Fingerprinting of Potato Genotypes from Estonian Genebank Collection Using SSR Markers View Full Text


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Article Info

DATE

2021-07-08

AUTHORS

Anna Ivanova-Pozdejeva, Agnes Kivistik, Liisa Kübarsepp, Terje Tähtjärv, Aide Tsahkna, Eric Droz, Kristiina Laanemets

ABSTRACT

Over 450 potato varieties and landraces are preserved as in vitro cultures by the Plant Biotechnology department of Estonian Crop Research Institute. This collection contains germplasm from various countries as well as old Estonian landraces. These potato accessions are periodically grown in the field for verification; however, this is not always sufficient to validate varieties and, in many cases, the true origin or identity of old landraces is not known. A reliable and cost-effective method for fingerprinting Estonian potato germplasm needed to be implemented. Simple sequence repeat (SSR) markers are known as robust and efficient markers for the identification of tetraploid potato. Here, fingerprinting results are presented for 103 genotypes from the Estonian potato collection using 8 SSR markers. It was shown that as few as three markers were sufficient to yield unique fingerprints for the given set of samples. SSR markers were used to estimate the allele copy number with varying success. Additionally, the reproducibility of four of these markers was assessed in two different laboratories, and repeatable results were observed. It was verified that the putative landrace ‘Kuldkera’ has the same genetic fingerprint as the variety ‘Ando’, whilst an expected clone of ‘Early Rose’ is a completely different genotype. Implementation of genetic fingerprinting and these results is of high importance for further potato genebank management and Estonian breeding programme. More... »

PAGES

1-18

References to SciGraph publications

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  • 2005-06. Potato cultivar identification using simple sequence repeats markers (SSR) in POTATO RESEARCH
  • 2010-06-27. Genetic relationships among local potato cultivars from Spain using SSR markers in GENETIC RESOURCES AND CROP EVOLUTION
  • 2005-06-08. Mapping and characterization of new EST-derived microsatellites for potato (Solanum tuberosum L.) in THEORETICAL AND APPLIED GENETICS
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  • 2003-12-17. Microsatellite DNA marker inheritance indicates preferential pairing between two highly homologous genomes in polyploid and hemisexual dog-roses, Rosa L. Sect. Caninae DC. in HEREDITY
  • 2016-11-14. Genetic Fingerprinting of Potato Varieties from the Northwest Potato Variety Development Program in AMERICAN JOURNAL OF POTATO RESEARCH
  • 2017-05-31. A System for Identification of Potato Varieties Using SNP Dosage in AMERICAN JOURNAL OF POTATO RESEARCH
  • 2004-04-14. Assignment of allelic configuration in polyploids using the MAC-PR (microsatellite DNA allele counting—peak ratios) method in THEORETICAL AND APPLIED GENETICS
  • 2013-09-04. Molecular Analysis of Potatoes from the Pacific Northwest Tri-State Variety Development Program and Selection of Markers for Practical DNA Fingerprinting Applications in AMERICAN JOURNAL OF POTATO RESEARCH
  • 2013-05-31. Evaluation of Simple Sequence Repeat (SSR) Markers Established in Europe as a Method for the Identification of Potato Varieties Grown in Canada in AMERICAN JOURNAL OF POTATO RESEARCH
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    27 schema:description Over 450 potato varieties and landraces are preserved as in vitro cultures by the Plant Biotechnology department of Estonian Crop Research Institute. This collection contains germplasm from various countries as well as old Estonian landraces. These potato accessions are periodically grown in the field for verification; however, this is not always sufficient to validate varieties and, in many cases, the true origin or identity of old landraces is not known. A reliable and cost-effective method for fingerprinting Estonian potato germplasm needed to be implemented. Simple sequence repeat (SSR) markers are known as robust and efficient markers for the identification of tetraploid potato. Here, fingerprinting results are presented for 103 genotypes from the Estonian potato collection using 8 SSR markers. It was shown that as few as three markers were sufficient to yield unique fingerprints for the given set of samples. SSR markers were used to estimate the allele copy number with varying success. Additionally, the reproducibility of four of these markers was assessed in two different laboratories, and repeatable results were observed. It was verified that the putative landrace ‘Kuldkera’ has the same genetic fingerprint as the variety ‘Ando’, whilst an expected clone of ‘Early Rose’ is a completely different genotype. Implementation of genetic fingerprinting and these results is of high importance for further potato genebank management and Estonian breeding programme.
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    33 Biotechnology Department
    34 Crops Research Institute
    35 Department
    36 Early Rose
    37 Estonian Crop Research Institute
    38 Estonian Genebank Collection
    39 Estonian breeding programme
    40 Estonian landraces
    41 Estonian potato collection
    42 Estonian potato germplasm
    43 Institute
    44 Kuldkera
    45 Plant Biotechnology department
    46 Research Institute
    47 Rose
    48 SSR markers
    49 accessions
    50 allele copy number
    51 breeding programs
    52 cases
    53 clones
    54 collection
    55 copy number
    56 cost-effective method
    57 countries
    58 culture
    59 different genotypes
    60 different laboratories
    61 efficient marker
    62 field
    63 fingerprinting
    64 fingerprinting results
    65 fingerprints
    66 further potato genebank management
    67 genebank collections
    68 genebank management
    69 genetic fingerprinting
    70 genetic fingerprints
    71 genotypes
    72 germplasm
    73 high importance
    74 identification
    75 identity
    76 implementation
    77 importance
    78 laboratory
    79 landraces
    80 management
    81 markers
    82 method
    83 number
    84 old Estonian landraces
    85 old landraces
    86 origin
    87 potato
    88 potato accessions
    89 potato collection
    90 potato genebank management
    91 potato genotypes
    92 potato germplasm
    93 potato varieties
    94 program
    95 putative landrace
    96 repeat markers
    97 repeatable results
    98 reproducibility
    99 results
    100 same genetic fingerprint
    101 samples
    102 sequence repeat markers
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    105 simple sequence repeat (SSR) markers
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    107 tetraploid potato
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    112 schema:name Fingerprinting of Potato Genotypes from Estonian Genebank Collection Using SSR Markers
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