The platelet P2Y12 receptor under normal and pathological conditions. Assessment with the radiolabeled selective antagonist [3H]PSB-0413 View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2013-03

AUTHORS

Philippe Ohlmann, Anna Lecchi, Ali El-Tayeb, Christa E. Müller, Marco Cattaneo, Christian Gachet

ABSTRACT

Various radioligands have been used to characterize and quantify the platelet P2Y(12) receptor, which share several weaknesses: (a) they are metabolically unstable and substrates for ectoenzymes, (b) they are agonists, and (c) they do not discriminate between P2Y(1) and P2Y(12). We used the [(3)H]PSB-0413 selective P2Y(12) receptor antagonist radioligand to reevaluate the number of P2Y(12) receptors in intact platelets and in membrane preparations. Studies in humans showed that: (1) [(3)H]PSB-0413 bound to 425 ± 50 sites/platelet (K (D) = 3.3 ± 0.6 nM), (2) 0.5 ± 0.2 pmol [(3)H]PSB-0413 bound to 1 mg protein of platelet membranes (K (D) = 6.5 ± 3.6 nM), and (3) competition studies confirmed the known features of P2Y(12), with the expected rank order of potency: AR-C69931MX > 2MeSADP ≫ ADPβS > ADP, while the P2Y(1) ligand MRS2179 and the P2X(1) ligand α,β-Met-ATP did not displace [(3)H]PSB-0413 binding. Patients with severe P2Y(12) deficiency displayed virtually no binding of [(3)H]PSB-0413 to intact platelets, while a patient with a dysfunctional P2Y(12) receptor had normal binding. Studies in mice showed that: (1) [(3)H]PSB-0413 bound to 634 ± 87 sites/platelet (K (D) = 14 ± 4.5 nM) and (2) 0.7 pmol ± 0.3 [(3)H]PSB-0413 bound to 1 mg protein of platelet membranes (K (D) = 9.1 ± 5.3 nM). Clopidogrel and other thiol reagents like pCMBS or DTT abolished the binding both to intact platelets and membrane preparations. Therefore, [(3)H]PSB-0413 is an accurate and selective tool for radioligand binding studies aimed at quantifying P2Y(12) receptors, to identify patients with P2Y(12) deficiencies or quantify the effect of P2Y(12) targeting drugs. More... »

PAGES

59-66

References to SciGraph publications

  • 2011-09. Molecular defects of the platelet P2 receptors in PURINERGIC SIGNALLING
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s11302-012-9329-0

