MMP-1 Over-expression Promotes Malignancy and Stem-Like Properties of Human Osteosarcoma MG-63 Cells In Vitro View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2018-10

AUTHORS

Man-li Tang, Xiang-jun Bai, Yong Li, Xiao-jing Dai, Fan Yang

ABSTRACT

Osteosarcoma is the most common primary malignant bone tumor in childhood, and it maintains a high level of recurrence. Matrix metalloproteinase-1 (MMP-1) was found to contribute to cancer progression. The present study was to investigate the in vitro effects of MMP-1 over-expression on the proliferation, invasion, metastasis and stem-like properties of osteosarcoma MG-63 cells. The MG-63 cells were cultured and had a full length MMP-1 cDNA inserted by the lentiviral vector (MG-63MMP-1+). MG-63 negative control and MG-63 blank control groups were established as well. MMP-1 expression was detected in MG-63MMP-1+, MG-63 negative control and MG-63 blank control cells using qPCR, Western blotting and immunofluorescence after 24 h of culture. The cell proliferation assay was performed with a camera attached to a bioreactor, which was programmed to photograph five regions of each well every 10 min over a period of 48 h. The cell invasion assay was conducted with Matrigel to assess the invasive potential of MG-63 cells over 24 h, the qPCR analysis to measure stem cell markers, including Oct4, Sox-2, Nanog, and Pax-7, and Western blot analysis to detect invasive and metastatic potential markers TIMP-1, VEGF and BMP2/4, after 24 h of culture. Immunofluorescence was used to investigate the presence of the stem cell marker Pax-7 after 24-h culture. The results showed that over-expression of MMP-1 after transfection could significantly increase tumor cell proliferation and invasion (P<0.05, MG-63MMP-1+versus controls). Pax-7 was highly expressed in MG-63MMP-1+ cells, with no significant changes of Oct-4, Sox-2, and Nanog observed (P<0.05). MG-63MMP-1+ cells showed higher expression of VEGF and BMP 2/4 proteins and lower expression of TIMP-1 protein than controls (P<0.05). It was concluded that MMP-1 over-expression in MG-63 cells contributed to the proliferation, invasion, metastasis and stem-like properties of osteosarcoma cells. Future studies should focus on in vivo effects of MMP-1 over-expression and the application of MMP-1 and Pax-7 inhibition in vivo to osteosarcoma therapies. More... »

