Metabolomics reveals organ-specific metabolic rearrangements during early tomato seedling development View Full Text


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Article Info

DATE

2014-02-01

AUTHORS

Maria Victoria Gomez Roldan, Bas Engel, Ric C. H. de Vos, Pieter Vereijken, Laura Astola, Marian Groenenboom, Henri van de Geest, Arnaud Bovy, Jaap Molenaar, Fred van Eeuwijk, Robert D. Hall

ABSTRACT

Tomato seedlings (Solanum lycopersicum cv. MoneyMaker), grown under strictly controlled conditions, have been used to study alterations occurring in secondary metabolite biosynthetic pathways following developmental and environmental perturbations. Robustness and reproducibility of the system were confirmed using detailed statistical analyses of the metabolome. LCMS profiling was applied using whole germinated seeds as well as cotyledons, hypocotyls and roots from 3 to 9 days old seedlings to generate relative levels of 433 metabolites, of which 62 were annotated. Initial focus was given to the polyphenol pathway and several additional mass signals have been putatively annotated using high mass resolution fragmentation. Clear organ and developmental stage—specific differences were observed. Seeds accumulated saponin-like compounds; roots accumulated mainly alkaloids; cotyledons contained mainly glycosylated flavonols and; hypocotyls contained mainly anthocyanins. For each organ, the developmental changes in metabolite profiles were described by using linear mixed models. Across three independent experiments, 85 % of the metabolites showed similar developmental trends. This tomato seedling system has given us valuable additional insights into the complexity of seedling secondary metabolism. How metabolic profiles reflect an interplay between depletion of stored molecules and de novo synthesis and how the overall picture for this important crop plant contrasts to e.g. Arabidopsis are emphasised. More... »

PAGES

958-974

References to SciGraph publications

  • 2008-10-26. Enrichment of tomato fruit with health-promoting anthocyanins by expression of select transcription factors in NATURE BIOTECHNOLOGY
  • 2011-10-15. MSClust: a tool for unsupervised mass spectra extraction of chromatography-mass spectrometry ion-wise aligned data in METABOLOMICS
  • 2011-09-28. Spectral trees as a robust annotation tool in LC–MS based metabolomics in METABOLOMICS
  • 2007-09-09. Metabolic engineering of flavonoids in tomato (Solanum lycopersicum): the potential for metabolomics in METABOLOMICS
  • 2010-05-13. Correlation Network Analysis reveals a sequential reorganization of metabolic and transcriptional states during germination and gene-metabolite relationships in developing seedlings of Arabidopsis in BMC SYSTEMS BIOLOGY
  • 2002-05-29. Effect of two vesicular-arbuscular mycorrhizal fungi on the growth of micropropagated potato plantlets and on the extent of disease caused by Rhizoctoniasolani in MYCORRHIZA
  • 2008-06-18. Time-resolved metabolomics reveals metabolic modulation in rice foliage in BMC SYSTEMS BIOLOGY
  • 2012-08-14. Secondary metabolites during early development in plants in PHYTOCHEMISTRY REVIEWS
  • 2010-04-29. Sinapate esters in brassicaceous plants: biochemistry, molecular biology, evolution and metabolic engineering in PLANTA
  • 2011-05-09. PHENOPSIS DB: an Information System for Arabidopsis thalianaphenotypic data in an environmental context in BMC PLANT BIOLOGY
  • 2007-09-12. Proposed minimum reporting standards for chemical analysis in METABOLOMICS
  • 2010-06-13. Ultraviolet A-specific induction of anthocyanin biosynthesis and PAL expression in tomato (Solanum lycopersicum L.) in PLANT GROWTH REGULATION
  • 2012-05-30. The tomato genome sequence provides insights into fleshy fruit evolution in NATURE
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