Enhanced refolding of lysozyme with imidazolium-based room temperature ionic liquids: Effect of hydrophobicity and sulfur residue View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2012-08

AUTHORS

Sang-Woo Bae, Woo-Jin Chang, Yoon-Mo Koo, Sung Ho Ha

ABSTRACT

The expression of recombinant proteins in microorganism frequently leads to the formation of insoluble aggregates, inclusion bodies (IBs). Thus, the additional in vitro protein refolding process is required to convert inactive IBs into water-soluble active proteins. This study investigated the effect of sulfur residue and hydrophobicity of imidazolium-based room temperature ionic liquids (RTILs) on the refolding of lysozyme as a model protein in the batch dilution method which is the most commonly used refolding method. When lysozyme was refolded in the refolding buffer containing [BF4]-based RTILs with a systematic variety of alkyl chain on cations varying from two to eight, less hydrophobic imidazolium cations having shorter alkyl chains were effective to facilitate lysozyme refolding. Compared to the conventional refolding buffer, 2 times higher lysozyme refolding yield was obtained in 1-ethyl-3-methylimidazolium tetrafluoroborate ([EMIM][BF4]) containing refolding buffer. The refolding yield of lysozyme was even more increased by 2.5 times when 1-butyl-3-methylimidazolium methylsulfate ([BMIM][MS]) containing sulfur residue on anion was used. The sulfur residue in [BMIM][MS] is supposed to improve the refolding yield of lysozyme which has 4 intramolecular disulfide bonds. For dilution-based refolding of lysozyme, the optimum concentrations of RTILs in refolding buffer were found to be 1.0 M [EMIM][BF4] and 0.5 M [BMIM][MS], respectively. The optimum temperate for dilution-based refolding of lysozyme with RTILs was 4 °C. More... »

