Durable Late Blight Resistance in Potato Through Dynamic Varieties Obtained by Cisgenesis: Scientific and Societal Advances in the DuRPh Project View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2016-03

AUTHORS

A. J. Haverkort, P. M. Boonekamp, R. Hutten, E. Jacobsen, L. A. P. Lotz, G. J. T. Kessel, J. H. Vossen, R. G. F. Visser

ABSTRACT

From 2006 through 2015, a research project on Durable Resistance in potato against Phytophthora (DuRPh) was carried out at Wageningen University and Research Centre. Its objective was to develop a proof of principle for durable resistance against late blight by cisgenesis. This public-funded project aimed at stimulating research on genetic modification and public debate on innovative genetic techniques. It was decided to clone and transfer late blight resistance (R) genes of crossable wild potato species (cisgenes) by Agrobacterium tumefaciens-mediated transformation without non-potato genes. A stack of multiple R genes were planned to be inserted into established varieties, thereby creating a dynamic variety in which the composition of the stacks may vary over space and time. Cisgenic plants were selected based on the expression of all inserted R genes and trueness-to-type. Within the project, 13 R genes from wild potato species were genetically mapped and three of them were cloned. Four varieties were transformed with one to three R genes. This was initially done using kanamycin resistance provided by a selectable marker gene of synthetic origin in order to quickly test the performance and stability of the introduced R genes and stacked R gene combinations. Once the functioning thereof was confirmed, marker-free transformations were conducted; thus, true cisgenic events were selected. The results about the different R genes, their chromosomal location, their specificity, the background dependence, the maximum size of a stack, its regeneration time and associated somaclonal variation frequency and its stability were studied. After selection and characterisation in the laboratory, the best cisgenic events were assessed in field trials for late blight resistance. This showed that inserted R genes were capable of turning a susceptible variety into a resistant one. Maximising longevity of the resistance was assured through resistance management research. It was shown that stacking of multiple R genes and monitoring how to deploy these stacks spatially and temporally could reduce fungicide use by over 80%. Communications through media and field demonstrations were manifold to allow public and policymakers to decide if cisgenesis is an acceptable tool to make potato farming more sustainable. Future deployment of the DuRPh strategy will depend largely on its status as a genetically modified crop or its exemption thereof. Worldwide near eradication of late blight would increase global annual potato production by close to 80 million tons, thereby contributing considerably to the needed additional global future food supply. More... »

PAGES

35-66

References to SciGraph publications

  • 2006-12. Common infection strategies of pathogenic eukaryotes in NATURE REVIEWS MICROBIOLOGY
  • 2008-03. Societal Costs of Late Blight in Potato and Prospects of Durable Resistance Through Cisgenic Modification in POTATO RESEARCH
  • 2011-12. Mapping of the S. demissum late blight resistance gene R8 to a new locus on chromosome IX in THEORETICAL AND APPLIED GENETICS
  • 2014-12. Development of late blight resistant potatoes by cisgene stacking in BMC BIOTECHNOLOGY
  • 2008-05. Allele mining in Solanum: conserved homologues of Rpi-blb1 are identified in Solanum stoloniferum in THEORETICAL AND APPLIED GENETICS
  • 2013-12. Novel applications of motif-directed profiling to identify disease resistance genes in plants in PLANT METHODS
  • 2013-04. Vector integration in triple R gene transformants and the clustered inheritance of resistance against potato late blight in TRANSGENIC RESEARCH
  • 2012-03. Broad spectrum late blight resistance in potato differential set plants MaR8 and MaR9 is conferred by multiple stacked R genes in THEORETICAL AND APPLIED GENETICS
  • 1999-06. Foliar Aggressiveness of Phytophthora infestans in Three Potato Growing Regions in the Netherlands in EUROPEAN JOURNAL OF PLANT PATHOLOGY
  • 2003-04. A transformation method for obtaining marker-free plants of a cross-pollinating and vegetatively propagated crop in NATURE BIOTECHNOLOGY
  • 2011-12. Cool Farm Tool – Potato: Model Description and Performance of Four Production Systems in POTATO RESEARCH
  • 2015-03. An updated conventional- and a novel GM potato late blight R gene differential set for virulence monitoring of Phytophthora infestans in EUPHYTICA
  • 2015-05. Characterisation of the late blight resistance in potato differential MaR9 reveals a qualitative resistance gene, R9a, residing in a cluster of Tm-22 homologs on chromosome IX in THEORETICAL AND APPLIED GENETICS
  • 2009-08. Applied Biotechnology to Combat Late Blight in Potato Caused by Phytophthora Infestans in POTATO RESEARCH
  • 2008. The Present and Future Role of Insect-Resistant Genetically Modified Potato Cultivars in IPM in INTEGRATION OF INSECT-RESISTANT GENETICALLY MODIFIED CROPS WITHIN IPM PROGRAMS
  • 1953-10. A proposal for an international nomenclature of races of Phytophthora infestans and of genes controlling immunity in Solanum demissum derivatives in EUPHYTICA
  • 2011-07-10. Genome sequence and analysis of the tuber crop potato in NATURE
  • 2009-09. Genome sequence and analysis of the Irish potato famine pathogen Phytophthora infestans in NATURE
  • 2005-01. The spores of Phytophthora: weapons of the plant destroyer in NATURE REVIEWS MICROBIOLOGY
  • Journal