PAGES

809-817

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s11596-018-1947-5

DOI

http://dx.doi.org/10.1007/s11596-018-1947-5

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1110953299

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30594980


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1112", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Oncology and Carcinogenesis", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Biomarkers, Tumor", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Bone Neoplasms", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Line, Tumor", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Proliferation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Matrix Metalloproteinase 1", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Neoplasm Invasiveness", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Osteosarcoma", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Tissue Inhibitor of Metalloproteinase-1", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Vascular Endothelial Growth Factor A", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "name": [
            "Department of Traumatic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430030, Wuhan, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Tang", 
        "givenName": "Man-li", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Department of Traumatic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430030, Wuhan, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bai", 
        "givenName": "Xiang-jun", 
        "id": "sg:person.0757422057.71", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0757422057.71"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Steadman Philippon Research Institute", 
          "id": "https://www.grid.ac/institutes/grid.419649.7", 
          "name": [
            "Department of Orthopaedic Surgery, McGovern Medical School, the University of Texas Health Science Center at Houston (UTHealth), 77035, Houston, USA", 
            "Center for Stem Cell and Regenerative Medicine, UTHealth, 77035, Houston, USA", 
            "Center for Regenerative Sports Medicine, Steadman Philippon Research Institute, 81657, Vail, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Li", 
        "givenName": "Yong", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Houston", 
          "id": "https://www.grid.ac/institutes/grid.266436.3", 
          "name": [
            "Department of Pharmacology and Toxicology, College of Pharmacy, the University of Houston, 77555, Houston, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Dai", 
        "givenName": "Xiao-jing", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Steadman Philippon Research Institute", 
          "id": "https://www.grid.ac/institutes/grid.419649.7", 
          "name": [
            "Department of Traumatic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430030, Wuhan, China", 
            "Department of Orthopaedic Surgery, McGovern Medical School, the University of Texas Health Science Center at Houston (UTHealth), 77035, Houston, USA", 
            "Center for Stem Cell and Regenerative Medicine, UTHealth, 77035, Houston, USA", 
            "Center for Regenerative Sports Medicine, Steadman Philippon Research Institute, 81657, Vail, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yang", 
        "givenName": "Fan", 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/978-3-319-04843-7_11", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002069665", 
          "https://doi.org/10.1007/978-3-319-04843-7_11"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.15252/embr.201439246", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002160543"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2353/ajpath.2009.080509", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004391724"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s13277-013-0842-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004513159", 
          "https://doi.org/10.1007/s13277-013-0842-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/cncr.24519", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005687305"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/cncr.24519", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005687305"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/01.blo.0000229333.98781.56", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009210968"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/01.blo.0000229333.98781.56", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009210968"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/mutage/geu045", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011684323"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.matbio.2015.01.019", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012749457"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2010.03.015", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015080267"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.7314/apjcp.2014.15.3.1085", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015776718"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.matbio.2015.01.022", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017053828"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1158/0008-5472.can-08-3580", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020859407"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0098549", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021506355"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ydbio.2004.08.015", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022282991"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3389/fgene.2012.00006", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023813425"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/scbi.2000.0379", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028271714"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbadis.2012.11.006", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029341141"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/jmi.12064", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030779747"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrc3838", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032448549", 