PAGES

1657-1662

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s11426-012-4678-7

DOI

http://dx.doi.org/10.1007/s11426-012-4678-7

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1051822357


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0306", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Physical Chemistry (incl. Structural)", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/03", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Chemical Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Inha University", 
          "id": "https://www.grid.ac/institutes/grid.202119.9", 
          "name": [
            "Department of Biological Engineering, Inha University, 402-751, Incheon, Republic of Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bae", 
        "givenName": "Sang-Woo", 
        "id": "sg:person.01170007461.17", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01170007461.17"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Wisconsin\u2013Milwaukee", 
          "id": "https://www.grid.ac/institutes/grid.267468.9", 
          "name": [
            "Department of Mechanical Engineering, School of Freshwater Sciences, University of Wisconsin-Milwaukee, 53201-0413, Milwaukee, WI, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Chang", 
        "givenName": "Woo-Jin", 
        "id": "sg:person.01233213336.41", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01233213336.41"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Inha University", 
          "id": "https://www.grid.ac/institutes/grid.202119.9", 
          "name": [
            "Department of Biological Engineering, Inha University, 402-751, Incheon, Republic of Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Koo", 
        "givenName": "Yoon-Mo", 
        "id": "sg:person.01313270030.22", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01313270030.22"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Hannam University", 
          "id": "https://www.grid.ac/institutes/grid.411970.a", 
          "name": [
            "Department of Chemical Engineering and Nano-Bio Technology, Hannam University, 305-811, Daejeon, Republic of Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ha", 
        "givenName": "Sung Ho", 
        "id": "sg:person.0633272061.13", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0633272061.13"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/s10953-009-9429-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002887196", 
          "https://doi.org/10.1007/s10953-009-9429-9"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10953-009-9429-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002887196", 
          "https://doi.org/10.1007/s10953-009-9429-9"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0958-1669(98)80109-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003279996"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jctb.1449", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004492270"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jctb.1449", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004492270"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02705783", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007618426", 
          "https://doi.org/10.1007/bf02705783"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02705783", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007618426", 
          "https://doi.org/10.1007/bf02705783"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m403207200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009687117"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/b103275p", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009699294"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s11814-011-0268-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010352195", 
          "https://doi.org/10.1007/s11814-011-0268-1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1432-1033.2003.03850.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011604306"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/(sici)1097-0290(19960405)50:1<16::aid-bit3>3.0.co;2-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013160920"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1110/ps.051596605", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015648474"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1110/ps.051596605", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015648474"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0006-3002(52)90045-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016042812"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0006-3002(52)90045-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016042812"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0958-1669(00)00200-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017377879"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s12257-009-0216-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024163312", 
          "https://doi.org/10.1007/s12257-009-0216-7"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s12257-009-0216-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024163312", 
          "https://doi.org/10.1007/s12257-009-0216-7"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/9783527619498.ch71", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027361384"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1110/ps.9.10.2001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028231275"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/cr050948h", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034047157"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/cr050948h", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034047157"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s11814-010-0386-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038327576", 
          "https://doi.org/10.1007/s11814-010-0386-1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s11814-010-0386-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038327576", 
          "https://doi.org/10.1007/s11814-010-0386-1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1080/00986440600586537", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040028985"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jbiotec.2009.04.011", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040301456"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.chroma.2010.10.028", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044151173"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.biortech.2010.07.108", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044714707"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.271.7.3478", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045607449"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.chroma.2003.09.013", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048899913"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.chroma.2003.09.013", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048899913"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0167-7799(03)00008-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050082586"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0167-7799(03)00008-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050082586"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.procbio.2010.03.030", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050315472"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi00421a014", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055175171"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja028156h", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055831602"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja028156h", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055831602"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/protein/1.2.141", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059980311"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2174/0929866054696109", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1069157660"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077094087", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2012-08", 
    "datePublishedReg": "2012-08-01", 
    "description": "The expression of recombinant proteins in microorganism frequently leads to the formation of insoluble aggregates, inclusion bodies (IBs). Thus, the additional in vitro protein refolding process is required to convert inactive IBs into water-soluble active proteins. This study investigated the effect of sulfur residue and hydrophobicity of imidazolium-based room temperature ionic liquids (RTILs) on the refolding of lysozyme as a model protein in the batch dilution method which is the most commonly used refolding method. When lysozyme was refolded in the refolding buffer containing [BF4]-based RTILs with a systematic variety of alkyl chain on cations varying from two to eight, less hydrophobic imidazolium cations having shorter alkyl chains were effective to facilitate lysozyme refolding. Compared to the conventional refolding buffer, 2 times higher lysozyme refolding yield was obtained in 1-ethyl-3-methylimidazolium tetrafluoroborate ([EMIM][BF4]) containing refolding buffer. The refolding yield of lysozyme was even more increased by 2.5 times when 1-butyl-3-methylimidazolium methylsulfate ([BMIM][MS]) containing sulfur residue on anion was used. The sulfur residue in [BMIM][MS] is supposed to improve the refolding yield of lysozyme which has 4 intramolecular disulfide bonds. For dilution-based refolding of lysozyme, the optimum concentrations of RTILs in refolding buffer were found to be 1.0 M [EMIM][BF4] and 0.5 M [BMIM][MS], respectively. The optimum temperate for dilution-based refolding of lysozyme with RTILs was 4 \u00b0C.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s11426-012-4678-7", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1297513", 
        "issn": [
          "1674-7291", 
          "1862-2771"
        ], 
        "name": "Science China Chemistry", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "8", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "55"
      }
    ], 
    "name": "Enhanced refolding of lysozyme with imidazolium-based room temperature ionic liquids: Effect of hydrophobicity and sulfur residue", 
    "pagination": "1657-1662", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "f6da7233dbe65ff5d9907f80f1023f6a146a0890e65e61870dc0d640d4e1852a"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s11426-012-4678-7"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1051822357"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s11426-012-4678-7", 
      "https://app.dimensions.ai/details/publication/pub.1051822357"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T18:23", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8675_00000524.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007%2Fs11426-012-4678-7"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s11426-012-4678-7'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s11426-012-4678-7'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s11426-012-4678-7'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s11426-012-4678-7'


 

This table displays all metadata directly associated to this object as RDF triples.

