Death and Proliferation Time Course of Stem Cells Transplanted in the Myocardium View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2009-11

AUTHORS

Hui Qiao, Suleman Surti, Seok Rye Choi, Karthik Raju, Hualei Zhang, Datta E. Ponde, Hank F. Kung, Joel Karp, Rong Zhou

ABSTRACT

PURPOSE: Noninvasive positron emission tomography (PET) imaging of reporter gene is combined with quantitative real-time polymerase reverse transcription (RT-PCR) method to study the time course of death and proliferation of stem cells transplanted in the myocardium. METHODS: Male murine embryonic stem cells (ESCs) were stably transfected with a mutant version of herpes simplex virus type 1 thymidine kinase (HSV1-sr39tk) reporter gene; 5 x 10(6) such cells were injected into the myocardium of female athymic rats. While the transplanted cells was monitored by in vivo 9-(4-[F-18]fluoro-3-hydroxymethylbutyl)guanine ([F-18]FHBG) PET imaging of the heart, their absolute number was estimated by RT-PCR from hearts harvested at 3-5 h, 24 h, days 4, 7, and 14 after transplantation. RESULTS: (1) Forty percent of injected cells were retained in the heart while majority of injected cells were lost within a few hours after injection. Cell death was peaked at 24 h when 18% of donor cells retained in the heart were dead. (2) The substantial cell loss was reversed by significant proliferation of ESCs. This led to the recovery of cell number to 3.4 million (70% of injected dose) at day 4 and first visual observation of in vivo [F-18] signal in the heart. (3) A robust correlation (R (2) = 0.9) between percent of injected dose per gram of tissue derived from in vivo PET signal and the number of donor cells estimated by RT-PCR was revealed. CONCLUSIONS: The time course of transplanted stem cells surviving in the heart reveals a process of substantial cell loss within 24 h of injection and subsequent recovery of cell number through proliferation. Such proliferation can be noninvasively monitored by reporter gene imaging. More... »