          "https://doi.org/10.1038/nrc3838"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbamcr.2011.04.008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035013562"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.biochi.2004.09.022", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035621430"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2147/ott.s84561", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036265038"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.18632/oncotarget.4902", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037705595"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-3-0348-0364-9_1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038460386", 
          "https://doi.org/10.1007/978-3-0348-0364-9_1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/mop.0b013e328334581f", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043370235"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/mop.0b013e328334581f", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043370235"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/mop.0b013e328334581f", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043370235"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.semcdb.2015.09.017", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043787283"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.21873/anticanres.10982", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1069267025"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2478/v10039-012-0020-z", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1070852162"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3892/ijo.32.4.757", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1071516809"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3892/or_00000885", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1071537669"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1158/0008-5472.can-04-2218", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1077039467"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078355475", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079193404", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2018-10", 
    "datePublishedReg": "2018-10-01", 
    "description": "Osteosarcoma is the most common primary malignant bone tumor in childhood, and it maintains a high level of recurrence. Matrix metalloproteinase-1 (MMP-1) was found to contribute to cancer progression. The present study was to investigate the in vitro effects of MMP-1 over-expression on the proliferation, invasion, metastasis and stem-like properties of osteosarcoma MG-63 cells. The MG-63 cells were cultured and had a full length MMP-1 cDNA inserted by the lentiviral vector (MG-63MMP-1+). MG-63 negative control and MG-63 blank control groups were established as well. MMP-1 expression was detected in MG-63MMP-1+, MG-63 negative control and MG-63 blank control cells using qPCR, Western blotting and immunofluorescence after 24 h of culture. The cell proliferation assay was performed with a camera attached to a bioreactor, which was programmed to photograph five regions of each well every 10 min over a period of 48 h. The cell invasion assay was conducted with Matrigel to assess the invasive potential of MG-63 cells over 24 h, the qPCR analysis to measure stem cell markers, including Oct4, Sox-2, Nanog, and Pax-7, and Western blot analysis to detect invasive and metastatic potential markers TIMP-1, VEGF and BMP2/4, after 24 h of culture. Immunofluorescence was used to investigate the presence of the stem cell marker Pax-7 after 24-h culture. The results showed that over-expression of MMP-1 after transfection could significantly increase tumor cell proliferation and invasion (P<0.05, MG-63MMP-1+versus controls). Pax-7 was highly expressed in MG-63MMP-1+ cells, with no significant changes of Oct-4, Sox-2, and Nanog observed (P<0.05). MG-63MMP-1+ cells showed higher expression of VEGF and BMP 2/4 proteins and lower expression of TIMP-1 protein than controls (P<0.05). It was concluded that MMP-1 over-expression in MG-63 cells contributed to the proliferation, invasion, metastasis and stem-like properties of osteosarcoma cells. Future studies should focus on in vivo effects of MMP-1 over-expression and the application of MMP-1 and Pax-7 inhibition in vivo to osteosarcoma therapies.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s11596-018-1947-5", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1032019", 
        "issn": [
          "2096-5230", 
          "2523-899X"
        ], 
        "name": "ACTA ACADEMIAE MEDICINAE WUHAN", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "5", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "38"
      }
    ], 
    "name": "MMP-1 Over-expression Promotes Malignancy and Stem-Like Properties of Human Osteosarcoma MG-63 Cells In Vitro", 
    "pagination": "809-817", 
    "productId": [
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s11596-018-1947-5"
        ]
      }, 
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "188ed8f4253a55d0768833b746180aad513ff3fdb02da12764984fbf92eb9fd1"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1110953299"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101729993"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "30594980"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s11596-018-1947-5", 
      "https://app.dimensions.ai/details/publication/pub.1110953299"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-15T09:20", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000376_0000000376/records_56179_00000006.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://link.springer.com/10.1007%2Fs11596-018-1947-5"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s11596-018-1947-5'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s11596-018-1947-5'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s11596-018-1947-5'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s11596-018-1947-5'