    DOI

    http://dx.doi.org/10.1007/s11302-012-9329-0

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1023522430

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/22892887


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biochemistry and Cell Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Adenosine Monophosphate", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Animals", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Binding, Competitive", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Blood Platelets", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Line, Tumor", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Membrane", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Data Interpretation, Statistical", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Humans", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mice", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Platelet Aggregation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Platelet Aggregation Inhibitors", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Purinergic P2X Receptor Antagonists", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Radioligand Assay", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Radiopharmaceuticals", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Receptors, Purinergic P2Y12", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Sulfhydryl Reagents", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Thionucleosides", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Ticlopidine", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Minist\u00e8re des Affaires sociales et de la Sant\u00e9", 
              "id": "https://www.grid.ac/institutes/grid.418199.c", 
              "name": [
                "UMR_S949 INSERM-Universit\u00e9 de Strasbourg, EFS-Alsace, 10 rue Spielmann, 67065, Strasbourg, France"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Ohlmann", 
            "givenName": "Philippe", 
            "id": "sg:person.01072562505.03", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01072562505.03"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico", 
              "id": "https://www.grid.ac/institutes/grid.414818.0", 
              "name": [
                "Angelo Bianchi Bonomi Hemophilia and Thrombosis Center, Department of Internal Medicine, Foundation IRCCS Ca\u2019Granda Ospedale Maggiore Policlinico and Universit\u00e0 degli Studi di Milano, Milan, Italy"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Lecchi", 
            "givenName": "Anna", 
            "id": "sg:person.01160221513.70", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01160221513.70"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Bonn", 
              "id": "https://www.grid.ac/institutes/grid.10388.32", 
              "name": [
                "PharmaCenter Bonn, Pharmaceutical Institute, Pharmaceutical Chemistry I, University of Bonn, An der Immenburg 4, 53121, Bonn, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "El-Tayeb", 
            "givenName": "Ali", 
            "id": "sg:person.01274771542.98", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01274771542.98"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Bonn", 
              "id": "https://www.grid.ac/institutes/grid.10388.32", 
              "name": [
                "PharmaCenter Bonn, Pharmaceutical Institute, Pharmaceutical Chemistry I, University of Bonn, An der Immenburg 4, 53121, Bonn, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "M\u00fcller", 
            "givenName": "Christa E.", 
            "id": "sg:person.010476717632.13", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010476717632.13"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Milan", 
              "id": "https://www.grid.ac/institutes/grid.4708.b", 
              "name": [
                "Unit\u00e0 di Medicina 3 Ospedale San Paolo, Dipartimento di Medcina, Chirurgia e Odontoiatra, Universit\u00e0 degli Studi di Milano, Milan, Italy"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Cattaneo", 
            "givenName": "Marco", 
            "id": "sg:person.0664323452.60", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0664323452.60"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Minist\u00e8re des Affaires sociales et de la Sant\u00e9", 
              "id": "https://www.grid.ac/institutes/grid.418199.c", 
              "name": [
                "UMR_S949 INSERM-Universit\u00e9 de Strasbourg, EFS-Alsace, 10 rue Spielmann, 67065, Strasbourg, France"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Gachet", 
            "givenName": "Christian", 
            "id": "sg:person.01224336740.17", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01224336740.17"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1016/j.bmcl.2005.08.104", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002582983"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.drudis.2010.05.011", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005329174"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1074/jbc.m801472200", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005940208"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0003-2697(80)90515-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007065635"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.phrs.2010.05.007", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008559063"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1146/annurev.pharmtox.46.120604.141207", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014387156"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1146/annurev.pharmtox.46.120604.141207", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014387156"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1182/blood-2002-10-3027", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014797573"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1182/blood-2004-07-2893", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014928080"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.phrs.2010.09.004", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014941420"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1538-7836.2004.00926.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014941546"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1042/bj2910875", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015561630"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1042/bj2910875", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015561630"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0304-4165(80)90394-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016703973"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0304-4165(80)90394-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016703973"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1124/mol.104.004846", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016960820"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1365-2125.2007.03044.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017552349"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1365-2141.1995.tb05319.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017810684"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1172/jci110786", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018201240"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1600-0854.2006.00469.