PAGES

408

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s11307-009-0222-3

DOI

http://dx.doi.org/10.1007/s11307-009-0222-3

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1043920142

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/19459013


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1004", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical Biotechnology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/10", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Technology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Apoptosis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Death", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Proliferation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Survival", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cells, Cultured", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Culture Media, Serum-Free", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Embryonic Stem Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Female", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fluorodeoxyglucose F18", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genes, Reporter", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Herpesvirus 1, Human", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "In Situ Nick-End Labeling", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Kinetics", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Male", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mesenchymal Stem Cell Transplantation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice, Nude", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mutation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Myocardium", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Phantoms, Imaging", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Positron-Emission Tomography", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Radiopharmaceuticals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Reverse Transcriptase Polymerase Chain Reaction", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Thymidine Kinase", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transfection", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of Pennsylvania", 
          "id": "https://www.grid.ac/institutes/grid.25879.31", 
          "name": [
            "Department of Radiology, University of Pennsylvania, 19104, Philadelphia, PA, USA", 
            "Laboratories of Molecular Imaging, Department of Radiology, University of Pennsylvania, 19104, Philadelphia, PA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Qiao", 
        "givenName": "Hui", 
        "id": "sg:person.01023175524.06", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01023175524.06"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Pennsylvania", 
          "id": "https://www.grid.ac/institutes/grid.25879.31", 
          "name": [
            "Department of Radiology, University of Pennsylvania, 19104, Philadelphia, PA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Surti", 
        "givenName": "Suleman", 
        "id": "sg:person.01053503413.15", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01053503413.15"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Pennsylvania", 
          "id": "https://www.grid.ac/institutes/grid.25879.31", 
          "name": [
            "Department of Radiology, University of Pennsylvania, 19104, Philadelphia, PA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Choi", 
        "givenName": "Seok Rye", 
        "id": "sg:person.011444701337.51", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011444701337.51"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Pennsylvania", 
          "id": "https://www.grid.ac/institutes/grid.25879.31", 
          "name": [
            "Department of Radiology, University of Pennsylvania, 19104, Philadelphia, PA, USA", 
            "Laboratories of Molecular Imaging, Department of Radiology, University of Pennsylvania, 19104, Philadelphia, PA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Raju", 
        "givenName": "Karthik", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Pennsylvania", 
          "id": "https://www.grid.ac/institutes/grid.25879.31", 
          "name": [
            "Department of Bioengineering, University of Pennsylvania, 19104, Philadelphia, PA, USA", 
            "Laboratories of Molecular Imaging, Department of Radiology, University of Pennsylvania, 19104, Philadelphia, PA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zhang", 
        "givenName": "Hualei", 
        "id": "sg:person.0715447276.77", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0715447276.77"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Pennsylvania", 
          "id": "https://www.grid.ac/institutes/grid.25879.31", 
          "name": [
            "Department of Radiology, University of Pennsylvania, 19104, Philadelphia, PA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ponde", 
        "givenName": "Datta E.", 
        "id": "sg:person.0604373371.75", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0604373371.75"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Pennsylvania", 
          "id": "https://www.grid.ac/institutes/grid.25879.31", 
          "name": [
            "Department of Radiology, University of Pennsylvania, 19104, Philadelphia, PA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kung", 
        "givenName": "Hank F.", 
        "id": "sg:person.0615735606.55", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0615735606.55"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Pennsylvania", 
          "id": "https://www.grid.ac/institutes/grid.25879.31", 
          "name": [
            "Department of Radiology, University of Pennsylvania, 19104, Philadelphia, PA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Karp", 
        "givenName": "Joel", 
        "id": "sg:person.010611461772.35", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010611461772.35"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Pennsylvania", 
          "id": "https://www.grid.ac/institutes/grid.25879.31", 
          "name": [
            "Department of Radiology, University of Pennsylvania, 19104, Philadelphia, PA, USA", 