 

This table displays all metadata directly associated to this object as RDF triples.

244 TRIPLES      21 PREDICATES      72 URIs      31 LITERALS      19 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s11596-018-1947-5 schema:about N00184d81c847457e95f2c233407824b4
2 N15870be2fcbc4d5299e9303d73907970
3 N2ddd028d85cc41a49f5ab1e4ceabea8a
4 N438198b64cd5428fa24f5f805d24427b
5 N5a3ff9c04a164d07ba9cac5a72d65363
6 Na82d99c543a448968a1c57494c202e3c
7 Nca27a20bcec34f098b819bce4acbea56
8 Ndac653e99f0544689d24285c82ed5fee
9 Ndbf139e0ffb647cbbc336b39c9094f6c
10 Nee372c28e7fa4a20be375c410c08d761
11 anzsrc-for:11
12 anzsrc-for:1112
13 schema:author N68f31cdbcfea421da6e3878e8a0c7c9e
14 schema:citation sg:pub.10.1007/978-3-0348-0364-9_1
15 sg:pub.10.1007/978-3-319-04843-7_11
16 sg:pub.10.1007/s13277-013-0842-8
17 sg:pub.10.1038/nrc3838
18 https://app.dimensions.ai/details/publication/pub.1078355475
19 https://app.dimensions.ai/details/publication/pub.1079193404
20 https://doi.org/10.1002/cncr.24519
21 https://doi.org/10.1006/scbi.2000.0379
22 https://doi.org/10.1016/j.bbadis.2012.11.006
23 https://doi.org/10.1016/j.bbamcr.2011.04.008
24 https://doi.org/10.1016/j.biochi.2004.09.022
25 https://doi.org/10.1016/j.cell.2010.03.015
26 https://doi.org/10.1016/j.matbio.2015.01.019
27 https://doi.org/10.1016/j.matbio.2015.01.022
28 https://doi.org/10.1016/j.semcdb.2015.09.017
29 https://doi.org/10.1016/j.ydbio.2004.08.015
30 https://doi.org/10.1093/mutage/geu045
31 https://doi.org/10.1097/01.blo.0000229333.98781.56
32 https://doi.org/10.1097/mop.0b013e328334581f
33 https://doi.org/10.1111/jmi.12064
34 https://doi.org/10.1158/0008-5472.can-04-2218
35 https://doi.org/10.1158/0008-5472.can-08-3580
36 https://doi.org/10.1371/journal.pone.0098549
37 https://doi.org/10.15252/embr.201439246
38 https://doi.org/10.18632/oncotarget.4902
39 https://doi.org/10.2147/ott.s84561
40 https://doi.org/10.21873/anticanres.10982
41 https://doi.org/10.2353/ajpath.2009.080509
42 https://doi.org/10.2478/v10039-012-0020-z
43 https://doi.org/10.3389/fgene.2012.00006
44 https://doi.org/10.3892/ijo.32.4.757
45 https://doi.org/10.3892/or_00000885
46 https://doi.org/10.7314/apjcp.2014.15.3.1085
47 schema:datePublished 2018-10
48 schema:datePublishedReg 2018-10-01
49 schema:description Osteosarcoma is the most common primary malignant bone tumor in childhood, and it maintains a high level of recurrence. Matrix metalloproteinase-1 (MMP-1) was found to contribute to cancer progression. The present study was to investigate the in vitro effects of MMP-1 over-expression on the proliferation, invasion, metastasis and stem-like properties of osteosarcoma MG-63 cells. The MG-63 cells were cultured and had a full length MMP-1 cDNA inserted by the lentiviral vector (MG-63<sup>MMP-1+</sup>). MG-63 negative control and MG-63 blank control groups were established as well. MMP-1 expression was detected in MG-63<sup>MMP-1+</sup>, MG-63 negative control and MG-63 blank control cells using qPCR, Western blotting and immunofluorescence after 24 h of culture. The cell proliferation assay was performed with a camera attached to a bioreactor, which was programmed to photograph five regions of each well every 10 min over a period of 48 h. The cell invasion assay was conducted with Matrigel to assess the invasive potential of MG-63 cells over 24 h, the qPCR analysis to measure stem cell markers, including Oct4, Sox-2, Nanog, and Pax-7, and Western blot analysis to detect invasive and metastatic potential markers TIMP-1, VEGF and BMP2/4, after 24 h of culture. Immunofluorescence was used to investigate the presence of the stem cell marker Pax-7 after 24-h culture. The results showed that over-expression of MMP-1 after transfection could significantly increase tumor cell proliferation and invasion (P&lt;0.05, MG-63<sup>MMP-1+</sup>versus controls). Pax-7 was highly expressed in MG-63<sup>MMP-1+</sup> cells, with no significant changes of Oct-4, Sox-2, and Nanog observed (P&lt;0.05). MG-63<sup>MMP-1+</sup> cells showed higher expression of VEGF and BMP 2/4 proteins and lower expression of TIMP-1 protein than controls (P&lt;0.05). It was concluded that MMP-1 over-expression in MG-63 cells contributed to the proliferation, invasion, metastasis and stem-like properties of osteosarcoma cells. Future studies should focus on in vivo effects of MMP-1 over-expression and the application of MMP-1 and Pax-7 inhibition in vivo to osteosarcoma therapies.
