Development of tools for quantitative intracellular metabolomics of Aspergillus niger chemostat cultures View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2015-10

AUTHORS

Francisca Lameiras, Joseph J. Heijnen, Walter M. van Gulik

ABSTRACT

In view of the high citric acid production capacity of Aspergillus niger, it should be well suited as a cell factory for the production of other relevant acids as succinic, fumaric, itaconic and malic. Quantitative metabolomics is an important omics tool in a synthetic biology approach to develop A. niger for the production of these acids. Such studies require well defined and tightly controlled cultivation conditions and proper rapid sampling, sample processing and analysis methods. In this study we present the development of a chemostat for homogeneous steady state cultivation of A. niger, equipped with a new dedicated rapid sampling device. A quenching method for quantitative metabolomics in A. niger based on cold methanol was evaluated using balances and optimized with the aim of avoiding metabolite leakage during sample processing. The optimization was based on measurements of the intermediates of the glycolysis, TCA and PPP pathways and amino acids, using a balance approach. Leakage was found to be absent at -20 °C for a 40 % (v/v) methanol concentration in water. Under these conditions the average metabolite recovery was close to 100 %. When comparing A. niger and Penicillium chrysogenum metabolomes, under the same cultivation conditions, similar metabolite fingerprints were found in both fungi, except for the intracellular citrate level which is higher for A. niger. More... »

PAGES

1253-1264

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s11306-015-0781-z

DOI

http://dx.doi.org/10.1007/s11306-015-0781-z

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1050967534

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/26366135


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