            "Laboratories of Molecular Imaging, Department of Radiology, University of Pennsylvania, 19104, Philadelphia, PA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zhou", 
        "givenName": "Rong", 
        "id": "sg:person.01120576542.42", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01120576542.42"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1161/circulationaha.105.588954", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001671975"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1634/stemcells.2007-0041", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010906796"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/jmcc.2001.1491", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012459106"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/jnen/62.9.951", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017620398"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/jmcc.2001.1367", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022231851"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0969-8051(03)00096-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022255627"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.081508598", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024531063"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1161/01.cir.0000091252.20010.6e", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032718324"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jacc.2006.08.026", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034997359"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1080/14653240701452065", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039912128"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.052709599", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050497920"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1148/radiol.2503080205", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052079858"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cardiores.2004.10.001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053484796"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1109/23.910827", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1061132678"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1109/tmi.2005.844078", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1061694701"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1109/tns.2003.817950", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1061732024"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1076887964", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1076964116", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077173976", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2009-11", 
    "datePublishedReg": "2009-11-01", 
    "description": "PURPOSE: Noninvasive positron emission tomography (PET) imaging of reporter gene is combined with quantitative real-time polymerase reverse transcription (RT-PCR) method to study the time course of death and proliferation of stem cells transplanted in the myocardium.\nMETHODS: Male murine embryonic stem cells (ESCs) were stably transfected with a mutant version of herpes simplex virus type 1 thymidine kinase (HSV1-sr39tk) reporter gene; 5 x 10(6) such cells were injected into the myocardium of female athymic rats. While the transplanted cells was monitored by in vivo 9-(4-[F-18]fluoro-3-hydroxymethylbutyl)guanine ([F-18]FHBG) PET imaging of the heart, their absolute number was estimated by RT-PCR from hearts harvested at 3-5 h, 24 h, days 4, 7, and 14 after transplantation.\nRESULTS: (1) Forty percent of injected cells were retained in the heart while majority of injected cells were lost within a few hours after injection. Cell death was peaked at 24 h when 18% of donor cells retained in the heart were dead. (2) The substantial cell loss was reversed by significant proliferation of ESCs. This led to the recovery of cell number to 3.4 million (70% of injected dose) at day 4 and first visual observation of in vivo [F-18] signal in the heart. (3) A robust correlation (R (2) = 0.9) between percent of injected dose per gram of tissue derived from in vivo PET signal and the number of donor cells estimated by RT-PCR was revealed.\nCONCLUSIONS: The time course of transplanted stem cells surviving in the heart reveals a process of substantial cell loss within 24 h of injection and subsequent recovery of cell number through proliferation. Such proliferation can be noninvasively monitored by reporter gene imaging.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s11307-009-0222-3", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.2610026", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2540550", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1118463", 
        "issn": [
          "1536-1632", 
          "1878-5751"
        ], 
        "name": "Molecular Imaging and Biology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "6", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "11"
      }
    ], 
    "name": "Death and Proliferation Time Course of Stem Cells Transplanted in the Myocardium", 
    "pagination": "408", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "d1357ce7a6a3a5ad0e17c8100247fbfbd2ead04c46d88dfa94c1d1385b174dbf"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "19459013"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "101125610"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s11307-009-0222-3"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1043920142"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s11307-009-0222-3", 
      "https://app.dimensions.ai/details/publication/pub.1043920142"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T09:18", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000339_0000000339/records_109493_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007%2Fs11307-009-0222-3"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s11307-009-0222-3'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s11307-009-0222-3'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s11307-009-0222-3'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s11307-009-0222-3'