50 schema:genre research_article
51 schema:inLanguage en
52 schema:isAccessibleForFree false
53 schema:isPartOf N733ba41a269449be9e5a76ea86f0bf17
54 N9fc48dfdacdb4077a1ddcff7850a6e5f
55 sg:journal.1032019
56 schema:name MMP-1 Over-expression Promotes Malignancy and Stem-Like Properties of Human Osteosarcoma MG-63 Cells In Vitro
57 schema:pagination 809-817
58 schema:productId Nb19093af7dbb4ff1a5f97d162514c640
59 Nc3895988a8e240f0ae3d8635757a3a33
60 Nc6426b7aef4440eb953701b73cfdbce1
61 Nddcb02c4cbe04670a26cb446e4682130
62 Nf1f8fc6d05bf41a4865f00ac5b1c8012
63 schema:sameAs https://app.dimensions.ai/details/publication/pub.1110953299
64 https://doi.org/10.1007/s11596-018-1947-5
65 schema:sdDatePublished 2019-04-15T09:20
66 schema:sdLicense https://scigraph.springernature.com/explorer/license/
67 schema:sdPublisher N26a6d151346d43859a6cfc2764655f76
68 schema:url https://link.springer.com/10.1007%2Fs11596-018-1947-5
69 sgo:license sg:explorer/license/
70 sgo:sdDataset articles
71 rdf:type schema:ScholarlyArticle
72 N00184d81c847457e95f2c233407824b4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
73 schema:name Humans
74 rdf:type schema:DefinedTerm
75 N1191939c29cf45949c3475d6e3ba6bb3 schema:name Department of Traumatic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430030, Wuhan, China
76 rdf:type schema:Organization
77 N15870be2fcbc4d5299e9303d73907970 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
78 schema:name Neoplasm Invasiveness
79 rdf:type schema:DefinedTerm
80 N239957dcba734c089b551dde84e10220 schema:affiliation https://www.grid.ac/institutes/grid.419649.7
81 schema:familyName Li
82 schema:givenName Yong
83 rdf:type schema:Person
84 N23a702c88d13491fa9bcc8a6bf752d7b rdf:first N50539bf353aa433093a0cf7c1c50c3d6
85 rdf:rest rdf:nil
86 N26a6d151346d43859a6cfc2764655f76 schema:name Springer Nature - SN SciGraph project
87 rdf:type schema:Organization
88 N2ddd028d85cc41a49f5ab1e4ceabea8a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
89 schema:name Cell Line, Tumor
90 rdf:type schema:DefinedTerm
91 N438198b64cd5428fa24f5f805d24427b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
92 schema:name Cell Proliferation
93 rdf:type schema:DefinedTerm
94 N4b79942294214aefa2016dbcd6c6e704 schema:affiliation https://www.grid.ac/institutes/grid.266436.3
95 schema:familyName Dai
96 schema:givenName Xiao-jing
97 rdf:type schema:Person
98 N50539bf353aa433093a0cf7c1c50c3d6 schema:affiliation https://www.grid.ac/institutes/grid.419649.7
99 schema:familyName Yang
100 schema:givenName Fan
101 rdf:type schema:Person
102 N5a3ff9c04a164d07ba9cac5a72d65363 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
103 schema:name Vascular Endothelial Growth Factor A
104 rdf:type schema:DefinedTerm
105 N60299ee56fae47f9abe0a9b3dba0058f schema:name Department of Traumatic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430030, Wuhan, China
106 rdf:type schema:Organization
107 N618236d09d25418b85ffea4db18acb35 schema:affiliation N60299ee56fae47f9abe0a9b3dba0058f
108 schema:familyName Tang
109 schema:givenName Man-li
110 rdf:type schema:Person
111 N68f31cdbcfea421da6e3878e8a0c7c9e rdf:first N618236d09d25418b85ffea4db18acb35
112 rdf:rest Naf71404bd8574c41ac8e995b78e33054
113 N6e382695676648409040cc8ae7931111 rdf:first N4b79942294214aefa2016dbcd6c6e704
114 rdf:rest N23a702c88d13491fa9bcc8a6bf752d7b
115 N733ba41a269449be9e5a76ea86f0bf17 schema:volumeNumber 38
116 rdf:type schema:PublicationVolume
117 N9fc48dfdacdb4077a1ddcff7850a6e5f schema:issueNumber 5
118 rdf:type schema:PublicationIssue
