Celestine blue as a new indicator in electrochemical DNA biosensors View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2016-01

AUTHORS

Ezat Hamidi-Asl, Jahan Bakhsh Raoof, Abdolraoof Samadi Meibodi, Zahra Haghnavaz Bazgir

ABSTRACT

Celestine blue (CB) was introduced as a new electroactive indicator in DNA biosensors. The interaction of CB with DNA was investigated by electrochemical and spectroscopic methods. The effect of buffer kind and pH on the electrochemical behavior of CB was studied. The peak currents of CB were linearly related to DNA concentration in the range of 5.0×10-9 to 1.0×10-7 mol/L. The detection limit of this approach was 4.76×10-10 mol/L. Based on spectrometry data a hypochromic effect was observed in UV-Vis spectra of CB with increasing DNA concentration. The results illustrate the possible interaction mode between CB and DNA is electrostatic binding. More... »

PAGES

128-134

References to SciGraph publications

Journal

TITLE

Science China Chemistry

ISSUE

1

VOLUME

59

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s11426-015-5445-3

DOI

http://dx.doi.org/10.1007/s11426-015-5445-3

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1030538574


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