    TITLE

    Potato Research

    ISSUE

    1

    VOLUME

    59

    Author Affiliations

    Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s11540-015-9312-6

    DOI

    http://dx.doi.org/10.1007/s11540-015-9312-6

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1033975255


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Wageningen University & Research", 
              "id": "https://www.grid.ac/institutes/grid.4818.5", 
              "name": [
                "Wageningen University and Research Centre, P.O. Box 16, 6700 AA, Wageningen, The Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Haverkort", 
            "givenName": "A. J.", 
            "id": "sg:person.016711451021.37", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016711451021.37"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Wageningen University & Research", 
              "id": "https://www.grid.ac/institutes/grid.4818.5", 
              "name": [
                "Wageningen University and Research Centre, P.O. Box 16, 6700 AA, Wageningen, The Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Boonekamp", 
            "givenName": "P. M.", 
            "id": "sg:person.012135321063.85", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012135321063.85"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Wageningen University & Research", 
              "id": "https://www.grid.ac/institutes/grid.4818.5", 
              "name": [
                "Wageningen University and Research Centre, P.O. Box 16, 6700 AA, Wageningen, The Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Hutten", 
            "givenName": "R.", 
            "id": "sg:person.012547223026.00", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012547223026.00"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Wageningen University & Research", 
              "id": "https://www.grid.ac/institutes/grid.4818.5", 
              "name": [
                "Wageningen University and Research Centre, P.O. Box 16, 6700 AA, Wageningen, The Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Jacobsen", 
            "givenName": "E.", 
            "id": "sg:person.01240634504.23", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01240634504.23"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Wageningen University & Research", 
              "id": "https://www.grid.ac/institutes/grid.4818.5", 
              "name": [
                "Wageningen University and Research Centre, P.O. Box 16, 6700 AA, Wageningen, The Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Lotz", 
            "givenName": "L. A. P.", 
            "id": "sg:person.01273472052.71", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01273472052.71"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Wageningen University & Research", 
              "id": "https://www.grid.ac/institutes/grid.4818.5", 
              "name": [
                "Wageningen University and Research Centre, P.O. Box 16, 6700 AA, Wageningen, The Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kessel", 
            "givenName": "G. J. T.", 
            "id": "sg:person.014000776505.64", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014000776505.64"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Wageningen University & Research", 
              "id": "https://www.grid.ac/institutes/grid.4818.5", 
              "name": [
                "Wageningen University and Research Centre, P.O. Box 16, 6700 AA, Wageningen, The Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Vossen", 
            "givenName": "J. H.", 
            "id": "sg:person.01121244246.75", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01121244246.75"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Wageningen University & Research", 
              "id": "https://www.grid.ac/institutes/grid.4818.5", 
              "name": [
                "Wageningen University and Research Centre, P.O. Box 16, 6700 AA, Wageningen, The Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Visser", 
            "givenName": "R. G. F.", 
            "id": "sg:person.011217761732.44", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011217761732.44"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1111/mpp.12036", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000809331"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00122-008-0725-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002289659", 
              "https://doi.org/10.1007/s00122-008-0725-3"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature10158", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002525491", 
              "https://doi.org/10.1038/nature10158"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.cropro.2015.07.018", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003053464"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-1-4020-8373-0_7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003323709", 
              "https://doi.org/10.1007/978-1-4020-8373-0_7"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00122-011-1670-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005196195", 
              "https://doi.org/10.1007/s00122-011-1670-0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrmicro1064", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007773140", 
