Ontology type: schema:ScholarlyArticle Open Access: True
2016-07
AUTHORSM. G. L. Henquet, M. Roelse, R. C. H. de Vos, A. Schipper, G. Polder, N. C. A. de Ruijter, R. D. Hall, M. A. Jongsma
ABSTRACTINTRODUCTION: Metabolomics has become a valuable tool in many research areas. However, generating metabolomics-based biochemical profiles without any related bioactivity is only of indirect value in understanding a biological process. Therefore, metabolomics research could greatly benefit from tools that directly determine the bioactivity of the detected compounds. OBJECTIVE: We aimed to combine LC-MS metabolomics with a cell based receptor assay. This combination could increase the understanding of biological processes and may provide novel opportunities for functional metabolomics. METHODS: We developed a flow through biosensor with human cells expressing both the TRPV1, a calcium ion channel which responds to capsaicin, and the fluorescent intracellular calcium ion reporter, YC3.6. We have analysed three contrasting Capsicum varieties. Two were selected with contrasting degrees of spiciness for characterization by HPLC coupled to high mass resolution MS. Subsequently, the biosensor was then used to link individual pepper compounds with TRPV1 activity. RESULTS: Among the compounds in the crude pepper fruit extracts, we confirmed capsaicin and also identified both nordihydrocapsaicin and dihydrocapsaicin as true agonists of the TRPV1 receptor. Furthermore, the biosensor was able to detect receptor activity in extracts of both Capsicum fruits as well as a commercial product. Sensitivity of the biosensor to this commercial product was similar to the sensory threshold of a human sensory panel. CONCLUSION: Our results demonstrate that the TRPV1 biosensor is suitable for detecting bioactive metabolites. Novel opportunities may lie in the development of a continuous functional assay, where the biosensor is directly coupled to the LC-MS. More... »
PAGES115
http://scigraph.springernature.com/pub.10.1007/s11306-016-1057-y
DOIhttp://dx.doi.org/10.1007/s11306-016-1057-y
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1033902414
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/27398080
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biochemistry and Cell Biology",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"name": [
"BU Bioscience, WageningenUR, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands"
],
"type": "Organization"
},
"familyName": "Henquet",
"givenName": "M. G. L.",
"id": "sg:person.01173634073.49",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01173634073.49"
],
"type": "Person"
},
{
"affiliation": {
"name": [
"BU Bioscience, WageningenUR, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands",
"Laboratory of Plant Physiology, WageningenUR, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands"
],
"type": "Organization"
},
"familyName": "Roelse",
"givenName": "M.",
"id": "sg:person.01255077702.06",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01255077702.06"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Netherlands Metabolomics Centre",
"id": "https://www.grid.ac/institutes/grid.450196.f",
"name": [
"BU Bioscience, WageningenUR, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands",
"Netherlands Metabolomics Centre, Einsteinweg 55, 3335 CC, Leiden, The Netherlands"
],
"type": "Organization"
},
"familyName": "de Vos",
"givenName": "R. C. H.",
"id": "sg:person.011723350762.00",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011723350762.00"
],
"type": "Person"
},
{
"affiliation": {
"name": [
"BU Bioscience, WageningenUR, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands"
],
"type": "Organization"
},
"familyName": "Schipper",
"givenName": "A.",
"type": "Person"
},
{
"affiliation": {
"name": [
"BU Bioscience, WageningenUR, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands"
],
"type": "Organization"
},
"familyName": "Polder",
"givenName": "G.",
"id": "sg:person.016025633407.30",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016025633407.30"
],
"type": "Person"
},
{
"affiliation": {
"name": [
"Laboratory of Cell Biology, WageningenUR, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands"
],
"type": "Organization"
},
"familyName": "de Ruijter",
"givenName": "N. C. A.",
"id": "sg:person.01305065657.83",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01305065657.83"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Netherlands Metabolomics Centre",
"id": "https://www.grid.ac/institutes/grid.450196.f",
"name": [
"BU Bioscience, WageningenUR, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands",
"Netherlands Metabolomics Centre, Einsteinweg 55, 3335 CC, Leiden, The Netherlands",
"Laboratory of Plant Physiology, WageningenUR, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands"
],
"type": "Organization"
},
"familyName": "Hall",
"givenName": "R. D.",
"id": "sg:person.0704576323.20",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0704576323.20"
],
"type": "Person"
},
{
"affiliation": {
"name": [
"BU Bioscience, WageningenUR, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands"
],
"type": "Organization"
},
"familyName": "Jongsma",
"givenName": "M. A.",
"id": "sg:person.01043142737.02",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01043142737.02"
],
"type": "Person"
}
],
"citation": [
{
"id": "https://doi.org/10.1097/01.wnr.0000073429.02536.1d",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1000050649"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1097/01.wnr.0000073429.02536.1d",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1000050649"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1097/01.wnr.0000073429.02536.1d",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1000050649"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s10529-008-9882-3",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1000485579",
"https://doi.org/10.1007/s10529-008-9882-3"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s10529-008-9882-3",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1000485579",
"https://doi.org/10.1007/s10529-008-9882-3"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.cell.2008.08.040",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1002139734"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nn.2102",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1007078523",
"https://doi.org/10.1038/nn.2102"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.bios.2013.03.065",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1012742748"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/biof.5520230404",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1013991714"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0006-3495(03)70022-8",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1014203491"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.2174/138920211797248619",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1014687158"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.tibtech.2005.12.006",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1014926454"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s00216-004-2795-7",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1022692264",