182 TRIPLES      21 PREDICATES      57 URIs      19 LITERALS      7 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s11426-012-4678-7 schema:about anzsrc-for:03
2 anzsrc-for:0306
3 schema:author N69d7a649d94641c390021fc91fcd439d
4 schema:citation sg:pub.10.1007/bf02705783
5 sg:pub.10.1007/s10953-009-9429-9
6 sg:pub.10.1007/s11814-010-0386-1
7 sg:pub.10.1007/s11814-011-0268-1
8 sg:pub.10.1007/s12257-009-0216-7
9 https://app.dimensions.ai/details/publication/pub.1077094087
10 https://doi.org/10.1002/(sici)1097-0290(19960405)50:1<16::aid-bit3>3.0.co;2-4
11 https://doi.org/10.1002/9783527619498.ch71
12 https://doi.org/10.1002/jctb.1449
13 https://doi.org/10.1016/0006-3002(52)90045-0
14 https://doi.org/10.1016/j.biortech.2010.07.108
15 https://doi.org/10.1016/j.chroma.2003.09.013
16 https://doi.org/10.1016/j.chroma.2010.10.028
17 https://doi.org/10.1016/j.jbiotec.2009.04.011
18 https://doi.org/10.1016/j.procbio.2010.03.030
19 https://doi.org/10.1016/s0167-7799(03)00008-8
20 https://doi.org/10.1016/s0958-1669(00)00200-7
21 https://doi.org/10.1016/s0958-1669(98)80109-2
22 https://doi.org/10.1021/bi00421a014
23 https://doi.org/10.1021/cr050948h
24 https://doi.org/10.1021/ja028156h
25 https://doi.org/10.1039/b103275p
26 https://doi.org/10.1046/j.1432-1033.2003.03850.x
27 https://doi.org/10.1074/jbc.271.7.3478
28 https://doi.org/10.1074/jbc.m403207200
29 https://doi.org/10.1080/00986440600586537
30 https://doi.org/10.1093/protein/1.2.141
31 https://doi.org/10.1110/ps.051596605
32 https://doi.org/10.1110/ps.9.10.2001
33 https://doi.org/10.2174/0929866054696109
34 schema:datePublished 2012-08
35 schema:datePublishedReg 2012-08-01
36 schema:description The expression of recombinant proteins in microorganism frequently leads to the formation of insoluble aggregates, inclusion bodies (IBs). Thus, the additional in vitro protein refolding process is required to convert inactive IBs into water-soluble active proteins. This study investigated the effect of sulfur residue and hydrophobicity of imidazolium-based room temperature ionic liquids (RTILs) on the refolding of lysozyme as a model protein in the batch dilution method which is the most commonly used refolding method. When lysozyme was refolded in the refolding buffer containing [BF4]-based RTILs with a systematic variety of alkyl chain on cations varying from two to eight, less hydrophobic imidazolium cations having shorter alkyl chains were effective to facilitate lysozyme refolding. Compared to the conventional refolding buffer, 2 times higher lysozyme refolding yield was obtained in 1-ethyl-3-methylimidazolium tetrafluoroborate ([EMIM][BF4]) containing refolding buffer. The refolding yield of lysozyme was even more increased by 2.5 times when 1-butyl-3-methylimidazolium methylsulfate ([BMIM][MS]) containing sulfur residue on anion was used. The sulfur residue in [BMIM][MS] is supposed to improve the refolding yield of lysozyme which has 4 intramolecular disulfide bonds. For dilution-based refolding of lysozyme, the optimum concentrations of RTILs in refolding buffer were found to be 1.0 M [EMIM][BF4] and 0.5 M [BMIM][MS], respectively. The optimum temperate for dilution-based refolding of lysozyme with RTILs was 4 °C.
37 schema:genre research_article
38 schema:inLanguage en
39 schema:isAccessibleForFree false
40 schema:isPartOf N524cc4e21a5e4d91869155c5d2a3dabb
41 Na0dd033fcea743888d8b03d9c9d85571
42 sg:journal.1297513
43 schema:name Enhanced refolding of lysozyme with imidazolium-based room temperature ionic liquids: Effect of hydrophobicity and sulfur residue
44 schema:pagination 1657-1662
45 schema:productId N0e33b605078c46aa8dc4efd0dadc0062
46 N924446a18513419a91e9138fb0d73247
47 Nc994fecd97be4ff386b32f0fcf3bb56a
48 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051822357
49 https://doi.org/10.1007/s11426-012-4678-7
50 schema:sdDatePublished 2019-04-10T18:23
51 schema:sdLicense https://scigraph.springernature.com/explorer/license/