              "https://doi.org/10.1038/nrmicro1064"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrmicro1064", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007773140", 
              "https://doi.org/10.1038/nrmicro1064"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrmicro1064", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007773140", 
              "https://doi.org/10.1038/nrmicro1064"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.njas.2014.05.004", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008128603"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1080/09670879309371781", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008997861"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00122-015-2480-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009677098", 
              "https://doi.org/10.1007/s00122-015-2480-6"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00122-015-2480-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009677098", 
              "https://doi.org/10.1007/s00122-015-2480-6"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1365-3059.2007.01571.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010613094"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s10681-014-1276-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012062182", 
              "https://doi.org/10.1007/s10681-014-1276-0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s10681-014-1276-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012062182", 
              "https://doi.org/10.1007/s10681-014-1276-0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1472-6750-14-50", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016051380", 
              "https://doi.org/10.1186/1472-6750-14-50"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11540-009-9136-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018769800", 
              "https://doi.org/10.1007/s11540-009-9136-3"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11540-009-9136-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018769800", 
              "https://doi.org/10.1007/s11540-009-9136-3"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11540-011-9194-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024234848", 
              "https://doi.org/10.1007/s11540-011-9194-1"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1746-4811-9-37", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025042771", 
              "https://doi.org/10.1186/1746-4811-9-37"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1038/sj.embor.7400769", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025213095"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature08358", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025806080", 
              "https://doi.org/10.1038/nature08358"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature08358", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025806080", 
              "https://doi.org/10.1038/nature08358"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrmicro1549", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028588894", 
              "https://doi.org/10.1038/nrmicro1549"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrmicro1549", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028588894", 
              "https://doi.org/10.1038/nrmicro1549"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00053724", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030721382", 
              "https://doi.org/10.1007/bf00053724"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00053724", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030721382", 
              "https://doi.org/10.1007/bf00053724"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/tpj.12307", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037179492"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11248-012-9644-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037762381", 
              "https://doi.org/10.1007/s11248-012-9644-9"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt801", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042004278", 
              "https://doi.org/10.1038/nbt801"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt801", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042004278", 
              "https://doi.org/10.1038/nbt801"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1023/a:1008737222830", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042144868", 
              "https://doi.org/10.1023/a:1008737222830"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1365-2338.1991.tb00443.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043759572"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11540-008-9089-y", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049487093", 
              "https://doi.org/10.1007/s11540-008-9089-y"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.ppat.1002940", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051265373"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00122-011-1757-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1052258489", 