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024530872"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1038/sj.bjp.0706453", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025390856"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1038/sj.bjp.0706453", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025390856"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1161/01.cir.0000085073.69189.88", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1027857322"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1538-7836.2005.01483.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028240916"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1538-7836.2005.01483.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028240916"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0014-2999(01)00733-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029609474"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0076-6879(92)15049-i", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030379980"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1161/01.atv.20.11.e101", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033640375"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1182/blood-2011-02-336826", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036134311"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1152/physrev.00043.2006", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038418702"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1182/blood-2010-08-263111", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041486740"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0076-6879(92)15059-l", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042379591"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.ijcard.2007.12.118", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047773818"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1161/circulationaha.109.853069", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051357544"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.0437879100", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051735130"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1161/01.cir.0000106904.80795.35", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1052121904"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s11302-011-9217-z", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053556806", 
              "https://doi.org/10.1007/s11302-011-9217-z"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/jm9003297", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055956406"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/jm9003297", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055956406"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1055/s-2005-869523", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1057447349"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1160/th07-11-0673", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1063291447"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1160/th11-06-0430", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1063292775"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1160/ths10-11-0742", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1063294432"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1055/s-0037-1614049", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1074708833"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1075647865", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1076859391", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1079692304", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1081112291", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1082251437", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1082690237", 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2013-03", 
        "datePublishedReg": "2013-03-01", 
        "description": "Various radioligands have been used to characterize and quantify the platelet P2Y(12) receptor, which share several weaknesses: (a) they are metabolically unstable and substrates for ectoenzymes, (b) they are agonists, and (c) they do not discriminate between P2Y(1) and P2Y(12). We used the [(3)H]PSB-0413 selective P2Y(12) receptor antagonist radioligand to reevaluate the number of P2Y(12) receptors in intact platelets and in membrane preparations. Studies in humans showed that: (1) [(3)H]PSB-0413 bound to 425\u2009\u00b1\u200950 sites/platelet (K                 (D)\u2009=\u20093.3\u2009\u00b1\u20090.6\u00a0nM), (2) 0.5\u2009\u00b1\u20090.2\u00a0pmol [(3)H]PSB-0413 bound to 1\u00a0mg protein of platelet membranes (K                 (D)\u2009=\u20096.5\u2009\u00b1\u20093.6\u00a0nM), and (3) competition studies confirmed the known features of P2Y(12), with the expected rank order of potency: AR-C69931MX\u2009>\u20092MeSADP\u2009\u226b\u2009ADP\u03b2S\u2009>\u2009ADP, while the P2Y(1) ligand MRS2179 and the P2X(1) ligand \u03b1,\u03b2-Met-ATP did not displace [(3)H]PSB-0413 binding. Patients with severe P2Y(12) deficiency displayed virtually no binding of [(3)H]PSB-0413 to intact platelets, while a patient with a dysfunctional P2Y(12) receptor had normal binding. Studies in mice showed that: (1) [(3)H]PSB-0413 bound to 634\u2009\u00b1\u200987 sites/platelet (K                 (D)\u2009=\u200914\u2009\u00b1\u20094.5\u00a0nM) and (2) 0.7\u00a0pmol\u2009\u00b1\u20090.3 [(3)H]PSB-0413 bound to 1\u00a0mg protein of platelet membranes (K                 (D)\u2009=\u20099.1\u2009\u00b1\u20095.3\u00a0nM). Clopidogrel and other thiol reagents like pCMBS or DTT abolished the binding both to intact platelets and membrane preparations. Therefore, [(3)H]PSB-0413 is an accurate and selective tool for radioligand binding studies aimed at quantifying P2Y(12) receptors, to identify patients with P2Y(12) deficiencies or quantify the effect of P2Y(12) targeting drugs.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1007/s11302-012-9329-0", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": true, 
        "isPartOf": [
          {
            "id": "sg:journal.1035517", 
            "issn": [
              "1573-9538", 
              "1573-9546"
            ], 
            "name": "Purinergic Signalling", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "9"
          }
        ], 
        "name": "The platelet P2Y12 receptor under normal and pathological conditions. Assessment with the radiolabeled selective antagonist [3H]PSB-0413", 
        "pagination": "59-66", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "701140630385b293bfd0433c1b58b47ab13b9182580095b0102066f7e593d6e0"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "22892887"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "101250499"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/s11302-012-9329-0"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1023522430"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/s11302-012-9329-0", 
          "https://app.dimensions.ai/details/publication/pub.1023522430"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-10T16:46", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8669_00000532.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://link.springer.com/10.1007%2Fs11302-012-9329-0"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s11302-012-9329-0'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s11302-012-9329-0'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s11302-012-9329-0'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s11302-012-9329-0'


