal and gynaecological surgery. Thus far, the prevention and treatment strategies have focused on mechanical barriers in solid and liquid form, but these methods are not in routine use. As autologous fat grafting has become popular in treatment of hypertrophic scars because of its immunomodulatory effects, we postulated that fat grafting could also prevent peritoneal adhesion through similar mechanisms.MethodsThis was a control versus intervention study to evaluate the effect of fat grafting in the prevention on peritoneal adhesion formation. An experimental mouse model for moderate and extensive peritoneal adhesions was used (n = 4–6 mice/group). Adhesions were induced mechanically, and a free epididymal fat graft from wild type or CAG-DsRed mice was injected preperitoneally immediately after adhesion induction. PET/CT imaging and scaling of the adhesions were performed, and samples were taken for further analysis at 7 and 30 days postoperation. Macrophage phenotyping was further performed from peritoneal lavage samples, and the expression of inflammatory cytokines and mesothelial layer recovery were analysed from peritoneal tissue samples.ResultsFat grafting significantly inhibited the formation of adhesions. PET/CT results did not show prolonged inflammation in any of the groups. While the expression of anti-inflammatory and anti-fibrotic IL-10 was significantly increased in the peritoneum of the fat graft–treated group at 7 days, tissue-resident and repairing M2 macrophages could no longer be detected in the fat graft at this time point. The percentage of the continuous, healed peritoneum as shown by Keratin 8 staining was greater in the fat graft–treated group after 7 days.ConclusionsFat grafting can inhibit the formation of peritoneal adhesions in mice. Our results suggest that fat grafting promotes the peritoneal healing process in a paracrine manner thereby enabling rapid regeneration of the peritoneal mesothelial cell layer. More... »

PAGES

2838-2848

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s11605-019-04425-4

DOI

http://dx.doi.org/10.1007/s11605-019-04425-4

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1123258854

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/31823326


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39 autologous fat grafting
40 barriers
41 cell layer
42 complications
43 control
44 cytokines
45 days
46 days postoperation
47 effect
48 experimental mouse model
49 expression
50 extensive peritoneal adhesions
51 fat
52 fat grafting
53 fat grafts
54 form
55 formation
56 formation of adhesions
57 graft
58 grafting
59 group
60 gynaecological surgery
61 healing process
62 hypertrophic scars
63 imaging
64 immunomodulatory effects
65 induction
66 inflammation
67 inflammatory cytokines
68 intervention studies
69 intra-abdominal adhesions
70 lavage samples
71 layer
72 liquid form
73 macrophages
74 manner
75 mechanical barrier
76 mechanism
77 mesothelial cell layer
78 method
79 mice
80 model
81 mouse model
82 paracrine manner
83 percentage
84 peritoneal adhesion formation
85 peritoneal adhesions
86 peritoneal lavage samples
87 peritoneal tissue samples
88 peritoneum
89 phenotyping
90 point
91 postoperation
92 postoperative complications
93 preperitoneal fat
94 prevention
95 process
96 prolonged inflammation
97 rapid regeneration
98 recovery
99 regeneration
100 results
101 routine use
102 samples
103 scaling
104 scar
105 similar mechanism
106 staining
107 strategies
108 study
109 surgery
110 time points
111 tissue samples
112 treatment
113 treatment strategies
114 types
115 use
116 wild type
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