              "https://doi.org/10.1007/s00122-011-1757-7"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1146/annurev-phyto-072910-095326", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053350924"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1365-3059.2006.01491.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053722529"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1094/mpmi-01-12-0010-r", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1060077348"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1094/mpmi-11-10-0276", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1060078082"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1094/phyto-06-09-0148", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1060099249"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1094/phyto-95-0328", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1060100046"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1094/phyto-67-415", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1060105130"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1094/phyto-78-794", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1060108437"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.2307/2529674", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1069975294"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2016-03", 
        "datePublishedReg": "2016-03-01", 
        "description": "From 2006 through 2015, a research project on Durable Resistance in potato against Phytophthora (DuRPh) was carried out at Wageningen University and Research Centre. Its objective was to develop a proof of principle for durable resistance against late blight by cisgenesis. This public-funded project aimed at stimulating research on genetic modification and public debate on innovative genetic techniques. It was decided to clone and transfer late blight resistance (R) genes of crossable wild potato species (cisgenes) by Agrobacterium tumefaciens-mediated transformation without non-potato genes. A stack of multiple R genes were planned to be inserted into established varieties, thereby creating a dynamic variety in which the composition of the stacks may vary over space and time. Cisgenic plants were selected based on the expression of all inserted R genes and trueness-to-type. Within the project, 13 R genes from wild potato species were genetically mapped and three of them were cloned. Four varieties were transformed with one to three R genes. This was initially done using kanamycin resistance provided by a selectable marker gene of synthetic origin in order to quickly test the performance and stability of the introduced R genes and stacked R gene combinations. Once the functioning thereof was confirmed, marker-free transformations were conducted; thus, true cisgenic events were selected. The results about the different R genes, their chromosomal location, their specificity, the background dependence, the maximum size of a stack, its regeneration time and associated somaclonal variation frequency and its stability were studied. After selection and characterisation in the laboratory, the best cisgenic events were assessed in field trials for late blight resistance. This showed that inserted R genes were capable of turning a susceptible variety into a resistant one. Maximising longevity of the resistance was assured through resistance management research. It was shown that stacking of multiple R genes and monitoring how to deploy these stacks spatially and temporally could reduce fungicide use by over 80%. Communications through media and field demonstrations were manifold to allow public and policymakers to decide if cisgenesis is an acceptable tool to make potato farming more sustainable. Future deployment of the DuRPh strategy will depend largely on its status as a genetically modified crop or its exemption thereof. Worldwide near eradication of late blight would increase global annual potato production by close to 80 million tons, thereby contributing considerably to the needed additional global future food supply.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1007/s11540-015-9312-6", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": true, 
        "isPartOf": [
          {
            "id": "sg:journal.1030476", 
            "issn": [
              "0014-3065", 
              "1871-4528"
            ], 
            "name": "Potato Research", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "59"
          }
        ], 
        "name": "Durable Late Blight Resistance in Potato Through Dynamic Varieties Obtained by Cisgenesis: Scientific and Societal Advances in the DuRPh Project", 
        "pagination": "35-66", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "a1d9a4006c55d3cdc7507cee8e3aeffda553a7bbf0c619263251808c58faa49e"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/s11540-015-9312-6"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1033975255"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/s11540-015-9312-6", 
          "https://app.dimensions.ai/details/publication/pub.1033975255"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-10T13:20", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8659_00000523.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://link.springer.com/10.1007%2Fs11540-015-9312-6"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s11540-015-9312-6'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s11540-015-9312-6'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s11540-015-9312-6'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s11540-015-9312-6'