"https://doi.org/10.1007/s00216-004-2795-7"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s00216-004-2795-7",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1022692264",
"https://doi.org/10.1007/s00216-004-2795-7"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.chroma.2008.09.061",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1023035036"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s11306-007-0082-2",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1023480149",
"https://doi.org/10.1007/s11306-007-0082-2"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1155/2013/792527",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1026872744"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.neuroscience.2007.07.039",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1028667169"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.chroma.2012.03.026",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1029525241"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s11306-011-0349-5",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1034174370",
"https://doi.org/10.1007/s11306-011-0349-5"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s11306-012-0432-6",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1037075460",
"https://doi.org/10.1007/s11306-012-0432-6"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.phytochem.2011.03.016",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1038848433"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s11306-014-0656-8",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1045239064",
"https://doi.org/10.1007/s11306-014-0656-8"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1073/pnas.0400417101",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1051047871"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1073/pnas.0230374100",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1053116899"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1021/ac3017339",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1055003170"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1021/ac401068j",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1055004354"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1021/np200673b",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1056231766"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1021/np200673b",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1056231766"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1021/np9007359",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1056238085"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1021/np9007359",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1056238085"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1055/s-0033-1360109",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1057301114"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.2174/138920111793937826",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1069177761"
],
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1076833368",
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1523/jneurosci.19-02-00529.1999",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1083371088"
],
"type": "CreativeWork"
}
],
"datePublished": "2016-07",
"datePublishedReg": "2016-07-01",
"description": "INTRODUCTION: Metabolomics has become a valuable tool in many research areas. However, generating metabolomics-based biochemical profiles without any related bioactivity is only of indirect value in understanding a biological process. Therefore, metabolomics research could greatly benefit from tools that directly determine the bioactivity of the detected compounds.\nOBJECTIVE: We aimed to combine LC-MS metabolomics with a cell based receptor assay. This combination could increase the understanding of biological processes and may provide novel opportunities for functional metabolomics.\nMETHODS: We developed a flow through biosensor with human cells expressing both the TRPV1, a calcium ion channel which responds to capsaicin, and the fluorescent intracellular calcium ion reporter, YC3.6. We have analysed three contrasting Capsicum varieties. Two were selected with contrasting degrees of spiciness for characterization by HPLC coupled to high mass resolution MS. Subsequently, the biosensor was then used to link individual pepper compounds with TRPV1 activity.\nRESULTS: Among the compounds in the crude pepper fruit extracts, we confirmed capsaicin and also identified both nordihydrocapsaicin and dihydrocapsaicin as true agonists of the TRPV1 receptor. Furthermore, the biosensor was able to detect receptor activity in extracts of both Capsicum fruits as well as a commercial product. Sensitivity of the biosensor to this commercial product was similar to the sensory threshold of a human sensory panel.\nCONCLUSION: Our results demonstrate that the TRPV1 biosensor is suitable for detecting bioactive metabolites. Novel opportunities may lie in the development of a continuous functional assay, where the biosensor is directly coupled to the LC-MS.",
"genre": "research_article",
"id": "sg:pub.10.1007/s11306-016-1057-y",
"inLanguage": [
"en"
],
"isAccessibleForFree": true,
"isPartOf": [
{
"id": "sg:journal.1036887",
"issn": [
"1573-3882",
"1573-3890"
],
"name": "Metabolomics",
"type": "Periodical"
},
{
"issueNumber": "7",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "12"
}
],
"name": "Metabolomics meets functional assays: coupling LC\u2013MS and microfluidic cell-based receptor-ligand analyses",
"pagination": "115",
"productId": [
{
"name": "readcube_id",
"type": "PropertyValue",
"value": [
"a1809cbdfc55369eb9fc4e947e894adf29bd595cbcbf576137ef38d6a64ff3bf"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"27398080"
]
},
{
"name": "nlm_unique_id",
"type": "PropertyValue",
"value": [
"101274889"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1007/s11306-016-1057-y"
]
},
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1033902414"
]
}
],
"sameAs": [
"https://doi.org/10.1007/s11306-016-1057-y",
"https://app.dimensions.ai/details/publication/pub.1033902414"
],
"sdDataset": "articles",
"sdDatePublished": "2019-04-11T12:38",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000363_0000000363/records_70040_00000001.jsonl",
"type": "ScholarlyArticle",
"url": "https://link.springer.com/10.1007%2Fs11306-016-1057-y"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s11306-016-1057-y'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s11306-016-1057-y'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s11306-016-1057-y'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s11306-016-1057-y'
This table displays all metadata directly associated to this object as RDF triples.
225 TRIPLES
21 PREDICATES
58 URIs
21 LITERALS
9 BLANK NODES