     

    This table displays all metadata directly associated to this object as RDF triples.

    312 TRIPLES      21 PREDICATES      91 URIs      39 LITERALS      27 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/s11302-012-9329-0 schema:about N08ec5d33d1e74c0da831c983744e1012
    2 N0a27106e070246beb7b3ec1bf78113f4
    3 N0ee64fb49f564327ada5d92f5198813a
    4 N1d8264ee83c54d3e882eefc64a6e34fe
    5 N2cafb2cfddcd4ae8b4ec98a1b63163ba
    6 N45d5cb6d3ff9431eae242e5c69f57403
    7 N687377035825486eac7a693a769e3f40
    8 N71d30f4c92934b68b3cc71b64c3ff6af
    9 N7dcfdac2b8714f578ae6cd25422a653a
    10 N7e41eb4590a24ab099c27adfbb66b2f3
    11 N87843440156d4818bb19b400331c048c
    12 N9718f90927ca42669785d8576c3af9c6
    13 N9b7b5ff064a342e89cfa70d2e5481940
    14 Nb79a00d987194015b3ab04abd9ee5056
    15 Nba38d48c3865407fb0275fa301d285be
    16 Nce1f506e875f42dcba2e0764e1040960
    17 Nd52b4b0179d0412bba67db9235096920
    18 Nd82f20ae253d458b964bfd57f3f909ce
    19 anzsrc-for:06
    20 anzsrc-for:0601
    21 schema:author N64216ab92fa9452b9841efbd9440ef2a
    22 schema:citation sg:pub.10.1007/s11302-011-9217-z
    23 https://app.dimensions.ai/details/publication/pub.1075647865
    24 https://app.dimensions.ai/details/publication/pub.1076859391
    25 https://app.dimensions.ai/details/publication/pub.1079692304
    26 https://app.dimensions.ai/details/publication/pub.1081112291
    27 https://app.dimensions.ai/details/publication/pub.1082251437
    28 https://app.dimensions.ai/details/publication/pub.1082690237
    29 https://doi.org/10.1016/0003-2697(80)90515-1
    30 https://doi.org/10.1016/0076-6879(92)15049-i
    31 https://doi.org/10.1016/0076-6879(92)15059-l
    32 https://doi.org/10.1016/0304-4165(80)90394-3
    33 https://doi.org/10.1016/j.bmcl.2005.08.104
    34 https://doi.org/10.1016/j.drudis.2010.05.011
    35 https://doi.org/10.1016/j.ijcard.2007.12.118
    36 https://doi.org/10.1016/j.phrs.2010.05.007
    37 https://doi.org/10.1016/j.phrs.2010.09.004
    38 https://doi.org/10.1016/s0014-2999(01)00733-6
    39 https://doi.org/10.1021/jm9003297
    40 https://doi.org/10.1038/sj.bjp.0706453
    41 https://doi.org/10.1042/bj2910875
    42 https://doi.org/10.1055/s-0037-1614049
    43 https://doi.org/10.1055/s-2005-869523
    44 https://doi.org/10.1073/pnas.0437879100
    45 https://doi.org/10.1074/jbc.m801472200
    46 https://doi.org/10.1111/j.1365-2125.2007.03044.x
    47 https://doi.org/10.1111/j.1365-2141.1995.tb05319.x
    48 https://doi.org/10.1111/j.1538-7836.2004.00926.x
    49 https://doi.org/10.1111/j.1538-7836.2005.01483.x
    50 https://doi.org/10.1111/j.1600-0854.2006.00469.x
    51 https://doi.org/10.1124/mol.104.004846
    52 https://doi.org/10.1146/annurev.pharmtox.46.120604.141207
    53 https://doi.org/10.1152/physrev.00043.2006
    54 https://doi.org/10.1160/th07-11-0673
    55 https://doi.org/10.1160/th11-06-0430
    56 https://doi.org/10.1160/ths10-11-0742
    57 https://doi.org/10.1161/01.atv.20.11.e101
    58 https://doi.org/10.1161/01.cir.0000085073.69189.88
    59 https://doi.org/10.1161/01.cir.0000106904.80795.35
    60 https://doi.org/10.1161/circulationaha.109.853069
    61 https://doi.org/10.1172/jci110786
    62 https://doi.org/10.1182/blood-2002-10-3027
    63 https://doi.org/10.1182/blood-2004-07-2893
    64 https://doi.org/10.1182/blood-2010-08-263111
    65 https://doi.org/10.1182/blood-2011-02-336826
    66 schema:datePublished 2013-03
    67 schema:datePublishedReg 2013-03-01
    68 schema:description Various radioligands have been used to characterize and quantify the platelet P2Y(12) receptor, which share several weaknesses: (a) they are metabolically unstable and substrates for ectoenzymes, (b) they are agonists, and (c) they do not discriminate between P2Y(1) and P2Y(12). We used the [(3)H]PSB-0413 selective P2Y(12) receptor antagonist radioligand to reevaluate the number of P2Y(12) receptors in intact platelets and in membrane preparations. Studies in humans showed that: (1) [(3)H]PSB-0413 bound to 425 ± 50 sites/platelet (K (D) = 3.3 ± 0.6 nM), (2) 0.5 ± 0.2 pmol [(3)H]PSB-0413 bound to 1 mg protein of platelet membranes (K (D) = 6.5 ± 3.6 nM), and (3) competition studies confirmed the known features of P2Y(12), with the expected rank order of potency: AR-C69931MX > 2MeSADP ≫ ADPβS > ADP, while the P2Y(1) ligand MRS2179 and the P2X(1) ligand α,β-Met-ATP did not displace [(3)H]PSB-0413 binding. Patients with severe P2Y(12) deficiency displayed virtually no binding of [(3)H]PSB-0413 to intact platelets, while a patient with a dysfunctional P2Y(12) receptor had normal binding. Studies in mice showed that: (1) [(3)H]PSB-0413 bound to 634 ± 87 sites/platelet (K (D) = 14 ± 4.5 nM) and (2) 0.7 pmol ± 0.3 [(3)H]PSB-0413 bound to 1 mg protein of platelet membranes (K (D) = 9.1 ± 5.3 nM). Clopidogrel and other thiol reagents like pCMBS or DTT abolished the binding both to intact platelets and membrane preparations. Therefore, [(3)H]PSB-0413 is an accurate and selective tool for radioligand binding studies aimed at quantifying P2Y(12) receptors, to identify patients with P2Y(12) deficiencies or quantify the effect of P2Y(12) targeting drugs.
    69 schema:genre research_article
    70 schema:inLanguage en
    71 schema:isAccessibleForFree true
    72 schema:isPartOf Nc81b73be16ae43a197f60e2c5e79754e
    73 Ncb831120752548fd859444770da85985
    74 sg:journal.1035517
    75 schema:name The platelet P2Y12 receptor under normal and pathological conditions. Assessment with the radiolabeled selective antagonist [3H]PSB-0413
    76 schema:pagination 59-66
    77 schema:productId N1907931598f24035857d76043b141bc3
    78 N43be79b5b7ff40fd9defe6f8930d5f2d
    79 N56776127b77b41699bcf99319240a59d
    80 N77f8d92afab4446e9cd4139bf79e6128
    81 Necc9f57fb2484526882fb56fbcca9064
    82 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023522430
    83 https://doi.org/10.1007/s11302-012-9329-0
    84 schema:sdDatePublished 2019-04-10T16:46
    85 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    86 schema:sdPublisher N04382891a52e4d059f0b6d1056693552
    87 schema:url http://link.springer.com/10.1007%2Fs11302-012-9329-0
    88 sgo:license sg:explorer/license/
    89 sgo:sdDataset articles
    90 rdf:type schema:ScholarlyArticle
    91 N04382891a52e4d059f0b6d1056693552 schema:name Springer Nature - SN SciGraph project
    92 rdf:type schema:Organization
    93 N08ec5d33d1e74c0da831c983744e1012 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    94 schema:name Blood Platelets
    95 rdf:type schema:DefinedTerm
    96 N0a27106e070246beb7b3ec1bf78113f4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    97 schema:name Platelet Aggregation Inhibitors
    98 rdf:type schema:DefinedTerm
    99 N0ee64fb49f564327ada5d92f5198813a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    100 schema:name Radiopharmaceuticals
    101 rdf:type schema:DefinedTerm
    102 N1907931598f24035857d76043b141bc3 schema:name dimensions_id
    103 schema:value pub.1023522430
    104 rdf:type schema:PropertyValue
    105 N1d8264ee83c54d3e882eefc64a6e34fe schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    106 schema:name Thionucleosides
    107 rdf:type schema:DefinedTerm
    108 N2cafb2cfddcd4ae8b4ec98a1b63163ba schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    109 schema:name Animals
    110 rdf:type schema:DefinedTerm
    111 N3984cb2ed9814228b18d90e7a15e3ee8 rdf:first sg:person.01160221513.70
    112 rdf:rest N3bb8aa6a7d8441ba810647f09529609b
    113 N3bb8aa6a7d8441ba810647f09529609b rdf:first sg:person.01274771542.98
    114 rdf:rest Nd62f2a82755b457e93bdf1a8ad880b63
    115 N43be79b5b7ff40fd9defe6f8930d5f2d schema:name doi
    116 schema:value 10.1007/s11302-012-9329-0
    117 rdf:type schema:PropertyValue
    118 N45d5cb6d3ff9431eae242e5c69f57403 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    119 schema:name Sulfhydryl Reagents
    120 rdf:type schema:DefinedTerm
    121 N56776127b77b41699bcf99319240a59d schema:name nlm_unique_id
    122 schema:value 101250499
    123 rdf:type schema:PropertyValue
    124 N64216ab92fa9452b9841efbd9440ef2a rdf:first sg:person.01072562505.03
    125 rdf:rest N3984cb2ed9814228b18d90e7a15e3ee8
    126 N687377035825486eac7a693a769e3f40 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    127 schema:name Purinergic P2X Receptor Antagonists