     

    This table displays all metadata directly associated to this object as RDF triples.

    240 TRIPLES      21 PREDICATES      64 URIs      19 LITERALS      7 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/s11540-015-9312-6 schema:about anzsrc-for:06
    2 anzsrc-for:0604
    3 schema:author N317fcbbe5cf044ecacf7e447d601a932
    4 schema:citation sg:pub.10.1007/978-1-4020-8373-0_7
    5 sg:pub.10.1007/bf00053724
    6 sg:pub.10.1007/s00122-008-0725-3
    7 sg:pub.10.1007/s00122-011-1670-0
    8 sg:pub.10.1007/s00122-011-1757-7
    9 sg:pub.10.1007/s00122-015-2480-6
    10 sg:pub.10.1007/s10681-014-1276-0
    11 sg:pub.10.1007/s11248-012-9644-9
    12 sg:pub.10.1007/s11540-008-9089-y
    13 sg:pub.10.1007/s11540-009-9136-3
    14 sg:pub.10.1007/s11540-011-9194-1
    15 sg:pub.10.1023/a:1008737222830
    16 sg:pub.10.1038/nature08358
    17 sg:pub.10.1038/nature10158
    18 sg:pub.10.1038/nbt801
    19 sg:pub.10.1038/nrmicro1064
    20 sg:pub.10.1038/nrmicro1549
    21 sg:pub.10.1186/1472-6750-14-50
    22 sg:pub.10.1186/1746-4811-9-37
    23 https://doi.org/10.1016/j.cropro.2015.07.018
    24 https://doi.org/10.1016/j.njas.2014.05.004
    25 https://doi.org/10.1038/sj.embor.7400769
    26 https://doi.org/10.1080/09670879309371781
    27 https://doi.org/10.1094/mpmi-01-12-0010-r
    28 https://doi.org/10.1094/mpmi-11-10-0276
    29 https://doi.org/10.1094/phyto-06-09-0148
    30 https://doi.org/10.1094/phyto-67-415
    31 https://doi.org/10.1094/phyto-78-794
    32 https://doi.org/10.1094/phyto-95-0328
    33 https://doi.org/10.1111/j.1365-2338.1991.tb00443.x
    34 https://doi.org/10.1111/j.1365-3059.2006.01491.x
    35 https://doi.org/10.1111/j.1365-3059.2007.01571.x
    36 https://doi.org/10.1111/mpp.12036
    37 https://doi.org/10.1111/tpj.12307
    38 https://doi.org/10.1146/annurev-phyto-072910-095326
    39 https://doi.org/10.1371/journal.ppat.1002940
    40 https://doi.org/10.2307/2529674
    41 schema:datePublished 2016-03
    42 schema:datePublishedReg 2016-03-01
    43 schema:description From 2006 through 2015, a research project on Durable Resistance in potato against Phytophthora (DuRPh) was carried out at Wageningen University and Research Centre. Its objective was to develop a proof of principle for durable resistance against late blight by cisgenesis. This public-funded project aimed at stimulating research on genetic modification and public debate on innovative genetic techniques. It was decided to clone and transfer late blight resistance (R) genes of crossable wild potato species (cisgenes) by Agrobacterium tumefaciens-mediated transformation without non-potato genes. A stack of multiple R genes were planned to be inserted into established varieties, thereby creating a dynamic variety in which the composition of the stacks may vary over space and time. Cisgenic plants were selected based on the expression of all inserted R genes and trueness-to-type. Within the project, 13 R genes from wild potato species were genetically mapped and three of them were cloned. Four varieties were transformed with one to three R genes. This was initially done using kanamycin resistance provided by a selectable marker gene of synthetic origin in order to quickly test the performance and stability of the introduced R genes and stacked R gene combinations. Once the functioning thereof was confirmed, marker-free transformations were conducted; thus, true cisgenic events were selected. The results about the different R genes, their chromosomal location, their specificity, the background dependence, the maximum size of a stack, its regeneration time and associated somaclonal variation frequency and its stability were studied. After selection and characterisation in the laboratory, the best cisgenic events were assessed in field trials for late blight resistance. This showed that inserted R genes were capable of turning a susceptible variety into a resistant one. Maximising longevity of the resistance was assured through resistance management research. It was shown that stacking of multiple R genes and monitoring how to deploy these stacks spatially and temporally could reduce fungicide use by over 80%. Communications through media and field demonstrations were manifold to allow public and policymakers to decide if cisgenesis is an acceptable tool to make potato farming more sustainable. Future deployment of the DuRPh strategy will depend largely on its status as a genetically modified crop or its exemption thereof. Worldwide near eradication of late blight would increase global annual potato production by close to 80 million tons, thereby contributing considerably to the needed additional global future food supply.
