Metabolic profile of Mycobacterium smegmatis reveals Mce4 proteins are relevant for cell wall lipid homeostasis View Full Text


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Article Info

DATE

2016-06

AUTHORS

María Paz Santangelo, Adam Heuberger, Federico Blanco, Marina Forrellad, Catalina Taibo, Laura Klepp, Julia Sabio García, Pablo I. Nikel, Mary Jackson, Fabiana Bigi

ABSTRACT

The Mce proteins are encoded in a variable number of operons (from one to eight) in all Mycobacterium species. A role in the transport of host and mycobacterial lipids has been demonstrated for some Mce proteins in the pathogen Mycobacterium tuberculosis but little is known about these proteins in Mycobacterium smegmatis, a soil dweller species. To investigate the role of Mce proteins in M. smegmatis. We used a non-targeted GC–MS approach to define the metabolic profiles of knockout mutants in mce operons of M. smegmatis. Metabolomic analysis was complemented with thin layer chromatography and transcriptional studies. We demonstrated that the lack of Mce4 proteins alters the primary carbon metabolism of M. smegmatis by reducing the content of fatty acids and increasing the accumulation of two stress-related compounds, glutamate/glutamine and triacyl glycerol (TAG). We also provide evidence supporting that the accumulation of TAG in a Δmce4 mutant depends on the bacterial redox state. These results, together with the finding that the cell surface of the Δmce4 mutant is significantly altered, support a role for Mce4 in maintaining the cell wall lipids architecture of M. smegmatis, which, when altered, induces a redox imbalance that results in the accumulation of the stress-related compounds. More... »

PAGES

97

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s11306-016-1035-4

DOI

http://dx.doi.org/10.1007/s11306-016-1035-4

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https://app.dimensions.ai/details/publication/pub.1030240956


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40 schema:description The Mce proteins are encoded in a variable number of operons (from one to eight) in all Mycobacterium species. A role in the transport of host and mycobacterial lipids has been demonstrated for some Mce proteins in the pathogen Mycobacterium tuberculosis but little is known about these proteins in Mycobacterium smegmatis, a soil dweller species. To investigate the role of Mce proteins in M. smegmatis. We used a non-targeted GC–MS approach to define the metabolic profiles of knockout mutants in mce operons of M. smegmatis. Metabolomic analysis was complemented with thin layer chromatography and transcriptional studies. We demonstrated that the lack of Mce4 proteins alters the primary carbon metabolism of M. smegmatis by reducing the content of fatty acids and increasing the accumulation of two stress-related compounds, glutamate/glutamine and triacyl glycerol (TAG). We also provide evidence supporting that the accumulation of TAG in a Δmce4 mutant depends on the bacterial redox state. These results, together with the finding that the cell surface of the Δmce4 mutant is significantly altered, support a role for Mce4 in maintaining the cell wall lipids architecture of M. smegmatis, which, when altered, induces a redox imbalance that results in the accumulation of the stress-related compounds.
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