Direct Site-Specific Radiolabeling of an Affibody Protein with 4-[18F]Fluorobenzaldehyde via Oxime Chemistry View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2008-07

AUTHORS

Mohammad Namavari, Omayra Padilla De Jesus, Zhen Cheng, Abhijit De, Ernest Kovacs, Jelena Levi, Rong Zhang, Joshua K. Hoerner, Hans Grade, Faisal A. Syud, Sanjiv S. Gambhir

ABSTRACT

PURPOSE: In this study, we introduce a methodology for preparing 18F-labeled Affibody protein, specifically 18F-Anti-HER2 dimeric Affibody (14 kDa), for in vivo imaging of HER2neu with positron emission tomography (PET). PROCEDURES: We have used 4-[18F]fluorobenzaldehyde as a synthon to prepare 18F-Anti-HER2 Affibody. Aminooxy-functionalized Affibody (Anti-HER2-ONH2) was incubated with 4-[18F]fluorobenzaldehyde in ammonium acetate buffer at pH 4 in the presence of methanol at 70 degrees C for 15 min. The resulting 18F-labeled Affibody molecule was evaluated as a PET probe in xenograft models expressing HER2. RESULTS: We have successfully prepared 18F-Anti-HER2 dimeric Affibody (14 kDa), N-(4-[18F]fluorobenzylidine)oxime-Anti-HER2 Affibody, [18F]FBO-Anti-HER2, in 26-30% radiochemical yields (decay corrected). High-contrast small-animal PET images with relatively moderate tumor uptake (1.79 +/- 0.40% ID/g) were observed for the 18F-Anti-HER2 Affibody. CONCLUSION: Site-specific 18F-labeled Affibody against HER2 has been synthesized via chemoselective oxime formation between an aminooxy-functionalized Affibody and 18F-fluorobenzaldehyde. The results have implications for radiolabeling of other affibodies and macromolecules and should also be important for advancing Affibody imaging with PET. More... »

PAGES

177-181

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s11307-008-0142-7

DOI

http://dx.doi.org/10.1007/s11307-008-0142-7

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1018859516

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/18481153


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RDF/XML is a standard XML format for linked data.

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