    44 schema:genre research_article
    45 schema:inLanguage en
    46 schema:isAccessibleForFree true
    47 schema:isPartOf N618f85a83aca44ef9d4c787cd835979a
    48 Nc27b5171ccf6413c9e464049f04882f3
    49 sg:journal.1030476
    50 schema:name Durable Late Blight Resistance in Potato Through Dynamic Varieties Obtained by Cisgenesis: Scientific and Societal Advances in the DuRPh Project
    51 schema:pagination 35-66
    52 schema:productId N023c244263e548b9848dc15c732d8e65
    53 N209be8973a344a909c739013f53d67e2
    54 N687dd62ba77d4145a1e25fc035fa6d93
    55 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033975255
    56 https://doi.org/10.1007/s11540-015-9312-6
    57 schema:sdDatePublished 2019-04-10T13:20
    58 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    59 schema:sdPublisher N66b1082b02094dc5807877e52cbeb507
    60 schema:url http://link.springer.com/10.1007%2Fs11540-015-9312-6
    61 sgo:license sg:explorer/license/
    62 sgo:sdDataset articles
    63 rdf:type schema:ScholarlyArticle
    64 N023c244263e548b9848dc15c732d8e65 schema:name readcube_id
    65 schema:value a1d9a4006c55d3cdc7507cee8e3aeffda553a7bbf0c619263251808c58faa49e
    66 rdf:type schema:PropertyValue
    67 N209be8973a344a909c739013f53d67e2 schema:name doi
    68 schema:value 10.1007/s11540-015-9312-6
    69 rdf:type schema:PropertyValue
    70 N2e3b283b8a2147a5b5f8cf26589bdc4f rdf:first sg:person.01240634504.23
    71 rdf:rest Ne96c10804ef447dd860dc0565a8b2c0b
    72 N317fcbbe5cf044ecacf7e447d601a932 rdf:first sg:person.016711451021.37
    73 rdf:rest N6df5b077cb2d4eacb61cd677bfaaa9a3
    74 N34887ebb83274d5abc1db19e0bb4067e rdf:first sg:person.011217761732.44
    75 rdf:rest rdf:nil
    76 N3f24476213734b1188ac5d97d3d3637d rdf:first sg:person.012547223026.00
    77 rdf:rest N2e3b283b8a2147a5b5f8cf26589bdc4f
    78 N618f85a83aca44ef9d4c787cd835979a schema:volumeNumber 59
    79 rdf:type schema:PublicationVolume
    80 N62ce1b6cfd7e4186ae2c39a46267b05e rdf:first sg:person.014000776505.64
    81 rdf:rest Na79f909bd6c8469c853692f8f473f85f
    82 N66b1082b02094dc5807877e52cbeb507 schema:name Springer Nature - SN SciGraph project
    83 rdf:type schema:Organization
    84 N687dd62ba77d4145a1e25fc035fa6d93 schema:name dimensions_id
    85 schema:value pub.1033975255
    86 rdf:type schema:PropertyValue
    87 N6df5b077cb2d4eacb61cd677bfaaa9a3 rdf:first sg:person.012135321063.85
    88 rdf:rest N3f24476213734b1188ac5d97d3d3637d
    89 Na79f909bd6c8469c853692f8f473f85f rdf:first sg:person.01121244246.75
    90 rdf:rest N34887ebb83274d5abc1db19e0bb4067e
    91 Nc27b5171ccf6413c9e464049f04882f3 schema:issueNumber 1
    92 rdf:type schema:PublicationIssue
    93 Ne96c10804ef447dd860dc0565a8b2c0b rdf:first sg:person.01273472052.71
    94 rdf:rest N62ce1b6cfd7e4186ae2c39a46267b05e
    95 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    96 schema:name Biological Sciences
    97 rdf:type schema:DefinedTerm
    98 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    99 schema:name Genetics
    100 rdf:type schema:DefinedTerm
    101 sg:journal.1030476 schema:issn 0014-3065
    102 1871-4528
    103 schema:name Potato Research
    104 rdf:type schema:Periodical
    105 sg:person.01121244246.75 schema:affiliation https://www.grid.ac/institutes/grid.4818.5
    106 schema:familyName Vossen
    107 schema:givenName J. H.
    108 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01121244246.75
    109 rdf:type schema:Person
    110 sg:person.011217761732.44 schema:affiliation https://www.grid.ac/institutes/grid.4818.5