 

This table displays all metadata directly associated to this object as RDF triples.

288 TRIPLES      21 PREDICATES      74 URIs      47 LITERALS      35 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s11307-009-0222-3 schema:about N0c8c1b5b27684a36b02f23a925327020
2 N0d7eaa6e76474740adb65ec42d574214
3 N1f79193df6094bcdafd00f0be7a87923
4 N269f84ff9b31400c807b9fe940a52b7d
5 N2c0ac81da4664c668eae81281fc5aee9
6 N3bcc5781953d4942bd25b143030237aa
7 N4de27be434aa42dc881fa4fcbf87ee4f
8 N4e9fc53ac6744b6ab1a234e63c07ac7a
9 N52074ae9ae2d4f5c9bcd3c9150177071
10 N52c7ee628164411487764a92b2061058
11 N6cc8c92aa2524de3a1b95acf323f03c7
12 N6e7a0081bbce4cab8acdbdb2e6954733
13 N7d28054e389d4957ba7b98f743a5384a
14 N8934094f7714483bad4ca7e8db8d4111
15 N896ab63e35bb488d96600b7bb1efad6e
16 Na5453448d09d4e44b233712d869b3ec9
17 Nb1474ff51463426d972cfe07f1dc068a
18 Nb3a54379804949909edf31fc3f055be2
19 Nc0843d56b36f49ed9ec7952cfc1fb021
20 Ncbcf58667b024450a0c76ec72232969a
21 Ne0b81d53545c44fbbb70f3b960ec114b
22 Ne17b786a3e7f4c4bb9f3aff58aa187a4
23 Ne4aadd52a4364809915f1089e5f34d34
24 Ne8b796831226430ba135cfe788db886c
25 Nedfc116c28a6474698688fe278d03bb1
26 Nff4c74226ce54a6aa3aa08829887ea8b
27 anzsrc-for:10
28 anzsrc-for:1004
29 schema:author Nc07e49e44dfb4044a92417526241d335
30 schema:citation https://app.dimensions.ai/details/publication/pub.1076887964
31 https://app.dimensions.ai/details/publication/pub.1076964116
32 https://app.dimensions.ai/details/publication/pub.1077173976
33 https://doi.org/10.1006/jmcc.2001.1367
34 https://doi.org/10.1006/jmcc.2001.1491
35 https://doi.org/10.1016/j.cardiores.2004.10.001
36 https://doi.org/10.1016/j.jacc.2006.08.026
37 https://doi.org/10.1016/s0969-8051(03)00096-9
38 https://doi.org/10.1073/pnas.052709599
39 https://doi.org/10.1073/pnas.081508598
40 https://doi.org/10.1080/14653240701452065
41 https://doi.org/10.1093/jnen/62.9.951
42 https://doi.org/10.1109/23.910827
43 https://doi.org/10.1109/tmi.2005.844078
44 https://doi.org/10.1109/tns.2003.817950
45 https://doi.org/10.1148/radiol.2503080205
46 https://doi.org/10.1161/01.cir.0000091252.20010.6e
47 https://doi.org/10.1161/circulationaha.105.588954
48 https://doi.org/10.1634/stemcells.2007-0041
49 schema:datePublished 2009-11
50 schema:datePublishedReg 2009-11-01
51 schema:description PURPOSE: Noninvasive positron emission tomography (PET) imaging of reporter gene is combined with quantitative real-time polymerase reverse transcription (RT-PCR) method to study the time course of death and proliferation of stem cells transplanted in the myocardium. METHODS: Male murine embryonic stem cells (ESCs) were stably transfected with a mutant version of herpes simplex virus type 1 thymidine kinase (HSV1-sr39tk) reporter gene; 5 x 10(6) such cells were injected into the myocardium of female athymic rats. While the transplanted cells was monitored by in vivo 9-(4-[F-18]fluoro-3-hydroxymethylbutyl)guanine ([F-18]FHBG) PET imaging of the heart, their absolute number was estimated by RT-PCR from hearts harvested at 3-5 h, 24 h, days 4, 7, and 14 after transplantation. RESULTS: (1) Forty percent of injected cells were retained in the heart while majority of injected cells were lost within a few hours after injection. Cell death was peaked at 24 h when 18% of donor cells retained in the heart were dead. (2) The substantial cell loss was reversed by significant proliferation of ESCs. This led to the recovery of cell number to 3.4 million (70% of injected dose) at day 4 and first visual observation of in vivo [F-18] signal in the heart. (3) A robust correlation (R (2) = 0.9) between percent of injected dose per gram of tissue derived from in vivo PET signal and the number of donor cells estimated by RT-PCR was revealed. CONCLUSIONS: The time course of transplanted stem cells surviving in the heart reveals a process of substantial cell loss within 24 h of injection and subsequent recovery of cell number through proliferation. Such proliferation can be noninvasively monitored by reporter gene imaging.
52 schema:genre research_article
53 schema:inLanguage en
54 schema:isAccessibleForFree true
55 schema:isPartOf N0296a51543a44f94b59c3cefaa2f7a3a
56 N4749e865ab9342a98ec57323f315eac9
57 sg:journal.1118463