    128 rdf:type schema:DefinedTerm
    129 N6abe6a4325e2487ebcaf6bd1516cd9a2 rdf:first sg:person.01224336740.17
    130 rdf:rest rdf:nil
    131 N71d30f4c92934b68b3cc71b64c3ff6af schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    132 schema:name Platelet Aggregation
    133 rdf:type schema:DefinedTerm
    134 N77f8d92afab4446e9cd4139bf79e6128 schema:name pubmed_id
    135 schema:value 22892887
    136 rdf:type schema:PropertyValue
    137 N7dcfdac2b8714f578ae6cd25422a653a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    138 schema:name Cell Line, Tumor
    139 rdf:type schema:DefinedTerm
    140 N7e41eb4590a24ab099c27adfbb66b2f3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    141 schema:name Receptors, Purinergic P2Y12
    142 rdf:type schema:DefinedTerm
    143 N87843440156d4818bb19b400331c048c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    144 schema:name Adenosine Monophosphate
    145 rdf:type schema:DefinedTerm
    146 N9718f90927ca42669785d8576c3af9c6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    147 schema:name Mice
    148 rdf:type schema:DefinedTerm
    149 N9b7b5ff064a342e89cfa70d2e5481940 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    150 schema:name Radioligand Assay
    151 rdf:type schema:DefinedTerm
    152 Nb79a00d987194015b3ab04abd9ee5056 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    153 schema:name Humans
    154 rdf:type schema:DefinedTerm
    155 Nba38d48c3865407fb0275fa301d285be schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    156 schema:name Cell Membrane
    157 rdf:type schema:DefinedTerm
    158 Nc81b73be16ae43a197f60e2c5e79754e schema:issueNumber 1
    159 rdf:type schema:PublicationIssue
    160 Ncb831120752548fd859444770da85985 schema:volumeNumber 9
    161 rdf:type schema:PublicationVolume
    162 Nce1f506e875f42dcba2e0764e1040960 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    163 schema:name Binding, Competitive
    164 rdf:type schema:DefinedTerm
    165 Nd52b4b0179d0412bba67db9235096920 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    166 schema:name Ticlopidine
    167 rdf:type schema:DefinedTerm
    168 Nd62f2a82755b457e93bdf1a8ad880b63 rdf:first sg:person.010476717632.13
    169 rdf:rest Nfa664007c286458c86afc051d7bf0b08
    170 Nd82f20ae253d458b964bfd57f3f909ce schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    171 schema:name Data Interpretation, Statistical
    172 rdf:type schema:DefinedTerm
    173 Necc9f57fb2484526882fb56fbcca9064 schema:name readcube_id
    174 schema:value 701140630385b293bfd0433c1b58b47ab13b9182580095b0102066f7e593d6e0
    175 rdf:type schema:PropertyValue
    176 Nfa664007c286458c86afc051d7bf0b08 rdf:first sg:person.0664323452.60
    177 rdf:rest N6abe6a4325e2487ebcaf6bd1516cd9a2
    178 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    179 schema:name Biological Sciences
    180 rdf:type schema:DefinedTerm
    181 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
    182 schema:name Biochemistry and Cell Biology
    183 rdf:type schema:DefinedTerm
    184 sg:journal.1035517 schema:issn 1573-9538
    185 1573-9546
    186 schema:name Purinergic Signalling
    187 rdf:type schema:Periodical
    188 sg:person.010476717632.13 schema:affiliation https://www.grid.ac/institutes/grid.10388.32
    189 schema:familyName Müller
    190 schema:givenName Christa E.
    191 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010476717632.13
    192 rdf:type schema:Person
    193 sg:person.01072562505.03 schema:affiliation https://www.grid.ac/institutes/grid.418199.c
    194 schema:familyName Ohlmann
    195 schema:givenName Philippe
    196 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01072562505.03
    197 rdf:type schema:Person
    198 sg:person.01160221513.70 schema:affiliation https://www.grid.ac/institutes/grid.414818.0
    199 schema:familyName Lecchi
    200 schema:givenName Anna
    201 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01160221513.70
    202 rdf:type schema:Person
    203 sg:person.01224336740.17 schema:affiliation https://www.grid.ac/institutes/grid.418199.c
    204 schema:familyName Gachet