    111 schema:familyName Visser
    112 schema:givenName R. G. F.
    113 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011217761732.44
    114 rdf:type schema:Person
    115 sg:person.012135321063.85 schema:affiliation https://www.grid.ac/institutes/grid.4818.5
    116 schema:familyName Boonekamp
    117 schema:givenName P. M.
    118 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012135321063.85
    119 rdf:type schema:Person
    120 sg:person.01240634504.23 schema:affiliation https://www.grid.ac/institutes/grid.4818.5
    121 schema:familyName Jacobsen
    122 schema:givenName E.
    123 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01240634504.23
    124 rdf:type schema:Person
    125 sg:person.012547223026.00 schema:affiliation https://www.grid.ac/institutes/grid.4818.5
    126 schema:familyName Hutten
    127 schema:givenName R.
    128 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012547223026.00
    129 rdf:type schema:Person
    130 sg:person.01273472052.71 schema:affiliation https://www.grid.ac/institutes/grid.4818.5
    131 schema:familyName Lotz
    132 schema:givenName L. A. P.
    133 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01273472052.71
    134 rdf:type schema:Person
    135 sg:person.014000776505.64 schema:affiliation https://www.grid.ac/institutes/grid.4818.5
    136 schema:familyName Kessel
    137 schema:givenName G. J. T.
    138 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014000776505.64
    139 rdf:type schema:Person
    140 sg:person.016711451021.37 schema:affiliation https://www.grid.ac/institutes/grid.4818.5
    141 schema:familyName Haverkort
    142 schema:givenName A. J.
    143 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016711451021.37
    144 rdf:type schema:Person
    145 sg:pub.10.1007/978-1-4020-8373-0_7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003323709
    146 https://doi.org/10.1007/978-1-4020-8373-0_7
    147 rdf:type schema:CreativeWork
    148 sg:pub.10.1007/bf00053724 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030721382
    149 https://doi.org/10.1007/bf00053724
    150 rdf:type schema:CreativeWork
    151 sg:pub.10.1007/s00122-008-0725-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002289659
    152 https://doi.org/10.1007/s00122-008-0725-3
    153 rdf:type schema:CreativeWork
    154 sg:pub.10.1007/s00122-011-1670-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005196195
    155 https://doi.org/10.1007/s00122-011-1670-0
    156 rdf:type schema:CreativeWork
    157 sg:pub.10.1007/s00122-011-1757-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052258489
    158 https://doi.org/10.1007/s00122-011-1757-7
    159 rdf:type schema:CreativeWork
    160 sg:pub.10.1007/s00122-015-2480-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009677098
    161 https://doi.org/10.1007/s00122-015-2480-6
    162 rdf:type schema:CreativeWork
    163 sg:pub.10.1007/s10681-014-1276-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012062182
    164 https://doi.org/10.1007/s10681-014-1276-0
    165 rdf:type schema:CreativeWork
    166 sg:pub.10.1007/s11248-012-9644-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037762381
    167 https://doi.org/10.1007/s11248-012-9644-9
    168 rdf:type schema:CreativeWork
    169 sg:pub.10.1007/s11540-008-9089-y schema:sameAs https://app.dimensions.ai/details/publication/pub.1049487093
    170 https://doi.org/10.1007/s11540-008-9089-y
    171 rdf:type schema:CreativeWork
    172 sg:pub.10.1007/s11540-009-9136-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018769800
    173 https://doi.org/10.1007/s11540-009-9136-3
    174 rdf:type schema:CreativeWork
    175 sg:pub.10.1007/s11540-011-9194-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024234848
    176 https://doi.org/10.1007/s11540-011-9194-1
    177 rdf:type schema:CreativeWork
    178 sg:pub.10.1023/a:1008737222830 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042144868
    179 https://doi.org/10.1023/a:1008737222830
    180 rdf:type schema:CreativeWork
    181 sg:pub.10.1038/nature08358 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025806080