119 Na82d99c543a448968a1c57494c202e3c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
120 schema:name Biomarkers, Tumor
121 rdf:type schema:DefinedTerm
122 Naf71404bd8574c41ac8e995b78e33054 rdf:first sg:person.0757422057.71
123 rdf:rest Nfd084860607341f8ad31b75c5d33175a
124 Nb19093af7dbb4ff1a5f97d162514c640 schema:name doi
125 schema:value 10.1007/s11596-018-1947-5
126 rdf:type schema:PropertyValue
127 Nc3895988a8e240f0ae3d8635757a3a33 schema:name readcube_id
128 schema:value 188ed8f4253a55d0768833b746180aad513ff3fdb02da12764984fbf92eb9fd1
129 rdf:type schema:PropertyValue
130 Nc6426b7aef4440eb953701b73cfdbce1 schema:name dimensions_id
131 schema:value pub.1110953299
132 rdf:type schema:PropertyValue
133 Nca27a20bcec34f098b819bce4acbea56 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
134 schema:name Osteosarcoma
135 rdf:type schema:DefinedTerm
136 Ndac653e99f0544689d24285c82ed5fee schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
137 schema:name Bone Neoplasms
138 rdf:type schema:DefinedTerm
139 Ndbf139e0ffb647cbbc336b39c9094f6c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
140 schema:name Tissue Inhibitor of Metalloproteinase-1
141 rdf:type schema:DefinedTerm
142 Nddcb02c4cbe04670a26cb446e4682130 schema:name pubmed_id
143 schema:value 30594980
144 rdf:type schema:PropertyValue
145 Nee372c28e7fa4a20be375c410c08d761 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
146 schema:name Matrix Metalloproteinase 1
147 rdf:type schema:DefinedTerm
148 Nf1f8fc6d05bf41a4865f00ac5b1c8012 schema:name nlm_unique_id
149 schema:value 101729993
150 rdf:type schema:PropertyValue
151 Nfd084860607341f8ad31b75c5d33175a rdf:first N239957dcba734c089b551dde84e10220
152 rdf:rest N6e382695676648409040cc8ae7931111
153 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
154 schema:name Medical and Health Sciences
155 rdf:type schema:DefinedTerm
156 anzsrc-for:1112 schema:inDefinedTermSet anzsrc-for:
157 schema:name Oncology and Carcinogenesis
158 rdf:type schema:DefinedTerm
159 sg:journal.1032019 schema:issn 2096-5230
160 2523-899X
161 schema:name ACTA ACADEMIAE MEDICINAE WUHAN
162 rdf:type schema:Periodical
163 sg:person.0757422057.71 schema:affiliation N1191939c29cf45949c3475d6e3ba6bb3
164 schema:familyName Bai
165 schema:givenName Xiang-jun
166 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0757422057.71
167 rdf:type schema:Person
168 sg:pub.10.1007/978-3-0348-0364-9_1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038460386
169 https://doi.org/10.1007/978-3-0348-0364-9_1
170 rdf:type schema:CreativeWork
171 sg:pub.10.1007/978-3-319-04843-7_11 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002069665
172 https://doi.org/10.1007/978-3-319-04843-7_11
173 rdf:type schema:CreativeWork
174 sg:pub.10.1007/s13277-013-0842-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004513159
175 https://doi.org/10.1007/s13277-013-0842-8
176 rdf:type schema:CreativeWork
177 sg:pub.10.1038/nrc3838 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032448549
178 https://doi.org/10.1038/nrc3838
179 rdf:type schema:CreativeWork
180 https://app.dimensions.ai/details/publication/pub.1078355475 schema:CreativeWork
181 https://app.dimensions.ai/details/publication/pub.1079193404 schema:CreativeWork
182 https://doi.org/10.1002/cncr.24519 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005687305
183 rdf:type schema:CreativeWork
184 https://doi.org/10.1006/scbi.2000.0379 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028271714
185 rdf:type schema:CreativeWork
186 https://doi.org/10.1016/j.bbadis.2012.11.006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029341141
187 rdf:type schema:CreativeWork
188 https://doi.org/10.1016/j.bbamcr.2011.04.008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035013562
189 rdf:type schema:CreativeWork
190 https://doi.org/10.1016/j.biochi.2004.09.022 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035621430
191 rdf:type schema:CreativeWork
192 https://doi.org/10.1016/j.cell.2010.03.015 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015080267