    205 schema:givenName Christian
    206 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01224336740.17
    207 rdf:type schema:Person
    208 sg:person.01274771542.98 schema:affiliation https://www.grid.ac/institutes/grid.10388.32
    209 schema:familyName El-Tayeb
    210 schema:givenName Ali
    211 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01274771542.98
    212 rdf:type schema:Person
    213 sg:person.0664323452.60 schema:affiliation https://www.grid.ac/institutes/grid.4708.b
    214 schema:familyName Cattaneo
    215 schema:givenName Marco
    216 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0664323452.60
    217 rdf:type schema:Person
    218 sg:pub.10.1007/s11302-011-9217-z schema:sameAs https://app.dimensions.ai/details/publication/pub.1053556806
    219 https://doi.org/10.1007/s11302-011-9217-z
    220 rdf:type schema:CreativeWork
    221 https://app.dimensions.ai/details/publication/pub.1075647865 schema:CreativeWork
    222 https://app.dimensions.ai/details/publication/pub.1076859391 schema:CreativeWork
    223 https://app.dimensions.ai/details/publication/pub.1079692304 schema:CreativeWork
    224 https://app.dimensions.ai/details/publication/pub.1081112291 schema:CreativeWork
    225 https://app.dimensions.ai/details/publication/pub.1082251437 schema:CreativeWork
    226 https://app.dimensions.ai/details/publication/pub.1082690237 schema:CreativeWork
    227 https://doi.org/10.1016/0003-2697(80)90515-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007065635
    228 rdf:type schema:CreativeWork
    229 https://doi.org/10.1016/0076-6879(92)15049-i schema:sameAs https://app.dimensions.ai/details/publication/pub.1030379980
    230 rdf:type schema:CreativeWork
    231 https://doi.org/10.1016/0076-6879(92)15059-l schema:sameAs https://app.dimensions.ai/details/publication/pub.1042379591
    232 rdf:type schema:CreativeWork
    233 https://doi.org/10.1016/0304-4165(80)90394-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016703973
    234 rdf:type schema:CreativeWork
    235 https://doi.org/10.1016/j.bmcl.2005.08.104 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002582983
    236 rdf:type schema:CreativeWork
    237 https://doi.org/10.1016/j.drudis.2010.05.011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005329174
    238 rdf:type schema:CreativeWork
    239 https://doi.org/10.1016/j.ijcard.2007.12.118 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047773818
    240 rdf:type schema:CreativeWork
    241 https://doi.org/10.1016/j.phrs.2010.05.007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008559063
    242 rdf:type schema:CreativeWork
    243 https://doi.org/10.1016/j.phrs.2010.09.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014941420
    244 rdf:type schema:CreativeWork
    245 https://doi.org/10.1016/s0014-2999(01)00733-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029609474
    246 rdf:type schema:CreativeWork
    247 https://doi.org/10.1021/jm9003297 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055956406
    248 rdf:type schema:CreativeWork
    249 https://doi.org/10.1038/sj.bjp.0706453 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025390856
    250 rdf:type schema:CreativeWork
    251 https://doi.org/10.1042/bj2910875 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015561630
    252 rdf:type schema:CreativeWork
    253 https://doi.org/10.1055/s-0037-1614049 schema:sameAs https://app.dimensions.ai/details/publication/pub.1074708833
    254 rdf:type schema:CreativeWork
    255 https://doi.org/10.1055/s-2005-869523 schema:sameAs https://app.dimensions.ai/details/publication/pub.1057447349
    256 rdf:type schema:CreativeWork
    257 https://doi.org/10.1073/pnas.0437879100 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051735130
    258 rdf:type schema:CreativeWork
    259 https://doi.org/10.1074/jbc.m801472200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005940208
    260 rdf:type schema:CreativeWork
    261 https://doi.org/10.1111/j.1365-2125.2007.03044.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1017552349
    262 rdf:type schema:CreativeWork