58 schema:name Death and Proliferation Time Course of Stem Cells Transplanted in the Myocardium
59 schema:pagination 408
60 schema:productId N2359bea3e3a442be85339df86cac6f96
61 N3eb058c72a3a4b5d8ef1bed9569f8db3
62 N98728a60226e4d51882e8715f54ee740
63 N98ddc76f6a264d4da7cf7c94eb49976f
64 Ndbd8d90680624bdb8c798ecca5aed098
65 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043920142
66 https://doi.org/10.1007/s11307-009-0222-3
67 schema:sdDatePublished 2019-04-11T09:18
68 schema:sdLicense https://scigraph.springernature.com/explorer/license/
69 schema:sdPublisher N6290555ab30b421b8ba457023c0b1ebc
70 schema:url http://link.springer.com/10.1007%2Fs11307-009-0222-3
71 sgo:license sg:explorer/license/
72 sgo:sdDataset articles
73 rdf:type schema:ScholarlyArticle
74 N0296a51543a44f94b59c3cefaa2f7a3a schema:volumeNumber 11
75 rdf:type schema:PublicationVolume
76 N0c8c1b5b27684a36b02f23a925327020 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
77 schema:name Cell Survival
78 rdf:type schema:DefinedTerm
79 N0d7eaa6e76474740adb65ec42d574214 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
80 schema:name Animals
81 rdf:type schema:DefinedTerm
82 N11d06716a60648ccb3d68b71f961bceb rdf:first N363c71cb26064eb283574d939e8c8861
83 rdf:rest N78cfbeeb1bfe44508296a5cb9df7efac
84 N1894fd7e237e4f4baa56f6ee2221e70a rdf:first sg:person.0604373371.75
85 rdf:rest N96ba8fbe1338406c82b3206bab54c934
86 N1f79193df6094bcdafd00f0be7a87923 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
87 schema:name Mutation
88 rdf:type schema:DefinedTerm
89 N2359bea3e3a442be85339df86cac6f96 schema:name nlm_unique_id
90 schema:value 101125610
91 rdf:type schema:PropertyValue
92 N269f84ff9b31400c807b9fe940a52b7d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
93 schema:name Genes, Reporter
94 rdf:type schema:DefinedTerm
95 N2c0ac81da4664c668eae81281fc5aee9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
96 schema:name Culture Media, Serum-Free
97 rdf:type schema:DefinedTerm
98 N363c71cb26064eb283574d939e8c8861 schema:affiliation https://www.grid.ac/institutes/grid.25879.31
99 schema:familyName Raju
100 schema:givenName Karthik
101 rdf:type schema:Person
102 N3bcc5781953d4942bd25b143030237aa schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
103 schema:name Myocardium
104 rdf:type schema:DefinedTerm
105 N3eb058c72a3a4b5d8ef1bed9569f8db3 schema:name readcube_id
106 schema:value d1357ce7a6a3a5ad0e17c8100247fbfbd2ead04c46d88dfa94c1d1385b174dbf
107 rdf:type schema:PropertyValue
108 N4749e865ab9342a98ec57323f315eac9 schema:issueNumber 6
109 rdf:type schema:PublicationIssue
110 N4de27be434aa42dc881fa4fcbf87ee4f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
111 schema:name Mice
112 rdf:type schema:DefinedTerm
113 N4e9fc53ac6744b6ab1a234e63c07ac7a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
114 schema:name Positron-Emission Tomography
115 rdf:type schema:DefinedTerm
116 N52074ae9ae2d4f5c9bcd3c9150177071 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
117 schema:name Female
118 rdf:type schema:DefinedTerm
119 N52c7ee628164411487764a92b2061058 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
120 schema:name Radiopharmaceuticals
121 rdf:type schema:DefinedTerm
122 N6290555ab30b421b8ba457023c0b1ebc schema:name Springer Nature - SN SciGraph project
123 rdf:type schema:Organization
124 N6cc8c92aa2524de3a1b95acf323f03c7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
125 schema:name Herpesvirus 1, Human
126 rdf:type schema:DefinedTerm
127 N6e7a0081bbce4cab8acdbdb2e6954733 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
128 schema:name Apoptosis
129 rdf:type schema:DefinedTerm
130 N71cbf5ade29648598c7d3b82accdfe2f rdf:first sg:person.01053503413.15
131 rdf:rest Ncaeaea293b29457abfdd5d10de257331
132 N78cfbeeb1bfe44508296a5cb9df7efac rdf:first sg:person.0715447276.77
133 rdf:rest N1894fd7e237e4f4baa56f6ee2221e70a
134 N799854c6c2774336b3711c97032e021b rdf:first sg:person.010611461772.35
135 rdf:rest Nf8b3856513474c108cd159d06b696a52
136 N7d28054e389d4957ba7b98f743a5384a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
137 schema:name Cells, Cultured
138 rdf:type schema:DefinedTerm
139 N8934094f7714483bad4ca7e8db8d4111 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