193 rdf:type schema:CreativeWork
194 https://doi.org/10.1016/j.matbio.2015.01.019 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012749457
195 rdf:type schema:CreativeWork
196 https://doi.org/10.1016/j.matbio.2015.01.022 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017053828
197 rdf:type schema:CreativeWork
198 https://doi.org/10.1016/j.semcdb.2015.09.017 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043787283
199 rdf:type schema:CreativeWork
200 https://doi.org/10.1016/j.ydbio.2004.08.015 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022282991
201 rdf:type schema:CreativeWork
202 https://doi.org/10.1093/mutage/geu045 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011684323
203 rdf:type schema:CreativeWork
204 https://doi.org/10.1097/01.blo.0000229333.98781.56 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009210968
205 rdf:type schema:CreativeWork
206 https://doi.org/10.1097/mop.0b013e328334581f schema:sameAs https://app.dimensions.ai/details/publication/pub.1043370235
207 rdf:type schema:CreativeWork
208 https://doi.org/10.1111/jmi.12064 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030779747
209 rdf:type schema:CreativeWork
210 https://doi.org/10.1158/0008-5472.can-04-2218 schema:sameAs https://app.dimensions.ai/details/publication/pub.1077039467
211 rdf:type schema:CreativeWork
212 https://doi.org/10.1158/0008-5472.can-08-3580 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020859407
213 rdf:type schema:CreativeWork
214 https://doi.org/10.1371/journal.pone.0098549 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021506355
215 rdf:type schema:CreativeWork
216 https://doi.org/10.15252/embr.201439246 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002160543
217 rdf:type schema:CreativeWork
218 https://doi.org/10.18632/oncotarget.4902 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037705595
219 rdf:type schema:CreativeWork
220 https://doi.org/10.2147/ott.s84561 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036265038
221 rdf:type schema:CreativeWork
222 https://doi.org/10.21873/anticanres.10982 schema:sameAs https://app.dimensions.ai/details/publication/pub.1069267025
223 rdf:type schema:CreativeWork
224 https://doi.org/10.2353/ajpath.2009.080509 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004391724
225 rdf:type schema:CreativeWork
226 https://doi.org/10.2478/v10039-012-0020-z schema:sameAs https://app.dimensions.ai/details/publication/pub.1070852162
227 rdf:type schema:CreativeWork
228 https://doi.org/10.3389/fgene.2012.00006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023813425
229 rdf:type schema:CreativeWork
230 https://doi.org/10.3892/ijo.32.4.757 schema:sameAs https://app.dimensions.ai/details/publication/pub.1071516809
231 rdf:type schema:CreativeWork
232 https://doi.org/10.3892/or_00000885 schema:sameAs https://app.dimensions.ai/details/publication/pub.1071537669
233 rdf:type schema:CreativeWork
234 https://doi.org/10.7314/apjcp.2014.15.3.1085 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015776718
235 rdf:type schema:CreativeWork
236 https://www.grid.ac/institutes/grid.266436.3 schema:alternateName University of Houston
237 schema:name Department of Pharmacology and Toxicology, College of Pharmacy, the University of Houston, 77555, Houston, USA
238 rdf:type schema:Organization
239 https://www.grid.ac/institutes/grid.419649.7 schema:alternateName Steadman Philippon Research Institute
240 schema:name Center for Regenerative Sports Medicine, Steadman Philippon Research Institute, 81657, Vail, USA
241 Center for Stem Cell and Regenerative Medicine, UTHealth, 77035, Houston, USA
242 Department of Orthopaedic Surgery, McGovern Medical School, the University of Texas Health Science Center at Houston (UTHealth), 77035, Houston, USA
243 Department of Traumatic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430030, Wuhan, China
244 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...