52 schema:sdPublisher N294eceff926a49268562764505f3fbd9
53 schema:url http://link.springer.com/10.1007%2Fs11426-012-4678-7
54 sgo:license sg:explorer/license/
55 sgo:sdDataset articles
56 rdf:type schema:ScholarlyArticle
57 N0e33b605078c46aa8dc4efd0dadc0062 schema:name readcube_id
58 schema:value f6da7233dbe65ff5d9907f80f1023f6a146a0890e65e61870dc0d640d4e1852a
59 rdf:type schema:PropertyValue
60 N294eceff926a49268562764505f3fbd9 schema:name Springer Nature - SN SciGraph project
61 rdf:type schema:Organization
62 N4186e8e0118f42db9a046dc9fb8a9132 rdf:first sg:person.01313270030.22
63 rdf:rest Ne3503e5b472140d19b789639d8b6175c
64 N524cc4e21a5e4d91869155c5d2a3dabb schema:issueNumber 8
65 rdf:type schema:PublicationIssue
66 N69d7a649d94641c390021fc91fcd439d rdf:first sg:person.01170007461.17
67 rdf:rest Nb4b35c78f7c1458da13cc77d41b0d577
68 N924446a18513419a91e9138fb0d73247 schema:name doi
69 schema:value 10.1007/s11426-012-4678-7
70 rdf:type schema:PropertyValue
71 Na0dd033fcea743888d8b03d9c9d85571 schema:volumeNumber 55
72 rdf:type schema:PublicationVolume
73 Nb4b35c78f7c1458da13cc77d41b0d577 rdf:first sg:person.01233213336.41
74 rdf:rest N4186e8e0118f42db9a046dc9fb8a9132
75 Nc994fecd97be4ff386b32f0fcf3bb56a schema:name dimensions_id
76 schema:value pub.1051822357
77 rdf:type schema:PropertyValue
78 Ne3503e5b472140d19b789639d8b6175c rdf:first sg:person.0633272061.13
79 rdf:rest rdf:nil
80 anzsrc-for:03 schema:inDefinedTermSet anzsrc-for:
81 schema:name Chemical Sciences
82 rdf:type schema:DefinedTerm
83 anzsrc-for:0306 schema:inDefinedTermSet anzsrc-for:
84 schema:name Physical Chemistry (incl. Structural)
85 rdf:type schema:DefinedTerm
86 sg:journal.1297513 schema:issn 1674-7291
87 1862-2771
88 schema:name Science China Chemistry
89 rdf:type schema:Periodical
90 sg:person.01170007461.17 schema:affiliation https://www.grid.ac/institutes/grid.202119.9
91 schema:familyName Bae
92 schema:givenName Sang-Woo
93 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01170007461.17
94 rdf:type schema:Person
95 sg:person.01233213336.41 schema:affiliation https://www.grid.ac/institutes/grid.267468.9
96 schema:familyName Chang
97 schema:givenName Woo-Jin
98 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01233213336.41
99 rdf:type schema:Person
100 sg:person.01313270030.22 schema:affiliation https://www.grid.ac/institutes/grid.202119.9
101 schema:familyName Koo
102 schema:givenName Yoon-Mo
103 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01313270030.22
104 rdf:type schema:Person
105 sg:person.0633272061.13 schema:affiliation https://www.grid.ac/institutes/grid.411970.a
106 schema:familyName Ha
107 schema:givenName Sung Ho
108 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0633272061.13
109 rdf:type schema:Person
110 sg:pub.10.1007/bf02705783 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007618426
111 https://doi.org/10.1007/bf02705783
112 rdf:type schema:CreativeWork
113 sg:pub.10.1007/s10953-009-9429-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002887196
114 https://doi.org/10.1007/s10953-009-9429-9
115 rdf:type schema:CreativeWork
116 sg:pub.10.1007/s11814-010-0386-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038327576
117 https://doi.org/10.1007/s11814-010-0386-1
118 rdf:type schema:CreativeWork
119 sg:pub.10.1007/s11814-011-0268-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010352195
120 https://doi.org/10.1007/s11814-011-0268-1
121 rdf:type schema:CreativeWork
122 sg:pub.10.1007/s12257-009-0216-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024163312
123 https://doi.org/10.1007/s12257-009-0216-7
124 rdf:type schema:CreativeWork
125 https://app.dimensions.ai/details/publication/pub.1077094087 schema:CreativeWork
126 https://doi.org/10.1002/(sici)1097-0290(19960405)50:1<16::aid-bit3>3.0.co;2-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013160920
127 rdf:type schema:CreativeWork
128 https://doi.org/10.1002/9783527619498.ch71 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027361384