    182 https://doi.org/10.1038/nature08358
    183 rdf:type schema:CreativeWork
    184 sg:pub.10.1038/nature10158 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002525491
    185 https://doi.org/10.1038/nature10158
    186 rdf:type schema:CreativeWork
    187 sg:pub.10.1038/nbt801 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042004278
    188 https://doi.org/10.1038/nbt801
    189 rdf:type schema:CreativeWork
    190 sg:pub.10.1038/nrmicro1064 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007773140
    191 https://doi.org/10.1038/nrmicro1064
    192 rdf:type schema:CreativeWork
    193 sg:pub.10.1038/nrmicro1549 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028588894
    194 https://doi.org/10.1038/nrmicro1549
    195 rdf:type schema:CreativeWork
    196 sg:pub.10.1186/1472-6750-14-50 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016051380
    197 https://doi.org/10.1186/1472-6750-14-50
    198 rdf:type schema:CreativeWork
    199 sg:pub.10.1186/1746-4811-9-37 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025042771
    200 https://doi.org/10.1186/1746-4811-9-37
    201 rdf:type schema:CreativeWork
    202 https://doi.org/10.1016/j.cropro.2015.07.018 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003053464
    203 rdf:type schema:CreativeWork
    204 https://doi.org/10.1016/j.njas.2014.05.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008128603
    205 rdf:type schema:CreativeWork
    206 https://doi.org/10.1038/sj.embor.7400769 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025213095
    207 rdf:type schema:CreativeWork
    208 https://doi.org/10.1080/09670879309371781 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008997861
    209 rdf:type schema:CreativeWork
    210 https://doi.org/10.1094/mpmi-01-12-0010-r schema:sameAs https://app.dimensions.ai/details/publication/pub.1060077348
    211 rdf:type schema:CreativeWork
    212 https://doi.org/10.1094/mpmi-11-10-0276 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060078082
    213 rdf:type schema:CreativeWork
    214 https://doi.org/10.1094/phyto-06-09-0148 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060099249
    215 rdf:type schema:CreativeWork
    216 https://doi.org/10.1094/phyto-67-415 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060105130
    217 rdf:type schema:CreativeWork
    218 https://doi.org/10.1094/phyto-78-794 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060108437
    219 rdf:type schema:CreativeWork
    220 https://doi.org/10.1094/phyto-95-0328 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060100046
    221 rdf:type schema:CreativeWork
    222 https://doi.org/10.1111/j.1365-2338.1991.tb00443.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1043759572
    223 rdf:type schema:CreativeWork
    224 https://doi.org/10.1111/j.1365-3059.2006.01491.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1053722529
    225 rdf:type schema:CreativeWork
    226 https://doi.org/10.1111/j.1365-3059.2007.01571.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1010613094
    227 rdf:type schema:CreativeWork
    228 https://doi.org/10.1111/mpp.12036 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000809331
    229 rdf:type schema:CreativeWork
    230 https://doi.org/10.1111/tpj.12307 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037179492
    231 rdf:type schema:CreativeWork
    232 https://doi.org/10.1146/annurev-phyto-072910-095326 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053350924
    233 rdf:type schema:CreativeWork
    234 https://doi.org/10.1371/journal.ppat.1002940 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051265373
    235 rdf:type schema:CreativeWork
    236 https://doi.org/10.2307/2529674 schema:sameAs https://app.dimensions.ai/details/publication/pub.1069975294
    237 rdf:type schema:CreativeWork
    238 https://www.grid.ac/institutes/grid.4818.5 schema:alternateName Wageningen University & Research
    239 schema:name Wageningen University and Research Centre, P.O. Box 16, 6700 AA, Wageningen, The Netherlands
    240 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...