140 schema:name Cell Death
141 rdf:type schema:DefinedTerm
142 N896ab63e35bb488d96600b7bb1efad6e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
143 schema:name Reverse Transcriptase Polymerase Chain Reaction
144 rdf:type schema:DefinedTerm
145 N96ba8fbe1338406c82b3206bab54c934 rdf:first sg:person.0615735606.55
146 rdf:rest N799854c6c2774336b3711c97032e021b
147 N98728a60226e4d51882e8715f54ee740 schema:name doi
148 schema:value 10.1007/s11307-009-0222-3
149 rdf:type schema:PropertyValue
150 N98ddc76f6a264d4da7cf7c94eb49976f schema:name dimensions_id
151 schema:value pub.1043920142
152 rdf:type schema:PropertyValue
153 Na5453448d09d4e44b233712d869b3ec9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
154 schema:name Kinetics
155 rdf:type schema:DefinedTerm
156 Nb1474ff51463426d972cfe07f1dc068a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
157 schema:name Phantoms, Imaging
158 rdf:type schema:DefinedTerm
159 Nb3a54379804949909edf31fc3f055be2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
160 schema:name Transfection
161 rdf:type schema:DefinedTerm
162 Nc07e49e44dfb4044a92417526241d335 rdf:first sg:person.01023175524.06
163 rdf:rest N71cbf5ade29648598c7d3b82accdfe2f
164 Nc0843d56b36f49ed9ec7952cfc1fb021 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
165 schema:name Mesenchymal Stem Cell Transplantation
166 rdf:type schema:DefinedTerm
167 Ncaeaea293b29457abfdd5d10de257331 rdf:first sg:person.011444701337.51
168 rdf:rest N11d06716a60648ccb3d68b71f961bceb
169 Ncbcf58667b024450a0c76ec72232969a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
170 schema:name Thymidine Kinase
171 rdf:type schema:DefinedTerm
172 Ndbd8d90680624bdb8c798ecca5aed098 schema:name pubmed_id
173 schema:value 19459013
174 rdf:type schema:PropertyValue
175 Ne0b81d53545c44fbbb70f3b960ec114b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
176 schema:name Male
177 rdf:type schema:DefinedTerm
178 Ne17b786a3e7f4c4bb9f3aff58aa187a4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
179 schema:name Mice, Nude
180 rdf:type schema:DefinedTerm
181 Ne4aadd52a4364809915f1089e5f34d34 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
182 schema:name Cell Proliferation
183 rdf:type schema:DefinedTerm
184 Ne8b796831226430ba135cfe788db886c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
185 schema:name Embryonic Stem Cells
186 rdf:type schema:DefinedTerm
187 Nedfc116c28a6474698688fe278d03bb1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
188 schema:name In Situ Nick-End Labeling
189 rdf:type schema:DefinedTerm
190 Nf8b3856513474c108cd159d06b696a52 rdf:first sg:person.01120576542.42
191 rdf:rest rdf:nil
192 Nff4c74226ce54a6aa3aa08829887ea8b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
193 schema:name Fluorodeoxyglucose F18
194 rdf:type schema:DefinedTerm
195 anzsrc-for:10 schema:inDefinedTermSet anzsrc-for:
196 schema:name Technology
197 rdf:type schema:DefinedTerm
198 anzsrc-for:1004 schema:inDefinedTermSet anzsrc-for:
199 schema:name Medical Biotechnology
200 rdf:type schema:DefinedTerm
201 sg:grant.2540550 http://pending.schema.org/fundedItem sg:pub.10.1007/s11307-009-0222-3
202 rdf:type schema:MonetaryGrant
203 sg:grant.2610026 http://pending.schema.org/fundedItem sg:pub.10.1007/s11307-009-0222-3
204 rdf:type schema:MonetaryGrant
205 sg:journal.1118463 schema:issn 1536-1632
206 1878-5751
207 schema:name Molecular Imaging and Biology
208 rdf:type schema:Periodical
209 sg:person.01023175524.06 schema:affiliation https://www.grid.ac/institutes/grid.25879.31
210 schema:familyName Qiao
211 schema:givenName Hui
212 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01023175524.06
213 rdf:type schema:Person
214 sg:person.01053503413.15 schema:affiliation https://www.grid.ac/institutes/grid.25879.31
215 schema:familyName Surti
216 schema:givenName Suleman
217 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01053503413.15
218 rdf:type schema:Person
219 sg:person.010611461772.35 schema:affiliation https://www.grid.ac/institutes/grid.25879.31
220 schema:familyName Karp
221 schema:givenName Joel
222 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010611461772.35
223 rdf:type schema:Person