129 rdf:type schema:CreativeWork
130 https://doi.org/10.1002/jctb.1449 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004492270
131 rdf:type schema:CreativeWork
132 https://doi.org/10.1016/0006-3002(52)90045-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016042812
133 rdf:type schema:CreativeWork
134 https://doi.org/10.1016/j.biortech.2010.07.108 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044714707
135 rdf:type schema:CreativeWork
136 https://doi.org/10.1016/j.chroma.2003.09.013 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048899913
137 rdf:type schema:CreativeWork
138 https://doi.org/10.1016/j.chroma.2010.10.028 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044151173
139 rdf:type schema:CreativeWork
140 https://doi.org/10.1016/j.jbiotec.2009.04.011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040301456
141 rdf:type schema:CreativeWork
142 https://doi.org/10.1016/j.procbio.2010.03.030 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050315472
143 rdf:type schema:CreativeWork
144 https://doi.org/10.1016/s0167-7799(03)00008-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050082586
145 rdf:type schema:CreativeWork
146 https://doi.org/10.1016/s0958-1669(00)00200-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017377879
147 rdf:type schema:CreativeWork
148 https://doi.org/10.1016/s0958-1669(98)80109-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003279996
149 rdf:type schema:CreativeWork
150 https://doi.org/10.1021/bi00421a014 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055175171
151 rdf:type schema:CreativeWork
152 https://doi.org/10.1021/cr050948h schema:sameAs https://app.dimensions.ai/details/publication/pub.1034047157
153 rdf:type schema:CreativeWork
154 https://doi.org/10.1021/ja028156h schema:sameAs https://app.dimensions.ai/details/publication/pub.1055831602
155 rdf:type schema:CreativeWork
156 https://doi.org/10.1039/b103275p schema:sameAs https://app.dimensions.ai/details/publication/pub.1009699294
157 rdf:type schema:CreativeWork
158 https://doi.org/10.1046/j.1432-1033.2003.03850.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1011604306
159 rdf:type schema:CreativeWork
160 https://doi.org/10.1074/jbc.271.7.3478 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045607449
161 rdf:type schema:CreativeWork
162 https://doi.org/10.1074/jbc.m403207200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009687117
163 rdf:type schema:CreativeWork
164 https://doi.org/10.1080/00986440600586537 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040028985
165 rdf:type schema:CreativeWork
166 https://doi.org/10.1093/protein/1.2.141 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059980311
167 rdf:type schema:CreativeWork
168 https://doi.org/10.1110/ps.051596605 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015648474
169 rdf:type schema:CreativeWork
170 https://doi.org/10.1110/ps.9.10.2001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028231275
171 rdf:type schema:CreativeWork
172 https://doi.org/10.2174/0929866054696109 schema:sameAs https://app.dimensions.ai/details/publication/pub.1069157660
173 rdf:type schema:CreativeWork
174 https://www.grid.ac/institutes/grid.202119.9 schema:alternateName Inha University
175 schema:name Department of Biological Engineering, Inha University, 402-751, Incheon, Republic of Korea
176 rdf:type schema:Organization
177 https://www.grid.ac/institutes/grid.267468.9 schema:alternateName University of Wisconsin–Milwaukee
178 schema:name Department of Mechanical Engineering, School of Freshwater Sciences, University of Wisconsin-Milwaukee, 53201-0413, Milwaukee, WI, USA
179 rdf:type schema:Organization
180 https://www.grid.ac/institutes/grid.411970.a schema:alternateName Hannam University
181 schema:name Department of Chemical Engineering and Nano-Bio Technology, Hannam University, 305-811, Daejeon, Republic of Korea
182 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...