    263 https://doi.org/10.1111/j.1365-2141.1995.tb05319.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1017810684
    264 rdf:type schema:CreativeWork
    265 https://doi.org/10.1111/j.1538-7836.2004.00926.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1014941546
    266 rdf:type schema:CreativeWork
    267 https://doi.org/10.1111/j.1538-7836.2005.01483.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1028240916
    268 rdf:type schema:CreativeWork
    269 https://doi.org/10.1111/j.1600-0854.2006.00469.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1024530872
    270 rdf:type schema:CreativeWork
    271 https://doi.org/10.1124/mol.104.004846 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016960820
    272 rdf:type schema:CreativeWork
    273 https://doi.org/10.1146/annurev.pharmtox.46.120604.141207 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014387156
    274 rdf:type schema:CreativeWork
    275 https://doi.org/10.1152/physrev.00043.2006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038418702
    276 rdf:type schema:CreativeWork
    277 https://doi.org/10.1160/th07-11-0673 schema:sameAs https://app.dimensions.ai/details/publication/pub.1063291447
    278 rdf:type schema:CreativeWork
    279 https://doi.org/10.1160/th11-06-0430 schema:sameAs https://app.dimensions.ai/details/publication/pub.1063292775
    280 rdf:type schema:CreativeWork
    281 https://doi.org/10.1160/ths10-11-0742 schema:sameAs https://app.dimensions.ai/details/publication/pub.1063294432
    282 rdf:type schema:CreativeWork
    283 https://doi.org/10.1161/01.atv.20.11.e101 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033640375
    284 rdf:type schema:CreativeWork
    285 https://doi.org/10.1161/01.cir.0000085073.69189.88 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027857322
    286 rdf:type schema:CreativeWork
    287 https://doi.org/10.1161/01.cir.0000106904.80795.35 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052121904
    288 rdf:type schema:CreativeWork
    289 https://doi.org/10.1161/circulationaha.109.853069 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051357544
    290 rdf:type schema:CreativeWork
    291 https://doi.org/10.1172/jci110786 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018201240
    292 rdf:type schema:CreativeWork
    293 https://doi.org/10.1182/blood-2002-10-3027 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014797573
    294 rdf:type schema:CreativeWork
    295 https://doi.org/10.1182/blood-2004-07-2893 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014928080
    296 rdf:type schema:CreativeWork
    297 https://doi.org/10.1182/blood-2010-08-263111 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041486740
    298 rdf:type schema:CreativeWork
    299 https://doi.org/10.1182/blood-2011-02-336826 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036134311
    300 rdf:type schema:CreativeWork
    301 https://www.grid.ac/institutes/grid.10388.32 schema:alternateName University of Bonn
    302 schema:name PharmaCenter Bonn, Pharmaceutical Institute, Pharmaceutical Chemistry I, University of Bonn, An der Immenburg 4, 53121, Bonn, Germany
    303 rdf:type schema:Organization
    304 https://www.grid.ac/institutes/grid.414818.0 schema:alternateName Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico
    305 schema:name Angelo Bianchi Bonomi Hemophilia and Thrombosis Center, Department of Internal Medicine, Foundation IRCCS Ca’Granda Ospedale Maggiore Policlinico and Università degli Studi di Milano, Milan, Italy
    306 rdf:type schema:Organization
    307 https://www.grid.ac/institutes/grid.418199.c schema:alternateName Ministère des Affaires sociales et de la Santé
    308 schema:name UMR_S949 INSERM-Université de Strasbourg, EFS-Alsace, 10 rue Spielmann, 67065, Strasbourg, France
    309 rdf:type schema:Organization
    310 https://www.grid.ac/institutes/grid.4708.b schema:alternateName University of Milan
    311 schema:name Unità di Medicina 3 Ospedale San Paolo, Dipartimento di Medcina, Chirurgia e Odontoiatra, Università degli Studi di Milano, Milan, Italy
    312 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...