224 sg:person.01120576542.42 schema:affiliation https://www.grid.ac/institutes/grid.25879.31
225 schema:familyName Zhou
226 schema:givenName Rong
227 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01120576542.42
228 rdf:type schema:Person
229 sg:person.011444701337.51 schema:affiliation https://www.grid.ac/institutes/grid.25879.31
230 schema:familyName Choi
231 schema:givenName Seok Rye
232 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011444701337.51
233 rdf:type schema:Person
234 sg:person.0604373371.75 schema:affiliation https://www.grid.ac/institutes/grid.25879.31
235 schema:familyName Ponde
236 schema:givenName Datta E.
237 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0604373371.75
238 rdf:type schema:Person
239 sg:person.0615735606.55 schema:affiliation https://www.grid.ac/institutes/grid.25879.31
240 schema:familyName Kung
241 schema:givenName Hank F.
242 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0615735606.55
243 rdf:type schema:Person
244 sg:person.0715447276.77 schema:affiliation https://www.grid.ac/institutes/grid.25879.31
245 schema:familyName Zhang
246 schema:givenName Hualei
247 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0715447276.77
248 rdf:type schema:Person
249 https://app.dimensions.ai/details/publication/pub.1076887964 schema:CreativeWork
250 https://app.dimensions.ai/details/publication/pub.1076964116 schema:CreativeWork
251 https://app.dimensions.ai/details/publication/pub.1077173976 schema:CreativeWork
252 https://doi.org/10.1006/jmcc.2001.1367 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022231851
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1006/jmcc.2001.1491 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012459106
255 rdf:type schema:CreativeWork
256 https://doi.org/10.1016/j.cardiores.2004.10.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053484796
257 rdf:type schema:CreativeWork
258 https://doi.org/10.1016/j.jacc.2006.08.026 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034997359
259 rdf:type schema:CreativeWork
260 https://doi.org/10.1016/s0969-8051(03)00096-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022255627
261 rdf:type schema:CreativeWork
262 https://doi.org/10.1073/pnas.052709599 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050497920
263 rdf:type schema:CreativeWork
264 https://doi.org/10.1073/pnas.081508598 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024531063
265 rdf:type schema:CreativeWork
266 https://doi.org/10.1080/14653240701452065 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039912128
267 rdf:type schema:CreativeWork
268 https://doi.org/10.1093/jnen/62.9.951 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017620398
269 rdf:type schema:CreativeWork
270 https://doi.org/10.1109/23.910827 schema:sameAs https://app.dimensions.ai/details/publication/pub.1061132678
271 rdf:type schema:CreativeWork
272 https://doi.org/10.1109/tmi.2005.844078 schema:sameAs https://app.dimensions.ai/details/publication/pub.1061694701
273 rdf:type schema:CreativeWork
274 https://doi.org/10.1109/tns.2003.817950 schema:sameAs https://app.dimensions.ai/details/publication/pub.1061732024
275 rdf:type schema:CreativeWork
276 https://doi.org/10.1148/radiol.2503080205 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052079858
277 rdf:type schema:CreativeWork
278 https://doi.org/10.1161/01.cir.0000091252.20010.6e schema:sameAs https://app.dimensions.ai/details/publication/pub.1032718324
279 rdf:type schema:CreativeWork
280 https://doi.org/10.1161/circulationaha.105.588954 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001671975
281 rdf:type schema:CreativeWork
282 https://doi.org/10.1634/stemcells.2007-0041 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010906796
283 rdf:type schema:CreativeWork
284 https://www.grid.ac/institutes/grid.25879.31 schema:alternateName University of Pennsylvania
285 schema:name Department of Bioengineering, University of Pennsylvania, 19104, Philadelphia, PA, USA
286 Department of Radiology, University of Pennsylvania, 19104, Philadelphia, PA, USA
287 Laboratories of Molecular Imaging, Department of Radiology, University of Pennsylvania, 19104, Philadelphia, PA, USA
288 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...